Restriction map for
pF1KB6382
(Circular) (Six-base) MAPSORT of: pF1KB6382.seq Check: 9978 from: 1 to: 4013
pF1KB6382 4013 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1051 1051
Size: 4013
Acc65I G'GTAC_C
Cuts at: 1029 1029
Size: 4013
AclI AA'CG_TT
Cuts at: 3853 3853
Size: 4013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 585 1838 2270 3017 3285 292
Size: 293 1253 432 747 268 1020
Fragments arranged by size:
1253 1020 747 432 293 268
AflIII A'CryG_T
Cuts at: 842 898 1365 2469 3446 842
Size: 56 467 1104 977 1409
Fragments arranged by size:
1409 1104 977 467 56
AgeI A'CCGG_T
Cuts at: 2401 2401
Size: 4013
AlwNI CAG_nnn'CTG
Cuts at: 1318 2885 1318
Size: 1567 2446
ApaLI G'TGCA_C
Cuts at: 2783 2783
Size: 4013
ApoI r'AATT_y
Cuts at: 292 1017 3366 3622 292
Size: 725 2349 256 683
Fragments arranged by size:
2349 725 683 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
AvaI C'yCGr_G
Cuts at: 815 1033 815
Size: 218 3795
BamHI G'GATC_C
Cuts at: 1038 1038
Size: 4013
BanI G'GyrC_C
Cuts at: 1029 1443 1691 1726 1029
Size: 414 248 35 3316
Fragments arranged by size:
3316 414 248 35
BanII G_rGCy'C
Cuts at: 1027 2057 1027
Size: 1030 2983
BbeI G_GCGC'C
Cuts at: 1447 1695 1447
Size: 248 3765
BbsI GAAGACnn'nnnn_
Cuts at: 591 997 591
Size: 406 3607
BciVI GTATCCnnnnn_n'
Cuts at: 370 1911 2672 4013 370
Size: 1541 761 1341 370
Fragments arranged by size:
1541 1341 761 370
BclI T'GATC_A
Cuts at: 1533 1533
Size: 4013
BglII A'GATC_T
Cuts at: 1528 3565 1528
Size: 2037 1976
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4013
Bme1580I G_kGCm'C
Cuts at: 1638 1731 2787 1638
Size: 93 1056 2864
Fragments arranged by size:
2864 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1225 1306 1641 3785 1225
Size: 81 335 2144 1453
Fragments arranged by size:
2144 1453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 748 139
Size: 609 3404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 913 1189 2068 2560 2858 142
Size: 771 276 879 492 298 1297
Fragments arranged by size:
1297 879 771 492 298 276
BsaI GGTCTCn'nnnn_
Cuts at: 550 3717 550
Size: 3167 846
BsaAI yAC'GTr
Cuts at: 901 1366 901
Size: 465 3548
BsaBI GATnn'nnATC
Cuts at: 6 1527 3564 6
Size: 1521 2037 455
Fragments arranged by size:
2037 1521 455
BsaHI Gr'CG_yC
Cuts at: 746 1444 1692 2394 3890 746
Size: 698 248 702 1496 869
Fragments arranged by size:
1496 869 702 698 248
BsaWI w'CCGG_w
Cuts at: 189 1202 1723 2401 2675 2822 3233 189
Size: 1013 521 678 274 147 411 969
Fragments arranged by size:
1013 969 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 713 157
Size: 556 3457
BseYI C'CCAG_C
Cuts at: 1438 2196 2773 3269 1438
Size: 758 577 496 2182
Fragments arranged by size:
2182 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 844
Size: 4013
BsiEI CG_ry'CG
Cuts at: 104 176 1555 1601 2809 104
Size: 72 1379 46 1208 1308
Fragments arranged by size:
1379 1308 1208 72 46
BsiHKAI G_wGCw'C
Cuts at: 816 880 1027 1805 1995 2787 816
Size: 64 147 778 190 792 2042
Fragments arranged by size:
2042 792 778 190 147 64
BsmBI CGTCTCn'nnnn_
Cuts at: 139 139
Size: 4013
Bsp1286I G_dGCh'C
Cuts at: 816 880 1027 1638 1731 1805 1995 2057 2787
Size: 64 147 611 93 74 190 62 730
Cuts at: 2787 816
Size: 2042
Fragments arranged by size:
2042 730 611 190 147 93 74 64
62
BspEI T'CCGG_A
Cuts at: 3233 3233
Size: 4013
BspHI T'CATG_A
Cuts at: 3189 3189
Size: 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 866 1063 1277 1579 1960 866
Size: 197 214 302 381 2919
Fragments arranged by size:
2919 381 302 214 197
BsrBI CCG'CTC
Cuts at: 50 2305 2359 3843 4008 50
Size: 2255 54 1484 165 55
Fragments arranged by size:
2255 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1925 1925
Size: 4013
BsrFI r'CCGG_y
Cuts at: 2011 2192 2401 2011
Size: 181 209 3623
Fragments arranged by size:
3623 209 181
BssSI C'ACGA_G
Cuts at: 2284 2642 2284
Size: 358 3655
BstAPI GCAn_nnn'nTGC
Cuts at: 319 1390 319
Size: 1071 2942
BstBI TT'CG_AA
Cuts at: 2374 2374
Size: 4013
BstYI r'GATC_y
Cuts at: 1 1038 1505 1528 1863 2109 3121 3207 3219
Size: 1037 467 23 335 246 1012 86 12
Cuts at: 3219 3565 1
Size: 346 449
Fragments arranged by size:
1037 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 2144 2307 1963
Size: 181 163 3669
Fragments arranged by size:
3669 181 163
BtsI GCAGTG_nn'
Cuts at: 1256 1256
Size: 4013
DraI TTT'AAA
Cuts at: 1012 1012
Size: 4013
DraIII CAC_nnn'GTG
Cuts at: 319 319
Size: 4013
DrdI GACnn_nn'nnGTC
Cuts at: 631 1719 2577 631
Size: 1088 858 2067
Fragments arranged by size:
2067 1088 858
EaeI y'GGCC_r
Cuts at: 173 1598 1772 2163 2190 3535 173
Size: 1425 174 391 27 1345 651
Fragments arranged by size:
1425 1345 651 391 174 27
EagI C'GGCC_G
Cuts at: 173 1598 173
Size: 1425 2588
EarI CTCTTCn'nnn_
Cuts at: 500 2036 2246 500
Size: 1536 210 2267
Fragments arranged by size:
2267 1536 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2221 2531 2677 3823 2221
Size: 310 146 1146 2411
Fragments arranged by size:
2411 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1025 1025
Size: 4013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 292 585 748 1838 2270 3017 3285 139
Size: 153 293 163 1090 432 747 268 867
Fragments arranged by size:
1090 867 747 432 293 268 163 153
EcoNI CCTnn'n_nnAGG
Cuts at: 118 118
Size: 4013
EcoO109I rG'GnC_Cy
Cuts at: 228 699 1152 228
Size: 471 453 3089
Fragments arranged by size:
3089 471 453
EcoRI G'AATT_C
Cuts at: 1017 1017
Size: 4013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 4013
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4013
HaeII r_GCGC'y
Cuts at: 1447 1695 2717 3696 1447
Size: 248 1022 979 1764
Fragments arranged by size:
1764 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 368 400 1855 1885 1887 1917 368
Size: 32 1455 30 2 30 2464
Fragments arranged by size:
2464 1455 32 30 30 2
HincII GTy'rAC
Cuts at: 1052 1052
Size: 4013
KasI G'GCGC_C
Cuts at: 1443 1691 1443
Size: 248 3765
KpnI G_GTAC'C
Cuts at: 1033 1033
Size: 4013
MluI A'CGCG_T
Cuts at: 3446 3446
Size: 4013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2684 2868 2684
Size: 184 3829
MscI TGG'CCA
Cuts at: 1774 1774
Size: 4013
MslI CAynn'nnrTG
Cuts at: 803 901 2002 2129 803
Size: 98 1101 127 2687
Fragments arranged by size:
2687 1101 127 98
MspA1I CmG'CkG
Cuts at: 751 1124 1438 1798 2811 3056 751
Size: 373 314 360 1013 245 1708
Fragments arranged by size:
1708 1013 373 360 314 245
NaeI GCC'GGC
Cuts at: 2194 2194
Size: 4013
NarI GG'CG_CC
Cuts at: 1444 1692 1444
Size: 248 3765
NgoMIV G'CCGG_C
Cuts at: 2192 2192
Size: 4013
NotI GC'GGCC_GC
Cuts at: 173 173
Size: 4013
NspI r_CATG'y
Cuts at: 453 1066 1566 2473 453
Size: 613 500 907 1993
Fragments arranged by size:
1993 907 613 500
PciI A'CATG_T
Cuts at: 2469 2469
Size: 4013
PfoI T'CCnGG_A
Cuts at: 848 848
Size: 4013
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 3981
PpuMI rG'GwC_Cy
Cuts at: 228 699 228
Size: 471 3542
PstI C_TGCA'G
Cuts at: 1060 1745 1060
Size: 685 3328
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4013
PvuII CAG'CTG
Cuts at: 1438 1798 1438
Size: 360 3653
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4013
SacI G_AGCT'C
Cuts at: 1027 1027
Size: 4013
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4013
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
SbfI CC_TGCA'GG
Cuts at: 1060 1060
Size: 4013
ScaI AGT'ACT
Cuts at: 3530 3530
Size: 4013
SfcI C'TryA_G
Cuts at: 33 1056 1741 2734 2925 33
Size: 1023 685 993 191 1121
Fragments arranged by size:
1121 1023 993 685 191
SfoI GGC'GCC
Cuts at: 1445 1693 1445
Size: 248 3765
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
SmaI CCC'GGG
Cuts at: 1035 1035
Size: 4013
SmlI C'TyrA_G
Cuts at: 157 815 928 1168 2083 2575 2837 157
Size: 658 113 240 915 492 262 1333
Fragments arranged by size:
1333 915 658 492 262 240 113
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4013
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3513
StuI AGG'CCT
Cuts at: 886 886
Size: 4013
StyI C'CwwG_G
Cuts at: 422 1147 1501 422
Size: 725 354 2934
Fragments arranged by size:
2934 725 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 745 1596 1729 2188 2397 745
Size: 851 133 459 209 2361
Fragments arranged by size:
2361 851 459 209 133
TatI w'GTAC_w
Cuts at: 3528 3528
Size: 4013
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 3981
Tth111I GACn'n_nGTC
Cuts at: 586 1810 586
Size: 1224 2789
XbaI T'CTAG_A
Cuts at: 1044 1044
Size: 4013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1508 114
Size: 1394 2619
XhoI C'TCGA_G
Cuts at: 815 815
Size: 4013
XmaI C'CCGG_G
Cuts at: 1033 1033
Size: 4013
XmnI GAAnn'nnTTC
Cuts at: 410 410
Size: 4013
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI