Restriction map for
pF1KB6367
(Circular) (Six-base) MAPSORT of: pF1KB6367.seq Check: 877 from: 1 to: 3920
pF1KB6367 3920 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 958 958
Size: 3920
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 1745 2177 2924 3192 273
Size: 1472 432 747 268 1001
Fragments arranged by size:
1472 1001 747 432 268
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 157 189 157
Size: 32 3888
AlwNI CAG_nnn'CTG
Cuts at: 222 1225 2792 222
Size: 1003 1567 1350
Fragments arranged by size:
1567 1350 1003
ApaLI G'TGCA_C
Cuts at: 448 2690 448
Size: 2242 1678
ApoI r'AATT_y
Cuts at: 787 924 3273 3529 787
Size: 137 2349 256 1178
Fragments arranged by size:
2349 1178 256 137
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 940 940
Size: 3920
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 936 1350 1598 1633 936
Size: 414 248 35 3223
Fragments arranged by size:
3223 414 248 35
BanII G_rGCy'C
Cuts at: 128 934 1964 128
Size: 806 1030 2084
Fragments arranged by size:
2084 1030 806
BbeI G_GCGC'C
Cuts at: 1354 1602 1354
Size: 248 3672
BbsI GAAGACnn'nnnn_
Cuts at: 747 843 902 747
Size: 96 59 3765
Fragments arranged by size:
3765 96 59
BbvCI CC'TCA_GC
Cuts at: 328 328
Size: 3920
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BglII A'GATC_T
Cuts at: 1435 3472 1435
Size: 2037 1883
BlpI GC'TnA_GC
Cuts at: 1032 1032
Size: 3920
Bme1580I G_kGCm'C
Cuts at: 452 1545 1638 2694 452
Size: 1093 93 1056 1678
Fragments arranged by size:
1678 1093 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 795 1132 1213 1548 3692 795
Size: 337 81 335 2144 1023
Fragments arranged by size:
2144 1023 337 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 3920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 721 532
Size: 189 3731
Bpu10I CC'TnA_GC
Cuts at: 122 328 376 122
Size: 206 48 3666
Fragments arranged by size:
3666 206 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 815 1096 1975 2467 2765 625
Size: 190 281 879 492 298 1780
Fragments arranged by size:
1780 879 492 298 281 190
BsaI GGTCTCn'nnnn_
Cuts at: 3624 3624
Size: 3920
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 769 1434 3471 6
Size: 763 665 2037 455
Fragments arranged by size:
2037 763 665 455
BsaHI Gr'CG_yC
Cuts at: 1351 1599 2301 3797 1351
Size: 248 702 1496 1474
Fragments arranged by size:
1496 1474 702 248
BsaWI w'CCGG_w
Cuts at: 1109 1630 2308 2582 2729 3140 1109
Size: 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1345 2103 2680 3176 1345
Size: 758 577 496 2089
Fragments arranged by size:
2089 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 462
Size: 3920
BsiEI CG_ry'CG
Cuts at: 104 1462 1508 2716 104
Size: 1358 46 1208 1308
Fragments arranged by size:
1358 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 128 211 382 452 934 1712 1902 2694 128
Size: 83 171 70 482 778 190 792 1354
Fragments arranged by size:
1354 792 778 482 190 171 83 70
Bsp1286I G_dGCh'C
Cuts at: 128 211 382 452 934 1545 1638 1712 1902
Size: 83 171 70 482 611 93 74 190
Cuts at: 1902 1964 2694 128
Size: 62 730 1354
Fragments arranged by size:
1354 730 611 482 190 171 93 83
74 70 62
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 970 1184 1486 1867 970
Size: 214 302 381 3023
Fragments arranged by size:
3023 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2212 2266 3750 3915 50
Size: 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 165 1832 165
Size: 1667 2253
BsrFI r'CCGG_y
Cuts at: 1918 2099 2308 1918
Size: 181 209 3530
Fragments arranged by size:
3530 209 181
BsrGI T'GTAC_A
Cuts at: 594 594
Size: 3920
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 1297 1297
Size: 3920
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstYI r'GATC_y
Cuts at: 1 711 945 1412 1435 1770 2016 3028 3114
Size: 710 234 467 23 335 246 1012 86
Cuts at: 3114 3126 3472 1
Size: 12 346 449
Fragments arranged by size:
1012 710 467 449 346 335 246 234
86 23 12
BtgI C'CryG_G
Cuts at: 107 317 107
Size: 210 3710
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1870 2051 2214 1870
Size: 181 163 3576
Fragments arranged by size:
3576 181 163
BtsI GCAGTG_nn'
Cuts at: 1163 1163
Size: 3920
DraI TTT'AAA
Cuts at: 919 919
Size: 3920
DrdI GACnn_nn'nnGTC
Cuts at: 1626 2484 1626
Size: 858 3062
EaeI y'GGCC_r
Cuts at: 314 873 1505 1679 2070 2097 3442 314
Size: 559 632 174 391 27 1345 792
Fragments arranged by size:
1345 792 632 559 391 174 27
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2128 2438 2584 3730 2128
Size: 310 146 1146 2318
Fragments arranged by size:
2318 1146 310 146
EcoICRI GAG'CTC
Cuts at: 126 932 126
Size: 806 3114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 532 721 1745 2177 2924 3192 273
Size: 259 189 1024 432 747 268 1001
Fragments arranged by size:
1024 1001 747 432 268 259 189
EcoO109I rG'GnC_Cy
Cuts at: 219 1059 219
Size: 840 3080
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 228 1939 228
Size: 1711 2209
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 1354 1602 2624 3603 1354
Size: 248 1022 979 1671
Fragments arranged by size:
1671 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 504 536 696 728 1762 1792 1794 1824 504
Size: 32 160 32 1034 30 2 30 2600
Fragments arranged by size:
2600 1034 160 32 32 30 30 2
HincII GTy'rAC
Cuts at: 959 959
Size: 3920
HindIII A'AGCT_T
Cuts at: 504 504
Size: 3920
KasI G'GCGC_C
Cuts at: 1350 1598 1350
Size: 248 3672
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 290 2591 2775 290
Size: 2301 184 1435
Fragments arranged by size:
2301 1435 184
MscI TGG'CCA
Cuts at: 316 1681 316
Size: 1365 2555
MslI CAynn'nnrTG
Cuts at: 1909 2036 1909
Size: 127 3793
MspA1I CmG'CkG
Cuts at: 699 1031 1345 1705 2718 2963 699
Size: 332 314 360 1013 245 1656
Fragments arranged by size:
1656 1013 360 332 314 245
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 3920
NarI GG'CG_CC
Cuts at: 1351 1599 1351
Size: 248 3672
NcoI C'CATG_G
Cuts at: 107 317 107
Size: 210 3710
NdeI CA'TA_TG
Cuts at: 525 525
Size: 3920
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 3920
NspI r_CATG'y
Cuts at: 846 973 1473 2380 846
Size: 127 500 907 2386
Fragments arranged by size:
2386 907 500 127
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PflMI CCAn_nnn'nTGG
Cuts at: 222 222
Size: 3920
PfoI T'CCnGG_A
Cuts at: 215 362 485 215
Size: 147 123 3650
Fragments arranged by size:
3650 147 123
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PpuMI rG'GwC_Cy
Cuts at: 219 219
Size: 3920
PstI C_TGCA'G
Cuts at: 967 1652 967
Size: 685 3235
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 699 1345 1705 699
Size: 646 360 2914
Fragments arranged by size:
2914 646 360
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 128 934 128
Size: 806 3114
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 276 694 963 1648 2641 2832 33
Size: 243 418 269 685 993 191 1121
Fragments arranged by size:
1121 993 685 418 269 243 191
SfoI GGC'GCC
Cuts at: 1352 1600 1352
Size: 248 3672
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 640 830 1075 1990 2482 2744 640
Size: 190 245 915 492 262 1816
Fragments arranged by size:
1816 915 492 262 245 190
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StyI C'CwwG_G
Cuts at: 107 317 632 1054 1408 107
Size: 210 315 422 354 2619
Fragments arranged by size:
2619 422 354 315 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1503 1636 2095 2304 1503
Size: 133 459 209 3119
Fragments arranged by size:
3119 459 209 133
TatI w'GTAC_w
Cuts at: 594 3435 594
Size: 2841 1079
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 344 372 376 404 3437 3469 344
Size: 28 4 28 3033 32 795
Fragments arranged by size:
3033 795 32 28 28 4
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 138 696 1415 138
Size: 558 719 2643
Fragments arranged by size:
2643 719 558
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BseRI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI