Restriction map for
pF1KB6363
(Circular) (Six-base) MAPSORT of: pF1KB6363.seq Check: 1019 from: 1 to: 3914
pF1KB6363 3914 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 952 952
Size: 3914
Acc65I G'GTAC_C
Cuts at: 930 930
Size: 3914
AclI AA'CG_TT
Cuts at: 3754 3754
Size: 3914
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1739 2171 2918 3186 466
Size: 1273 432 747 268 1194
Fragments arranged by size:
1273 1194 747 432 268
AflIII A'CryG_T
Cuts at: 1266 2370 3347 1266
Size: 1104 977 1833
Fragments arranged by size:
1833 1104 977
AgeI A'CCGG_T
Cuts at: 2302 2302
Size: 3914
AlwNI CAG_nnn'CTG
Cuts at: 1219 2786 1219
Size: 1567 2347
ApaI G_GGCC'C
Cuts at: 420 420
Size: 3914
ApaLI G'TGCA_C
Cuts at: 2684 2684
Size: 3914
ApoI r'AATT_y
Cuts at: 918 3267 3523 918
Size: 2349 256 1309
Fragments arranged by size:
2349 1309 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3914
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
AvaI C'yCGr_G
Cuts at: 934 934
Size: 3914
BamHI G'GATC_C
Cuts at: 206 939 206
Size: 733 3181
BanI G'GyrC_C
Cuts at: 334 930 1344 1592 1627 334
Size: 596 414 248 35 2621
Fragments arranged by size:
2621 596 414 248 35
BanII G_rGCy'C
Cuts at: 115 420 479 599 836 928 1958 115
Size: 305 59 120 237 92 1030 2071
Fragments arranged by size:
2071 1030 305 237 120 92 59
BbeI G_GCGC'C
Cuts at: 338 1348 1596 338
Size: 1010 248 2656
Fragments arranged by size:
2656 1010 248
BbsI GAAGACnn'nnnn_
Cuts at: 273 890 273
Size: 617 3297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 783 817 783
Size: 34 3880
BciVI GTATCCnnnnn_n'
Cuts at: 1812 2573 3914 1812
Size: 761 1341 1812
Fragments arranged by size:
1812 1341 761
BclI T'GATC_A
Cuts at: 1434 1434
Size: 3914
BglII A'GATC_T
Cuts at: 1429 3466 1429
Size: 2037 1877
BlpI GC'TnA_GC
Cuts at: 114 593 1026 114
Size: 479 433 3002
Fragments arranged by size:
3002 479 433
Bme1580I G_kGCm'C
Cuts at: 420 1539 1632 2688 420
Size: 1119 93 1056 1646
Fragments arranged by size:
1646 1119 1056 93
BmgBI CAC'GTC
Cuts at: 873 873
Size: 3914
BmrI ACTGGGnnnn_n'
Cuts at: 1126 1207 1542 3686 1126
Size: 81 335 2144 1354
Fragments arranged by size:
2144 1354 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 3914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 237
Size: 3914
Bpu10I CC'TnA_GC
Cuts at: 262 542 262
Size: 280 3634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1090 1969 2461 2759 1090
Size: 879 492 298 2245
Fragments arranged by size:
2245 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3618 3618
Size: 3914
BsaAI yAC'GTr
Cuts at: 534 1267 534
Size: 733 3181
BsaBI GATnn'nnATC
Cuts at: 6 1428 3465 6
Size: 1422 2037 455
Fragments arranged by size:
2037 1422 455
BsaHI Gr'CG_yC
Cuts at: 335 1345 1593 2295 3791 335
Size: 1010 248 702 1496 458
Fragments arranged by size:
1496 1010 702 458 248
BsaWI w'CCGG_w
Cuts at: 1103 1624 2302 2576 2723 3134 1103
Size: 521 678 274 147 411 1883
Fragments arranged by size:
1883 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 240 240
Size: 3914
BseYI C'CCAG_C
Cuts at: 360 560 1339 2097 2674 3170 360
Size: 200 779 758 577 496 1104
Fragments arranged by size:
1104 779 758 577 496 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 787 556
Size: 231 3683
BsiEI CG_ry'CG
Cuts at: 104 413 1456 1502 2710 104
Size: 309 1043 46 1208 1308
Fragments arranged by size:
1308 1208 1043 309 46
BsiHKAI G_wGCw'C
Cuts at: 479 599 836 928 1706 1896 2688 479
Size: 120 237 92 778 190 792 1705
Fragments arranged by size:
1705 792 778 237 190 120 92
Bsp1286I G_dGCh'C
Cuts at: 115 420 479 599 836 928 1539 1632 1706
Size: 305 59 120 237 92 611 93 74
Cuts at: 1706 1896 1958 2688 115
Size: 190 62 730 1341
Fragments arranged by size:
1341 730 611 305 237 190 120 93
92 74 62 59
BspEI T'CCGG_A
Cuts at: 3134 3134
Size: 3914
BspHI T'CATG_A
Cuts at: 599 3090 599
Size: 2491 1423
BspMI ACCTGCnnnn'nnnn_
Cuts at: 964 1178 1480 1861 964
Size: 214 302 381 3017
Fragments arranged by size:
3017 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2206 2260 3744 3909 50
Size: 2156 54 1484 165 55
Fragments arranged by size:
2156 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 395 1826 395
Size: 1431 2483
BsrFI r'CCGG_y
Cuts at: 866 1912 2093 2302 866
Size: 1046 181 209 2478
Fragments arranged by size:
2478 1046 209 181
BssSI C'ACGA_G
Cuts at: 2185 2543 2185
Size: 358 3556
BstAPI GCAn_nnn'nTGC
Cuts at: 1291 1291
Size: 3914
BstBI TT'CG_AA
Cuts at: 2275 2275
Size: 3914
BstEII G'GTnAC_C
Cuts at: 615 615
Size: 3914
BstYI r'GATC_y
Cuts at: 1 206 939 1406 1429 1764 2010 3022 3108
Size: 205 733 467 23 335 246 1012 86
Cuts at: 3108 3120 3466 1
Size: 12 346 449
Fragments arranged by size:
1012 733 467 449 346 335 246 205
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 611 725 611
Size: 114 3800
BtgI C'CryG_G
Cuts at: 107 107
Size: 3914
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1864 2045 2208 1864
Size: 181 163 3570
Fragments arranged by size:
3570 181 163
BtsI GCAGTG_nn'
Cuts at: 188 1157 188
Size: 969 2945
DraI TTT'AAA
Cuts at: 913 913
Size: 3914
DrdI GACnn_nn'nnGTC
Cuts at: 1620 2478 1620
Size: 858 3056
EaeI y'GGCC_r
Cuts at: 212 410 821 1499 1673 2064 2091 3436 212
Size: 198 411 678 174 391 27 1345 690
Fragments arranged by size:
1345 690 678 411 391 198 174 27
EagI C'GGCC_G
Cuts at: 410 1499 410
Size: 1089 2825
EarI CTCTTCn'nnn_
Cuts at: 151 157 427 644 1937 2147 151
Size: 6 270 217 1293 210 1918
Fragments arranged by size:
1918 1293 270 217 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2122 2432 2578 3724 2122
Size: 310 146 1146 2312
Fragments arranged by size:
2312 1146 310 146
EcoICRI GAG'CTC
Cuts at: 477 597 834 926 477
Size: 120 237 92 3465
Fragments arranged by size:
3465 237 120 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 466 1739 2171 2918 3186 237
Size: 229 1273 432 747 268 965
Fragments arranged by size:
1273 965 747 432 268 229
EcoO109I rG'GnC_Cy
Cuts at: 272 416 417 690 704 897 1053 272
Size: 144 1 273 14 193 156 3133
Fragments arranged by size:
3133 273 193 156 144 14 1
EcoRI G'AATT_C
Cuts at: 918 918
Size: 3914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1933 1933
Size: 3914
FspI TGC'GCA
Cuts at: 1695 1695
Size: 3914
HaeII r_GCGC'y
Cuts at: 338 1348 1596 2618 3597 338
Size: 1010 248 1022 979 655
Fragments arranged by size:
1022 1010 979 655 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1756 1786 1788 1818 1756
Size: 30 2 30 3852
Fragments arranged by size:
3852 30 30 2
HincII GTy'rAC
Cuts at: 342 953 342
Size: 611 3303
KasI G'GCGC_C
Cuts at: 334 1344 1592 334
Size: 1010 248 2656
Fragments arranged by size:
2656 1010 248
KpnI G_GTAC'C
Cuts at: 934 934
Size: 3914
MluI A'CGCG_T
Cuts at: 3347 3347
Size: 3914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2585 2769 2585
Size: 184 3730
MscI TGG'CCA
Cuts at: 823 1675 823
Size: 852 3062
MslI CAynn'nnrTG
Cuts at: 289 1903 2030 289
Size: 1614 127 2173
Fragments arranged by size:
2173 1614 127
MspA1I CmG'CkG
Cuts at: 353 1025 1339 1699 2712 2957 353
Size: 672 314 360 1013 245 1310
Fragments arranged by size:
1310 1013 672 360 314 245
NaeI GCC'GGC
Cuts at: 868 2095 868
Size: 1227 2687
NarI GG'CG_CC
Cuts at: 335 1345 1593 335
Size: 1010 248 2656
Fragments arranged by size:
2656 1010 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3914
NgoMIV G'CCGG_C
Cuts at: 866 2093 866
Size: 1227 2687
NruI TCG'CGA
Cuts at: 503 503
Size: 3914
NspI r_CATG'y
Cuts at: 967 1467 2374 967
Size: 500 907 2507
Fragments arranged by size:
2507 907 500
PciI A'CATG_T
Cuts at: 2370 2370
Size: 3914
PmeI GTTT'AAAC
Cuts at: 913 913
Size: 3914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3053 3085 3053
Size: 32 3882
PpuMI rG'GwC_Cy
Cuts at: 704 897 704
Size: 193 3721
PspOMI G'GGCC_C
Cuts at: 416 416
Size: 3914
PstI C_TGCA'G
Cuts at: 518 782 961 1646 518
Size: 264 179 685 2786
Fragments arranged by size:
2786 685 264 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3914
PvuII CAG'CTG
Cuts at: 1339 1699 1339
Size: 360 3554
RsrII CG'GwC_CG
Cuts at: 2109 2109
Size: 3914
SacI G_AGCT'C
Cuts at: 479 599 836 928 479
Size: 120 237 92 3465
Fragments arranged by size:
3465 237 120 92
SalI G'TCGA_C
Cuts at: 951 951
Size: 3914
SapI GCTCTTCn'nnn_
Cuts at: 1937 2147 1937
Size: 210 3704
SbfI CC_TGCA'GG
Cuts at: 518 961 518
Size: 443 3471
ScaI AGT'ACT
Cuts at: 3431 3431
Size: 3914
SfcI C'TryA_G
Cuts at: 33 514 778 888 957 1642 2635 2826 33
Size: 481 264 110 69 685 993 191 1121
Fragments arranged by size:
1121 993 685 481 264 191 110 69
SfoI GGC'GCC
Cuts at: 336 1346 1594 336
Size: 1010 248 2656
Fragments arranged by size:
2656 1010 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
SmaI CCC'GGG
Cuts at: 936 936
Size: 3914
SmlI C'TyrA_G
Cuts at: 1069 1984 2476 2738 1069
Size: 915 492 262 2245
Fragments arranged by size:
2245 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3914
SphI G_CATG'C
Cuts at: 967 1467 967
Size: 500 3414
StyI C'CwwG_G
Cuts at: 107 1048 1402 107
Size: 941 354 2619
Fragments arranged by size:
2619 941 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 805 1497 1630 2089 2298 805
Size: 692 133 459 209 2421
Fragments arranged by size:
2421 692 459 209 133
TatI w'GTAC_w
Cuts at: 368 3429 368
Size: 3061 853
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 696 728 862 894 3431 3463 696
Size: 32 134 32 2537 32 1147
Fragments arranged by size:
2537 1147 134 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1711 1711
Size: 3914
XbaI T'CTAG_A
Cuts at: 945 945
Size: 3914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1409 1409
Size: 3914
XmaI C'CCGG_G
Cuts at: 934 934
Size: 3914
XmnI GAAnn'nnTTC
Cuts at: 679 679
Size: 3914
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI