Restriction map for
pF1KB6344
(Circular) (Six-base) MAPSORT of: pF1KB6344.seq Check: 6760 from: 1 to: 3986
pF1KB6344 3986 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 728 1024 728
Size: 296 3690
Acc65I G'GTAC_C
Cuts at: 1002 1002
Size: 3986
AclI AA'CG_TT
Cuts at: 3826 3826
Size: 3986
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1811 2243 2990 3258 262
Size: 1549 432 747 268 990
Fragments arranged by size:
1549 990 747 432 268
AfeI AGC'GCT
Cuts at: 484 484
Size: 3986
AflIII A'CryG_T
Cuts at: 1338 2442 3419 1338
Size: 1104 977 1905
Fragments arranged by size:
1905 1104 977
AgeI A'CCGG_T
Cuts at: 2374 2374
Size: 3986
AlwNI CAG_nnn'CTG
Cuts at: 272 945 1291 2858 272
Size: 673 346 1567 1400
Fragments arranged by size:
1567 1400 673 346
ApaLI G'TGCA_C
Cuts at: 357 2756 357
Size: 2399 1587
ApoI r'AATT_y
Cuts at: 655 990 3339 3595 655
Size: 335 2349 256 1046
Fragments arranged by size:
2349 1046 335 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
AvaI C'yCGr_G
Cuts at: 569 1006 569
Size: 437 3549
BamHI G'GATC_C
Cuts at: 1011 1011
Size: 3986
BanI G'GyrC_C
Cuts at: 249 649 1002 1416 1664 1699 249
Size: 400 353 414 248 35 2536
Fragments arranged by size:
2536 414 400 353 248 35
BanII G_rGCy'C
Cuts at: 116 1000 2030 116
Size: 884 1030 2072
Fragments arranged by size:
2072 1030 884
BbeI G_GCGC'C
Cuts at: 253 1420 1668 253
Size: 1167 248 2571
Fragments arranged by size:
2571 1167 248
BbsI GAAGACnn'nnnn_
Cuts at: 268 268
Size: 3986
BciVI GTATCCnnnnn_n'
Cuts at: 1884 2645 3986 1884
Size: 761 1341 1884
Fragments arranged by size:
1884 1341 761
BclI T'GATC_A
Cuts at: 1506 1506
Size: 3986
BglI GCCn_nnn'nGGC
Cuts at: 133 133
Size: 3986
BglII A'GATC_T
Cuts at: 1501 3538 1501
Size: 2037 1949
BlpI GC'TnA_GC
Cuts at: 1098 1098
Size: 3986
Bme1580I G_kGCm'C
Cuts at: 361 1611 1704 2760 361
Size: 1250 93 1056 1587
Fragments arranged by size:
1587 1250 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 967 1198 1279 1614 3758 967
Size: 231 81 335 2144 1195
Fragments arranged by size:
2144 1195 335 231 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 781 2353 781
Size: 1572 2414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 697 922 178
Size: 519 225 3242
Fragments arranged by size:
3242 519 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1162 2041 2533 2831 1162
Size: 879 492 298 2317
Fragments arranged by size:
2317 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3690 3690
Size: 3986
BsaAI yAC'GTr
Cuts at: 1339 1339
Size: 3986
BsaBI GATnn'nnATC
Cuts at: 6 1500 3537 6
Size: 1494 2037 455
Fragments arranged by size:
2037 1494 455
BsaHI Gr'CG_yC
Cuts at: 250 1417 1665 2367 3863 250
Size: 1167 248 702 1496 373
Fragments arranged by size:
1496 1167 702 373 248
BsaWI w'CCGG_w
Cuts at: 413 1175 1696 2374 2648 2795 3206 413
Size: 762 521 678 274 147 411 1193
Fragments arranged by size:
1193 762 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 232
Size: 3986
BseYI C'CCAG_C
Cuts at: 200 538 594 1411 2169 2746 3242 200
Size: 338 56 817 758 577 496 944
Fragments arranged by size:
944 817 758 577 496 338 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 910
Size: 3986
BsiEI CG_ry'CG
Cuts at: 104 1528 1574 2782 104
Size: 1424 46 1208 1308
Fragments arranged by size:
1424 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 361 1000 1778 1968 2760 361
Size: 639 778 190 792 1587
Fragments arranged by size:
1587 792 778 639 190
BsiWI C'GTAC_G
Cuts at: 560 560
Size: 3986
BsmI GAATG_Cn'
Cuts at: 621 621
Size: 3986
BsmBI CGTCTCn'nnnn_
Cuts at: 961 961
Size: 3986
Bsp1286I G_dGCh'C
Cuts at: 116 361 1000 1611 1704 1778 1968 2030 2760
Size: 245 639 611 93 74 190 62 730
Cuts at: 2760 116
Size: 1342
Fragments arranged by size:
1342 730 639 611 245 190 93 74
62
BspEI T'CCGG_A
Cuts at: 413 3206 413
Size: 2793 1193
BspHI T'CATG_A
Cuts at: 845 3162 845
Size: 2317 1669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1036 1250 1552 1933 1036
Size: 214 302 381 3089
Fragments arranged by size:
3089 381 302 214
BsrBI CCG'CTC
Cuts at: 50 818 2278 2332 3816 3981 50
Size: 768 1460 54 1484 165 55
Fragments arranged by size:
1484 1460 768 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1898 1898
Size: 3986
BsrFI r'CCGG_y
Cuts at: 328 1984 2165 2374 328
Size: 1656 181 209 1940
Fragments arranged by size:
1940 1656 209 181
BssSI C'ACGA_G
Cuts at: 2257 2615 2257
Size: 358 3628
BstAPI GCAn_nnn'nTGC
Cuts at: 341 486 908 1363 341
Size: 145 422 455 2964
Fragments arranged by size:
2964 455 422 145
BstBI TT'CG_AA
Cuts at: 2347 2347
Size: 3986
BstYI r'GATC_y
Cuts at: 1 425 1011 1478 1501 1836 2082 3094 3180
Size: 424 586 467 23 335 246 1012 86
Cuts at: 3180 3192 3538 1
Size: 12 346 449
Fragments arranged by size:
1012 586 467 449 424 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 429 663 803 107
Size: 322 234 140 3290
Fragments arranged by size:
3290 322 234 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1936 2117 2280 1936
Size: 181 163 3642
Fragments arranged by size:
3642 181 163
BtsI GCAGTG_nn'
Cuts at: 394 1229 394
Size: 835 3151
ClaI AT'CG_AT
Cuts at: 524 524
Size: 3986
DraI TTT'AAA
Cuts at: 985 985
Size: 3986
DraIII CAC_nnn'GTG
Cuts at: 612 612
Size: 3986
DrdI GACnn_nn'nnGTC
Cuts at: 724 1692 2550 724
Size: 968 858 2160
Fragments arranged by size:
2160 968 858
EaeI y'GGCC_r
Cuts at: 1571 1745 2136 2163 3508 1571
Size: 174 391 27 1345 2049
Fragments arranged by size:
2049 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1571 1571
Size: 3986
EarI CTCTTCn'nnn_
Cuts at: 550 2009 2219 550
Size: 1459 210 2317
Fragments arranged by size:
2317 1459 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 190 712 2194 2504 2650 3796 190
Size: 522 1482 310 146 1146 380
Fragments arranged by size:
1482 1146 522 380 310 146
EcoICRI GAG'CTC
Cuts at: 998 998
Size: 3986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 262 697 922 1811 2243 2990 3258 178
Size: 84 435 225 889 432 747 268 906
Fragments arranged by size:
906 889 747 435 432 268 225 84
EcoNI CCTnn'n_nnAGG
Cuts at: 917 917
Size: 3986
EcoO109I rG'GnC_Cy
Cuts at: 408 1125 408
Size: 717 3269
EcoRI G'AATT_C
Cuts at: 990 990
Size: 3986
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3986
FspI TGC'GCA
Cuts at: 1767 1767
Size: 3986
HaeII r_GCGC'y
Cuts at: 253 486 1420 1668 2690 3669 253
Size: 233 934 248 1022 979 570
Fragments arranged by size:
1022 979 934 570 248 233
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1828 1858 1860 1890 1828
Size: 30 2 30 3924
Fragments arranged by size:
3924 30 30 2
HincII GTy'rAC
Cuts at: 1025 1025
Size: 3986
KasI G'GCGC_C
Cuts at: 249 1416 1664 249
Size: 1167 248 2571
Fragments arranged by size:
2571 1167 248
KpnI G_GTAC'C
Cuts at: 1006 1006
Size: 3986
MluI A'CGCG_T
Cuts at: 3419 3419
Size: 3986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2657 2841 2657
Size: 184 3802
MscI TGG'CCA
Cuts at: 1747 1747
Size: 3986
MslI CAynn'nnrTG
Cuts at: 524 1975 2102 524
Size: 1451 127 2408
Fragments arranged by size:
2408 1451 127
MspA1I CmG'CkG
Cuts at: 594 622 1097 1411 1771 2784 3029 594
Size: 28 475 314 360 1013 245 1551
Fragments arranged by size:
1551 1013 475 360 314 245 28
NaeI GCC'GGC
Cuts at: 330 2167 330
Size: 1837 2149
NarI GG'CG_CC
Cuts at: 250 1417 1665 250
Size: 1167 248 2571
Fragments arranged by size:
2571 1167 248
NcoI C'CATG_G
Cuts at: 107 429 803 107
Size: 322 374 3290
Fragments arranged by size:
3290 374 322
NgoMIV G'CCGG_C
Cuts at: 328 2165 328
Size: 1837 2149
NspI r_CATG'y
Cuts at: 1039 1539 2446 1039
Size: 500 907 2579
Fragments arranged by size:
2579 907 500
PciI A'CATG_T
Cuts at: 2442 2442
Size: 3986
PflMI CCAn_nnn'nTGG
Cuts at: 387 945 387
Size: 558 3428
PmeI GTTT'AAAC
Cuts at: 985 985
Size: 3986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 3954
PpuMI rG'GwC_Cy
Cuts at: 408 408
Size: 3986
PstI C_TGCA'G
Cuts at: 401 482 627 1033 1718 401
Size: 81 145 406 685 2669
Fragments arranged by size:
2669 685 406 145 81
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3986
PvuII CAG'CTG
Cuts at: 594 1411 1771 594
Size: 817 360 2809
Fragments arranged by size:
2809 817 360
RsrII CG'GwC_CG
Cuts at: 2181 2181
Size: 3986
SacI G_AGCT'C
Cuts at: 1000 1000
Size: 3986
SalI G'TCGA_C
Cuts at: 1023 1023
Size: 3986
SapI GCTCTTCn'nnn_
Cuts at: 2009 2219 2009
Size: 210 3776
SbfI CC_TGCA'GG
Cuts at: 1033 1033
Size: 3986
ScaI AGT'ACT
Cuts at: 682 3503 682
Size: 2821 1165
SfcI C'TryA_G
Cuts at: 33 397 478 623 1029 1714 2707 2898 33
Size: 364 81 145 406 685 993 191 1121
Fragments arranged by size:
1121 993 685 406 364 191 145 81
SfoI GGC'GCC
Cuts at: 251 1418 1666 251
Size: 1167 248 2571
Fragments arranged by size:
2571 1167 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
SmaI CCC'GGG
Cuts at: 571 1008 571
Size: 437 3549
SmlI C'TyrA_G
Cuts at: 1141 2056 2548 2810 1141
Size: 915 492 262 2317
Fragments arranged by size:
2317 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3986
SphI G_CATG'C
Cuts at: 1039 1539 1039
Size: 500 3486
StyI C'CwwG_G
Cuts at: 107 429 777 803 1120 1474 107
Size: 322 348 26 317 354 2619
Fragments arranged by size:
2619 354 348 322 317 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 619 1569 1702 2161 2370 619
Size: 950 133 459 209 2235
Fragments arranged by size:
2235 950 459 209 133
TatI w'GTAC_w
Cuts at: 680 3501 680
Size: 2821 1165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 3954
Tth111I GACn'n_nGTC
Cuts at: 1783 1783
Size: 3986
XbaI T'CTAG_A
Cuts at: 1017 1017
Size: 3986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 546 831 1481 546
Size: 285 650 3051
Fragments arranged by size:
3051 650 285
XmaI C'CCGG_G
Cuts at: 569 1006 569
Size: 437 3549
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI