Restriction map for
pF1KB6381
(Circular) (Six-base) MAPSORT of: pF1KB6381.seq Check: 8262 from: 1 to: 3929
pF1KB6381 3929 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 967 967
Size: 3929
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 511 3769 511
Size: 3258 671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1754 2186 2933 3201 1754
Size: 432 747 268 2482
Fragments arranged by size:
2482 747 432 268
AflIII A'CryG_T
Cuts at: 1281 2385 3362 1281
Size: 1104 977 1848
Fragments arranged by size:
1848 1104 977
AgeI A'CCGG_T
Cuts at: 2317 2317
Size: 3929
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 678 710 678
Size: 32 3897
AlwNI CAG_nnn'CTG
Cuts at: 865 1234 2801 865
Size: 369 1567 1993
Fragments arranged by size:
1993 1567 369
ApaLI G'TGCA_C
Cuts at: 2699 2699
Size: 3929
ApoI r'AATT_y
Cuts at: 235 447 933 3282 3538 235
Size: 212 486 2349 256 626
Fragments arranged by size:
2349 626 486 256 212
AseI AT'TA_AT
Cuts at: 20 634 20
Size: 614 3315
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 949 949
Size: 3929
BamHI G'GATC_C
Cuts at: 243 954 243
Size: 711 3218
BanI G'GyrC_C
Cuts at: 945 1359 1607 1642 945
Size: 414 248 35 3232
Fragments arranged by size:
3232 414 248 35
BanII G_rGCy'C
Cuts at: 330 943 1973 330
Size: 613 1030 2286
Fragments arranged by size:
2286 1030 613
BbeI G_GCGC'C
Cuts at: 1363 1611 1363
Size: 248 3681
BciVI GTATCCnnnnn_n'
Cuts at: 1827 2588 3929 1827
Size: 761 1341 1827
Fragments arranged by size:
1827 1341 761
BclI T'GATC_A
Cuts at: 1449 1449
Size: 3929
BglII A'GATC_T
Cuts at: 847 1444 3481 847
Size: 597 2037 1295
Fragments arranged by size:
2037 1295 597
BlpI GC'TnA_GC
Cuts at: 1041 1041
Size: 3929
Bme1580I G_kGCm'C
Cuts at: 1554 1647 2703 1554
Size: 93 1056 2780
Fragments arranged by size:
2780 1056 93
BmgBI CAC'GTC
Cuts at: 165 165
Size: 3929
BmrI ACTGGGnnnn_n'
Cuts at: 1141 1222 1557 3701 1141
Size: 81 335 2144 1369
Fragments arranged by size:
2144 1369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 579 2296 579
Size: 1717 2212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 842 842
Size: 3929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 1105 1984 2476 2774 441
Size: 664 879 492 298 1596
Fragments arranged by size:
1596 879 664 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 680 710 809 3633 680
Size: 30 99 2824 976
Fragments arranged by size:
2824 976 99 30
BsaAI yAC'GTr
Cuts at: 1282 1282
Size: 3929
BsaBI GATnn'nnATC
Cuts at: 6 206 1443 3480 6
Size: 200 1237 2037 455
Fragments arranged by size:
2037 1237 455 200
BsaHI Gr'CG_yC
Cuts at: 1360 1608 2310 3806 1360
Size: 248 702 1496 1483
Fragments arranged by size:
1496 1483 702 248
BsaWI w'CCGG_w
Cuts at: 1118 1639 2317 2591 2738 3149 1118
Size: 521 678 274 147 411 1898
Fragments arranged by size:
1898 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 499 1354 2112 2689 3185 499
Size: 855 758 577 496 1243
Fragments arranged by size:
1243 855 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1471 1517 2725 104
Size: 1367 46 1208 1308
Fragments arranged by size:
1367 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 943 1721 1911 2703 943
Size: 778 190 792 2169
Fragments arranged by size:
2169 792 778 190
BsmI GAATG_Cn'
Cuts at: 588 588
Size: 3929
Bsp1286I G_dGCh'C
Cuts at: 330 943 1554 1647 1721 1911 1973 2703 330
Size: 613 611 93 74 190 62 730 1556
Fragments arranged by size:
1556 730 613 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3149 3149
Size: 3929
BspHI T'CATG_A
Cuts at: 488 3105 488
Size: 2617 1312
BspMI ACCTGCnnnn'nnnn_
Cuts at: 662 979 1193 1495 1876 662
Size: 317 214 302 381 2715
Fragments arranged by size:
2715 381 317 302 214
BsrBI CCG'CTC
Cuts at: 50 2221 2275 3759 3924 50
Size: 2171 54 1484 165 55
Fragments arranged by size:
2171 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 927 1841 927
Size: 914 3015
BsrFI r'CCGG_y
Cuts at: 746 1927 2108 2317 746
Size: 1181 181 209 2358
Fragments arranged by size:
2358 1181 209 181
BssSI C'ACGA_G
Cuts at: 335 2200 2558 335
Size: 1865 358 1706
Fragments arranged by size:
1865 1706 358
BstAPI GCAn_nnn'nTGC
Cuts at: 262 1306 262
Size: 1044 2885
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstXI CCAn_nnnn'nTGG
Cuts at: 832 832
Size: 3929
BstYI r'GATC_y
Cuts at: 1 243 474 847 954 1421 1444 1779 2025
Size: 242 231 373 107 467 23 335 246
Cuts at: 2025 3037 3123 3135 3481 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 373 346 335 246 242
231 107 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 2060 2223 1879
Size: 181 163 3585
Fragments arranged by size:
3585 181 163
BtsI GCAGTG_nn'
Cuts at: 1172 1172
Size: 3929
DraI TTT'AAA
Cuts at: 928 928
Size: 3929
DraIII CAC_nnn'GTG
Cuts at: 340 340
Size: 3929
DrdI GACnn_nn'nnGTC
Cuts at: 1635 2493 1635
Size: 858 3071
EaeI y'GGCC_r
Cuts at: 149 1514 1688 2079 2106 3451 149
Size: 1365 174 391 27 1345 627
Fragments arranged by size:
1365 1345 627 391 174 27
EagI C'GGCC_G
Cuts at: 1514 1514
Size: 3929
EarI CTCTTCn'nnn_
Cuts at: 339 814 1952 2162 339
Size: 475 1138 210 2106
Fragments arranged by size:
2106 1138 475 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2137 2447 2593 3739 2137
Size: 310 146 1146 2327
Fragments arranged by size:
2327 1146 310 146
EcoICRI GAG'CTC
Cuts at: 941 941
Size: 3929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 842 1754 2186 2933 3201 842
Size: 912 432 747 268 1570
Fragments arranged by size:
1570 912 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 881 1068 881
Size: 187 3742
EcoRI G'AATT_C
Cuts at: 933 933
Size: 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3929
FspI TGC'GCA
Cuts at: 1710 1710
Size: 3929
HaeII r_GCGC'y
Cuts at: 1363 1611 2633 3612 1363
Size: 248 1022 979 1680
Fragments arranged by size:
1680 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 126 134 158 436 468 1771 1801 1803
Size: 24 8 24 278 32 1303 30 2
Cuts at: 1803 1833 102
Size: 30 2198
Fragments arranged by size:
2198 1303 278 32 30 30 24 24
8 2
HincII GTy'rAC
Cuts at: 912 968 912
Size: 56 3873
KasI G'GCGC_C
Cuts at: 1359 1607 1359
Size: 248 3681
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MluI A'CGCG_T
Cuts at: 3362 3362
Size: 3929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2600 2784 2600
Size: 184 3745
MscI TGG'CCA
Cuts at: 1690 1690
Size: 3929
MslI CAynn'nnrTG
Cuts at: 1918 2045 1918
Size: 127 3802
MspA1I CmG'CkG
Cuts at: 148 467 1040 1354 1714 2727 2972 148
Size: 319 573 314 360 1013 245 1105
Fragments arranged by size:
1105 1013 573 360 319 314 245
NaeI GCC'GGC
Cuts at: 2110 2110
Size: 3929
NarI GG'CG_CC
Cuts at: 1360 1608 1360
Size: 248 3681
NgoMIV G'CCGG_C
Cuts at: 2108 2108
Size: 3929
NspI r_CATG'y
Cuts at: 322 982 1482 2389 322
Size: 660 500 907 1862
Fragments arranged by size:
1862 907 660 500
PciI A'CATG_T
Cuts at: 2385 2385
Size: 3929
PfoI T'CCnGG_A
Cuts at: 682 682
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3897
PpuMI rG'GwC_Cy
Cuts at: 881 881
Size: 3929
PstI C_TGCA'G
Cuts at: 976 1661 976
Size: 685 3244
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 467 1354 1714 467
Size: 887 360 2682
Fragments arranged by size:
2682 887 360
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 943 943
Size: 3929
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 814 1952 2162 814
Size: 1138 210 2581
Fragments arranged by size:
2581 1138 210
SbfI CC_TGCA'GG
Cuts at: 976 976
Size: 3929
ScaI AGT'ACT
Cuts at: 3446 3446
Size: 3929
SfcI C'TryA_G
Cuts at: 33 294 972 1657 2650 2841 33
Size: 261 678 685 993 191 1121
Fragments arranged by size:
1121 993 685 678 261 191
SfoI GGC'GCC
Cuts at: 1361 1609 1361
Size: 248 3681
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SmaI CCC'GGG
Cuts at: 951 951
Size: 3929
SmlI C'TyrA_G
Cuts at: 456 1084 1999 2491 2753 456
Size: 628 915 492 262 1632
Fragments arranged by size:
1632 915 628 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 982 1482 982
Size: 500 3429
StyI C'CwwG_G
Cuts at: 247 1063 1417 247
Size: 816 354 2759
Fragments arranged by size:
2759 816 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 320 1512 1645 2104 2313 320
Size: 1192 133 459 209 1936
Fragments arranged by size:
1936 1192 459 209 133
TatI w'GTAC_w
Cuts at: 3444 3444
Size: 3929
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 3897
Tth111I GACn'n_nGTC
Cuts at: 1726 1726
Size: 3929
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1424 1424
Size: 3929
XmaI C'CCGG_G
Cuts at: 949 949
Size: 3929
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI