Restriction map for
pF1KB6348
(Circular) (Six-base) MAPSORT of: pF1KB6348.seq Check: 5341 from: 1 to: 3992
pF1KB6348 3992 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 130 1030 130
Size: 900 3092
Acc65I G'GTAC_C
Cuts at: 1008 1008
Size: 3992
AclI AA'CG_TT
Cuts at: 3832 3832
Size: 3992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 908 1817 2249 2996 3264 176
Size: 732 909 432 747 268 904
Fragments arranged by size:
909 904 747 732 432 268
AflIII A'CryG_T
Cuts at: 1344 2448 3425 1344
Size: 1104 977 1911
Fragments arranged by size:
1911 1104 977
AgeI A'CCGG_T
Cuts at: 2380 2380
Size: 3992
AlwNI CAG_nnn'CTG
Cuts at: 700 1297 2864 700
Size: 597 1567 1828
Fragments arranged by size:
1828 1567 597
ApaLI G'TGCA_C
Cuts at: 765 2762 765
Size: 1997 1995
ApoI r'AATT_y
Cuts at: 352 996 3345 3601 352
Size: 644 2349 256 743
Fragments arranged by size:
2349 743 644 256
AseI AT'TA_AT
Cuts at: 20 418 640 20
Size: 398 222 3372
Fragments arranged by size:
3372 398 222
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
AvaI C'yCGr_G
Cuts at: 1012 1012
Size: 3992
BamHI G'GATC_C
Cuts at: 1017 1017
Size: 3992
BanI G'GyrC_C
Cuts at: 1008 1422 1670 1705 1008
Size: 414 248 35 3295
Fragments arranged by size:
3295 414 248 35
BanII G_rGCy'C
Cuts at: 1006 2036 1006
Size: 1030 2962
BbeI G_GCGC'C
Cuts at: 1426 1674 1426
Size: 248 3744
BbsI GAAGACnn'nnnn_
Cuts at: 175 806 175
Size: 631 3361
BciVI GTATCCnnnnn_n'
Cuts at: 1890 2651 3992 1890
Size: 761 1341 1890
Fragments arranged by size:
1890 1341 761
BclI T'GATC_A
Cuts at: 1512 1512
Size: 3992
BglII A'GATC_T
Cuts at: 1507 3544 1507
Size: 2037 1955
BlpI GC'TnA_GC
Cuts at: 1104 1104
Size: 3992
Bme1580I G_kGCm'C
Cuts at: 769 1617 1710 2766 769
Size: 848 93 1056 1995
Fragments arranged by size:
1995 1056 848 93
BmgBI CAC'GTC
Cuts at: 186 186
Size: 3992
BmrI ACTGGGnnnn_n'
Cuts at: 1204 1285 1620 3764 1204
Size: 81 335 2144 1432
Fragments arranged by size:
2144 1432 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 3992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 141
Size: 3992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 2047 2539 2837 1168
Size: 879 492 298 2323
Fragments arranged by size:
2323 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3696 3696
Size: 3992
BsaAI yAC'GTr
Cuts at: 1345 1345
Size: 3992
BsaBI GATnn'nnATC
Cuts at: 6 500 1506 3543 6
Size: 494 1006 2037 455
Fragments arranged by size:
2037 1006 494 455
BsaHI Gr'CG_yC
Cuts at: 386 1423 1671 2373 3869 386
Size: 1037 248 702 1496 509
Fragments arranged by size:
1496 1037 702 509 248
BsaWI w'CCGG_w
Cuts at: 1181 1702 2380 2654 2801 3212 1181
Size: 521 678 274 147 411 1961
Fragments arranged by size:
1961 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 183 213 183
Size: 30 3962
BseYI C'CCAG_C
Cuts at: 279 1417 2175 2752 3248 279
Size: 1138 758 577 496 1023
Fragments arranged by size:
1138 1023 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 415 194
Size: 221 3771
BsiEI CG_ry'CG
Cuts at: 104 1534 1580 2788 104
Size: 1430 46 1208 1308
Fragments arranged by size:
1430 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 769 1006 1784 1974 2766 769
Size: 237 778 190 792 1995
Fragments arranged by size:
1995 792 778 237 190
Bsp1286I G_dGCh'C
Cuts at: 769 1006 1617 1710 1784 1974 2036 2766 769
Size: 237 611 93 74 190 62 730 1995
Fragments arranged by size:
1995 730 611 237 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3212 3212
Size: 3992
BspHI T'CATG_A
Cuts at: 3168 3168
Size: 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 624 1042 1256 1558 1939 624
Size: 418 214 302 381 2677
Fragments arranged by size:
2677 418 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2284 2338 3822 3987 50
Size: 2234 54 1484 165 55
Fragments arranged by size:
2234 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1904 1904
Size: 3992
BsrFI r'CCGG_y
Cuts at: 1990 2171 2380 1990
Size: 181 209 3602
Fragments arranged by size:
3602 209 181
BsrGI T'GTAC_A
Cuts at: 840 840
Size: 3992
BssSI C'ACGA_G
Cuts at: 2263 2621 2263
Size: 358 3634
BstAPI GCAn_nnn'nTGC
Cuts at: 1369 1369
Size: 3992
BstBI TT'CG_AA
Cuts at: 2353 2353
Size: 3992
BstYI r'GATC_y
Cuts at: 1 1017 1484 1507 1842 2088 3100 3186 3198
Size: 1016 467 23 335 246 1012 86 12
Cuts at: 3198 3544 1
Size: 346 449
Fragments arranged by size:
1016 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 570 570
Size: 3992
BtgI C'CryG_G
Cuts at: 826 826
Size: 3992
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1942 2123 2286 1942
Size: 181 163 3648
Fragments arranged by size:
3648 181 163
BtsI GCAGTG_nn'
Cuts at: 767 1235 767
Size: 468 3524
DraI TTT'AAA
Cuts at: 735 991 735
Size: 256 3736
DrdI GACnn_nn'nnGTC
Cuts at: 1698 2556 1698
Size: 858 3134
EaeI y'GGCC_r
Cuts at: 1577 1751 2142 2169 3514 1577
Size: 174 391 27 1345 2055
Fragments arranged by size:
2055 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1577 1577
Size: 3992
EarI CTCTTCn'nnn_
Cuts at: 158 347 583 962 2015 2225 158
Size: 189 236 379 1053 210 1925
Fragments arranged by size:
1925 1053 379 236 210 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 627 2200 2510 2656 3802 627
Size: 1573 310 146 1146 817
Fragments arranged by size:
1573 1146 817 310 146
EcoICRI GAG'CTC
Cuts at: 1004 1004
Size: 3992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 176 908 1817 2249 2996 3264 141
Size: 35 732 909 432 747 268 869
Fragments arranged by size:
909 869 747 732 432 268 35
EcoO109I rG'GnC_Cy
Cuts at: 1131 1131
Size: 3992
EcoRI G'AATT_C
Cuts at: 996 996
Size: 3992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2011 2011
Size: 3992
FspI TGC'GCA
Cuts at: 1773 1773
Size: 3992
HaeII r_GCGC'y
Cuts at: 1426 1674 2696 3675 1426
Size: 248 1022 979 1743
Fragments arranged by size:
1743 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 553 585 855 887 1834 1864 1866 1896 553
Size: 32 270 32 947 30 2 30 2649
Fragments arranged by size:
2649 947 270 32 32 30 30 2
HincII GTy'rAC
Cuts at: 293 750 1031 293
Size: 457 281 3254
Fragments arranged by size:
3254 457 281
KasI G'GCGC_C
Cuts at: 1422 1670 1422
Size: 248 3744
KpnI G_GTAC'C
Cuts at: 1012 1012
Size: 3992
MluI A'CGCG_T
Cuts at: 3425 3425
Size: 3992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2663 2847 2663
Size: 184 3808
MscI TGG'CCA
Cuts at: 1753 1753
Size: 3992
MslI CAynn'nnrTG
Cuts at: 420 1981 2108 420
Size: 1561 127 2304
Fragments arranged by size:
2304 1561 127
MspA1I CmG'CkG
Cuts at: 225 1103 1417 1777 2790 3035 225
Size: 878 314 360 1013 245 1182
Fragments arranged by size:
1182 1013 878 360 314 245
NaeI GCC'GGC
Cuts at: 2173 2173
Size: 3992
NarI GG'CG_CC
Cuts at: 1423 1671 1423
Size: 248 3744
NcoI C'CATG_G
Cuts at: 826 826
Size: 3992
NgoMIV G'CCGG_C
Cuts at: 2171 2171
Size: 3992
NspI r_CATG'y
Cuts at: 129 310 1045 1545 2452 129
Size: 181 735 500 907 1669
Fragments arranged by size:
1669 907 735 500 181
PciI A'CATG_T
Cuts at: 2448 2448
Size: 3992
PmeI GTTT'AAAC
Cuts at: 991 991
Size: 3992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 3131
Size: 32 3960
PstI C_TGCA'G
Cuts at: 705 1039 1724 705
Size: 334 685 2973
Fragments arranged by size:
2973 685 334
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3992
PvuII CAG'CTG
Cuts at: 225 1417 1777 225
Size: 1192 360 2440
Fragments arranged by size:
2440 1192 360
RsrII CG'GwC_CG
Cuts at: 2187 2187
Size: 3992
SacI G_AGCT'C
Cuts at: 1006 1006
Size: 3992
SalI G'TCGA_C
Cuts at: 1029 1029
Size: 3992
SapI GCTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
SbfI CC_TGCA'GG
Cuts at: 1039 1039
Size: 3992
ScaI AGT'ACT
Cuts at: 707 3509 707
Size: 2802 1190
SfcI C'TryA_G
Cuts at: 33 701 1035 1720 2713 2904 33
Size: 668 334 685 993 191 1121
Fragments arranged by size:
1121 993 685 668 334 191
SfoI GGC'GCC
Cuts at: 1424 1672 1424
Size: 248 3744
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
SmaI CCC'GGG
Cuts at: 1014 1014
Size: 3992
SmlI C'TyrA_G
Cuts at: 1147 2062 2554 2816 1147
Size: 915 492 262 2323
Fragments arranged by size:
2323 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3992
SphI G_CATG'C
Cuts at: 310 1045 1545 310
Size: 735 500 2757
Fragments arranged by size:
2757 735 500
SspI AAT'ATT
Cuts at: 437 437
Size: 3992
StyI C'CwwG_G
Cuts at: 359 826 1126 1480 359
Size: 467 300 354 2871
Fragments arranged by size:
2871 467 354 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1575 1708 2167 2376 1575
Size: 133 459 209 3191
Fragments arranged by size:
3191 459 209 133
TatI w'GTAC_w
Cuts at: 705 840 948 3507 705
Size: 135 108 2559 1190
Fragments arranged by size:
2559 1190 135 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 3960
Tth111I GACn'n_nGTC
Cuts at: 1789 1789
Size: 3992
XbaI T'CTAG_A
Cuts at: 1023 1023
Size: 3992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1487 1487
Size: 3992
XmaI C'CCGG_G
Cuts at: 1012 1012
Size: 3992
XmnI GAAnn'nnTTC
Cuts at: 115 115
Size: 3992
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI