Restriction map for
pF1KB6369
(Circular) (Six-base) MAPSORT of: pF1KB6369.seq Check: 8533 from: 1 to: 3920
pF1KB6369 3920 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 958 958
Size: 3920
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 383 1745 2177 2924 3192 188
Size: 195 1362 432 747 268 916
Fragments arranged by size:
1362 916 747 432 268 195
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AlwNI CAG_nnn'CTG
Cuts at: 1225 2792 1225
Size: 1567 2353
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 924 3273 3529 924
Size: 2349 256 1315
Fragments arranged by size:
2349 1315 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 940 940
Size: 3920
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 936 1350 1598 1633 936
Size: 414 248 35 3223
Fragments arranged by size:
3223 414 248 35
BanII G_rGCy'C
Cuts at: 934 1964 934
Size: 1030 2890
BbeI G_GCGC'C
Cuts at: 1354 1602 1354
Size: 248 3672
BbsI GAAGACnn'nnnn_
Cuts at: 371 473 668 371
Size: 102 195 3623
Fragments arranged by size:
3623 195 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 250 284 250
Size: 34 3886
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BglII A'GATC_T
Cuts at: 414 1435 3472 414
Size: 1021 2037 862
Fragments arranged by size:
2037 1021 862
BlpI GC'TnA_GC
Cuts at: 272 1032 272
Size: 760 3160
Bme1580I G_kGCm'C
Cuts at: 1545 1638 2694 1545
Size: 93 1056 2771
Fragments arranged by size:
2771 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1132 1213 1548 3692 1132
Size: 81 335 2144 1360
Fragments arranged by size:
2144 1360 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 3920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 657 885 1096 1975 2467 2765 657
Size: 228 211 879 492 298 1812
Fragments arranged by size:
1812 879 492 298 228 211
BsaI GGTCTCn'nnnn_
Cuts at: 3624 3624
Size: 3920
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 125 1434 3471 6
Size: 119 1309 2037 455
Fragments arranged by size:
2037 1309 455 119
BsaHI Gr'CG_yC
Cuts at: 1351 1599 2301 3797 1351
Size: 248 702 1496 1474
Fragments arranged by size:
1496 1474 702 248
BsaWI w'CCGG_w
Cuts at: 129 1109 1630 2308 2582 2729 3140 129
Size: 980 521 678 274 147 411 909
Fragments arranged by size:
980 909 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1345 2103 2680 3176 1345
Size: 758 577 496 2089
Fragments arranged by size:
2089 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1462 1508 2716 104
Size: 1358 46 1208 1308
Fragments arranged by size:
1358 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 934 1712 1902 2694 934
Size: 778 190 792 2160
Fragments arranged by size:
2160 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 934 1545 1638 1712 1902 1964 2694 934
Size: 611 93 74 190 62 730 2160
Fragments arranged by size:
2160 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 129 3140 129
Size: 3011 909
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 970 1184 1486 1867 970
Size: 214 302 381 3023
Fragments arranged by size:
3023 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2212 2266 3750 3915 50
Size: 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1832 1832
Size: 3920
BsrFI r'CCGG_y
Cuts at: 1918 2099 2308 1918
Size: 181 209 3530
Fragments arranged by size:
3530 209 181
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 1297 1297
Size: 3920
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstYI r'GATC_y
Cuts at: 1 120 126 414 879 945 1412 1435 1770
Size: 119 6 288 465 66 467 23 335
Cuts at: 1770 2016 3028 3114 3126 3472 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 465 449 346 335 288 246
119 86 66 23 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1870 2051 2214 1870
Size: 181 163 3576
Fragments arranged by size:
3576 181 163
BtsI GCAGTG_nn'
Cuts at: 739 1163 739
Size: 424 3496
DraI TTT'AAA
Cuts at: 919 919
Size: 3920
DrdI GACnn_nn'nnGTC
Cuts at: 1626 2484 1626
Size: 858 3062
EaeI y'GGCC_r
Cuts at: 823 1505 1679 2070 2097 3442 823
Size: 682 174 391 27 1345 1301
Fragments arranged by size:
1345 1301 682 391 174 27
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 386 1943 2153 386
Size: 1557 210 2153
Fragments arranged by size:
2153 1557 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2128 2438 2584 3730 2128
Size: 310 146 1146 2318
Fragments arranged by size:
2318 1146 310 146
EcoICRI GAG'CTC
Cuts at: 932 932
Size: 3920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 383 1745 2177 2924 3192 188
Size: 195 1362 432 747 268 916
Fragments arranged by size:
1362 916 747 432 268 195
EcoO109I rG'GnC_Cy
Cuts at: 1059 1059
Size: 3920
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 193 486 1939 193
Size: 293 1453 2174
Fragments arranged by size:
2174 1453 293
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 1354 1602 2624 3603 1354
Size: 248 1022 979 1671
Fragments arranged by size:
1671 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 399 431 1762 1792 1794 1824 399
Size: 32 1331 30 2 30 2495
Fragments arranged by size:
2495 1331 32 30 30 2
HincII GTy'rAC
Cuts at: 959 959
Size: 3920
HindIII A'AGCT_T
Cuts at: 902 902
Size: 3920
KasI G'GCGC_C
Cuts at: 1350 1598 1350
Size: 248 3672
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 473 907 2591 2775 473
Size: 434 1684 184 1618
Fragments arranged by size:
1684 1618 434 184
MscI TGG'CCA
Cuts at: 825 1681 825
Size: 856 3064
MslI CAynn'nnrTG
Cuts at: 1909 2036 1909
Size: 127 3793
MspA1I CmG'CkG
Cuts at: 313 1031 1345 1705 2718 2963 313
Size: 718 314 360 1013 245 1270
Fragments arranged by size:
1270 1013 718 360 314 245
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 3920
NarI GG'CG_CC
Cuts at: 1351 1599 1351
Size: 248 3672
NdeI CA'TA_TG
Cuts at: 798 798
Size: 3920
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 3920
NspI r_CATG'y
Cuts at: 973 1473 2380 973
Size: 500 907 2513
Fragments arranged by size:
2513 907 500
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PsiI TTA'TAA
Cuts at: 766 766
Size: 3920
PstI C_TGCA'G
Cuts at: 967 1652 967
Size: 685 3235
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 1345 1705 1345
Size: 360 3560
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 934 934
Size: 3920
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 138 3437 138
Size: 3299 621
SfcI C'TryA_G
Cuts at: 33 140 963 1648 2641 2832 33
Size: 107 823 685 993 191 1121
Fragments arranged by size:
1121 993 823 685 191 107
SfoI GGC'GCC
Cuts at: 1352 1600 1352
Size: 248 3672
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 636 864 1075 1990 2482 2744 636
Size: 228 211 915 492 262 1812
Fragments arranged by size:
1812 915 492 262 228 211
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StyI C'CwwG_G
Cuts at: 644 1054 1408 644
Size: 410 354 3156
Fragments arranged by size:
3156 410 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1503 1636 2095 2304 1503
Size: 133 459 209 3119
Fragments arranged by size:
3119 459 209 133
TatI w'GTAC_w
Cuts at: 136 528 3435 136
Size: 392 2907 621
Fragments arranged by size:
2907 621 392
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 472 504 3437 3469 472
Size: 32 2933 32 923
Fragments arranged by size:
2933 923 32 32
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 123 657 951 123
Size: 534 294 3092
Fragments arranged by size:
3092 534 294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1415 1415
Size: 3920
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
XmnI GAAnn'nnTTC
Cuts at: 350 368 350
Size: 18 3902
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI