Restriction map for
pF1KB6360
(Circular) (Six-base) MAPSORT of: pF1KB6360.seq Check: 995 from: 1 to: 4004
pF1KB6360 4004 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 550 550
Size: 4004
AccI GT'mk_AC
Cuts at: 1042 1042
Size: 4004
Acc65I G'GTAC_C
Cuts at: 237 1020 237
Size: 783 3221
AclI AA'CG_TT
Cuts at: 998 3844 998
Size: 2846 1158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1829 2261 3008 3276 946
Size: 883 432 747 268 1674
Fragments arranged by size:
1674 883 747 432 268
AflIII A'CryG_T
Cuts at: 1356 2460 3437 1356
Size: 1104 977 1923
Fragments arranged by size:
1923 1104 977
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 824 824
Size: 4004
AlwNI CAG_nnn'CTG
Cuts at: 436 924 1309 2876 436
Size: 488 385 1567 1564
Fragments arranged by size:
1567 1564 488 385
ApaLI G'TGCA_C
Cuts at: 2774 2774
Size: 4004
ApoI r'AATT_y
Cuts at: 587 774 1008 3357 3613 587
Size: 187 234 2349 256 978
Fragments arranged by size:
2349 978 256 234 187
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 1024 1024
Size: 4004
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 229 262 229
Size: 33 3971
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 237 643 1020 1434 1682 1717 237
Size: 406 377 414 248 35 2524
Fragments arranged by size:
2524 414 406 377 248 35
BanII G_rGCy'C
Cuts at: 1018 2048 1018
Size: 1030 2974
BbeI G_GCGC'C
Cuts at: 1438 1686 1438
Size: 248 3756
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 1524 1524
Size: 4004
BglII A'GATC_T
Cuts at: 1519 3556 1519
Size: 2037 1967
BlpI GC'TnA_GC
Cuts at: 1116 1116
Size: 4004
Bme1580I G_kGCm'C
Cuts at: 1629 1722 2778 1629
Size: 93 1056 2855
Fragments arranged by size:
2855 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 184 1216 1297 1632 3776 184
Size: 1032 81 335 2144 412
Fragments arranged by size:
2144 1032 412 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 732 2371 732
Size: 1639 2365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 2059 2551 2849 1180
Size: 879 492 298 2335
Fragments arranged by size:
2335 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3708 3708
Size: 4004
BsaAI yAC'GTr
Cuts at: 1357 1357
Size: 4004
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 1435 1683 2385 3881 1435
Size: 248 702 1496 1558
Fragments arranged by size:
1558 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1193 1714 2392 2666 2813 3224 1193
Size: 521 678 274 147 411 1973
Fragments arranged by size:
1973 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 133
Size: 4004
BseYI C'CCAG_C
Cuts at: 455 743 1429 2187 2764 3260 455
Size: 288 686 758 577 496 1199
Fragments arranged by size:
1199 758 686 577 496 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 578 421
Size: 157 3847
BsiEI CG_ry'CG
Cuts at: 104 1546 1592 2800 104
Size: 1442 46 1208 1308
Fragments arranged by size:
1442 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1018 1796 1986 2778 1018
Size: 778 190 792 2244
Fragments arranged by size:
2244 792 778 190
BsmI GAATG_Cn'
Cuts at: 813 813
Size: 4004
Bsp1286I G_dGCh'C
Cuts at: 1018 1629 1722 1796 1986 2048 2778 1018
Size: 611 93 74 190 62 730 2244
Fragments arranged by size:
2244 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3224 3224
Size: 4004
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 550 1054 1268 1570 1951 550
Size: 504 214 302 381 2603
Fragments arranged by size:
2603 504 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2296 2350 3834 3999 50
Size: 2246 54 1484 165 55
Fragments arranged by size:
2246 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1916 1916
Size: 4004
BsrFI r'CCGG_y
Cuts at: 2002 2183 2392 2002
Size: 181 209 3614
Fragments arranged by size:
3614 209 181
BsrGI T'GTAC_A
Cuts at: 577 577
Size: 4004
BssSI C'ACGA_G
Cuts at: 2275 2633 2275
Size: 358 3646
BstAPI GCAn_nnn'nTGC
Cuts at: 1381 1381
Size: 4004
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstYI r'GATC_y
Cuts at: 1 420 1029 1496 1519 1854 2100 3112 3198
Size: 419 609 467 23 335 246 1012 86
Cuts at: 3198 3210 3556 1
Size: 12 346 449
Fragments arranged by size:
1012 609 467 449 419 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1954 2135 2298 1954
Size: 181 163 3660
Fragments arranged by size:
3660 181 163
BtsI GCAGTG_nn'
Cuts at: 168 1247 168
Size: 1079 2925
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DraIII CAC_nnn'GTG
Cuts at: 861 861
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 1589 1763 2154 2181 3526 1589
Size: 174 391 27 1345 2067
Fragments arranged by size:
2067 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1589 1589
Size: 4004
EarI CTCTTCn'nnn_
Cuts at: 110 701 839 2027 2237 110
Size: 591 138 1188 210 1877
Fragments arranged by size:
1877 1188 591 210 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 889 2212 2522 2668 3814 889
Size: 1323 310 146 1146 1079
Fragments arranged by size:
1323 1146 1079 310 146
EcoICRI GAG'CTC
Cuts at: 1016 1016
Size: 4004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1829 2261 3008 3276 946
Size: 883 432 747 268 1674
Fragments arranged by size:
1674 883 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1143 1143
Size: 4004
EcoRI G'AATT_C
Cuts at: 774 1008 774
Size: 234 3770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 4004
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 1438 1686 2708 3687 1438
Size: 248 1022 979 1755
Fragments arranged by size:
1755 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 732 764 1846 1876 1878 1908 732
Size: 32 1082 30 2 30 2828
Fragments arranged by size:
2828 1082 32 30 30 2
HincII GTy'rAC
Cuts at: 851 1043 851
Size: 192 3812
HindIII A'AGCT_T
Cuts at: 337 337
Size: 4004
HpaI GTT'AAC
Cuts at: 851 851
Size: 4004
KasI G'GCGC_C
Cuts at: 1434 1682 1434
Size: 248 3756
KpnI G_GTAC'C
Cuts at: 241 1024 241
Size: 783 3221
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2859 2675
Size: 184 3820
MscI TGG'CCA
Cuts at: 1765 1765
Size: 4004
MslI CAynn'nnrTG
Cuts at: 1993 2120 1993
Size: 127 3877
MspA1I CmG'CkG
Cuts at: 1115 1429 1789 2802 3047 1115
Size: 314 360 1013 245 2072
Fragments arranged by size:
2072 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 1435 1683 1435
Size: 248 3756
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NspI r_CATG'y
Cuts at: 1057 1557 2464 1057
Size: 500 907 2597
Fragments arranged by size:
2597 907 500
PciI A'CATG_T
Cuts at: 2460 2460
Size: 4004
PfoI T'CCnGG_A
Cuts at: 830 830
Size: 4004
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3972
PstI C_TGCA'G
Cuts at: 1051 1736 1051
Size: 685 3319
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 1429 1789 1429
Size: 360 3644
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 1018 1018
Size: 4004
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 351 3521 351
Size: 3170 834
SfcI C'TryA_G
Cuts at: 33 820 1047 1732 2725 2916 33
Size: 787 227 685 993 191 1121
Fragments arranged by size:
1121 993 787 685 227 191
SfoI GGC'GCC
Cuts at: 1436 1684 1436
Size: 248 3756
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 1026 1026
Size: 4004
SmlI C'TyrA_G
Cuts at: 1159 2074 2566 2828 1159
Size: 915 492 262 2335
Fragments arranged by size:
2335 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
SspI AAT'ATT
Cuts at: 228 532 228
Size: 304 3700
StyI C'CwwG_G
Cuts at: 1138 1492 1138
Size: 354 3650
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1587 1720 2179 2388 1587
Size: 133 459 209 3203
Fragments arranged by size:
3203 459 209 133
TatI w'GTAC_w
Cuts at: 349 577 3519 349
Size: 228 2942 834
Fragments arranged by size:
2942 834 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 3972
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1499 1499
Size: 4004
XmaI C'CCGG_G
Cuts at: 1024 1024
Size: 4004
XmnI GAAnn'nnTTC
Cuts at: 920 920
Size: 4004
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI
FspAI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI