Restriction map for
pF1KB6359
(Circular) (Six-base) MAPSORT of: pF1KB6359.seq Check: 5421 from: 1 to: 3875
pF1KB6359 3875 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 3715 3715
Size: 3875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 262 1700 2132 2879 3147 142
Size: 120 1438 432 747 268 870
Fragments arranged by size:
1438 870 747 432 268 120
AflIII A'CryG_T
Cuts at: 1227 2331 3308 1227
Size: 1104 977 1794
Fragments arranged by size:
1794 1104 977
AgeI A'CCGG_T
Cuts at: 2263 2263
Size: 3875
AlwNI CAG_nnn'CTG
Cuts at: 307 450 534 1180 2747 307
Size: 143 84 646 1567 1435
Fragments arranged by size:
1567 1435 646 143 84
ApaLI G'TGCA_C
Cuts at: 2645 2645
Size: 3875
ApoI r'AATT_y
Cuts at: 201 879 3228 3484 201
Size: 678 2349 256 592
Fragments arranged by size:
2349 678 592 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3875
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 895 895
Size: 3875
BamHI G'GATC_C
Cuts at: 900 900
Size: 3875
BanI G'GyrC_C
Cuts at: 891 1305 1553 1588 891
Size: 414 248 35 3178
Fragments arranged by size:
3178 414 248 35
BanII G_rGCy'C
Cuts at: 889 1919 889
Size: 1030 2845
BbeI G_GCGC'C
Cuts at: 1309 1557 1309
Size: 248 3627
BbsI GAAGACnn'nnnn_
Cuts at: 194 194
Size: 3875
BbvCI CC'TCA_GC
Cuts at: 778 778
Size: 3875
BciVI GTATCCnnnnn_n'
Cuts at: 1773 2534 3875 1773
Size: 761 1341 1773
Fragments arranged by size:
1773 1341 761
BclI T'GATC_A
Cuts at: 1395 1395
Size: 3875
BglII A'GATC_T
Cuts at: 1390 3427 1390
Size: 2037 1838
BlpI GC'TnA_GC
Cuts at: 987 987
Size: 3875
Bme1580I G_kGCm'C
Cuts at: 1500 1593 2649 1500
Size: 93 1056 2726
Fragments arranged by size:
2726 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 295 1087 1168 1503 3647 295
Size: 792 81 335 2144 523
Fragments arranged by size:
2144 792 523 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 3875
Bpu10I CC'TnA_GC
Cuts at: 778 778
Size: 3875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 1051 1930 2422 2720 130
Size: 921 879 492 298 1285
Fragments arranged by size:
1285 921 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3579 3579
Size: 3875
BsaAI yAC'GTr
Cuts at: 1228 1228
Size: 3875
BsaBI GATnn'nnATC
Cuts at: 6 1389 3426 6
Size: 1383 2037 455
Fragments arranged by size:
2037 1383 455
BsaHI Gr'CG_yC
Cuts at: 1306 1554 2256 3752 1306
Size: 248 702 1496 1429
Fragments arranged by size:
1496 1429 702 248
BsaWI w'CCGG_w
Cuts at: 1064 1585 2263 2537 2684 3095 1064
Size: 521 678 274 147 411 1844
Fragments arranged by size:
1844 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1300 2058 2635 3131 1300
Size: 758 577 496 2044
Fragments arranged by size:
2044 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1417 1463 2671 104
Size: 1313 46 1208 1308
Fragments arranged by size:
1313 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 889 1667 1857 2649 889
Size: 778 190 792 2115
Fragments arranged by size:
2115 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 889 1500 1593 1667 1857 1919 2649 889
Size: 611 93 74 190 62 730 2115
Fragments arranged by size:
2115 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3095 3095
Size: 3875
BspHI T'CATG_A
Cuts at: 3051 3051
Size: 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 925 1139 1441 1822 925
Size: 214 302 381 2978
Fragments arranged by size:
2978 381 302 214
BsrBI CCG'CTC
Cuts at: 50 168 2167 2221 3705 3870 50
Size: 118 1999 54 1484 165 55
Fragments arranged by size:
1999 1484 165 118 55 54
BsrDI GCAATG_nn'
Cuts at: 858 1787 858
Size: 929 2946
BsrFI r'CCGG_y
Cuts at: 1873 2054 2263 1873
Size: 181 209 3485
Fragments arranged by size:
3485 209 181
BssSI C'ACGA_G
Cuts at: 2146 2504 2146
Size: 358 3517
BstAPI GCAn_nnn'nTGC
Cuts at: 1252 1252
Size: 3875
BstBI TT'CG_AA
Cuts at: 2236 2236
Size: 3875
BstYI r'GATC_y
Cuts at: 1 278 482 900 1367 1390 1725 1971 2983
Size: 277 204 418 467 23 335 246 1012
Cuts at: 2983 3069 3081 3427 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 418 346 335 277 246
204 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3875
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1825 2006 2169 1825
Size: 181 163 3531
Fragments arranged by size:
3531 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 1118
Size: 3875
DraI TTT'AAA
Cuts at: 874 874
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 347 1581 2439 347
Size: 1234 858 1783
Fragments arranged by size:
1783 1234 858
EaeI y'GGCC_r
Cuts at: 1460 1634 2025 2052 3397 1460
Size: 174 391 27 1345 1938
Fragments arranged by size:
1938 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1460 1460
Size: 3875
EarI CTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2083 2393 2539 3685 2083
Size: 310 146 1146 2273
Fragments arranged by size:
2273 1146 310 146
EcoICRI GAG'CTC
Cuts at: 887 887
Size: 3875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 262 1700 2132 2879 3147 142
Size: 120 1438 432 747 268 870
Fragments arranged by size:
1438 870 747 432 268 120
EcoO109I rG'GnC_Cy
Cuts at: 1014 1014
Size: 3875
EcoRI G'AATT_C
Cuts at: 879 879
Size: 3875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3875
FspI TGC'GCA
Cuts at: 1656 1656
Size: 3875
HaeII r_GCGC'y
Cuts at: 1309 1557 2579 3558 1309
Size: 248 1022 979 1626
Fragments arranged by size:
1626 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1717 1747 1749 1779 1717
Size: 30 2 30 3813
Fragments arranged by size:
3813 30 30 2
HincII GTy'rAC
Cuts at: 914 914
Size: 3875
KasI G'GCGC_C
Cuts at: 1305 1553 1305
Size: 248 3627
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 353 2546 2730 353
Size: 2193 184 1498
Fragments arranged by size:
2193 1498 184
MscI TGG'CCA
Cuts at: 1636 1636
Size: 3875
MslI CAynn'nnrTG
Cuts at: 1864 1991 1864
Size: 127 3748
MspA1I CmG'CkG
Cuts at: 450 986 1300 1660 2673 2918 450
Size: 536 314 360 1013 245 1407
Fragments arranged by size:
1407 1013 536 360 314 245
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 3875
NarI GG'CG_CC
Cuts at: 1306 1554 1306
Size: 248 3627
NcoI C'CATG_G
Cuts at: 107 107
Size: 3875
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 3875
NspI r_CATG'y
Cuts at: 928 1428 2335 928
Size: 500 907 2468
Fragments arranged by size:
2468 907 500
PciI A'CATG_T
Cuts at: 2331 2331
Size: 3875
PflMI CCAn_nnn'nTGG
Cuts at: 450 450
Size: 3875
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3843
PsiI TTA'TAA
Cuts at: 359 359
Size: 3875
PstI C_TGCA'G
Cuts at: 922 1607 922
Size: 685 3190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 450 1300 1660 450
Size: 850 360 2665
Fragments arranged by size:
2665 850 360
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 889 889
Size: 3875
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SfcI C'TryA_G
Cuts at: 33 918 1603 2596 2787 33
Size: 885 685 993 191 1121
Fragments arranged by size:
1121 993 885 685 191
SfoI GGC'GCC
Cuts at: 1307 1555 1307
Size: 248 3627
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 897 897
Size: 3875
SmlI C'TyrA_G
Cuts at: 145 1030 1945 2437 2699 145
Size: 885 915 492 262 1321
Fragments arranged by size:
1321 915 885 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
StyI C'CwwG_G
Cuts at: 107 396 404 1009 1363 107
Size: 289 8 605 354 2619
Fragments arranged by size:
2619 605 354 289 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1458 1591 2050 2259 1458
Size: 133 459 209 3074
Fragments arranged by size:
3074 459 209 133
TatI w'GTAC_w
Cuts at: 3390 3390
Size: 3875
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 162 194 392 424 3392 3424 162
Size: 32 198 32 2968 32 613
Fragments arranged by size:
2968 613 198 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1672 1672
Size: 3875
XbaI T'CTAG_A
Cuts at: 646 906 646
Size: 260 3615
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1370 1370
Size: 3875
XmaI C'CCGG_G
Cuts at: 895 895
Size: 3875
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI