Restriction map for
pF1KB6377
(Circular) (Six-base) MAPSORT of: pF1KB6377.seq Check: 6993 from: 1 to: 3926
pF1KB6377 3926 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 964 964
Size: 3926
Acc65I G'GTAC_C
Cuts at: 942 942
Size: 3926
AclI AA'CG_TT
Cuts at: 3766 3766
Size: 3926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 370 739 1751 2183 2930 3198 160
Size: 210 369 1012 432 747 268 888
Fragments arranged by size:
1012 888 747 432 369 268 210
AflIII A'CryG_T
Cuts at: 125 1278 2382 3359 125
Size: 1153 1104 977 692
Fragments arranged by size:
1153 1104 977 692
AgeI A'CCGG_T
Cuts at: 2314 2314
Size: 3926
AlwNI CAG_nnn'CTG
Cuts at: 906 1231 2798 906
Size: 325 1567 2034
Fragments arranged by size:
2034 1567 325
ApaLI G'TGCA_C
Cuts at: 2696 2696
Size: 3926
ApoI r'AATT_y
Cuts at: 505 601 930 3279 3535 505
Size: 96 329 2349 256 896
Fragments arranged by size:
2349 896 329 256 96
AseI AT'TA_AT
Cuts at: 20 20
Size: 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
AvaI C'yCGr_G
Cuts at: 946 946
Size: 3926
AvrII C'CTAG_G
Cuts at: 439 439
Size: 3926
BamHI G'GATC_C
Cuts at: 951 951
Size: 3926
BanI G'GyrC_C
Cuts at: 942 1356 1604 1639 942
Size: 414 248 35 3229
Fragments arranged by size:
3229 414 248 35
BanII G_rGCy'C
Cuts at: 940 1970 940
Size: 1030 2896
BbeI G_GCGC'C
Cuts at: 1360 1608 1360
Size: 248 3678
BbsI GAAGACnn'nnnn_
Cuts at: 140 173 707 140
Size: 33 534 3359
Fragments arranged by size:
3359 534 33
BciVI GTATCCnnnnn_n'
Cuts at: 1824 2585 3926 1824
Size: 761 1341 1824
Fragments arranged by size:
1824 1341 761
BclI T'GATC_A
Cuts at: 183 552 1446 183
Size: 369 894 2663
Fragments arranged by size:
2663 894 369
BglII A'GATC_T
Cuts at: 1441 3478 1441
Size: 2037 1889
BlpI GC'TnA_GC
Cuts at: 1038 1038
Size: 3926
Bme1580I G_kGCm'C
Cuts at: 1551 1644 2700 1551
Size: 93 1056 2777
Fragments arranged by size:
2777 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 867 1138 1219 1554 3698 867
Size: 271 81 335 2144 1095
Fragments arranged by size:
2144 1095 335 271 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 3926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 418
Size: 3926
Bpu10I CC'TnA_GC
Cuts at: 463 463
Size: 3926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1981 2473 2771 1102
Size: 879 492 298 2257
Fragments arranged by size:
2257 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 754 3630 754
Size: 2876 1050
BsaAI yAC'GTr
Cuts at: 1279 1279
Size: 3926
BsaBI GATnn'nnATC
Cuts at: 6 1440 3477 6
Size: 1434 2037 455
Fragments arranged by size:
2037 1434 455
BsaHI Gr'CG_yC
Cuts at: 521 1357 1605 2307 3803 521
Size: 836 248 702 1496 644
Fragments arranged by size:
1496 836 702 644 248
BsaWI w'CCGG_w
Cuts at: 1115 1636 2314 2588 2735 3146 1115
Size: 521 678 274 147 411 1895
Fragments arranged by size:
1895 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 160 190 160
Size: 30 3896
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 760 760
Size: 3926
BseYI C'CCAG_C
Cuts at: 1351 2109 2686 3182 1351
Size: 758 577 496 2095
Fragments arranged by size:
2095 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1468 1514 2722 104
Size: 1364 46 1208 1308
Fragments arranged by size:
1364 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 940 1718 1908 2700 940
Size: 778 190 792 2166
Fragments arranged by size:
2166 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 351 351
Size: 3926
Bsp1286I G_dGCh'C
Cuts at: 940 1551 1644 1718 1908 1970 2700 940
Size: 611 93 74 190 62 730 2166
Fragments arranged by size:
2166 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3146 3146
Size: 3926
BspHI T'CATG_A
Cuts at: 3102 3102
Size: 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 976 1190 1492 1873 976
Size: 214 302 381 3029
Fragments arranged by size:
3029 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2218 2272 3756 3921 50
Size: 2168 54 1484 165 55
Fragments arranged by size:
2168 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 474 1838 474
Size: 1364 2562
BsrFI r'CCGG_y
Cuts at: 1924 2105 2314 1924
Size: 181 209 3536
Fragments arranged by size:
3536 209 181
BssSI C'ACGA_G
Cuts at: 2197 2555 2197
Size: 358 3568
BstAPI GCAn_nnn'nTGC
Cuts at: 1303 1303
Size: 3926
BstBI TT'CG_AA
Cuts at: 2287 2287
Size: 3926
BstYI r'GATC_y
Cuts at: 1 951 1418 1441 1776 2022 3034 3120 3132
Size: 950 467 23 335 246 1012 86 12
Cuts at: 3132 3478 1
Size: 346 449
Fragments arranged by size:
1012 950 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3926
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 390 1876 2057 2220 390
Size: 1486 181 163 2096
Fragments arranged by size:
2096 1486 181 163
BtsI GCAGTG_nn'
Cuts at: 602 1169 602
Size: 567 3359
DraI TTT'AAA
Cuts at: 925 925
Size: 3926
DrdI GACnn_nn'nnGTC
Cuts at: 1632 2490 1632
Size: 858 3068
EaeI y'GGCC_r
Cuts at: 110 1511 1685 2076 2103 3448 110
Size: 1401 174 391 27 1345 588
Fragments arranged by size:
1401 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1511 1511
Size: 3926
EarI CTCTTCn'nnn_
Cuts at: 349 778 1949 2159 349
Size: 429 1171 210 2116
Fragments arranged by size:
2116 1171 429 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2134 2444 2590 3736 2134
Size: 310 146 1146 2324
Fragments arranged by size:
2324 1146 310 146
EcoICRI GAG'CTC
Cuts at: 938 938
Size: 3926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 370 418 739 1751 2183 2930 3198 160
Size: 210 48 321 1012 432 747 268 888
Fragments arranged by size:
1012 888 747 432 321 268 210 48
EcoO109I rG'GnC_Cy
Cuts at: 1065 1065
Size: 3926
EcoRI G'AATT_C
Cuts at: 505 930 505
Size: 425 3501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 321 1945 321
Size: 1624 2302
FspI TGC'GCA
Cuts at: 1707 1707
Size: 3926
HaeII r_GCGC'y
Cuts at: 1360 1608 2630 3609 1360
Size: 248 1022 979 1677
Fragments arranged by size:
1677 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 175 192 207 1768 1798 1800 1830 160
Size: 15 17 15 1561 30 2 30 2256
Fragments arranged by size:
2256 1561 30 30 17 15 15 2
HincII GTy'rAC
Cuts at: 965 965
Size: 3926
HindIII A'AGCT_T
Cuts at: 298 298
Size: 3926
KasI G'GCGC_C
Cuts at: 1356 1604 1356
Size: 248 3678
KpnI G_GTAC'C
Cuts at: 946 946
Size: 3926
MfeI C'AATT_G
Cuts at: 676 676
Size: 3926
MluI A'CGCG_T
Cuts at: 3359 3359
Size: 3926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2597 2781 2597
Size: 184 3742
MscI TGG'CCA
Cuts at: 112 1687 112
Size: 1575 2351
MslI CAynn'nnrTG
Cuts at: 1915 2042 1915
Size: 127 3799
MspA1I CmG'CkG
Cuts at: 1037 1351 1711 2724 2969 1037
Size: 314 360 1013 245 1994
Fragments arranged by size:
1994 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 3926
NarI GG'CG_CC
Cuts at: 1357 1605 1357
Size: 248 3678
NcoI C'CATG_G
Cuts at: 107 107
Size: 3926
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 3926
NspI r_CATG'y
Cuts at: 129 979 1479 2386 129
Size: 850 500 907 1669
Fragments arranged by size:
1669 907 850 500
PciI A'CATG_T
Cuts at: 125 2382 125
Size: 2257 1669
PmeI GTTT'AAAC
Cuts at: 925 925
Size: 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3894
PsiI TTA'TAA
Cuts at: 620 620
Size: 3926
PstI C_TGCA'G
Cuts at: 973 1658 973
Size: 685 3241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3926
PvuII CAG'CTG
Cuts at: 1351 1711 1351
Size: 360 3566
RsrII CG'GwC_CG
Cuts at: 2121 2121
Size: 3926
SacI G_AGCT'C
Cuts at: 940 940
Size: 3926
SalI G'TCGA_C
Cuts at: 963 963
Size: 3926
SapI GCTCTTCn'nnn_
Cuts at: 1949 2159 1949
Size: 210 3716
SbfI CC_TGCA'GG
Cuts at: 973 973
Size: 3926
ScaI AGT'ACT
Cuts at: 3443 3443
Size: 3926
SfcI C'TryA_G
Cuts at: 33 969 1654 2647 2838 33
Size: 936 685 993 191 1121
Fragments arranged by size:
1121 993 936 685 191
SfoI GGC'GCC
Cuts at: 1358 1606 1358
Size: 248 3678
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
SmaI CCC'GGG
Cuts at: 948 948
Size: 3926
SmlI C'TyrA_G
Cuts at: 1081 1996 2488 2750 1081
Size: 915 492 262 2257
Fragments arranged by size:
2257 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3926
SphI G_CATG'C
Cuts at: 979 1479 979
Size: 500 3426
StyI C'CwwG_G
Cuts at: 107 439 1060 1414 107
Size: 332 621 354 2619
Fragments arranged by size:
2619 621 354 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 601 849 1509 1642 2101 2310 601
Size: 248 660 133 459 209 2217
Fragments arranged by size:
2217 660 459 248 209 133
TatI w'GTAC_w
Cuts at: 3441 3441
Size: 3926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 3894
Tth111I GACn'n_nGTC
Cuts at: 1723 1723
Size: 3926
XbaI T'CTAG_A
Cuts at: 957 957
Size: 3926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1421 1421
Size: 3926
XmaI C'CCGG_G
Cuts at: 946 946
Size: 3926
XmnI GAAnn'nnTTC
Cuts at: 208 805 208
Size: 597 3329
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI