Restriction map for
pF1KB6373
(Circular) (Six-base) MAPSORT of: pF1KB6373.seq Check: 6439 from: 1 to: 3923
pF1KB6373 3923 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 961 961
Size: 3923
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 2180 2927 3195 1748
Size: 432 747 268 2476
Fragments arranged by size:
2476 747 432 268
AflIII A'CryG_T
Cuts at: 1275 2379 3356 1275
Size: 1104 977 1842
Fragments arranged by size:
1842 1104 977
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AleI CACnn'nnGTG
Cuts at: 892 892
Size: 3923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 850 850
Size: 3923
AlwNI CAG_nnn'CTG
Cuts at: 438 750 1228 2795 438
Size: 312 478 1567 1566
Fragments arranged by size:
1567 1566 478 312
ApaI G_GGCC'C
Cuts at: 210 252 210
Size: 42 3881
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 927 3276 3532 927
Size: 2349 256 1318
Fragments arranged by size:
2349 1318 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 209 943 209
Size: 734 3189
AvrII C'CTAG_G
Cuts at: 712 712
Size: 3923
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 939 1353 1601 1636 939
Size: 414 248 35 3226
Fragments arranged by size:
3226 414 248 35
BanII G_rGCy'C
Cuts at: 210 252 266 937 1967 210
Size: 42 14 671 1030 2166
Fragments arranged by size:
2166 1030 671 42 14
BbeI G_GCGC'C
Cuts at: 1357 1605 1357
Size: 248 3675
BbsI GAAGACnn'nnnn_
Cuts at: 322 322
Size: 3923
BbvCI CC'TCA_GC
Cuts at: 773 773
Size: 3923
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2582 3923 1821
Size: 761 1341 1821
Fragments arranged by size:
1821 1341 761
BclI T'GATC_A
Cuts at: 1443 1443
Size: 3923
BglI GCCn_nnn'nGGC
Cuts at: 347 761 347
Size: 414 3509
BglII A'GATC_T
Cuts at: 1438 3475 1438
Size: 2037 1886
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 210 252 1548 1641 2697 210
Size: 42 1296 93 1056 1436
Fragments arranged by size:
1436 1296 1056 93 42
BmrI ACTGGGnnnn_n'
Cuts at: 463 760 1135 1216 1551 3695 463
Size: 297 375 81 335 2144 691
Fragments arranged by size:
2144 691 375 335 297 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 429 2290 429
Size: 1861 2062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 290 460 510 274
Size: 16 170 50 3687
Fragments arranged by size:
3687 170 50 16
Bpu10I CC'TnA_GC
Cuts at: 773 773
Size: 3923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1978 2470 2768 1099
Size: 879 492 298 2254
Fragments arranged by size:
2254 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 325 403 3627 325
Size: 78 3224 621
Fragments arranged by size:
3224 621 78
BsaAI yAC'GTr
Cuts at: 1276 1276
Size: 3923
BsaBI GATnn'nnATC
Cuts at: 6 1437 3474 6
Size: 1431 2037 455
Fragments arranged by size:
2037 1431 455
BsaHI Gr'CG_yC
Cuts at: 899 1354 1602 2304 3800 899
Size: 455 248 702 1496 1022
Fragments arranged by size:
1496 1022 702 455 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2585 2732 3143 1112
Size: 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 637 667 637
Size: 30 3893
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 421 700 421
Size: 279 3644
BseYI C'CCAG_C
Cuts at: 175 743 858 1348 2106 2683 3179 175
Size: 568 115 490 758 577 496 919
Fragments arranged by size:
919 758 577 568 496 490 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 872 804
Size: 68 3855
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2719 104
Size: 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 266 889 937 1715 1905 2697 266
Size: 623 48 778 190 792 1492
Fragments arranged by size:
1492 792 778 623 190 48
Bsp1286I G_dGCh'C
Cuts at: 210 252 266 889 937 1548 1641 1715 1905
Size: 42 14 623 48 611 93 74 190
Cuts at: 1905 1967 2697 210
Size: 62 730 1436
Fragments arranged by size:
1436 730 623 611 190 93 74 62
48 42 14
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 3099 3099
Size: 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 500 973 1187 1489 1870 500
Size: 473 214 302 381 2553
Fragments arranged by size:
2553 473 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2215 2269 3753 3918 50
Size: 2165 54 1484 165 55
Fragments arranged by size:
2165 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1835 1835
Size: 3923
BsrFI r'CCGG_y
Cuts at: 1921 2102 2311 1921
Size: 181 209 3533
Fragments arranged by size:
3533 209 181
BssHII G'CGCG_C
Cuts at: 186 186
Size: 3923
BssSI C'ACGA_G
Cuts at: 838 2194 2552 838
Size: 1356 358 2209
Fragments arranged by size:
2209 1356 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 1300
Size: 3923
BstBI TT'CG_AA
Cuts at: 2284 2284
Size: 3923
BstXI CCAn_nnnn'nTGG
Cuts at: 534 609 534
Size: 75 3848
BstYI r'GATC_y
Cuts at: 1 948 1415 1438 1773 2019 3031 3117 3129
Size: 947 467 23 335 246 1012 86 12
Cuts at: 3129 3475 1
Size: 346 449
Fragments arranged by size:
1012 947 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 404 404
Size: 3923
BtgI C'CryG_G
Cuts at: 107 893 107
Size: 786 3137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1873 2054 2217 1873
Size: 181 163 3579
Fragments arranged by size:
3579 181 163
BtsI GCAGTG_nn'
Cuts at: 797 1166 797
Size: 369 3554
DraI TTT'AAA
Cuts at: 922 922
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 1629 2487 1629
Size: 858 3065
EaeI y'GGCC_r
Cuts at: 348 378 546 572 1508 1682 2073 2100 3445
Size: 30 168 26 936 174 391 27 1345
Cuts at: 3445 348
Size: 826
Fragments arranged by size:
1345 936 826 391 174 168 30 27
26
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 493 1946 2156 493
Size: 1453 210 2260
Fragments arranged by size:
2260 1453 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 328 418 2131 2441 2587 3733 328
Size: 90 1713 310 146 1146 518
Fragments arranged by size:
1713 1146 518 310 146 90
EcoICRI GAG'CTC
Cuts at: 264 935 264
Size: 671 3252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 290 460 510 1748 2180 2927 3195 274
Size: 16 170 50 1238 432 747 268 1002
Fragments arranged by size:
1238 1002 747 432 268 170 50 16
EcoNI CCTnn'n_nnAGG
Cuts at: 256 256
Size: 3923
EcoO109I rG'GnC_Cy
Cuts at: 206 249 1062 206
Size: 43 813 3067
Fragments arranged by size:
3067 813 43
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
EcoRV GAT'ATC
Cuts at: 909 909
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3923
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 1357 1605 2627 3606 1357
Size: 248 1022 979 1674
Fragments arranged by size:
1674 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 164 196 203 235 1765 1795 1797 1827 164
Size: 32 7 32 1530 30 2 30 2260
Fragments arranged by size:
2260 1530 32 32 30 30 7 2
HincII GTy'rAC
Cuts at: 369 645 962 369
Size: 276 317 3330
Fragments arranged by size:
3330 317 276
HindIII A'AGCT_T
Cuts at: 605 605
Size: 3923
KasI G'GCGC_C
Cuts at: 1353 1601 1353
Size: 248 3675
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 862 2594 2778 862
Size: 1732 184 2007
Fragments arranged by size:
2007 1732 184
MscI TGG'CCA
Cuts at: 380 574 1684 380
Size: 194 1110 2619
Fragments arranged by size:
2619 1110 194
MslI CAynn'nnrTG
Cuts at: 892 1912 2039 892
Size: 1020 127 2776
Fragments arranged by size:
2776 1020 127
MspA1I CmG'CkG
Cuts at: 203 435 678 772 1034 1348 1708 2721 2966
Size: 232 243 94 262 314 360 1013 245
Cuts at: 2966 203
Size: 1160
Fragments arranged by size:
1160 1013 360 314 262 245 243 232
94
NaeI GCC'GGC
Cuts at: 2104 2104
Size: 3923
NarI GG'CG_CC
Cuts at: 1354 1602 1354
Size: 248 3675
NcoI C'CATG_G
Cuts at: 107 107
Size: 3923
NdeI CA'TA_TG
Cuts at: 630 630
Size: 3923
NgoMIV G'CCGG_C
Cuts at: 2102 2102
Size: 3923
NspI r_CATG'y
Cuts at: 738 976 1476 2383 738
Size: 238 500 907 2278
Fragments arranged by size:
2278 907 500 238
PasI CC'CwG_GG
Cuts at: 420 420
Size: 3923
PciI A'CATG_T
Cuts at: 2379 2379
Size: 3923
PflMI CCAn_nnn'nTGG
Cuts at: 438 750 438
Size: 312 3611
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3891
PspOMI G'GGCC_C
Cuts at: 206 248 206
Size: 42 3881
PstI C_TGCA'G
Cuts at: 970 1655 970
Size: 685 3238
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 435 1348 1708 435
Size: 913 360 2650
Fragments arranged by size:
2650 913 360
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 266 937 266
Size: 671 3252
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SfcI C'TryA_G
Cuts at: 33 966 1651 2644 2835 33
Size: 933 685 993 191 1121
Fragments arranged by size:
1121 993 933 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 761 761
Size: 3923
SfoI GGC'GCC
Cuts at: 1355 1603 1355
Size: 248 3675
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 945 945
Size: 3923
SmlI C'TyrA_G
Cuts at: 1078 1993 2485 2747 1078
Size: 915 492 262 2254
Fragments arranged by size:
2254 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 738 976 1476 738
Size: 238 500 3185
Fragments arranged by size:
3185 500 238
StuI AGG'CCT
Cuts at: 402 402
Size: 3923
StyI C'CwwG_G
Cuts at: 107 578 712 1057 1411 107
Size: 471 134 345 354 2619
Fragments arranged by size:
2619 471 354 345 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 401 1506 1639 2098 2307 401
Size: 1105 133 459 209 2017
Fragments arranged by size:
2017 1105 459 209 133
TatI w'GTAC_w
Cuts at: 3438 3438
Size: 3923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 3891
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 665 1418 665
Size: 753 3170
XmaI C'CCGG_G
Cuts at: 943 943
Size: 3923
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I ClaI CspCI DraIII FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI