Restriction map for
pF1KB6358
(Circular) (Six-base) MAPSORT of: pF1KB6358.seq Check: 9504 from: 1 to: 4001
pF1KB6358 4001 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 810 810
Size: 4001
AccI GT'mk_AC
Cuts at: 938 1039 938
Size: 101 3900
Acc65I G'GTAC_C
Cuts at: 1017 1017
Size: 4001
AclI AA'CG_TT
Cuts at: 3841 3841
Size: 4001
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1826 2258 3005 3273 1826
Size: 432 747 268 2554
Fragments arranged by size:
2554 747 432 268
AflIII A'CryG_T
Cuts at: 1353 2457 3434 1353
Size: 1104 977 1920
Fragments arranged by size:
1920 1104 977
AgeI A'CCGG_T
Cuts at: 2389 2389
Size: 4001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 493 493
Size: 4001
AlwNI CAG_nnn'CTG
Cuts at: 1306 2873 1306
Size: 1567 2434
ApaI G_GGCC'C
Cuts at: 963 963
Size: 4001
ApaLI G'TGCA_C
Cuts at: 2771 2771
Size: 4001
ApoI r'AATT_y
Cuts at: 1005 3354 3610 1005
Size: 2349 256 1396
Fragments arranged by size:
2349 1396 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4001
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
AvaI C'yCGr_G
Cuts at: 145 259 403 729 962 1021 145
Size: 114 144 326 233 59 3125
Fragments arranged by size:
3125 326 233 144 114 59
BamHI G'GATC_C
Cuts at: 1026 1026
Size: 4001
BanI G'GyrC_C
Cuts at: 358 399 464 1017 1431 1679 1714 358
Size: 41 65 553 414 248 35 2645
Fragments arranged by size:
2645 553 414 248 65 41 35
BanII G_rGCy'C
Cuts at: 764 963 1015 2045 764
Size: 199 52 1030 2720
Fragments arranged by size:
2720 1030 199 52
BbeI G_GCGC'C
Cuts at: 362 468 1435 1683 362
Size: 106 967 248 2680
Fragments arranged by size:
2680 967 248 106
BbvCI CC'TCA_GC
Cuts at: 188 665 188
Size: 477 3524
BciVI GTATCCnnnnn_n'
Cuts at: 1899 2660 4001 1899
Size: 761 1341 1899
Fragments arranged by size:
1899 1341 761
BclI T'GATC_A
Cuts at: 1521 1521
Size: 4001
BglII A'GATC_T
Cuts at: 863 1516 3553 863
Size: 653 2037 1311
Fragments arranged by size:
2037 1311 653
BlpI GC'TnA_GC
Cuts at: 1113 1113
Size: 4001
Bme1580I G_kGCm'C
Cuts at: 404 833 963 1626 1719 2775 404
Size: 429 130 663 93 1056 1630
Fragments arranged by size:
1630 1056 663 429 130 93
BmgBI CAC'GTC
Cuts at: 522 522
Size: 4001
BmrI ACTGGGnnnn_n'
Cuts at: 684 1213 1294 1629 3773 684
Size: 529 81 335 2144 912
Fragments arranged by size:
2144 912 529 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 564 584 564
Size: 20 3981
Bpu10I CC'TnA_GC
Cuts at: 188 454 665 188
Size: 266 211 3524
Fragments arranged by size:
3524 266 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 1177 2056 2548 2846 361
Size: 816 879 492 298 1516
Fragments arranged by size:
1516 879 816 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3705 3705
Size: 4001
BsaAI yAC'GTr
Cuts at: 1354 1354
Size: 4001
BsaBI GATnn'nnATC
Cuts at: 6 862 1515 3552 6
Size: 856 653 2037 455
Fragments arranged by size:
2037 856 653 455
BsaHI Gr'CG_yC
Cuts at: 359 465 896 1432 1680 2382 3878 359
Size: 106 431 536 248 702 1496 482
Fragments arranged by size:
1496 702 536 482 431 248 106
BsaWI w'CCGG_w
Cuts at: 1190 1711 2389 2663 2810 3221 1190
Size: 521 678 274 147 411 1970
Fragments arranged by size:
1970 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 601 631 601
Size: 30 3971
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 178 190 205 208 232 235 433 436
Size: 15 12 15 3 24 3 198 3
Cuts at: 436 568 609 694 163
Size: 132 41 85 3470
Fragments arranged by size:
3470 198 132 85 41 24 15 15
12 3 3 3
BseYI C'CCAG_C
Cuts at: 1426 2184 2761 3257 1426
Size: 758 577 496 2170
Fragments arranged by size:
2170 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 331
Size: 4001
BsiEI CG_ry'CG
Cuts at: 104 202 233 299 1543 1589 2797 104
Size: 98 31 66 1244 46 1208 1308
Fragments arranged by size:
1308 1244 1208 98 66 46 31
BsiHKAI G_wGCw'C
Cuts at: 764 788 920 1015 1793 1983 2775 764
Size: 24 132 95 778 190 792 1990
Fragments arranged by size:
1990 792 778 190 132 95 24
BsmBI CGTCTCn'nnnn_
Cuts at: 110 110
Size: 4001
Bsp1286I G_dGCh'C
Cuts at: 404 764 788 833 920 963 1015 1626 1719
Size: 360 24 45 87 43 52 611 93
Cuts at: 1719 1793 1983 2045 2775 404
Size: 74 190 62 730 1630
Fragments arranged by size:
1630 730 611 360 190 93 87 74
62 52 45 43 24
BspEI T'CCGG_A
Cuts at: 3221 3221
Size: 4001
BspHI T'CATG_A
Cuts at: 3177 3177
Size: 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 665 810 1051 1265 1567 1948 665
Size: 145 241 214 302 381 2718
Fragments arranged by size:
2718 381 302 241 214 145
BsrBI CCG'CTC
Cuts at: 50 198 2293 2347 3831 3996 50
Size: 148 2095 54 1484 165 55
Fragments arranged by size:
2095 1484 165 148 55 54
BsrDI GCAATG_nn'
Cuts at: 1913 1913
Size: 4001
BsrFI r'CCGG_y
Cuts at: 294 702 1999 2180 2389 294
Size: 408 1297 181 209 1906
Fragments arranged by size:
1906 1297 408 209 181
BssSI C'ACGA_G
Cuts at: 787 2272 2630 787
Size: 1485 358 2158
Fragments arranged by size:
2158 1485 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1378 1378
Size: 4001
BstBI TT'CG_AA
Cuts at: 2362 2362
Size: 4001
BstEII G'GTnAC_C
Cuts at: 121 966 121
Size: 845 3156
BstYI r'GATC_y
Cuts at: 1 863 1026 1493 1516 1851 2097 3109 3195
Size: 862 163 467 23 335 246 1012 86
Cuts at: 3195 3207 3553 1
Size: 12 346 449
Fragments arranged by size:
1012 862 467 449 346 335 246 163
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4001
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1951 2132 2295 1951
Size: 181 163 3657
Fragments arranged by size:
3657 181 163
BtsI GCAGTG_nn'
Cuts at: 289 833 1244 289
Size: 544 411 3046
Fragments arranged by size:
3046 544 411
DraI TTT'AAA
Cuts at: 1000 1000
Size: 4001
DrdI GACnn_nn'nnGTC
Cuts at: 1707 2565 1707
Size: 858 3143
EaeI y'GGCC_r
Cuts at: 199 230 284 296 704 1586 1760 2151 2178
Size: 31 54 12 408 882 174 391 27
Cuts at: 2178 3523 199
Size: 1345 677
Fragments arranged by size:
1345 882 677 408 391 174 54 31
27 12
EagI C'GGCC_G
Cuts at: 199 230 296 1586 199
Size: 31 66 1290 2614
Fragments arranged by size:
2614 1290 66 31
EarI CTCTTCn'nnn_
Cuts at: 491 2024 2234 491
Size: 1533 210 2258
Fragments arranged by size:
2258 1533 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2209 2519 2665 3811 2209
Size: 310 146 1146 2399
Fragments arranged by size:
2399 1146 310 146
EcoICRI GAG'CTC
Cuts at: 762 1013 762
Size: 251 3750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 564 584 1826 2258 3005 3273 564
Size: 20 1242 432 747 268 1292
Fragments arranged by size:
1292 1242 747 432 268 20
EcoO109I rG'GnC_Cy
Cuts at: 959 1140 959
Size: 181 3820
EcoRI G'AATT_C
Cuts at: 1005 1005
Size: 4001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 627 2020 627
Size: 1393 2608
FspI TGC'GCA
Cuts at: 1782 1782
Size: 4001
HaeII r_GCGC'y
Cuts at: 362 468 1435 1683 2705 3684 362
Size: 106 967 248 1022 979 679
Fragments arranged by size:
1022 979 967 679 248 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 712 744 1843 1873 1875 1905 712
Size: 32 1099 30 2 30 2808
Fragments arranged by size:
2808 1099 32 30 30 2
HincII GTy'rAC
Cuts at: 1040 1040
Size: 4001
KasI G'GCGC_C
Cuts at: 358 464 1431 1679 358
Size: 106 967 248 2680
Fragments arranged by size:
2680 967 248 106
KpnI G_GTAC'C
Cuts at: 1021 1021
Size: 4001
MluI A'CGCG_T
Cuts at: 3434 3434
Size: 4001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2672 2856 2672
Size: 184 3817
MscI TGG'CCA
Cuts at: 1762 1762
Size: 4001
MslI CAynn'nnrTG
Cuts at: 712 1990 2117 712
Size: 1278 127 2596
Fragments arranged by size:
2596 1278 127
MspA1I CmG'CkG
Cuts at: 267 366 815 1112 1426 1786 2799 3044 267
Size: 99 449 297 314 360 1013 245 1224
Fragments arranged by size:
1224 1013 449 360 314 297 245 99
NaeI GCC'GGC
Cuts at: 296 704 2182 296
Size: 408 1478 2115
Fragments arranged by size:
2115 1478 408
NarI GG'CG_CC
Cuts at: 359 465 1432 1680 359
Size: 106 967 248 2680
Fragments arranged by size:
2680 967 248 106
NcoI C'CATG_G
Cuts at: 107 107
Size: 4001
NgoMIV G'CCGG_C
Cuts at: 294 702 2180 294
Size: 408 1478 2115
Fragments arranged by size:
2115 1478 408
NotI GC'GGCC_GC
Cuts at: 230 230
Size: 4001
NspI r_CATG'y
Cuts at: 1054 1554 2461 1054
Size: 500 907 2594
Fragments arranged by size:
2594 907 500
PciI A'CATG_T
Cuts at: 2457 2457
Size: 4001
PmeI GTTT'AAAC
Cuts at: 1000 1000
Size: 4001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3969
PspOMI G'GGCC_C
Cuts at: 959 959
Size: 4001
PstI C_TGCA'G
Cuts at: 662 1048 1733 662
Size: 386 685 2930
Fragments arranged by size:
2930 685 386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4001
PvuII CAG'CTG
Cuts at: 1426 1786 1426
Size: 360 3641
RsrII CG'GwC_CG
Cuts at: 2196 2196
Size: 4001
SacI G_AGCT'C
Cuts at: 764 1015 764
Size: 251 3750
SalI G'TCGA_C
Cuts at: 1038 1038
Size: 4001
SapI GCTCTTCn'nnn_
Cuts at: 2024 2234 2024
Size: 210 3791
SbfI CC_TGCA'GG
Cuts at: 662 1048 662
Size: 386 3615
ScaI AGT'ACT
Cuts at: 610 3518 610
Size: 2908 1093
SfcI C'TryA_G
Cuts at: 33 658 1044 1729 2722 2913 33
Size: 625 386 685 993 191 1121
Fragments arranged by size:
1121 993 685 625 386 191
SfoI GGC'GCC
Cuts at: 360 466 1433 1681 360
Size: 106 967 248 2680
Fragments arranged by size:
2680 967 248 106
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
SmaI CCC'GGG
Cuts at: 964 1023 964
Size: 59 3942
SmlI C'TyrA_G
Cuts at: 376 1156 2071 2563 2825 376
Size: 780 915 492 262 1552
Fragments arranged by size:
1552 915 780 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4001
SphI G_CATG'C
Cuts at: 1054 1554 1054
Size: 500 3501
StuI AGG'CCT
Cuts at: 316 316
Size: 4001
StyI C'CwwG_G
Cuts at: 107 1135 1489 107
Size: 1028 354 2619
Fragments arranged by size:
2619 1028 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 601 1584 1717 2176 2385 601
Size: 983 133 459 209 2217
Fragments arranged by size:
2217 983 459 209 133
TatI w'GTAC_w
Cuts at: 608 3516 608
Size: 2908 1093
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 499 531 3518 3550 499
Size: 32 2987 32 950
Fragments arranged by size:
2987 950 32 32
Tth111I GACn'n_nGTC
Cuts at: 934 1798 934
Size: 864 3137
XbaI T'CTAG_A
Cuts at: 1032 1032
Size: 4001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1496 1496
Size: 4001
XmaI C'CCGG_G
Cuts at: 962 1021 962
Size: 59 3942
XmnI GAAnn'nnTTC
Cuts at: 610 610
Size: 4001
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BglI BmtI BsiWI BsmI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI