Restriction map for
pF1KB6354
(Circular) (Six-base) MAPSORT of: pF1KB6354.seq Check: 4998 from: 1 to: 3998
pF1KB6354 3998 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 456 456
Size: 3998
AccI GT'mk_AC
Cuts at: 1036 1036
Size: 3998
Acc65I G'GTAC_C
Cuts at: 1014 1014
Size: 3998
AclI AA'CG_TT
Cuts at: 3838 3838
Size: 3998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 1823 2255 3002 3270 823
Size: 1000 432 747 268 1551
Fragments arranged by size:
1551 1000 747 432 268
AflIII A'CryG_T
Cuts at: 1350 2454 3431 1350
Size: 1104 977 1917
Fragments arranged by size:
1917 1104 977
AgeI A'CCGG_T
Cuts at: 2386 2386
Size: 3998
AleI CACnn'nnGTG
Cuts at: 922 922
Size: 3998
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 772 804 772
Size: 32 3966
AlwNI CAG_nnn'CTG
Cuts at: 1303 2870 1303
Size: 1567 2431
ApaI G_GGCC'C
Cuts at: 121 121
Size: 3998
ApaLI G'TGCA_C
Cuts at: 840 2768 840
Size: 1928 2070
ApoI r'AATT_y
Cuts at: 1002 3351 3607 1002
Size: 2349 256 1393
Fragments arranged by size:
2349 1393 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 1018 1018
Size: 3998
BamHI G'GATC_C
Cuts at: 344 1023 344
Size: 679 3319
BanI G'GyrC_C
Cuts at: 1014 1428 1676 1711 1014
Size: 414 248 35 3301
Fragments arranged by size:
3301 414 248 35
BanII G_rGCy'C
Cuts at: 121 169 182 1012 2042 121
Size: 48 13 830 1030 2077
Fragments arranged by size:
2077 1030 830 48 13
BbeI G_GCGC'C
Cuts at: 1432 1680 1432
Size: 248 3750
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 552 586 90
Size: 34 428 34 3502
Fragments arranged by size:
3502 428 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1896 2657 3998 1896
Size: 761 1341 1896
Fragments arranged by size:
1896 1341 761
BclI T'GATC_A
Cuts at: 914 1518 914
Size: 604 3394
BglII A'GATC_T
Cuts at: 1513 3550 1513
Size: 2037 1961
BlpI GC'TnA_GC
Cuts at: 1110 1110
Size: 3998
Bme1580I G_kGCm'C
Cuts at: 121 844 1623 1716 2772 121
Size: 723 779 93 1056 1347
Fragments arranged by size:
1347 1056 779 723 93
BmrI ACTGGGnnnn_n'
Cuts at: 1210 1291 1626 3770 1210
Size: 81 335 2144 1438
Fragments arranged by size:
2144 1438 335 81
BmtI G_CTAG'C
Cuts at: 578 578
Size: 3998
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 3998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 769 346
Size: 423 3575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 1174 2053 2545 2843 547
Size: 627 879 492 298 1702
Fragments arranged by size:
1702 879 627 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 301 427 745 3702 301
Size: 126 318 2957 597
Fragments arranged by size:
2957 597 318 126
BsaAI yAC'GTr
Cuts at: 1351 1351
Size: 3998
BsaBI GATnn'nnATC
Cuts at: 6 1512 3549 6
Size: 1506 2037 455
Fragments arranged by size:
2037 1506 455
BsaHI Gr'CG_yC
Cuts at: 1429 1677 2379 3875 1429
Size: 248 702 1496 1552
Fragments arranged by size:
1552 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1187 1708 2386 2660 2807 3218 1187
Size: 521 678 274 147 411 1967
Fragments arranged by size:
1967 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 865 865
Size: 3998
BseYI C'CCAG_C
Cuts at: 297 1423 2181 2758 3254 297
Size: 1126 758 577 496 1041
Fragments arranged by size:
1126 1041 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 484
Size: 3998
BsiEI CG_ry'CG
Cuts at: 104 537 1540 1586 2794 104
Size: 433 1003 46 1208 1308
Fragments arranged by size:
1308 1208 1003 433 46
BsiHKAI G_wGCw'C
Cuts at: 397 844 1012 1790 1980 2772 397
Size: 447 168 778 190 792 1623
Fragments arranged by size:
1623 792 778 447 190 168
Bsp1286I G_dGCh'C
Cuts at: 121 169 182 397 844 1012 1623 1716 1790
Size: 48 13 215 447 168 611 93 74
Cuts at: 1790 1980 2042 2772 121
Size: 190 62 730 1347
Fragments arranged by size:
1347 730 611 447 215 190 168 93
74 62 48 13
BspEI T'CCGG_A
Cuts at: 3218 3218
Size: 3998
BspHI T'CATG_A
Cuts at: 356 3174 356
Size: 2818 1180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 254 302 456 1048 1262 1564 1945 254
Size: 48 154 592 214 302 381 2307
Fragments arranged by size:
2307 592 381 302 214 154 48
BsrBI CCG'CTC
Cuts at: 50 2290 2344 3828 3993 50
Size: 2240 54 1484 165 55
Fragments arranged by size:
2240 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1910 1910
Size: 3998
BsrFI r'CCGG_y
Cuts at: 334 1996 2177 2386 334
Size: 1662 181 209 1946
Fragments arranged by size:
1946 1662 209 181
BssHII G'CGCG_C
Cuts at: 172 172
Size: 3998
BssSI C'ACGA_G
Cuts at: 304 2269 2627 304
Size: 1965 358 1675
Fragments arranged by size:
1965 1675 358
BstAPI GCAn_nnn'nTGC
Cuts at: 224 1375 224
Size: 1151 2847
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstXI CCAn_nnnn'nTGG
Cuts at: 297 297
Size: 3998
BstYI r'GATC_y
Cuts at: 1 344 1023 1490 1513 1848 2094 3106 3192
Size: 343 679 467 23 335 246 1012 86
Cuts at: 3192 3204 3550 1
Size: 12 346 449
Fragments arranged by size:
1012 679 467 449 346 343 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 258 258
Size: 3998
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1948 2129 2292 1948
Size: 181 163 3654
Fragments arranged by size:
3654 181 163
BtsI GCAGTG_nn'
Cuts at: 153 370 844 994 1241 153
Size: 217 474 150 247 2910
Fragments arranged by size:
2910 474 247 217 150
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 1704 2562 1704
Size: 858 3140
EaeI y'GGCC_r
Cuts at: 192 239 1583 1757 2148 2175 3520 192
Size: 47 1344 174 391 27 1345 670
Fragments arranged by size:
1345 1344 670 391 174 47 27
EagI C'GGCC_G
Cuts at: 1583 1583
Size: 3998
EarI CTCTTCn'nnn_
Cuts at: 145 187 406 2021 2231 145
Size: 42 219 1615 210 1912
Fragments arranged by size:
1912 1615 219 210 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 486 2206 2516 2662 3808 486
Size: 1720 310 146 1146 676
Fragments arranged by size:
1720 1146 676 310 146
EcoICRI GAG'CTC
Cuts at: 1010 1010
Size: 3998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 769 823 1823 2255 3002 3270 346
Size: 423 54 1000 432 747 268 1074
Fragments arranged by size:
1074 1000 747 432 423 268 54
EcoO109I rG'GnC_Cy
Cuts at: 117 118 798 1137 117
Size: 1 680 339 2978
Fragments arranged by size:
2978 680 339 1
EcoRI G'AATT_C
Cuts at: 1002 1002
Size: 3998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3998
FspI TGC'GCA
Cuts at: 1779 1779
Size: 3998
HaeII r_GCGC'y
Cuts at: 341 1432 1680 2702 3681 341
Size: 1091 248 1022 979 658
Fragments arranged by size:
1091 1022 979 658 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1840 1870 1872 1902 1840
Size: 30 2 30 3936
Fragments arranged by size:
3936 30 30 2
HincII GTy'rAC
Cuts at: 1037 1037
Size: 3998
KasI G'GCGC_C
Cuts at: 1428 1676 1428
Size: 248 3750
KpnI G_GTAC'C
Cuts at: 1018 1018
Size: 3998
MfeI C'AATT_G
Cuts at: 222 222
Size: 3998
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2669 2853 2669
Size: 184 3814
MscI TGG'CCA
Cuts at: 194 1759 194
Size: 1565 2433
MslI CAynn'nnrTG
Cuts at: 922 1987 2114 922
Size: 1065 127 2806
Fragments arranged by size:
2806 1065 127
MspA1I CmG'CkG
Cuts at: 252 351 479 1109 1423 1783 2796 3041 252
Size: 99 128 630 314 360 1013 245 1209
Fragments arranged by size:
1209 1013 630 360 314 245 128 99
NaeI GCC'GGC
Cuts at: 336 2179 336
Size: 1843 2155
NarI GG'CG_CC
Cuts at: 1429 1677 1429
Size: 248 3750
NgoMIV G'CCGG_C
Cuts at: 334 2177 334
Size: 1843 2155
NheI G'CTAG_C
Cuts at: 574 574
Size: 3998
NspI r_CATG'y
Cuts at: 282 525 1051 1551 2458 282
Size: 243 526 500 907 1822
Fragments arranged by size:
1822 907 526 500 243
PciI A'CATG_T
Cuts at: 2454 2454
Size: 3998
PfoI T'CCnGG_A
Cuts at: 746 746
Size: 3998
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3966
PpuMI rG'GwC_Cy
Cuts at: 798 798
Size: 3998
PspOMI G'GGCC_C
Cuts at: 117 117
Size: 3998
PstI C_TGCA'G
Cuts at: 116 251 989 1045 1730 116
Size: 135 738 56 685 2384
Fragments arranged by size:
2384 738 685 135 56
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3998
PvuII CAG'CTG
Cuts at: 252 1423 1783 252
Size: 1171 360 2467
Fragments arranged by size:
2467 1171 360
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 1012 1012
Size: 3998
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 145 187 406 2021 2231 145
Size: 42 219 1615 210 1912
Fragments arranged by size:
1912 1615 219 210 42
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 3515 3515
Size: 3998
SfcI C'TryA_G
Cuts at: 33 112 247 985 1041 1726 2719 2910 33
Size: 79 135 738 56 685 993 191 1121
Fragments arranged by size:
1121 993 738 685 191 135 79 56
SfoI GGC'GCC
Cuts at: 1430 1678 1430
Size: 248 3750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 1020 1020
Size: 3998
SmlI C'TyrA_G
Cuts at: 526 1153 2068 2560 2822 526
Size: 627 915 492 262 1702
Fragments arranged by size:
1702 915 627 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 1051 1551 1051
Size: 500 3498
StyI C'CwwG_G
Cuts at: 1132 1486 1132
Size: 354 3644
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1581 1714 2173 2382 1581
Size: 133 459 209 3197
Fragments arranged by size:
3197 459 209 133
TatI w'GTAC_w
Cuts at: 3513 3513
Size: 3998
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3515 3547 3515
Size: 32 3966
Tth111I GACn'n_nGTC
Cuts at: 1795 1795
Size: 3998
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 744 1493 744
Size: 749 3249
XmaI C'CCGG_G
Cuts at: 1018 1018
Size: 3998
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BbsI BbvCI BfrBI BglI BmgBI Bpu10I BsaXI BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NcoI
NdeI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI