Restriction map for
pF1KB6384
(Circular) (Six-base) MAPSORT of: pF1KB6384.seq Check: 7419 from: 1 to: 4013
pF1KB6384 4013 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 907 907
Size: 4013
AccI GT'mk_AC
Cuts at: 584 1051 584
Size: 467 3546
Acc65I G'GTAC_C
Cuts at: 1029 1029
Size: 4013
AclI AA'CG_TT
Cuts at: 3853 3853
Size: 4013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 1838 2270 3017 3285 314
Size: 1524 432 747 268 1042
Fragments arranged by size:
1524 1042 747 432 268
AflIII A'CryG_T
Cuts at: 1365 2469 3446 1365
Size: 1104 977 1932
Fragments arranged by size:
1932 1104 977
AgeI A'CCGG_T
Cuts at: 2401 2401
Size: 4013
AlwNI CAG_nnn'CTG
Cuts at: 1318 2885 1318
Size: 1567 2446
ApaI G_GGCC'C
Cuts at: 935 935
Size: 4013
ApaLI G'TGCA_C
Cuts at: 2783 2783
Size: 4013
ApoI r'AATT_y
Cuts at: 1017 3366 3622 1017
Size: 2349 256 1408
Fragments arranged by size:
2349 1408 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
AvaI C'yCGr_G
Cuts at: 129 1033 129
Size: 904 3109
BamHI G'GATC_C
Cuts at: 1038 1038
Size: 4013
BanI G'GyrC_C
Cuts at: 1029 1443 1691 1726 1029
Size: 414 248 35 3316
Fragments arranged by size:
3316 414 248 35
BanII G_rGCy'C
Cuts at: 935 1027 2057 935
Size: 92 1030 2891
Fragments arranged by size:
2891 1030 92
BbeI G_GCGC'C
Cuts at: 1447 1695 1447
Size: 248 3765
BbsI GAAGACnn'nnnn_
Cuts at: 419 620 419
Size: 201 3812
BciVI GTATCCnnnnn_n'
Cuts at: 1911 2672 4013 1911
Size: 761 1341 1911
Fragments arranged by size:
1911 1341 761
BclI T'GATC_A
Cuts at: 498 1533 498
Size: 1035 2978
BglI GCCn_nnn'nGGC
Cuts at: 720 720
Size: 4013
BglII A'GATC_T
Cuts at: 623 1528 3565 623
Size: 905 2037 1071
Fragments arranged by size:
2037 1071 905
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4013
Bme1580I G_kGCm'C
Cuts at: 935 1638 1731 2787 935
Size: 703 93 1056 2161
Fragments arranged by size:
2161 1056 703 93
BmrI ACTGGGnnnn_n'
Cuts at: 385 417 711 1225 1306 1641 3785 385
Size: 32 294 514 81 335 2144 613
Fragments arranged by size:
2144 613 514 335 294 81 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 843 2380 843
Size: 1537 2476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 490 178
Size: 312 3701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2068 2560 2858 1189
Size: 879 492 298 2344
Fragments arranged by size:
2344 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 814 3717 814
Size: 2903 1110
BsaAI yAC'GTr
Cuts at: 1366 1366
Size: 4013
BsaBI GATnn'nnATC
Cuts at: 6 883 1527 3564 6
Size: 877 644 2037 455
Fragments arranged by size:
2037 877 644 455
BsaHI Gr'CG_yC
Cuts at: 1444 1692 2394 3890 1444
Size: 248 702 1496 1567
Fragments arranged by size:
1567 1496 702 248
BsaWI w'CCGG_w
Cuts at: 946 1202 1723 2401 2675 2822 3233 946
Size: 256 521 678 274 147 411 1726
Fragments arranged by size:
1726 678 521 411 274 256 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 109 139 109
Size: 30 3983
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 130 969 127
Size: 3 839 3171
Fragments arranged by size:
3171 839 3
BseYI C'CCAG_C
Cuts at: 135 850 1438 2196 2773 3269 135
Size: 715 588 758 577 496 879
Fragments arranged by size:
879 758 715 588 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 928
Size: 4013
BsiEI CG_ry'CG
Cuts at: 104 1555 1601 2809 104
Size: 1451 46 1208 1308
Fragments arranged by size:
1451 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 365 1027 1805 1995 2787 365
Size: 662 778 190 792 1591
Fragments arranged by size:
1591 792 778 662 190
Bsp1286I G_dGCh'C
Cuts at: 365 935 1027 1638 1731 1805 1995 2057 2787
Size: 570 92 611 93 74 190 62 730
Cuts at: 2787 365
Size: 1591
Fragments arranged by size:
1591 730 611 570 190 93 92 74
62
BspEI T'CCGG_A
Cuts at: 3233 3233
Size: 4013
BspHI T'CATG_A
Cuts at: 3189 3189
Size: 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 815 907 1063 1277 1579 1960 815
Size: 92 156 214 302 381 2868
Fragments arranged by size:
2868 381 302 214 156 92
BsrBI CCG'CTC
Cuts at: 50 2305 2359 3843 4008 50
Size: 2255 54 1484 165 55
Fragments arranged by size:
2255 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 564 772 1925 564
Size: 208 1153 2652
Fragments arranged by size:
2652 1153 208
BsrFI r'CCGG_y
Cuts at: 2011 2192 2401 2011
Size: 181 209 3623
Fragments arranged by size:
3623 209 181
BssSI C'ACGA_G
Cuts at: 2284 2642 2284
Size: 358 3655
BstAPI GCAn_nnn'nTGC
Cuts at: 1390 1390
Size: 4013
BstBI TT'CG_AA
Cuts at: 2374 2374
Size: 4013
BstEII G'GTnAC_C
Cuts at: 216 216
Size: 4013
BstYI r'GATC_y
Cuts at: 1 623 846 1038 1505 1528 1863 2109 3121
Size: 622 223 192 467 23 335 246 1012
Cuts at: 3121 3207 3219 3565 1
Size: 86 12 346 449
Fragments arranged by size:
1012 622 467 449 346 335 246 223
192 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 936 936
Size: 4013
BtgI C'CryG_G
Cuts at: 107 107
Size: 4013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 2144 2307 1963
Size: 181 163 3669
Fragments arranged by size:
3669 181 163
BtsI GCAGTG_nn'
Cuts at: 537 570 1256 537
Size: 33 686 3294
Fragments arranged by size:
3294 686 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 546 581 546
Size: 35 3978
DraI TTT'AAA
Cuts at: 1012 1012
Size: 4013
DrdI GACnn_nn'nnGTC
Cuts at: 947 1719 2577 947
Size: 772 858 2383
Fragments arranged by size:
2383 858 772
EaeI y'GGCC_r
Cuts at: 921 1598 1772 2163 2190 3535 921
Size: 677 174 391 27 1345 1399
Fragments arranged by size:
1399 1345 677 391 174 27
EagI C'GGCC_G
Cuts at: 1598 1598
Size: 4013
EarI CTCTTCn'nnn_
Cuts at: 812 2036 2246 812
Size: 1224 210 2579
Fragments arranged by size:
2579 1224 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2221 2531 2677 3823 2221
Size: 310 146 1146 2411
Fragments arranged by size:
2411 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1025 1025
Size: 4013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 314 490 1838 2270 3017 3285 178
Size: 136 176 1348 432 747 268 906
Fragments arranged by size:
1348 906 747 432 268 176 136
EcoO109I rG'GnC_Cy
Cuts at: 901 931 932 940 1152 901
Size: 30 1 8 212 3762
Fragments arranged by size:
3762 212 30 8 1
EcoRI G'AATT_C
Cuts at: 1017 1017
Size: 4013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 534 595 2032 534
Size: 61 1437 2515
Fragments arranged by size:
2515 1437 61
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4013
HaeII r_GCGC'y
Cuts at: 1447 1695 2717 3696 1447
Size: 248 1022 979 1764
Fragments arranged by size:
1764 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 109 138 141 1855 1885 1887 1917 106
Size: 3 29 3 1714 30 2 30 2202
Fragments arranged by size:
2202 1714 30 30 29 3 3 2
HincII GTy'rAC
Cuts at: 1052 1052
Size: 4013
KasI G'GCGC_C
Cuts at: 1443 1691 1443
Size: 248 3765
KpnI G_GTAC'C
Cuts at: 1033 1033
Size: 4013
MluI A'CGCG_T
Cuts at: 3446 3446
Size: 4013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2684 2868 2684
Size: 184 3829
MscI TGG'CCA
Cuts at: 923 1774 923
Size: 851 3162
MslI CAynn'nnrTG
Cuts at: 2002 2129 2002
Size: 127 3886
MspA1I CmG'CkG
Cuts at: 1124 1438 1798 2811 3056 1124
Size: 314 360 1013 245 2081
Fragments arranged by size:
2081 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2194 2194
Size: 4013
NarI GG'CG_CC
Cuts at: 1444 1692 1444
Size: 248 3765
NcoI C'CATG_G
Cuts at: 107 107
Size: 4013
NdeI CA'TA_TG
Cuts at: 251 251
Size: 4013
NgoMIV G'CCGG_C
Cuts at: 2192 2192
Size: 4013
NspI r_CATG'y
Cuts at: 780 1066 1566 2473 780
Size: 286 500 907 2320
Fragments arranged by size:
2320 907 500 286
PasI CC'CwG_GG
Cuts at: 171 171
Size: 4013
PciI A'CATG_T
Cuts at: 2469 2469
Size: 4013
PflMI CCAn_nnn'nTGG
Cuts at: 767 767
Size: 4013
PfoI T'CCnGG_A
Cuts at: 632 632
Size: 4013
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 613 645 3152 3184 613
Size: 32 2507 32 1442
Fragments arranged by size:
2507 1442 32 32
PpuMI rG'GwC_Cy
Cuts at: 940 940
Size: 4013
PspOMI G'GGCC_C
Cuts at: 931 931
Size: 4013
PstI C_TGCA'G
Cuts at: 145 918 1060 1745 145
Size: 773 142 685 2413
Fragments arranged by size:
2413 773 685 142
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4013
PvuII CAG'CTG
Cuts at: 1438 1798 1438
Size: 360 3653
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4013
SacI G_AGCT'C
Cuts at: 1027 1027
Size: 4013
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4013
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
SbfI CC_TGCA'GG
Cuts at: 918 1060 918
Size: 142 3871
ScaI AGT'ACT
Cuts at: 3530 3530
Size: 4013
SfcI C'TryA_G
Cuts at: 33 141 914 1056 1741 2734 2925 33
Size: 108 773 142 685 993 191 1121
Fragments arranged by size:
1121 993 773 685 191 142 108
SfoI GGC'GCC
Cuts at: 1445 1693 1445
Size: 248 3765
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
SmaI CCC'GGG
Cuts at: 131 1035 131
Size: 904 3109
SmlI C'TyrA_G
Cuts at: 1168 2083 2575 2837 1168
Size: 915 492 262 2344
Fragments arranged by size:
2344 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4013
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3513
StyI C'CwwG_G
Cuts at: 107 1147 1501 107
Size: 1040 354 2619
Fragments arranged by size:
2619 1040 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 436 468 715 838 1596 1729 2188 2397 436
Size: 32 247 123 758 133 459 209 2052
Fragments arranged by size:
2052 758 459 247 209 133 123 32
TatI w'GTAC_w
Cuts at: 3528 3528
Size: 4013
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 3981
Tth111I GACn'n_nGTC
Cuts at: 1810 1810
Size: 4013
XbaI T'CTAG_A
Cuts at: 1044 1044
Size: 4013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1508 1508
Size: 4013
XmaI C'CCGG_G
Cuts at: 129 1033 129
Size: 904 3109
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI