Restriction map for
pF1KB6368
(Circular) (Six-base) MAPSORT of: pF1KB6368.seq Check: 8386 from: 1 to: 4007
pF1KB6368 4007 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 297 297
Size: 4007
AccI GT'mk_AC
Cuts at: 587 1045 587
Size: 458 3549
Acc65I G'GTAC_C
Cuts at: 1023 1023
Size: 4007
AclI AA'CG_TT
Cuts at: 3847 3847
Size: 4007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 1832 2264 3011 3279 347
Size: 1485 432 747 268 1075
Fragments arranged by size:
1485 1075 747 432 268
AflIII A'CryG_T
Cuts at: 1359 2463 3440 1359
Size: 1104 977 1926
Fragments arranged by size:
1926 1104 977
AgeI A'CCGG_T
Cuts at: 2395 2395
Size: 4007
AleI CACnn'nnGTG
Cuts at: 496 496
Size: 4007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 184 658 184
Size: 474 3533
AlwNI CAG_nnn'CTG
Cuts at: 1312 2879 1312
Size: 1567 2440
ApaLI G'TGCA_C
Cuts at: 2777 2777
Size: 4007
ApoI r'AATT_y
Cuts at: 766 1011 3360 3616 766
Size: 245 2349 256 1157
Fragments arranged by size:
2349 1157 256 245
AseI AT'TA_AT
Cuts at: 20 20
Size: 4007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
AvaI C'yCGr_G
Cuts at: 1027 1027
Size: 4007
BamHI G'GATC_C
Cuts at: 1032 1032
Size: 4007
BanI G'GyrC_C
Cuts at: 1023 1437 1685 1720 1023
Size: 414 248 35 3310
Fragments arranged by size:
3310 414 248 35
BanII G_rGCy'C
Cuts at: 632 812 1021 2051 632
Size: 180 209 1030 2588
Fragments arranged by size:
2588 1030 209 180
BbeI G_GCGC'C
Cuts at: 1441 1689 1441
Size: 248 3759
BbsI GAAGACnn'nnnn_
Cuts at: 299 391 491 299
Size: 92 100 3815
Fragments arranged by size:
3815 100 92
BciVI GTATCCnnnnn_n'
Cuts at: 1905 2666 4007 1905
Size: 761 1341 1905
Fragments arranged by size:
1905 1341 761
BclI T'GATC_A
Cuts at: 1527 1527
Size: 4007
BglII A'GATC_T
Cuts at: 1522 3559 1522
Size: 2037 1970
BlpI GC'TnA_GC
Cuts at: 1119 1119
Size: 4007
Bme1580I G_kGCm'C
Cuts at: 674 960 1632 1725 2781 674
Size: 286 672 93 1056 1900
Fragments arranged by size:
1900 1056 672 286 93
BmrI ACTGGGnnnn_n'
Cuts at: 1219 1300 1635 3779 1219
Size: 81 335 2144 1447
Fragments arranged by size:
2144 1447 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 639 2374 639
Size: 1735 2272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 561 395
Size: 166 3841
Bpu10I CC'TnA_GC
Cuts at: 205 205
Size: 4007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1183 2062 2554 2852 946
Size: 237 879 492 298 2101
Fragments arranged by size:
2101 879 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 3711 3711
Size: 4007
BsaAI yAC'GTr
Cuts at: 1360 1360
Size: 4007
BsaBI GATnn'nnATC
Cuts at: 6 1521 3558 6
Size: 1515 2037 455
Fragments arranged by size:
2037 1515 455
BsaHI Gr'CG_yC
Cuts at: 1438 1686 2388 3884 1438
Size: 248 702 1496 1561
Fragments arranged by size:
1561 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1196 1717 2395 2669 2816 3227 1196
Size: 521 678 274 147 411 1976
Fragments arranged by size:
1976 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 437 467 437
Size: 30 3977
BseYI C'CCAG_C
Cuts at: 230 243 441 873 902 1432 2190 2767 3263
Size: 13 198 432 29 530 758 577 496
Cuts at: 3263 230
Size: 974
Fragments arranged by size:
974 758 577 530 496 432 198 29
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 130
Size: 4007
BsiEI CG_ry'CG
Cuts at: 104 889 1549 1595 2803 104
Size: 785 660 46 1208 1308
Fragments arranged by size:
1308 1208 785 660 46
BsiHKAI G_wGCw'C
Cuts at: 211 632 812 1001 1021 1799 1989 2781 211
Size: 421 180 189 20 778 190 792 1437
Fragments arranged by size:
1437 792 778 421 190 189 180 20
Bsp1286I G_dGCh'C
Cuts at: 211 632 674 812 960 1001 1021 1632 1725
Size: 421 42 138 148 41 20 611 93
Cuts at: 1725 1799 1989 2051 2781 211
Size: 74 190 62 730 1437
Fragments arranged by size:
1437 730 611 421 190 148 138 93
74 62 42 41 20
BspEI T'CCGG_A
Cuts at: 3227 3227
Size: 4007
BspHI T'CATG_A
Cuts at: 3183 3183
Size: 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 297 1057 1271 1573 1954 297
Size: 760 214 302 381 2350
Fragments arranged by size:
2350 760 381 302 214
BsrBI CCG'CTC
Cuts at: 50 971 2299 2353 3837 4002 50
Size: 921 1328 54 1484 165 55
Fragments arranged by size:
1484 1328 921 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1919 1919
Size: 4007
BsrFI r'CCGG_y
Cuts at: 149 888 2005 2186 2395 149
Size: 739 1117 181 209 1761
Fragments arranged by size:
1761 1117 739 209 181
BssHII G'CGCG_C
Cuts at: 906 906
Size: 4007
BssSI C'ACGA_G
Cuts at: 682 2278 2636 682
Size: 1596 358 2053
Fragments arranged by size:
2053 1596 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1384 1384
Size: 4007
BstBI TT'CG_AA
Cuts at: 2368 2368
Size: 4007
BstXI CCAn_nnnn'nTGG
Cuts at: 196 302 498 196
Size: 106 196 3705
Fragments arranged by size:
3705 196 106
BstYI r'GATC_y
Cuts at: 1 369 1032 1499 1522 1857 2103 3115 3201
Size: 368 663 467 23 335 246 1012 86
Cuts at: 3201 3213 3559 1
Size: 12 346 449
Fragments arranged by size:
1012 663 467 449 368 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 588 588
Size: 4007
BtgI C'CryG_G
Cuts at: 181 181
Size: 4007
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 579 1957 2138 2301 579
Size: 1378 181 163 2285
Fragments arranged by size:
2285 1378 181 163
BtsI GCAGTG_nn'
Cuts at: 166 576 1250 166
Size: 410 674 2923
Fragments arranged by size:
2923 674 410
DraI TTT'AAA
Cuts at: 1006 1006
Size: 4007
DraIII CAC_nnn'GTG
Cuts at: 801 801
Size: 4007
DrdI GACnn_nn'nnGTC
Cuts at: 1713 2571 1713
Size: 858 3149
EaeI y'GGCC_r
Cuts at: 198 395 803 860 886 890 992 1592 1766
Size: 197 408 57 26 4 102 600 174
Cuts at: 1766 2157 2184 3529 198
Size: 391 27 1345 676
Fragments arranged by size:
1345 676 600 408 391 197 174 102
57 27 26 4
EagI C'GGCC_G
Cuts at: 886 1592 886
Size: 706 3301
EarI CTCTTCn'nnn_
Cuts at: 725 817 2030 2240 725
Size: 92 1213 210 2492
Fragments arranged by size:
2492 1213 210 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 771 2215 2525 2671 3817 771
Size: 1444 310 146 1146 961
Fragments arranged by size:
1444 1146 961 310 146
EcoICRI GAG'CTC
Cuts at: 630 810 1019 630
Size: 180 209 3618
Fragments arranged by size:
3618 209 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 395 561 1832 2264 3011 3279 347
Size: 48 166 1271 432 747 268 1075
Fragments arranged by size:
1271 1075 747 432 268 166 48
EcoO109I rG'GnC_Cy
Cuts at: 426 464 1146 426
Size: 38 682 3287
Fragments arranged by size:
3287 682 38
EcoRI G'AATT_C
Cuts at: 1011 1011
Size: 4007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 408 717 2026 408
Size: 309 1309 2389
Fragments arranged by size:
2389 1309 309
FseI GG_CCGG'CC
Cuts at: 892 892
Size: 4007
FspI TGC'GCA
Cuts at: 1788 1788
Size: 4007
HaeII r_GCGC'y
Cuts at: 1441 1689 2711 3690 1441
Size: 248 1022 979 1758
Fragments arranged by size:
1758 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 1849 1879 1881 1911 289
Size: 32 1528 30 2 30 2385
Fragments arranged by size:
2385 1528 32 30 30 2
HincII GTy'rAC
Cuts at: 1046 1046
Size: 4007
KasI G'GCGC_C
Cuts at: 1437 1685 1437
Size: 248 3759
KpnI G_GTAC'C
Cuts at: 1027 1027
Size: 4007
MluI A'CGCG_T
Cuts at: 3440 3440
Size: 4007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2678 2862 2678
Size: 184 3823
MscI TGG'CCA
Cuts at: 397 1768 397
Size: 1371 2636
MslI CAynn'nnrTG
Cuts at: 496 682 1996 2123 496
Size: 186 1314 127 2380
Fragments arranged by size:
2380 1314 186 127
MspA1I CmG'CkG
Cuts at: 441 642 710 1118 1432 1792 2805 3050 441
Size: 201 68 408 314 360 1013 245 1398
Fragments arranged by size:
1398 1013 408 360 314 245 201 68
NaeI GCC'GGC
Cuts at: 890 2188 890
Size: 1298 2709
NarI GG'CG_CC
Cuts at: 1438 1686 1438
Size: 248 3759
NgoMIV G'CCGG_C
Cuts at: 888 2186 888
Size: 1298 2709
NspI r_CATG'y
Cuts at: 1060 1560 2467 1060
Size: 500 907 2600
Fragments arranged by size:
2600 907 500
PciI A'CATG_T
Cuts at: 2463 2463
Size: 4007
PflMI CCAn_nnn'nTGG
Cuts at: 990 990
Size: 4007
PfoI T'CCnGG_A
Cuts at: 372 372
Size: 4007
PmeI GTTT'AAAC
Cuts at: 1006 1006
Size: 4007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3975
PpuMI rG'GwC_Cy
Cuts at: 426 464 426
Size: 38 3969
PstI C_TGCA'G
Cuts at: 563 794 1054 1739 563
Size: 231 260 685 2831
Fragments arranged by size:
2831 685 260 231
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4007
PvuII CAG'CTG
Cuts at: 441 642 1432 1792 441
Size: 201 790 360 2656
Fragments arranged by size:
2656 790 360 201
RsrII CG'GwC_CG
Cuts at: 2202 2202
Size: 4007
SacI G_AGCT'C
Cuts at: 632 812 1021 632
Size: 180 209 3618
Fragments arranged by size:
3618 209 180
SalI G'TCGA_C
Cuts at: 1044 1044
Size: 4007
SapI GCTCTTCn'nnn_
Cuts at: 817 2030 2240 817
Size: 1213 210 2584
Fragments arranged by size:
2584 1213 210
SbfI CC_TGCA'GG
Cuts at: 563 1054 563
Size: 491 3516
ScaI AGT'ACT
Cuts at: 3524 3524
Size: 4007
SfcI C'TryA_G
Cuts at: 33 559 790 1050 1735 2728 2919 33
Size: 526 231 260 685 993 191 1121
Fragments arranged by size:
1121 993 685 526 260 231 191
SfoI GGC'GCC
Cuts at: 1439 1687 1439
Size: 248 3759
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
SgrAI Cr'CCGG_yG
Cuts at: 149 149
Size: 4007
SmaI CCC'GGG
Cuts at: 1029 1029
Size: 4007
SmlI C'TyrA_G
Cuts at: 961 1162 2077 2569 2831 961
Size: 201 915 492 262 2137
Fragments arranged by size:
2137 915 492 262 201
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4007
SphI G_CATG'C
Cuts at: 1060 1560 1060
Size: 500 3507
StyI C'CwwG_G
Cuts at: 863 1141 1495 863
Size: 278 354 3375
Fragments arranged by size:
3375 354 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 289 617 1590 1723 2182 2391 289
Size: 328 973 133 459 209 1905
Fragments arranged by size:
1905 973 459 328 209 133
TatI w'GTAC_w
Cuts at: 3522 3522
Size: 4007
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 3975
Tth111I GACn'n_nGTC
Cuts at: 1804 1804
Size: 4007
XbaI T'CTAG_A
Cuts at: 1038 1038
Size: 4007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 681 1502 681
Size: 821 3186
XmaI C'CCGG_G
Cuts at: 1027 1027
Size: 4007
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BseRI
BsiWI BsmI BsmBI BsrGI BstEII Bsu36I ClaI CspCI
EcoNI EcoRV FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI