FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KB6354, 295 aa 1>>>pF1KB6354 295 - 295 aa - 295 aa Library: human.CCDS.faa 18511270 residues in 32554 sequences Statistics: Expectation_n fit: rho(ln(x))= 6.0271+/-0.000843; mu= 11.8543+/- 0.050 mean_var=79.0204+/-15.689, 0's: 0 Z-trim(108.7): 29 B-trim: 212 in 1/50 Lambda= 0.144279 statistics sampled from 10377 (10406) to 10377 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.699), E-opt: 0.2 (0.32), width: 16 Scan time: 2.510 The best scores are: opt bits E(32554) CCDS34082.1 SFRP2 gene_id:6423|Hs108|chr4 ( 295) 2065 439.1 1.8e-123 CCDS7472.1 SFRP5 gene_id:6425|Hs108|chr10 ( 317) 826 181.2 8.5e-46 CCDS34886.1 SFRP1 gene_id:6422|Hs108|chr8 ( 314) 811 178.1 7.3e-45 CCDS7192.1 FZD8 gene_id:8325|Hs108|chr10 ( 694) 395 91.7 1.7e-18 CCDS5548.1 FZD9 gene_id:8326|Hs108|chr7 ( 591) 382 88.9 9.6e-18 CCDS9267.1 FZD10 gene_id:11211|Hs108|chr12 ( 581) 375 87.5 2.6e-17 CCDS5620.1 FZD1 gene_id:8321|Hs108|chr7 ( 647) 374 87.3 3.3e-17 CCDS8279.1 FZD4 gene_id:8322|Hs108|chr11 ( 537) 370 86.4 5e-17 CCDS2351.1 FZD7 gene_id:8324|Hs108|chr2 ( 574) 365 85.4 1.1e-16 CCDS33366.1 FZD5 gene_id:7855|Hs108|chr2 ( 585) 362 84.8 1.7e-16 CCDS11484.1 FZD2 gene_id:2535|Hs108|chr17 ( 565) 356 83.5 3.9e-16 CCDS2286.1 FRZB gene_id:2487|Hs108|chr2 ( 325) 337 79.5 3.8e-15 CCDS6069.1 FZD3 gene_id:7976|Hs108|chr8 ( 666) 311 74.2 3e-13 CCDS63958.1 CORIN gene_id:10699|Hs108|chr4 ( 734) 302 72.3 1.2e-12 CCDS5453.1 SFRP4 gene_id:6424|Hs108|chr7 ( 346) 297 71.1 1.3e-12 CCDS75122.1 CORIN gene_id:10699|Hs108|chr4 ( 938) 302 72.4 1.5e-12 CCDS3477.1 CORIN gene_id:10699|Hs108|chr4 (1042) 302 72.4 1.6e-12 CCDS6298.1 FZD6 gene_id:8323|Hs108|chr8 ( 706) 291 70.0 5.7e-12 >>CCDS34082.1 SFRP2 gene_id:6423|Hs108|chr4 (295 aa) initn: 2065 init1: 2065 opt: 2065 Z-score: 2330.0 bits: 439.1 E(32554): 1.8e-123 Smith-Waterman score: 2065; 100.0% identity (100.0% similar) in 295 aa overlap (1-295:1-295) 10 20 30 40 50 60 pF1KB6 MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQNMRL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS34 MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQNMRL 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB6 PNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS34 PNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQ 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB6 VKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKND :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS34 VKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKND 130 140 150 160 170 180 190 200 210 220 230 240 pF1KB6 DDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS34 DDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLK 190 200 210 220 230 240 250 260 270 280 290 pF1KB6 DSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC ::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS34 DSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC 250 260 270 280 290 >>CCDS7472.1 SFRP5 gene_id:6425|Hs108|chr10 (317 aa) initn: 573 init1: 322 opt: 826 Z-score: 935.7 bits: 181.2 E(32554): 8.5e-46 Smith-Waterman score: 826; 45.1% identity (71.8% similar) in 266 aa overlap (34-295:46-303) 10 20 30 40 50 60 pF1KB6 GPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRS-NCKPIPANLQLCHGIEYQNMRLPN ::.. .: :::.: ::: . :. ::::: CCDS74 ALLLGALHWAPARCEEYDYYGWQAEPLHGRSYSKPPQCLDIPADLPLCHTVGYKRMRLPN 20 30 40 50 60 70 70 80 90 100 110 120 pF1KB6 LLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVK :: ::.. :: .::..:.::. :.:: ::. :::::::::: ::. : ::.::: :. CCDS74 LLEHESLAEVKQQASSWLPLLAKRCHSDTQVFLCSLFAPVC---LDRPIYPCRSLCEAVR 80 90 100 110 120 130 130 140 150 160 170 pF1KB6 DRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAP---KVCEACKNKN :::.: :.:::::.::.: .:: :::::: . . :: ::: :: :.: :. .. CCDS74 AGCAPLMEAYGFPWPEMLHCHKFPLDNDLCIAV-QFGHL-PAT--APPVTKICAQCEMEH 140 150 160 170 180 180 190 200 210 220 230 pF1KB6 DDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWL . :. .:: .:..::..:...::: : : :.: :.: . : . ....: :. :: . CCDS74 SADG-LMEQMCSSDFVVKMRIKEIKIENGDRKLIGAQKKKKLLKPGPLKRKDTKRLVLHM 190 200 210 220 230 240 240 250 260 270 280 290 pF1KB6 KDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC :.. : : ..... . .::::.: :.:.. .: ::.: ..:.: . . . : CCDS74 KNGAGCPCPQLDSLAGSFLVMGRKVDGQLLLMAVYRWDKKNKEMKFAVKFMFSYPCSLYY 250 260 270 280 290 300 CCDS74 PFFYGAAEPH 310 >>CCDS34886.1 SFRP1 gene_id:6422|Hs108|chr8 (314 aa) initn: 616 init1: 319 opt: 811 Z-score: 918.9 bits: 178.1 E(32554): 7.3e-45 Smith-Waterman score: 811; 42.6% identity (72.6% similar) in 263 aa overlap (33-295:51-306) 10 20 30 40 50 60 pF1KB6 QGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQNMRLPN : : .: :::.:.:::.. :..: ::: CCDS34 LGAALLAVGSASEYDYVSFQSDIGPYQSGRFYTKPPQCVDIPADLRLCHNVGYKKMVLPN 30 40 50 60 70 80 70 80 90 100 110 120 pF1KB6 LLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVK :: :::: :: .::..:.::. :.:: :. :::::::::::: . : ::. :: :. CCDS34 LLEHETMAEVKQQASSWVPLLNKNCHAGTQVFLCSLFAPVCLD---RPIYPCRWLCEAVR 90 100 110 120 130 130 140 150 160 170 180 pF1KB6 DRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDD : : :::. ::: ::.::.::.::. .:.:: .. . . .. :: : :. .. CCDS34 DSCEPVMQFFGFYWPEMLKCDKFPE-GDVCIAMTPPNATEASKPQGTTVCPPCDNELKSE 140 150 160 170 180 190 190 200 210 220 230 240 pF1KB6 NDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLKDS :.: :: ..:::..:.::. : : ::. :.: ::. ....:::: ::.::.. CCDS34 A-IIEHLCASEFALRMKIKEVKKENGDKKIV--PKKKKPLKLGPIKKKDLKKLVLYLKNG 200 210 220 230 240 250 250 260 270 280 290 pF1KB6 LQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC .: :....... .:.::.: .. ..:....:.: ..::: . ..... .: CCDS34 ADCPCHQLDNLSHHFLIMGRKVKSQYLLTAIHKWDKKNKEFKNFMKKMKNHECPTFQSVF 260 270 280 290 300 310 CCDS34 K >>CCDS7192.1 FZD8 gene_id:8325|Hs108|chr10 (694 aa) initn: 366 init1: 366 opt: 395 Z-score: 445.6 bits: 91.7 E(32554): 1.7e-18 Smith-Waterman score: 395; 39.7% identity (64.4% similar) in 146 aa overlap (29-170:24-165) 10 20 30 40 50 60 pF1KB6 MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQNMRL : : :. :. : .. ::.:: :. . CCDS71 MEWGYLLEVTSLLAALALLQRSSGAAAASAKELACQEI--TVPLCKGIGYNYTYM 10 20 30 40 50 70 80 90 100 110 pF1KB6 PNLLGHETMKEV-LEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCV :: ..:.:. :. :: : ::: :: :: : ::::...:.::.: . . ::.:.: CCDS71 PNQFNHDTQDEAGLEVHQFW-PLVEIQCSPDLKFFLCSMYTPICLEDYKKPLPPCRSVCE 60 70 80 90 100 110 120 130 140 150 160 170 pF1KB6 QVKDRCAPVMSAFGFPWPDMLECDRFPQDND---LCIPLASSDHLLPATEEAPKVCEACK ..: :::.: .:: ::: ..:::.:.... ::. .: : :. :. CCDS71 RAKAGCAPLMRQYGFAWPDRMRCDRLPEQGNPDTLCMDYNRTD-LTTAAPSPPRRLPPPP 120 130 140 150 160 170 180 190 200 210 220 230 pF1KB6 NKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSV CCDS71 PGEQPPSGSGHGRPPGARPPHRGGGRGGGGGDAAAPPARGGGGGGKARPPGGGAAPCEPG 180 190 200 210 220 230 >>CCDS5548.1 FZD9 gene_id:8326|Hs108|chr7 (591 aa) initn: 367 init1: 367 opt: 382 Z-score: 432.0 bits: 88.9 E(32554): 9.6e-18 Smith-Waterman score: 382; 37.4% identity (62.0% similar) in 163 aa overlap (6-164:8-164) 10 20 30 40 50 pF1KB6 MLQGPGSLLLL-FLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQN :.::: .::. :.: . : .:. . . :. . .. .:.:: :. CCDS55 MAVAPLRGALLLWQLLAAG---GAALEIGRF-DPERGRGAAPCQAV--EIPMCRGIGYNL 10 20 30 40 50 60 70 80 90 100 110 pF1KB6 MRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSL :.:::::: .. :. . . . :::. :: . :::::.::.: :... : :. . CCDS55 TRMPNLLGHTSQGEAAAELAEFAPLVQYGCHSHLRFFLCSLYAPMCTDQVSTPIPACRPM 60 70 80 90 100 110 120 130 140 150 160 170 pF1KB6 CVQVKDRCAPVMSAFGFPWPDMLECDRFPQDND---LCIPLASSDHLLPATEEAPKVCEA : :.. ::::.: :.: ::: :.: :.: :: ::. . :: CCDS55 CEQARLRCAPIMEQFNFGWPDSLDCARLPTRNDPHALCMEAPENATAGPAEPHKGLGMLP 120 130 140 150 160 170 180 190 200 210 220 230 pF1KB6 CKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKK CCDS55 VAPRPARPPGDLGPGAGGSGTCENPEKFQYVEKSRSCAPRCGPGVEVFWSRRDKDFALVW 180 190 200 210 220 230 >>CCDS9267.1 FZD10 gene_id:11211|Hs108|chr12 (581 aa) initn: 382 init1: 358 opt: 375 Z-score: 424.3 bits: 87.5 E(32554): 2.6e-17 Smith-Waterman score: 376; 35.8% identity (60.6% similar) in 165 aa overlap (2-159:1-156) 10 20 30 40 50 pF1KB6 MLQGPGSLLLLFLA--SHCCLGSARGLFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQNM .: :: : : : . : :. . :. ..:.:: .. .:. : :. CCDS92 MQRPGPRLWLVLQVMGSCAAISSMDMERPGD-------GKCQPI--EIPMCKDIGYNMT 10 20 30 40 50 60 70 80 90 100 110 pF1KB6 RLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLC :.:::.:::...:. : . ::: :: . :::::.::.: .... : :. .: CCDS92 RMPNLMGHENQREAAIQLHEFAPLVEYGCHGHLRFFLCSLYAPMCTEQVSTPIPACRVMC 60 70 80 90 100 110 120 130 140 150 160 170 pF1KB6 VQVKDRCAPVMSAFGFPWPDMLECDRFPQDND---LCI--PLASSDHLLPATEEAPKVCE :.. .:.:.: :.: ::: :.: ..:. :: ::. : .:: CCDS92 EQARLKCSPIMEQFNFKWPDSLDCRKLPNKNDPNYLCMEAPNNGSDEPTRGSGLFPPLFR 120 130 140 150 160 170 180 190 200 210 220 230 pF1KB6 ACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLK CCDS92 PQRPHSAQEHPLKDGGPGRGGCDNPGKFHHVEKSASCAPLCTPGVDVYWSREDKRFAVVW 180 190 200 210 220 230 >>CCDS5620.1 FZD1 gene_id:8321|Hs108|chr7 (647 aa) initn: 338 init1: 182 opt: 374 Z-score: 422.4 bits: 87.3 E(32554): 3.3e-17 Smith-Waterman score: 374; 38.0% identity (65.5% similar) in 142 aa overlap (31-170:111-245) 10 20 30 40 50 60 pF1KB6 MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQNMRL :: .: :.:: .. :: : :.. . CCDS56 QGPGPGQQPPPPPQQQQSGQQYNGERGISVPDHGY----CQPI--SIPLCTDIAYNQTIM 90 100 110 120 130 70 80 90 100 110 120 pF1KB6 PNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQ :::::: ..... .. . ::: :: . : ::::..:::: :.... ::.::: . CCDS56 PNLLGHTNQEDAGLEVHQFYPLVKVQCSAELKFFLCSMYAPVCTV-LEQALPPCRSLCER 140 150 160 170 180 190 130 140 150 160 170 pF1KB6 VKDRCAPVMSAFGFPWPDMLECDRFPQDN--DLCIPLASSDHLLPATEEAPKVCEACKNK ... : .:. ::: ::: :.:..:: . .::. .::. :. :. CCDS56 ARQGCEALMNKFGFQWPDTLKCEKFPVHGAGELCVGQNTSDKGTPTPSLLPEFWTSNPQH 200 210 220 230 240 250 180 190 200 210 220 230 pF1KB6 NDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLW CCDS56 GGGGHRGGFPGGAGASERGKFSCPRALKVPSYLNYHFLGEKDCGAPCEPTKVYGLMYFGP 260 270 280 290 300 310 >>CCDS8279.1 FZD4 gene_id:8322|Hs108|chr11 (537 aa) initn: 349 init1: 349 opt: 370 Z-score: 419.2 bits: 86.4 E(32554): 5e-17 Smith-Waterman score: 393; 33.2% identity (63.6% similar) in 187 aa overlap (4-186:16-192) 10 20 30 40 pF1KB6 MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPANLQ : : : :.: :: ::: :: : .: : :: .. CCDS82 MAWRGAGPSVPGAPGGVGLSLGLLLQLLLLLGPARG---FG--DEEERR--CDPI--RIS 10 20 30 40 50 50 60 70 80 90 100 pF1KB6 LCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLD .:... :. ..:::.::: . .. : .. ::.. : . . ::::...:.: . .. CCDS82 MCQNLGYNVTKMPNLVGHELQTDAELQLTTFTPLIQYGCSSQLQFFLCSVYVPMCTEKIN 60 70 80 90 100 110 110 120 130 140 150 160 pF1KB6 ETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDND---LCIPLASSDHLLPAT : :: ..:..:: :: ::.. ::: ::. :.:..:: .:: .:. . .:. .: CCDS82 IPIGPCGGMCLSVKRRCEPVLKEFGFAWPESLNCSKFPPQNDHNHMCME-GPGDEEVPLP 120 130 140 150 160 170 170 180 190 200 210 220 pF1KB6 EEAP-KVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKL ...: . : :.. . .... . CCDS82 HKTPIQPGEECHSVGTNSDQYIWVKRSLNCVLKCGYDAGLYSRSAKEFTDIWMAVWASLC 180 190 200 210 220 230 >>CCDS2351.1 FZD7 gene_id:8324|Hs108|chr2 (574 aa) initn: 345 init1: 192 opt: 365 Z-score: 413.1 bits: 85.4 E(32554): 1.1e-16 Smith-Waterman score: 365; 39.7% identity (67.2% similar) in 131 aa overlap (31-159:44-167) 10 20 30 40 50 60 pF1KB6 MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQNMRL :: .. :.:: .. :: : :.. : CCDS23 GLCALVLALLGALSAGAGAQPYHGEKGISVPDHGF----CQPI--SIPLCTDIAYNQTIL 20 30 40 50 60 70 80 90 100 110 120 pF1KB6 PNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQ :::::: ..... .. . ::: :: :. . ::::..:::: ::..: ::.::: . CCDS23 PNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTV-LDQAIPPCRSLCER 70 80 90 100 110 120 130 140 150 160 170 pF1KB6 VKDRCAPVMSAFGFPWPDMLECDRFPQDN--DLCIPLASSDHLLPATEEAPKVCEACKNK ... : .:. ::: ::. :.:. :: . ..:. .:: CCDS23 ARQGCEALMNKFGFQWPERLRCENFPVHGAGEICVGQNTSDGSGGPGGGPTAYPTAPYLP 130 140 150 160 170 180 180 190 200 210 220 230 pF1KB6 NDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLW CCDS23 DLPFTALPPGASDGRGRPAFPFSCPRQLKVPPYLGYRFLGERDCGAPCEPGRANGLMYFK 190 200 210 220 230 240 >>CCDS33366.1 FZD5 gene_id:7855|Hs108|chr2 (585 aa) initn: 372 init1: 342 opt: 362 Z-score: 409.6 bits: 84.8 E(32554): 1.7e-16 Smith-Waterman score: 374; 36.8% identity (59.6% similar) in 171 aa overlap (5-170:9-161) 10 20 30 40 50 pF1KB6 MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQ : :::::.::. : :. . : :. : .. .:.:: :. CCDS33 MARPDPSAPPSLLLLLLAQ-----------LVGRAAAASKAPVCQEI--TVPMCRGIGYN 10 20 30 40 60 70 80 90 100 110 pF1KB6 NMRLPNLLGHETMKEV-LEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCH ..:: ..:.:. :. :: : ::: :: :: . ::::...:.:: : . . ::. CCDS33 LTHMPNQFNHDTQDEAGLEVHQFW-PLVEIQCSPDLRFFLCSMYTPICLPDYHKPLPPCR 50 60 70 80 90 100 120 130 140 150 160 170 pF1KB6 SLCVQVKDRCAPVMSAFGFPWPDMLECDRFP---QDND-LCIPLASSDHLLPATEEAPKV :.: ..: :.:.: .:: ::. . :::.: .: . ::. :. :: :. CCDS33 SVCERAKAGCSPLMRQYGFAWPERMSCDRLPVLGRDAEVLCMDYNRSE----ATTAPPRP 110 120 130 140 150 160 180 190 200 210 220 230 pF1KB6 CEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERD CCDS33 FPAKPTLPGPPGAPASGGECPAGGPFVCKCREPFVPILKESHPLYNKVRTGQVPNCAVPC 170 180 190 200 210 220 295 residues in 1 query sequences 18511270 residues in 32554 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Fri Nov 4 20:36:25 2016 done: Fri Nov 4 20:36:25 2016 Total Scan time: 2.510 Total Display time: 0.010 Function used was FASTA [36.3.4 Apr, 2011]