Restriction map for
pF1KB6379
(Circular) (Six-base) MAPSORT of: pF1KB6379.seq Check: 3313 from: 1 to: 3797
pF1KB6379 3797 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 418 418
Size: 3797
AatII G_ACGT'C
Cuts at: 128 128
Size: 3797
AccI GT'mk_AC
Cuts at: 835 835
Size: 3797
Acc65I G'GTAC_C
Cuts at: 813 813
Size: 3797
AclI AA'CG_TT
Cuts at: 3637 3637
Size: 3797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1622 2054 2801 3069 436
Size: 1186 432 747 268 1164
Fragments arranged by size:
1186 1164 747 432 268
AflII C'TTAA_G
Cuts at: 216 468 216
Size: 252 3545
AflIII A'CryG_T
Cuts at: 1149 2253 3230 1149
Size: 1104 977 1716
Fragments arranged by size:
1716 1104 977
AgeI A'CCGG_T
Cuts at: 2185 2185
Size: 3797
AlwNI CAG_nnn'CTG
Cuts at: 288 1102 2669 288
Size: 814 1567 1416
Fragments arranged by size:
1567 1416 814
ApaLI G'TGCA_C
Cuts at: 2567 2567
Size: 3797
ApoI r'AATT_y
Cuts at: 160 256 634 801 3150 3406 160
Size: 96 378 167 2349 256 551
Fragments arranged by size:
2349 551 378 256 167 96
AseI AT'TA_AT
Cuts at: 20 20
Size: 3797
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
AvaI C'yCGr_G
Cuts at: 817 817
Size: 3797
BamHI G'GATC_C
Cuts at: 822 822
Size: 3797
BanI G'GyrC_C
Cuts at: 652 813 1227 1475 1510 652
Size: 161 414 248 35 2939
Fragments arranged by size:
2939 414 248 161 35
BanII G_rGCy'C
Cuts at: 811 1841 811
Size: 1030 2767
BbeI G_GCGC'C
Cuts at: 1231 1479 1231
Size: 248 3549
BciVI GTATCCnnnnn_n'
Cuts at: 1695 2456 3797 1695
Size: 761 1341 1695
Fragments arranged by size:
1695 1341 761
BclI T'GATC_A
Cuts at: 1317 1317
Size: 3797
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3797
BglII A'GATC_T
Cuts at: 462 1312 3349 462
Size: 850 2037 910
Fragments arranged by size:
2037 910 850
BlpI GC'TnA_GC
Cuts at: 373 909 373
Size: 536 3261
Bme1580I G_kGCm'C
Cuts at: 655 1422 1515 2571 655
Size: 767 93 1056 1881
Fragments arranged by size:
1881 1056 767 93
BmgBI CAC'GTC
Cuts at: 132 132
Size: 3797
BmrI ACTGGGnnnn_n'
Cuts at: 1009 1090 1425 3569 1009
Size: 81 335 2144 1237
Fragments arranged by size:
2144 1237 335 81
BmtI G_CTAG'C
Cuts at: 736 736
Size: 3797
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 481 2164 481
Size: 1683 2114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 310 325 190
Size: 120 15 3662
Fragments arranged by size:
3662 120 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1852 2344 2642 973
Size: 879 492 298 2128
Fragments arranged by size:
2128 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3501 3501
Size: 3797
BsaAI yAC'GTr
Cuts at: 1150 1150
Size: 3797
BsaBI GATnn'nnATC
Cuts at: 6 1311 3348 6
Size: 1305 2037 455
Fragments arranged by size:
2037 1305 455
BsaHI Gr'CG_yC
Cuts at: 125 1228 1476 2178 3674 125
Size: 1103 248 702 1496 248
Fragments arranged by size:
1496 1103 702 248 248
BsaWI w'CCGG_w
Cuts at: 134 986 1507 2185 2459 2606 3017 134
Size: 852 521 678 274 147 411 914
Fragments arranged by size:
914 852 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 323 353 323
Size: 30 3767
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 473 592 473
Size: 119 3678
BseYI C'CCAG_C
Cuts at: 557 663 1222 1980 2557 3053 557
Size: 106 559 758 577 496 1301
Fragments arranged by size:
1301 758 577 559 496 106
BsiEI CG_ry'CG
Cuts at: 104 1339 1385 2593 104
Size: 1235 46 1208 1308
Fragments arranged by size:
1308 1235 1208 46
BsiHKAI G_wGCw'C
Cuts at: 225 811 1589 1779 2571 225
Size: 586 778 190 792 1451
Fragments arranged by size:
1451 792 778 586 190
Bsp1286I G_dGCh'C
Cuts at: 225 655 811 1422 1515 1589 1779 1841 2571
Size: 430 156 611 93 74 190 62 730
Cuts at: 2571 225
Size: 1451
Fragments arranged by size:
1451 730 611 430 190 156 93 74
62
BspEI T'CCGG_A
Cuts at: 134 3017 134
Size: 2883 914
BspHI T'CATG_A
Cuts at: 2973 2973
Size: 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 418 552 847 1061 1363 1744 418
Size: 134 295 214 302 381 2471
Fragments arranged by size:
2471 381 302 295 214 134
BsrBI CCG'CTC
Cuts at: 50 2089 2143 3627 3792 50
Size: 2039 54 1484 165 55
Fragments arranged by size:
2039 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1709 1709
Size: 3797
BsrFI r'CCGG_y
Cuts at: 1795 1976 2185 1795
Size: 181 209 3407
Fragments arranged by size:
3407 209 181
BssSI C'ACGA_G
Cuts at: 2068 2426 2068
Size: 358 3439
BstAPI GCAn_nnn'nTGC
Cuts at: 1174 1174
Size: 3797
BstBI TT'CG_AA
Cuts at: 2158 2158
Size: 3797
BstXI CCAn_nnnn'nTGG
Cuts at: 663 663
Size: 3797
BstYI r'GATC_y
Cuts at: 1 137 462 822 1289 1312 1647 1893 2905
Size: 136 325 360 467 23 335 246 1012
Cuts at: 2905 2991 3003 3349 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 360 346 335 325 246
136 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3797
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1747 1928 2091 1747
Size: 181 163 3453
Fragments arranged by size:
3453 181 163
BtsI GCAGTG_nn'
Cuts at: 1040 1040
Size: 3797
DraI TTT'AAA
Cuts at: 796 796
Size: 3797
DrdI GACnn_nn'nnGTC
Cuts at: 130 1503 2361 130
Size: 1373 858 1566
Fragments arranged by size:
1566 1373 858
EaeI y'GGCC_r
Cuts at: 1382 1556 1947 1974 3319 1382
Size: 174 391 27 1345 1860
Fragments arranged by size:
1860 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1382 1382
Size: 3797
EarI CTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2005 2315 2461 3607 2005
Size: 310 146 1146 2195
Fragments arranged by size:
2195 1146 310 146
EcoICRI GAG'CTC
Cuts at: 809 809
Size: 3797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 310 325 436 1622 2054 2801 3069 190
Size: 120 15 111 1186 432 747 268 918
Fragments arranged by size:
1186 918 747 432 268 120 111 15
EcoNI CCTnn'n_nnAGG
Cuts at: 390 390
Size: 3797
EcoO109I rG'GnC_Cy
Cuts at: 211 285 936 211
Size: 74 651 3072
Fragments arranged by size:
3072 651 74
EcoRI G'AATT_C
Cuts at: 801 801
Size: 3797
EcoRV GAT'ATC
Cuts at: 195 195
Size: 3797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 463 795 1816 463
Size: 332 1021 2444
Fragments arranged by size:
2444 1021 332
FspI TGC'GCA
Cuts at: 1578 1578
Size: 3797
HaeII r_GCGC'y
Cuts at: 184 1231 1479 2501 3480 184
Size: 1047 248 1022 979 501
Fragments arranged by size:
1047 1022 979 501 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 304 336 1639 1669 1671 1701 304
Size: 32 1303 30 2 30 2400
Fragments arranged by size:
2400 1303 32 30 30 2
HincII GTy'rAC
Cuts at: 836 836
Size: 3797
HindIII A'AGCT_T
Cuts at: 673 712 673
Size: 39 3758
KasI G'GCGC_C
Cuts at: 1227 1475 1227
Size: 248 3549
KpnI G_GTAC'C
Cuts at: 817 817
Size: 3797
MluI A'CGCG_T
Cuts at: 3230 3230
Size: 3797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2652 2468
Size: 184 3613
MscI TGG'CCA
Cuts at: 1558 1558
Size: 3797
MslI CAynn'nnrTG
Cuts at: 1786 1913 1786
Size: 127 3670
MspA1I CmG'CkG
Cuts at: 663 908 1222 1582 2595 2840 663
Size: 245 314 360 1013 245 1620
Fragments arranged by size:
1620 1013 360 314 245 245
NaeI GCC'GGC
Cuts at: 1978 1978
Size: 3797
NarI GG'CG_CC
Cuts at: 1228 1476 1228
Size: 248 3549
NcoI C'CATG_G
Cuts at: 107 107
Size: 3797
NgoMIV G'CCGG_C
Cuts at: 1976 1976
Size: 3797
NheI G'CTAG_C
Cuts at: 732 732
Size: 3797
NspI r_CATG'y
Cuts at: 850 1350 2257 850
Size: 500 907 2390
Fragments arranged by size:
2390 907 500
PciI A'CATG_T
Cuts at: 2253 2253
Size: 3797
PflMI CCAn_nnn'nTGG
Cuts at: 348 348
Size: 3797
PfoI T'CCnGG_A
Cuts at: 341 617 341
Size: 276 3521
PmeI GTTT'AAAC
Cuts at: 796 796
Size: 3797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 3765
PpuMI rG'GwC_Cy
Cuts at: 211 285 211
Size: 74 3723
PstI C_TGCA'G
Cuts at: 844 1529 844
Size: 685 3112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3797
PvuII CAG'CTG
Cuts at: 663 1222 1582 663
Size: 559 360 2878
Fragments arranged by size:
2878 559 360
RsrII CG'GwC_CG
Cuts at: 1992 1992
Size: 3797
SacI G_AGCT'C
Cuts at: 811 811
Size: 3797
SalI G'TCGA_C
Cuts at: 834 834
Size: 3797
SapI GCTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
SbfI CC_TGCA'GG
Cuts at: 844 844
Size: 3797
ScaI AGT'ACT
Cuts at: 3314 3314
Size: 3797
SfcI C'TryA_G
Cuts at: 33 840 1525 2518 2709 33
Size: 807 685 993 191 1121
Fragments arranged by size:
1121 993 807 685 191
SfoI GGC'GCC
Cuts at: 1229 1477 1229
Size: 248 3549
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
SmaI CCC'GGG
Cuts at: 819 819
Size: 3797
SmlI C'TyrA_G
Cuts at: 216 468 952 1867 2359 2621 216
Size: 252 484 915 492 262 1392
Fragments arranged by size:
1392 915 492 484 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3797
SphI G_CATG'C
Cuts at: 850 1350 850
Size: 500 3297
StuI AGG'CCT
Cuts at: 385 385
Size: 3797
StyI C'CwwG_G
Cuts at: 107 931 1285 107
Size: 824 354 2619
Fragments arranged by size:
2619 824 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1380 1513 1972 2181 1380
Size: 133 459 209 2996
Fragments arranged by size:
2996 459 209 133
TatI w'GTAC_w
Cuts at: 3312 3312
Size: 3797
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 599 631 3314 3346 599
Size: 32 2683 32 1050
Fragments arranged by size:
2683 1050 32 32
Tth111I GACn'n_nGTC
Cuts at: 1594 1594
Size: 3797
XbaI T'CTAG_A
Cuts at: 432 828 432
Size: 396 3401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 495 1292 495
Size: 797 3000
XmaI C'CCGG_G
Cuts at: 817 817
Size: 3797
XmnI GAAnn'nnTTC
Cuts at: 449 449
Size: 3797
ZraI GAC'GTC
Cuts at: 126 126
Size: 3797
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI Bpu10I BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI