Restriction map for
pF1KB6366
(Circular) (Six-base) MAPSORT of: pF1KB6366.seq Check: 6441 from: 1 to: 4007
pF1KB6366 4007 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 500 1045 500
Size: 545 3462
Acc65I G'GTAC_C
Cuts at: 1023 1023
Size: 4007
AclI AA'CG_TT
Cuts at: 3847 3847
Size: 4007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1832 2264 3011 3279 1832
Size: 432 747 268 2560
Fragments arranged by size:
2560 747 432 268
AflIII A'CryG_T
Cuts at: 1359 2463 3440 1359
Size: 1104 977 1926
Fragments arranged by size:
1926 1104 977
AgeI A'CCGG_T
Cuts at: 2395 2395
Size: 4007
AlwNI CAG_nnn'CTG
Cuts at: 1312 2879 1312
Size: 1567 2440
ApaLI G'TGCA_C
Cuts at: 730 2777 730
Size: 2047 1960
ApoI r'AATT_y
Cuts at: 1011 3360 3616 1011
Size: 2349 256 1402
Fragments arranged by size:
2349 1402 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
AvaI C'yCGr_G
Cuts at: 1027 1027
Size: 4007
BamHI G'GATC_C
Cuts at: 1032 1032
Size: 4007
BanI G'GyrC_C
Cuts at: 550 925 1023 1437 1685 1720 550
Size: 375 98 414 248 35 2837
Fragments arranged by size:
2837 414 375 248 98 35
BanII G_rGCy'C
Cuts at: 626 1021 2051 626
Size: 395 1030 2582
Fragments arranged by size:
2582 1030 395
BbeI G_GCGC'C
Cuts at: 554 1441 1689 554
Size: 887 248 2872
Fragments arranged by size:
2872 887 248
BciVI GTATCCnnnnn_n'
Cuts at: 1905 2666 4007 1905
Size: 761 1341 1905
Fragments arranged by size:
1905 1341 761
BclI T'GATC_A
Cuts at: 114 1527 114
Size: 1413 2594
BglI GCCn_nnn'nGGC
Cuts at: 478 478
Size: 4007
BglII A'GATC_T
Cuts at: 596 1522 3559 596
Size: 926 2037 1044
Fragments arranged by size:
2037 1044 926
BlpI GC'TnA_GC
Cuts at: 1119 1119
Size: 4007
Bme1580I G_kGCm'C
Cuts at: 734 1632 1725 2781 734
Size: 898 93 1056 1960
Fragments arranged by size:
1960 1056 898 93
BmrI ACTGGGnnnn_n'
Cuts at: 1219 1300 1635 3779 1219
Size: 81 335 2144 1447
Fragments arranged by size:
2144 1447 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 900 339
Size: 561 3446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 2062 2554 2852 1183
Size: 879 492 298 2338
Fragments arranged by size:
2338 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3711 3711
Size: 4007
BsaAI yAC'GTr
Cuts at: 1360 1360
Size: 4007
BsaBI GATnn'nnATC
Cuts at: 6 1521 3558 6
Size: 1515 2037 455
Fragments arranged by size:
2037 1515 455
BsaHI Gr'CG_yC
Cuts at: 155 460 551 1438 1686 2388 3884 155
Size: 305 91 887 248 702 1496 278
Fragments arranged by size:
1496 887 702 305 278 248 91
BsaWI w'CCGG_w
Cuts at: 463 1196 1717 2395 2669 2816 3227 463
Size: 733 521 678 274 147 411 1243
Fragments arranged by size:
1243 733 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 475 557 1432 2190 2767 3263 475
Size: 82 875 758 577 496 1219
Fragments arranged by size:
1219 875 758 577 496 82
BsiEI CG_ry'CG
Cuts at: 104 185 837 1549 1595 2803 104
Size: 81 652 712 46 1208 1308
Fragments arranged by size:
1308 1208 712 652 81 46
BsiHKAI G_wGCw'C
Cuts at: 734 1021 1799 1989 2781 734
Size: 287 778 190 792 1960
Fragments arranged by size:
1960 792 778 287 190
BsmBI CGTCTCn'nnnn_
Cuts at: 174 648 174
Size: 474 3533
Bsp1286I G_dGCh'C
Cuts at: 626 734 1021 1632 1725 1799 1989 2051 2781
Size: 108 287 611 93 74 190 62 730
Cuts at: 2781 626
Size: 1852
Fragments arranged by size:
1852 730 611 287 190 108 93 74
62
BspEI T'CCGG_A
Cuts at: 3227 3227
Size: 4007
BspHI T'CATG_A
Cuts at: 3183 3183
Size: 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 207 1057 1271 1573 1954 207
Size: 850 214 302 381 2260
Fragments arranged by size:
2260 850 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2299 2353 3837 4002 50
Size: 2249 54 1484 165 55
Fragments arranged by size:
2249 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1919 1919
Size: 4007
BsrFI r'CCGG_y
Cuts at: 2005 2186 2395 2005
Size: 181 209 3617
Fragments arranged by size:
3617 209 181
BsrGI T'GTAC_A
Cuts at: 857 857
Size: 4007
BssSI C'ACGA_G
Cuts at: 2278 2636 2278
Size: 358 3649
BstAPI GCAn_nnn'nTGC
Cuts at: 1384 1384
Size: 4007
BstBI TT'CG_AA
Cuts at: 2368 2368
Size: 4007
BstEII G'GTnAC_C
Cuts at: 325 451 325
Size: 126 3881
BstYI r'GATC_y
Cuts at: 1 596 1032 1499 1522 1857 2103 3115 3201
Size: 595 436 467 23 335 246 1012 86
Cuts at: 3201 3213 3559 1
Size: 12 346 449
Fragments arranged by size:
1012 595 467 449 436 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 293 616 293
Size: 323 3684
BtgI C'CryG_G
Cuts at: 107 179 570 107
Size: 72 391 3544
Fragments arranged by size:
3544 391 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1957 2138 2301 1957
Size: 181 163 3663
Fragments arranged by size:
3663 181 163
BtsI GCAGTG_nn'
Cuts at: 1250 1250
Size: 4007
DraI TTT'AAA
Cuts at: 1006 1006
Size: 4007
DrdI GACnn_nn'nnGTC
Cuts at: 1713 2571 1713
Size: 858 3149
EaeI y'GGCC_r
Cuts at: 146 332 834 1592 1766 2157 2184 3529 146
Size: 186 502 758 174 391 27 1345 624
Fragments arranged by size:
1345 758 624 502 391 186 174 27
EagI C'GGCC_G
Cuts at: 834 1592 834
Size: 758 3249
EarI CTCTTCn'nnn_
Cuts at: 406 2030 2240 406
Size: 1624 210 2173
Fragments arranged by size:
2173 1624 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2215 2525 2671 3817 2215
Size: 310 146 1146 2405
Fragments arranged by size:
2405 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1019 1019
Size: 4007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 900 1832 2264 3011 3279 339
Size: 561 932 432 747 268 1067
Fragments arranged by size:
1067 932 747 561 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 318 318
Size: 4007
EcoO109I rG'GnC_Cy
Cuts at: 1146 1146
Size: 4007
EcoRI G'AATT_C
Cuts at: 1011 1011
Size: 4007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 417 933 2026 417
Size: 516 1093 2398
Fragments arranged by size:
2398 1093 516
FspI TGC'GCA
Cuts at: 1788 1788
Size: 4007
HaeII r_GCGC'y
Cuts at: 441 554 1441 1689 2711 3690 441
Size: 113 887 248 1022 979 758
Fragments arranged by size:
1022 979 887 758 248 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1849 1879 1881 1911 1849
Size: 30 2 30 3945
Fragments arranged by size:
3945 30 30 2
HincII GTy'rAC
Cuts at: 873 1046 873
Size: 173 3834
KasI G'GCGC_C
Cuts at: 550 1437 1685 550
Size: 887 248 2872
Fragments arranged by size:
2872 887 248
KpnI G_GTAC'C
Cuts at: 1027 1027
Size: 4007
MluI A'CGCG_T
Cuts at: 3440 3440
Size: 4007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2678 2862 2678
Size: 184 3823
MscI TGG'CCA
Cuts at: 334 1768 334
Size: 1434 2573
MslI CAynn'nnrTG
Cuts at: 739 1996 2123 739
Size: 1257 127 2623
Fragments arranged by size:
2623 1257 127
MspA1I CmG'CkG
Cuts at: 163 181 475 507 1118 1432 1792 2805 3050
Size: 18 294 32 611 314 360 1013 245
Cuts at: 3050 163
Size: 1120
Fragments arranged by size:
1120 1013 611 360 314 294 245 32
18
NaeI GCC'GGC
Cuts at: 2188 2188
Size: 4007
NarI GG'CG_CC
Cuts at: 551 1438 1686 551
Size: 887 248 2872
Fragments arranged by size:
2872 887 248
NcoI C'CATG_G
Cuts at: 107 570 107
Size: 463 3544
NgoMIV G'CCGG_C
Cuts at: 2186 2186
Size: 4007
NspI r_CATG'y
Cuts at: 229 1060 1560 2467 229
Size: 831 500 907 1769
Fragments arranged by size:
1769 907 831 500
PciI A'CATG_T
Cuts at: 2463 2463
Size: 4007
PflMI CCAn_nnn'nTGG
Cuts at: 576 576
Size: 4007
PmeI GTTT'AAAC
Cuts at: 1006 1006
Size: 4007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3975
PshAI GACnn'nnGTC
Cuts at: 181 772 181
Size: 591 3416
PstI C_TGCA'G
Cuts at: 218 1054 1739 218
Size: 836 685 2486
Fragments arranged by size:
2486 836 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4007
PvuII CAG'CTG
Cuts at: 1432 1792 1432
Size: 360 3647
RsrII CG'GwC_CG
Cuts at: 2202 2202
Size: 4007
SacI G_AGCT'C
Cuts at: 1021 1021
Size: 4007
SacII CC_GC'GG
Cuts at: 182 182
Size: 4007
SalI G'TCGA_C
Cuts at: 1044 1044
Size: 4007
SapI GCTCTTCn'nnn_
Cuts at: 406 2030 2240 406
Size: 1624 210 2173
Fragments arranged by size:
2173 1624 210
SbfI CC_TGCA'GG
Cuts at: 1054 1054
Size: 4007
ScaI AGT'ACT
Cuts at: 3524 3524
Size: 4007
SexAI A'CCwGG_T
Cuts at: 523 523
Size: 4007
SfcI C'TryA_G
Cuts at: 33 214 666 1050 1735 2728 2919 33
Size: 181 452 384 685 993 191 1121
Fragments arranged by size:
1121 993 685 452 384 191 181
SfiI GGCCn_nnn'nGGCC
Cuts at: 478 478
Size: 4007
SfoI GGC'GCC
Cuts at: 552 1439 1687 552
Size: 887 248 2872
Fragments arranged by size:
2872 887 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
SmaI CCC'GGG
Cuts at: 1029 1029
Size: 4007
SmlI C'TyrA_G
Cuts at: 1162 2077 2569 2831 1162
Size: 915 492 262 2338
Fragments arranged by size:
2338 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4007
SphI G_CATG'C
Cuts at: 229 1060 1560 229
Size: 831 500 2676
Fragments arranged by size:
2676 831 500
StuI AGG'CCT
Cuts at: 913 913
Size: 4007
StyI C'CwwG_G
Cuts at: 107 570 651 701 908 1141 1495 107
Size: 463 81 50 207 233 354 2619
Fragments arranged by size:
2619 463 354 233 207 81 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 373 1590 1723 2182 2391 100
Size: 273 1217 133 459 209 1716
Fragments arranged by size:
1716 1217 459 273 209 133
TatI w'GTAC_w
Cuts at: 257 389 857 3522 257
Size: 132 468 2665 742
Fragments arranged by size:
2665 742 468 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 3975
Tth111I GACn'n_nGTC
Cuts at: 1804 1804
Size: 4007
XbaI T'CTAG_A
Cuts at: 1038 1038
Size: 4007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1502 1502
Size: 4007
XmaI C'CCGG_G
Cuts at: 1027 1027
Size: 4007
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PsiI PspOMI
PspXI PsrI SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI