Restriction map for
pF1KB6371
(Circular) (Six-base) MAPSORT of: pF1KB6371.seq Check: 9385 from: 1 to: 3920
pF1KB6371 3920 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 958 958
Size: 3920
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1745 2177 2924 3192 1745
Size: 432 747 268 2473
Fragments arranged by size:
2473 747 432 268
AflII C'TTAA_G
Cuts at: 708 708
Size: 3920
AflIII A'CryG_T
Cuts at: 912 1272 2376 3353 912
Size: 360 1104 977 1479
Fragments arranged by size:
1479 1104 977 360
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AlwNI CAG_nnn'CTG
Cuts at: 738 1225 2792 738
Size: 487 1567 1866
Fragments arranged by size:
1866 1567 487
ApaI G_GGCC'C
Cuts at: 550 550
Size: 3920
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 311 658 924 3273 3529 311
Size: 347 266 2349 256 702
Fragments arranged by size:
2349 702 347 266 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 940 940
Size: 3920
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 936 1350 1598 1633 936
Size: 414 248 35 3223
Fragments arranged by size:
3223 414 248 35
BanII G_rGCy'C
Cuts at: 169 550 615 934 1964 169
Size: 381 65 319 1030 2125
Fragments arranged by size:
2125 1030 381 319 65
BbeI G_GCGC'C
Cuts at: 1354 1602 1354
Size: 248 3672
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3886
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BglI GCCn_nnn'nGGC
Cuts at: 173 173
Size: 3920
BglII A'GATC_T
Cuts at: 1435 3472 1435
Size: 2037 1883
BlpI GC'TnA_GC
Cuts at: 115 1032 115
Size: 917 3003
Bme1580I G_kGCm'C
Cuts at: 550 1545 1638 2694 550
Size: 995 93 1056 1776
Fragments arranged by size:
1776 1056 995 93
BmrI ACTGGGnnnn_n'
Cuts at: 1132 1213 1548 3692 1132
Size: 81 335 2144 1360
Fragments arranged by size:
2144 1360 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 3920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1975 2467 2765 1096
Size: 879 492 298 2251
Fragments arranged by size:
2251 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 495 523 3624 495
Size: 28 3101 791
Fragments arranged by size:
3101 791 28
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 1434 3471 6
Size: 1428 2037 455
Fragments arranged by size:
2037 1428 455
BsaHI Gr'CG_yC
Cuts at: 1351 1599 2301 3797 1351
Size: 248 702 1496 1474
Fragments arranged by size:
1496 1474 702 248
BsaWI w'CCGG_w
Cuts at: 1109 1630 2308 2582 2729 3140 1109
Size: 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1345 2103 2680 3176 1345
Size: 758 577 496 2089
Fragments arranged by size:
2089 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1462 1508 2716 104
Size: 1358 46 1208 1308
Fragments arranged by size:
1358 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 934 1712 1902 2694 934
Size: 778 190 792 2160
Fragments arranged by size:
2160 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 709 709
Size: 3920
Bsp1286I G_dGCh'C
Cuts at: 169 550 615 934 1545 1638 1712 1902 1964
Size: 381 65 319 611 93 74 190 62
Cuts at: 1964 2694 169
Size: 730 1395
Fragments arranged by size:
1395 730 611 381 319 190 93 74
65 62
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 879 891 970 1184 1486 1867 879
Size: 12 79 214 302 381 2932
Fragments arranged by size:
2932 381 302 214 79 12
BsrBI CCG'CTC
Cuts at: 50 2212 2266 3750 3915 50
Size: 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 206 1832 206
Size: 1626 2294
BsrFI r'CCGG_y
Cuts at: 1918 2099 2308 1918
Size: 181 209 3530
Fragments arranged by size:
3530 209 181
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 862 1297 862
Size: 435 3485
BstBI TT'CG_AA
Cuts at: 132 2281 132
Size: 2149 1771
BstYI r'GATC_y
Cuts at: 1 945 1412 1435 1770 2016 3028 3114 3126
Size: 944 467 23 335 246 1012 86 12
Cuts at: 3126 3472 1
Size: 346 449
Fragments arranged by size:
1012 944 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1870 2051 2214 1870
Size: 181 163 3576
Fragments arranged by size:
3576 181 163
BtsI GCAGTG_nn'
Cuts at: 430 1163 430
Size: 733 3187
DraI TTT'AAA
Cuts at: 677 919 677
Size: 242 3678
DrdI GACnn_nn'nnGTC
Cuts at: 151 1626 2484 151
Size: 1475 858 1587
Fragments arranged by size:
1587 1475 858
EaeI y'GGCC_r
Cuts at: 495 1505 1679 2070 2097 3442 495
Size: 1010 174 391 27 1345 973
Fragments arranged by size:
1345 1010 973 391 174 27
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2128 2438 2584 3730 2128
Size: 310 146 1146 2318
Fragments arranged by size:
2318 1146 310 146
EcoICRI GAG'CTC
Cuts at: 932 932
Size: 3920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1745 2177 2924 3192 1745
Size: 432 747 268 2473
Fragments arranged by size:
2473 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 547 735 1059 547
Size: 188 324 3408
Fragments arranged by size:
3408 324 188
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3920
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 1354 1602 2624 3603 1354
Size: 248 1022 979 1671
Fragments arranged by size:
1671 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 796 828 1762 1792 1794 1824 796
Size: 32 934 30 2 30 2892
Fragments arranged by size:
2892 934 32 30 30 2
HincII GTy'rAC
Cuts at: 324 959 324
Size: 635 3285
KasI G'GCGC_C
Cuts at: 1350 1598 1350
Size: 248 3672
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MfeI C'AATT_G
Cuts at: 344 344
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2591 2775 2591
Size: 184 3736
MscI TGG'CCA
Cuts at: 1681 1681
Size: 3920
MslI CAynn'nnrTG
Cuts at: 1909 2036 1909
Size: 127 3793
MspA1I CmG'CkG
Cuts at: 1031 1345 1705 2718 2963 1031
Size: 314 360 1013 245 1988
Fragments arranged by size:
1988 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 3920
NarI GG'CG_CC
Cuts at: 1351 1599 1351
Size: 248 3672
NdeI CA'TA_TG
Cuts at: 507 650 507
Size: 143 3777
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 3920
NspI r_CATG'y
Cuts at: 916 973 1473 2380 916
Size: 57 500 907 2456
Fragments arranged by size:
2456 907 500 57
PasI CC'CwG_GG
Cuts at: 171 171
Size: 3920
PciI A'CATG_T
Cuts at: 912 2376 912
Size: 1464 2456
PfoI T'CCnGG_A
Cuts at: 821 821
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PpuMI rG'GwC_Cy
Cuts at: 735 735
Size: 3920
PshAI GACnn'nnGTC
Cuts at: 651 651
Size: 3920
PspOMI G'GGCC_C
Cuts at: 546 546
Size: 3920
PstI C_TGCA'G
Cuts at: 595 729 888 967 1652 595
Size: 134 159 79 685 2863
Fragments arranged by size:
2863 685 159 134 79
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 1345 1705 1345
Size: 360 3560
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 934 934
Size: 3920
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 591 725 884 963 1648 2641 2832 33
Size: 558 134 159 79 685 993 191 1121
Fragments arranged by size:
1121 993 685 558 191 159 134 79
SfoI GGC'GCC
Cuts at: 1352 1600 1352
Size: 248 3672
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 708 1075 1990 2482 2744 708
Size: 367 915 492 262 1884
Fragments arranged by size:
1884 915 492 367 262
SpeI A'CTAG_T
Cuts at: 67 684 67
Size: 617 3303
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StyI C'CwwG_G
Cuts at: 1054 1408 1054
Size: 354 3566
SwaI ATTT'AAAT
Cuts at: 677 677
Size: 3920
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1503 1636 2095 2304 1503
Size: 133 459 209 3119
Fragments arranged by size:
3119 459 209 133
TatI w'GTAC_w
Cuts at: 3435 3435
Size: 3920
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 3888
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1415 1415
Size: 3920
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NheI
NotI NruI NsiI PacI PflMI PmlI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI XhoI XmnI ZraI