Restriction map for
pF1KB6370
(Circular) (Six-base) MAPSORT of: pF1KB6370.seq Check: 9498 from: 1 to: 4007
pF1KB6370 4007 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AatII G_ACGT'C
Cuts at: 239 239
Size: 4007
AccI GT'mk_AC
Cuts at: 626 1045 626
Size: 419 3588
Acc65I G'GTAC_C
Cuts at: 1023 1023
Size: 4007
AclI AA'CG_TT
Cuts at: 3847 3847
Size: 4007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 796 1832 2264 3011 3279 358
Size: 438 1036 432 747 268 1086
Fragments arranged by size:
1086 1036 747 438 432 268
AflIII A'CryG_T
Cuts at: 1359 2463 3440 1359
Size: 1104 977 1926
Fragments arranged by size:
1926 1104 977
AgeI A'CCGG_T
Cuts at: 2395 2395
Size: 4007
AhdI GACnn_n'nnGTC
Cuts at: 306 306
Size: 4007
AlwNI CAG_nnn'CTG
Cuts at: 1312 2879 1312
Size: 1567 2440
ApaLI G'TGCA_C
Cuts at: 817 2777 817
Size: 1960 2047
ApoI r'AATT_y
Cuts at: 544 1011 3360 3616 544
Size: 467 2349 256 935
Fragments arranged by size:
2349 935 467 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
AvaI C'yCGr_G
Cuts at: 827 1027 827
Size: 200 3807
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 279 312 279
Size: 33 3974
BamHI G'GATC_C
Cuts at: 1032 1032
Size: 4007
BanI G'GyrC_C
Cuts at: 120 984 1023 1437 1685 1720 120
Size: 864 39 414 248 35 2407
Fragments arranged by size:
2407 864 414 248 39 35
BanII G_rGCy'C
Cuts at: 1021 2051 1021
Size: 1030 2977
BbeI G_GCGC'C
Cuts at: 1441 1689 1441
Size: 248 3759
BbsI GAAGACnn'nnnn_
Cuts at: 239 239
Size: 4007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 808 842 808
Size: 34 3973
BciVI GTATCCnnnnn_n'
Cuts at: 1905 2666 4007 1905
Size: 761 1341 1905
Fragments arranged by size:
1905 1341 761
BclI T'GATC_A
Cuts at: 1527 1527
Size: 4007
BglII A'GATC_T
Cuts at: 1522 3559 1522
Size: 2037 1970
BlpI GC'TnA_GC
Cuts at: 1119 1119
Size: 4007
Bme1580I G_kGCm'C
Cuts at: 667 821 920 989 1632 1725 2781 667
Size: 154 99 69 643 93 1056 1893
Fragments arranged by size:
1893 1056 643 154 99 93 69
BmrI ACTGGGnnnn_n'
Cuts at: 221 1219 1300 1635 3779 221
Size: 998 81 335 2144 449
Fragments arranged by size:
2144 998 449 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 348 673 259
Size: 89 325 3593
Fragments arranged by size:
3593 325 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 2062 2554 2852 1183
Size: 879 492 298 2338
Fragments arranged by size:
2338 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 397 452 3711 397
Size: 55 3259 693
Fragments arranged by size:
3259 693 55
BsaAI yAC'GTr
Cuts at: 453 630 951 1360 453
Size: 177 321 409 3100
Fragments arranged by size:
3100 409 321 177
BsaBI GATnn'nnATC
Cuts at: 6 1521 3558 6
Size: 1515 2037 455
Fragments arranged by size:
2037 1515 455
BsaHI Gr'CG_yC
Cuts at: 236 1438 1686 2388 3884 236
Size: 1202 248 702 1496 359
Fragments arranged by size:
1496 1202 702 359 248
BsaWI w'CCGG_w
Cuts at: 1196 1717 2395 2669 2816 3227 1196
Size: 521 678 274 147 411 1976
Fragments arranged by size:
1976 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 838 868 646
Size: 30 162 30 3785
Fragments arranged by size:
3785 162 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 670 673 670
Size: 3 4004
BseYI C'CCAG_C
Cuts at: 561 582 971 1432 2190 2767 3263 561
Size: 21 389 461 758 577 496 1305
Fragments arranged by size:
1305 758 577 496 461 389 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 862 973 652
Size: 210 111 3686
Fragments arranged by size:
3686 210 111
BsiEI CG_ry'CG
Cuts at: 104 1549 1595 2803 104
Size: 1445 46 1208 1308
Fragments arranged by size:
1445 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 821 1021 1799 1989 2781 821
Size: 200 778 190 792 2047
Fragments arranged by size:
2047 792 778 200 190
BsmI GAATG_Cn'
Cuts at: 505 505
Size: 4007
Bsp1286I G_dGCh'C
Cuts at: 667 821 920 989 1021 1632 1725 1799 1989
Size: 154 99 69 32 611 93 74 190
Cuts at: 1989 2051 2781 667
Size: 62 730 1893
Fragments arranged by size:
1893 730 611 190 154 99 93 74
69 62 32
BspEI T'CCGG_A
Cuts at: 3227 3227
Size: 4007
BspHI T'CATG_A
Cuts at: 3183 3183
Size: 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1057 1271 1573 1954 1057
Size: 214 302 381 3110
Fragments arranged by size:
3110 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2299 2353 3837 4002 50
Size: 2249 54 1484 165 55
Fragments arranged by size:
2249 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1919 1919
Size: 4007
BsrFI r'CCGG_y
Cuts at: 713 832 2005 2186 2395 713
Size: 119 1173 181 209 2325
Fragments arranged by size:
2325 1173 209 181 119
BssSI C'ACGA_G
Cuts at: 979 2278 2636 979
Size: 1299 358 2350
Fragments arranged by size:
2350 1299 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1384 1384
Size: 4007
BstBI TT'CG_AA
Cuts at: 2368 2368
Size: 4007
BstEII G'GTnAC_C
Cuts at: 738 738
Size: 4007
BstYI r'GATC_y
Cuts at: 1 720 1032 1499 1522 1857 2103 3115 3201
Size: 719 312 467 23 335 246 1012 86
Cuts at: 3201 3213 3559 1
Size: 12 346 449
Fragments arranged by size:
1012 719 467 449 346 335 312 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 317 107
Size: 210 3797
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1957 2138 2301 1957
Size: 181 163 3663
Fragments arranged by size:
3663 181 163
BtsI GCAGTG_nn'
Cuts at: 920 1250 920
Size: 330 3677
DraI TTT'AAA
Cuts at: 1006 1006
Size: 4007
DrdI GACnn_nn'nnGTC
Cuts at: 1713 2571 1713
Size: 858 3149
EaeI y'GGCC_r
Cuts at: 1592 1766 2157 2184 3529 1592
Size: 174 391 27 1345 2070
Fragments arranged by size:
2070 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1592 1592
Size: 4007
EarI CTCTTCn'nnn_
Cuts at: 2030 2240 2030
Size: 210 3797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2215 2525 2671 3817 2215
Size: 310 146 1146 2405
Fragments arranged by size:
2405 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1019 1019
Size: 4007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 348 358 673 796 1832 2264 3011 3279
Size: 89 10 315 123 1036 432 747 268
Cuts at: 3279 259
Size: 987
Fragments arranged by size:
1036 987 747 432 315 268 123 89
10
EcoNI CCTnn'n_nnAGG
Cuts at: 655 655
Size: 4007
EcoO109I rG'GnC_Cy
Cuts at: 154 252 570 753 1146 154
Size: 98 318 183 393 3015
Fragments arranged by size:
3015 393 318 183 98
EcoRI G'AATT_C
Cuts at: 544 1011 544
Size: 467 3540
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2026 2026
Size: 4007
FspI TGC'GCA
Cuts at: 1788 1788
Size: 4007
HaeII r_GCGC'y
Cuts at: 1441 1689 2711 3690 1441
Size: 248 1022 979 1758
Fragments arranged by size:
1758 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 292 324 712 744 1849 1879 1881 1911 292
Size: 32 388 32 1105 30 2 30 2388
Fragments arranged by size:
2388 1105 388 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1046 1046
Size: 4007
KasI G'GCGC_C
Cuts at: 1437 1685 1437
Size: 248 3759
KpnI G_GTAC'C
Cuts at: 1027 1027
Size: 4007
MluI A'CGCG_T
Cuts at: 3440 3440
Size: 4007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2678 2862 2678
Size: 184 3823
MscI TGG'CCA
Cuts at: 1768 1768
Size: 4007
MslI CAynn'nnrTG
Cuts at: 397 1996 2123 397
Size: 1599 127 2281
Fragments arranged by size:
2281 1599 127
MspA1I CmG'CkG
Cuts at: 500 636 1118 1432 1792 2805 3050 500
Size: 136 482 314 360 1013 245 1457
Fragments arranged by size:
1457 1013 482 360 314 245 136
NaeI GCC'GGC
Cuts at: 834 2188 834
Size: 1354 2653
NarI GG'CG_CC
Cuts at: 1438 1686 1438
Size: 248 3759
NcoI C'CATG_G
Cuts at: 107 317 107
Size: 210 3797
NgoMIV G'CCGG_C
Cuts at: 832 2186 832
Size: 1354 2653
NspI r_CATG'y
Cuts at: 229 1060 1560 2467 229
Size: 831 500 907 1769
Fragments arranged by size:
1769 907 831 500
PasI CC'CwG_GG
Cuts at: 802 989 802
Size: 187 3820
PciI A'CATG_T
Cuts at: 2463 2463
Size: 4007
PfoI T'CCnGG_A
Cuts at: 707 707
Size: 4007
PmeI GTTT'AAAC
Cuts at: 1006 1006
Size: 4007
PmlI CAC'GTG
Cuts at: 951 951
Size: 4007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3975
PpuMI rG'GwC_Cy
Cuts at: 154 570 154
Size: 416 3591
PstI C_TGCA'G
Cuts at: 1054 1739 1054
Size: 685 3322
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4007
PvuII CAG'CTG
Cuts at: 1432 1792 1432
Size: 360 3647
RsrII CG'GwC_CG
Cuts at: 2202 2202
Size: 4007
SacI G_AGCT'C
Cuts at: 1021 1021
Size: 4007
SalI G'TCGA_C
Cuts at: 1044 1044
Size: 4007
SanDI GG'GwC_CC
Cuts at: 570 570
Size: 4007
SapI GCTCTTCn'nnn_
Cuts at: 2030 2240 2030
Size: 210 3797
SbfI CC_TGCA'GG
Cuts at: 1054 1054
Size: 4007
ScaI AGT'ACT
Cuts at: 3524 3524
Size: 4007
SfcI C'TryA_G
Cuts at: 33 1050 1735 2728 2919 33
Size: 1017 685 993 191 1121
Fragments arranged by size:
1121 1017 993 685 191
SfoI GGC'GCC
Cuts at: 1439 1687 1439
Size: 248 3759
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
SmaI CCC'GGG
Cuts at: 1029 1029
Size: 4007
SmlI C'TyrA_G
Cuts at: 1162 2077 2569 2831 1162
Size: 915 492 262 2338
Fragments arranged by size:
2338 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4007
SphI G_CATG'C
Cuts at: 1060 1560 1060
Size: 500 3507
SspI AAT'ATT
Cuts at: 517 517
Size: 4007
StyI C'CwwG_G
Cuts at: 107 255 317 641 1141 1495 107
Size: 148 62 324 500 354 2619
Fragments arranged by size:
2619 500 354 324 148 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 891 1590 1723 2182 2391 891
Size: 699 133 459 209 2507
Fragments arranged by size:
2507 699 459 209 133
TatI w'GTAC_w
Cuts at: 3522 3522
Size: 4007
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 3524 3556 646
Size: 32 2846 32 1097
Fragments arranged by size:
2846 1097 32 32
Tth111I GACn'n_nGTC
Cuts at: 1804 1804
Size: 4007
XbaI T'CTAG_A
Cuts at: 1038 1038
Size: 4007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 648 1502 114
Size: 534 854 2619
Fragments arranged by size:
2619 854 534
XmaI C'CCGG_G
Cuts at: 1027 1027
Size: 4007
ZraI GAC'GTC
Cuts at: 237 237
Size: 4007
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI