Restriction map for
pF1KB6345
(Circular) (Six-base) MAPSORT of: pF1KB6345.seq Check: 2743 from: 1 to: 3908
pF1KB6345 3908 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 946 946
Size: 3908
Acc65I G'GTAC_C
Cuts at: 924 924
Size: 3908
AclI AA'CG_TT
Cuts at: 3748 3748
Size: 3908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1733 2165 2912 3180 1733
Size: 432 747 268 2461
Fragments arranged by size:
2461 747 432 268
AflIII A'CryG_T
Cuts at: 1260 2364 3341 1260
Size: 1104 977 1827
Fragments arranged by size:
1827 1104 977
AgeI A'CCGG_T
Cuts at: 2296 2296
Size: 3908
AlwNI CAG_nnn'CTG
Cuts at: 285 1213 2780 285
Size: 928 1567 1413
Fragments arranged by size:
1567 1413 928
ApaI G_GGCC'C
Cuts at: 737 737
Size: 3908
ApaLI G'TGCA_C
Cuts at: 2678 2678
Size: 3908
ApoI r'AATT_y
Cuts at: 912 3261 3517 912
Size: 2349 256 1303
Fragments arranged by size:
2349 1303 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
AvaI C'yCGr_G
Cuts at: 446 928 446
Size: 482 3426
BamHI G'GATC_C
Cuts at: 933 933
Size: 3908
BanI G'GyrC_C
Cuts at: 397 432 924 1338 1586 1621 397
Size: 35 492 414 248 35 2684
Fragments arranged by size:
2684 492 414 248 35 35
BanII G_rGCy'C
Cuts at: 199 586 737 922 1952 199
Size: 387 151 185 1030 2155
Fragments arranged by size:
2155 1030 387 185 151
BbeI G_GCGC'C
Cuts at: 401 1342 1590 401
Size: 941 248 2719
Fragments arranged by size:
2719 941 248
BbsI GAAGACnn'nnnn_
Cuts at: 174 886 174
Size: 712 3196
BciVI GTATCCnnnnn_n'
Cuts at: 1806 2567 3908 1806
Size: 761 1341 1806
Fragments arranged by size:
1806 1341 761
BclI T'GATC_A
Cuts at: 1428 1428
Size: 3908
BglI GCCn_nnn'nGGC
Cuts at: 581 581
Size: 3908
BglII A'GATC_T
Cuts at: 350 1423 3460 350
Size: 1073 2037 798
Fragments arranged by size:
2037 1073 798
BlpI GC'TnA_GC
Cuts at: 1020 1020
Size: 3908
Bme1580I G_kGCm'C
Cuts at: 737 1533 1626 2682 737
Size: 796 93 1056 1963
Fragments arranged by size:
1963 1056 796 93
BmrI ACTGGGnnnn_n'
Cuts at: 726 861 1120 1201 1536 3680 726
Size: 135 259 81 335 2144 954
Fragments arranged by size:
2144 954 335 259 135 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 141 2275 141
Size: 2134 1774
Bpu10I CC'TnA_GC
Cuts at: 796 796
Size: 3908
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 190 1084 1963 2455 2753 124
Size: 66 894 879 492 298 1279
Fragments arranged by size:
1279 894 879 492 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 3612 3612
Size: 3908
BsaAI yAC'GTr
Cuts at: 378 833 1261 378
Size: 455 428 3025
Fragments arranged by size:
3025 455 428
BsaBI GATnn'nnATC
Cuts at: 6 1422 3459 6
Size: 1416 2037 455
Fragments arranged by size:
2037 1416 455
BsaHI Gr'CG_yC
Cuts at: 398 1339 1587 2289 3785 398
Size: 941 248 702 1496 521
Fragments arranged by size:
1496 941 702 521 248
BsaWI w'CCGG_w
Cuts at: 1097 1618 2296 2570 2717 3128 1097
Size: 521 678 274 147 411 1877
Fragments arranged by size:
1877 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 871 871
Size: 3908
BseYI C'CCAG_C
Cuts at: 386 588 663 1333 2091 2668 3164 386
Size: 202 75 670 758 577 496 1130
Fragments arranged by size:
1130 758 670 577 496 202 75
BsiEI CG_ry'CG
Cuts at: 104 1450 1496 2704 104
Size: 1346 46 1208 1308
Fragments arranged by size:
1346 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 127 922 1700 1890 2682 127
Size: 795 778 190 792 1353
Fragments arranged by size:
1353 795 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 127 199 586 737 922 1533 1626 1700 1890
Size: 72 387 151 185 611 93 74 190
Cuts at: 1890 1952 2682 127
Size: 62 730 1353
Fragments arranged by size:
1353 730 611 387 190 185 151 93
74 72 62
BspEI T'CCGG_A
Cuts at: 3128 3128
Size: 3908
BspHI T'CATG_A
Cuts at: 692 3084 692
Size: 2392 1516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 785 958 1172 1474 1855 785
Size: 173 214 302 381 2838
Fragments arranged by size:
2838 381 302 214 173
BsrBI CCG'CTC
Cuts at: 50 299 438 671 712 873 2200 2254 3738
Size: 249 139 233 41 161 1327 54 1484
Cuts at: 3738 3903 50
Size: 165 55
Fragments arranged by size:
1484 1327 249 233 165 161 139 55
54 41
BsrDI GCAATG_nn'
Cuts at: 401 1820 401
Size: 1419 2489
BsrFI r'CCGG_y
Cuts at: 1906 2087 2296 1906
Size: 181 209 3518
Fragments arranged by size:
3518 209 181
BssSI C'ACGA_G
Cuts at: 2179 2537 2179
Size: 358 3550
BstAPI GCAn_nnn'nTGC
Cuts at: 1285 1285
Size: 3908
BstBI TT'CG_AA
Cuts at: 2269 2269
Size: 3908
BstXI CCAn_nnnn'nTGG
Cuts at: 327 507 579 327
Size: 180 72 3656
Fragments arranged by size:
3656 180 72
BstYI r'GATC_y
Cuts at: 1 350 933 1400 1423 1758 2004 3016 3102
Size: 349 583 467 23 335 246 1012 86
Cuts at: 3102 3114 3460 1
Size: 12 346 449
Fragments arranged by size:
1012 583 467 449 349 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 128 500 884 128
Size: 372 384 3152
Fragments arranged by size:
3152 384 372
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1858 2039 2202 1858
Size: 181 163 3564
Fragments arranged by size:
3564 181 163
BtsI GCAGTG_nn'
Cuts at: 1151 1151
Size: 3908
DraI TTT'AAA
Cuts at: 907 907
Size: 3908
DrdI GACnn_nn'nnGTC
Cuts at: 1614 2472 1614
Size: 858 3050
EaeI y'GGCC_r
Cuts at: 164 211 260 303 458 815 1493 1667 2058
Size: 47 49 43 155 357 678 174 391
Cuts at: 2058 2085 3430 164
Size: 27 1345 642
Fragments arranged by size:
1345 678 642 391 357 174 155 49
47 43 27
EagI C'GGCC_G
Cuts at: 1493 1493
Size: 3908
EarI CTCTTCn'nnn_
Cuts at: 787 863 1931 2141 787
Size: 76 1068 210 2554
Fragments arranged by size:
2554 1068 210 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2116 2426 2572 3718 2116
Size: 310 146 1146 2306
Fragments arranged by size:
2306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 920 920
Size: 3908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1733 2165 2912 3180 1733
Size: 432 747 268 2461
Fragments arranged by size:
2461 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 282 289 733 1047 282
Size: 7 444 314 3143
Fragments arranged by size:
3143 444 314 7
EcoRI G'AATT_C
Cuts at: 912 912
Size: 3908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1927 1927
Size: 3908
FspI TGC'GCA
Cuts at: 1689 1689
Size: 3908
HaeII r_GCGC'y
Cuts at: 223 401 1342 1590 2612 3591 223
Size: 178 941 248 1022 979 540
Fragments arranged by size:
1022 979 941 540 248 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1750 1780 1782 1812 1750
Size: 30 2 30 3846
Fragments arranged by size:
3846 30 30 2
HincII GTy'rAC
Cuts at: 465 947 465
Size: 482 3426
KasI G'GCGC_C
Cuts at: 397 1338 1586 397
Size: 941 248 2719
Fragments arranged by size:
2719 941 248
KpnI G_GTAC'C
Cuts at: 928 928
Size: 3908
MluI A'CGCG_T
Cuts at: 3341 3341
Size: 3908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 612 2579 2763 612
Size: 1967 184 1757
Fragments arranged by size:
1967 1757 184
MscI TGG'CCA
Cuts at: 166 262 817 1669 166
Size: 96 555 852 2405
Fragments arranged by size:
2405 852 555 96
MslI CAynn'nnrTG
Cuts at: 505 1897 2024 505
Size: 1392 127 2389
Fragments arranged by size:
2389 1392 127
MspA1I CmG'CkG
Cuts at: 390 531 864 1019 1333 1693 2706 2951 390
Size: 141 333 155 314 360 1013 245 1347
Fragments arranged by size:
1347 1013 360 333 314 245 155 141
NaeI GCC'GGC
Cuts at: 2089 2089
Size: 3908
NarI GG'CG_CC
Cuts at: 398 1339 1587 398
Size: 941 248 2719
Fragments arranged by size:
2719 941 248
NcoI C'CATG_G
Cuts at: 500 884 500
Size: 384 3524
NgoMIV G'CCGG_C
Cuts at: 2087 2087
Size: 3908
NspI r_CATG'y
Cuts at: 961 1461 2368 961
Size: 500 907 2501
Fragments arranged by size:
2501 907 500
PasI CC'CwG_GG
Cuts at: 285 618 285
Size: 333 3575
PciI A'CATG_T
Cuts at: 2364 2364
Size: 3908
PflMI CCAn_nnn'nTGG
Cuts at: 285 393 501 285
Size: 108 108 3692
Fragments arranged by size:
3692 108 108
PmeI GTTT'AAAC
Cuts at: 907 907
Size: 3908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3047 3079 3047
Size: 32 3876
PpuMI rG'GwC_Cy
Cuts at: 289 289
Size: 3908
PspOMI G'GGCC_C
Cuts at: 733 733
Size: 3908
PstI C_TGCA'G
Cuts at: 239 955 1640 239
Size: 716 685 2507
Fragments arranged by size:
2507 716 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3908
PvuII CAG'CTG
Cuts at: 390 531 1333 1693 390
Size: 141 802 360 2605
Fragments arranged by size:
2605 802 360 141
RsrII CG'GwC_CG
Cuts at: 2103 2103
Size: 3908
SacI G_AGCT'C
Cuts at: 922 922
Size: 3908
SalI G'TCGA_C
Cuts at: 945 945
Size: 3908
SanDI GG'GwC_CC
Cuts at: 289 289
Size: 3908
SapI GCTCTTCn'nnn_
Cuts at: 787 863 1931 2141 787
Size: 76 1068 210 2554
Fragments arranged by size:
2554 1068 210 76
SbfI CC_TGCA'GG
Cuts at: 955 955
Size: 3908
ScaI AGT'ACT
Cuts at: 3425 3425
Size: 3908
SfcI C'TryA_G
Cuts at: 33 235 951 1636 2629 2820 33
Size: 202 716 685 993 191 1121
Fragments arranged by size:
1121 993 716 685 202 191
SfoI GGC'GCC
Cuts at: 399 1340 1588 399
Size: 941 248 2719
Fragments arranged by size:
2719 941 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
SmaI CCC'GGG
Cuts at: 448 930 448
Size: 482 3426
SmlI C'TyrA_G
Cuts at: 139 205 1063 1978 2470 2732 139
Size: 66 858 915 492 262 1315
Fragments arranged by size:
1315 915 858 492 262 66
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3908
SphI G_CATG'C
Cuts at: 961 1461 961
Size: 500 3408
StyI C'CwwG_G
Cuts at: 500 884 1042 1396 500
Size: 384 158 354 3012
Fragments arranged by size:
3012 384 354 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1491 1624 2083 2292 1491
Size: 133 459 209 3107
Fragments arranged by size:
3107 459 209 133
TatI w'GTAC_w
Cuts at: 3423 3423
Size: 3908
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 3876
Tth111I GACn'n_nGTC
Cuts at: 1705 1705
Size: 3908
XbaI T'CTAG_A
Cuts at: 939 939
Size: 3908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 1403 270
Size: 1133 2775
XmaI C'CCGG_G
Cuts at: 446 928 446
Size: 482 3426
XmnI GAAnn'nnTTC
Cuts at: 700 700
Size: 3908
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI