Restriction map for
pF1KB6386
(Circular) (Six-base) MAPSORT of: pF1KB6386.seq Check: 4815 from: 1 to: 4016
pF1KB6386 4016 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 556 556
Size: 4016
AccI GT'mk_AC
Cuts at: 766 1054 766
Size: 288 3728
Acc65I G'GTAC_C
Cuts at: 1032 1032
Size: 4016
AclI AA'CG_TT
Cuts at: 3856 3856
Size: 4016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1841 2273 3020 3288 1841
Size: 432 747 268 2569
Fragments arranged by size:
2569 747 432 268
AfeI AGC'GCT
Cuts at: 238 238
Size: 4016
AflIII A'CryG_T
Cuts at: 1368 2472 3449 1368
Size: 1104 977 1935
Fragments arranged by size:
1935 1104 977
AgeI A'CCGG_T
Cuts at: 2404 2404
Size: 4016
AlwNI CAG_nnn'CTG
Cuts at: 562 1321 2888 562
Size: 759 1567 1690
Fragments arranged by size:
1690 1567 759
ApaI G_GGCC'C
Cuts at: 549 549
Size: 4016
ApaLI G'TGCA_C
Cuts at: 804 2786 804
Size: 1982 2034
ApoI r'AATT_y
Cuts at: 933 1020 3369 3625 933
Size: 87 2349 256 1324
Fragments arranged by size:
2349 1324 256 87
AseI AT'TA_AT
Cuts at: 20 20
Size: 4016
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
AvaI C'yCGr_G
Cuts at: 1036 1036
Size: 4016
BamHI G'GATC_C
Cuts at: 1041 1041
Size: 4016
BanI G'GyrC_C
Cuts at: 886 1032 1446 1694 1729 886
Size: 146 414 248 35 3173
Fragments arranged by size:
3173 414 248 146 35
BanII G_rGCy'C
Cuts at: 405 549 1030 2060 405
Size: 144 481 1030 2361
Fragments arranged by size:
2361 1030 481 144
BbeI G_GCGC'C
Cuts at: 1450 1698 1450
Size: 248 3768
BbsI GAAGACnn'nnnn_
Cuts at: 869 869
Size: 4016
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 795 829 795
Size: 34 3982
BciVI GTATCCnnnnn_n'
Cuts at: 1914 2675 4016 1914
Size: 761 1341 1914
Fragments arranged by size:
1914 1341 761
BclI T'GATC_A
Cuts at: 1536 1536
Size: 4016
BglII A'GATC_T
Cuts at: 1531 3568 1531
Size: 2037 1979
BlpI GC'TnA_GC
Cuts at: 1128 1128
Size: 4016
Bme1580I G_kGCm'C
Cuts at: 549 808 1641 1734 2790 549
Size: 259 833 93 1056 1775
Fragments arranged by size:
1775 1056 833 259 93
BmrI ACTGGGnnnn_n'
Cuts at: 185 1228 1309 1644 3788 185
Size: 1043 81 335 2144 413
Fragments arranged by size:
2144 1043 413 335 81
BmtI G_CTAG'C
Cuts at: 727 727
Size: 4016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 805 868 958 520
Size: 285 63 90 3578
Fragments arranged by size:
3578 285 90 63
Bpu10I CC'TnA_GC
Cuts at: 404 404
Size: 4016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 2071 2563 2861 1192
Size: 879 492 298 2347
Fragments arranged by size:
2347 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 522 3720 522
Size: 3198 818
BsaAI yAC'GTr
Cuts at: 150 741 919 1369 150
Size: 591 178 450 2797
Fragments arranged by size:
2797 591 450 178
BsaBI GATnn'nnATC
Cuts at: 6 1530 3567 6
Size: 1524 2037 455
Fragments arranged by size:
2037 1524 455
BsaHI Gr'CG_yC
Cuts at: 1447 1695 2397 3893 1447
Size: 248 702 1496 1570
Fragments arranged by size:
1570 1496 702 248
BsaWI w'CCGG_w
Cuts at: 536 1205 1726 2404 2678 2825 3236 536
Size: 669 521 678 274 147 411 1316
Fragments arranged by size:
1316 678 669 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 913 322
Size: 591 3425
BseYI C'CCAG_C
Cuts at: 939 1441 2199 2776 3272 939
Size: 502 758 577 496 1683
Fragments arranged by size:
1683 758 577 502 496
BsiEI CG_ry'CG
Cuts at: 104 1558 1604 2812 104
Size: 1454 46 1208 1308
Fragments arranged by size:
1454 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 258 405 808 824 1030 1808 1998 2790 258
Size: 147 403 16 206 778 190 792 1484
Fragments arranged by size:
1484 792 778 403 206 190 147 16
Bsp1286I G_dGCh'C
Cuts at: 258 405 549 808 824 1030 1641 1734 1808
Size: 147 144 259 16 206 611 93 74
Cuts at: 1808 1998 2060 2790 258
Size: 190 62 730 1484
Fragments arranged by size:
1484 730 611 259 206 190 147 144
93 74 62 16
BspEI T'CCGG_A
Cuts at: 3236 3236
Size: 4016
BspHI T'CATG_A
Cuts at: 3192 3192
Size: 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 556 1066 1280 1582 1963 556
Size: 510 214 302 381 2609
Fragments arranged by size:
2609 510 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2308 2362 3846 4011 50
Size: 2258 54 1484 165 55
Fragments arranged by size:
2258 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 606 1928 606
Size: 1322 2694
BsrFI r'CCGG_y
Cuts at: 245 2014 2195 2404 245
Size: 1769 181 209 1857
Fragments arranged by size:
1857 1769 209 181
BsrGI T'GTAC_A
Cuts at: 528 528
Size: 4016
BssSI C'ACGA_G
Cuts at: 2287 2645 2287
Size: 358 3658
BstAPI GCAn_nnn'nTGC
Cuts at: 562 733 1393 562
Size: 171 660 3185
Fragments arranged by size:
3185 660 171
BstBI TT'CG_AA
Cuts at: 815 2377 815
Size: 1562 2454
BstXI CCAn_nnnn'nTGG
Cuts at: 270 270
Size: 4016
BstYI r'GATC_y
Cuts at: 1 503 963 1041 1508 1531 1866 2112 3124
Size: 502 460 78 467 23 335 246 1012
Cuts at: 3124 3210 3222 3568 1
Size: 86 12 346 449
Fragments arranged by size:
1012 502 467 460 449 346 335 246
86 78 23 12
BstZ17I GTA'TAC
Cuts at: 767 767
Size: 4016
BtgI C'CryG_G
Cuts at: 107 269 107
Size: 162 3854
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 567 1966 2147 2310 567
Size: 1399 181 163 2273
Fragments arranged by size:
2273 1399 181 163
BtsI GCAGTG_nn'
Cuts at: 986 1259 986
Size: 273 3743
DraI TTT'AAA
Cuts at: 1015 1015
Size: 4016
DrdI GACnn_nn'nnGTC
Cuts at: 1722 2580 1722
Size: 858 3158
EaeI y'GGCC_r
Cuts at: 206 1601 1775 2166 2193 3538 206
Size: 1395 174 391 27 1345 684
Fragments arranged by size:
1395 1345 684 391 174 27
EagI C'GGCC_G
Cuts at: 1601 1601
Size: 4016
EarI CTCTTCn'nnn_
Cuts at: 667 812 2039 2249 667
Size: 145 1227 210 2434
Fragments arranged by size:
2434 1227 210 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 289 2224 2534 2680 3826 289
Size: 1935 310 146 1146 479
Fragments arranged by size:
1935 1146 479 310 146
EcoICRI GAG'CTC
Cuts at: 403 1028 403
Size: 625 3391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 805 868 958 1841 2273 3020 3288 520
Size: 285 63 90 883 432 747 268 1248
Fragments arranged by size:
1248 883 747 432 285 268 90 63
EcoO109I rG'GnC_Cy
Cuts at: 219 546 852 1155 219
Size: 327 306 303 3080
Fragments arranged by size:
3080 327 306 303
EcoRI G'AATT_C
Cuts at: 1020 1020
Size: 4016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 672 2035 672
Size: 1363 2653
FspI TGC'GCA
Cuts at: 1797 1797
Size: 4016
HaeII r_GCGC'y
Cuts at: 240 1450 1698 2720 3699 240
Size: 1210 248 1022 979 557
Fragments arranged by size:
1210 1022 979 557 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 792 824 1858 1888 1890 1920 792
Size: 32 1034 30 2 30 2888
Fragments arranged by size:
2888 1034 32 30 30 2
HincII GTy'rAC
Cuts at: 1055 1055
Size: 4016
KasI G'GCGC_C
Cuts at: 1446 1694 1446
Size: 248 3768
KpnI G_GTAC'C
Cuts at: 1036 1036
Size: 4016
MluI A'CGCG_T
Cuts at: 3449 3449
Size: 4016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 287 2687 2871 287
Size: 2400 184 1432
Fragments arranged by size:
2400 1432 184
MscI TGG'CCA
Cuts at: 1777 1777
Size: 4016
MslI CAynn'nnrTG
Cuts at: 2005 2132 2005
Size: 127 3889
MspA1I CmG'CkG
Cuts at: 471 642 1127 1441 1801 2814 3059 471
Size: 171 485 314 360 1013 245 1428
Fragments arranged by size:
1428 1013 485 360 314 245 171
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 4016
NarI GG'CG_CC
Cuts at: 1447 1695 1447
Size: 248 3768
NcoI C'CATG_G
Cuts at: 107 269 107
Size: 162 3854
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 4016
NheI G'CTAG_C
Cuts at: 723 723
Size: 4016
NspI r_CATG'y
Cuts at: 1069 1569 2476 1069
Size: 500 907 2609
Fragments arranged by size:
2609 907 500
PciI A'CATG_T
Cuts at: 2472 2472
Size: 4016
PmeI GTTT'AAAC
Cuts at: 1015 1015
Size: 4016
PmlI CAC'GTG
Cuts at: 150 150
Size: 4016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3984
PpuMI rG'GwC_Cy
Cuts at: 852 852
Size: 4016
PspOMI G'GGCC_C
Cuts at: 545 545
Size: 4016
PstI C_TGCA'G
Cuts at: 567 1063 1748 567
Size: 496 685 2835
Fragments arranged by size:
2835 685 496
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4016
PvuII CAG'CTG
Cuts at: 642 1441 1801 642
Size: 799 360 2857
Fragments arranged by size:
2857 799 360
RsrII CG'GwC_CG
Cuts at: 2211 2211
Size: 4016
SacI G_AGCT'C
Cuts at: 405 1030 405
Size: 625 3391
SalI G'TCGA_C
Cuts at: 1053 1053
Size: 4016
SapI GCTCTTCn'nnn_
Cuts at: 812 2039 2249 812
Size: 1227 210 2579
Fragments arranged by size:
2579 1227 210
SbfI CC_TGCA'GG
Cuts at: 567 1063 567
Size: 496 3520
ScaI AGT'ACT
Cuts at: 3533 3533
Size: 4016
SfcI C'TryA_G
Cuts at: 33 563 1059 1744 2737 2928 33
Size: 530 496 685 993 191 1121
Fragments arranged by size:
1121 993 685 530 496 191
SfoI GGC'GCC
Cuts at: 1448 1696 1448
Size: 248 3768
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
SmaI CCC'GGG
Cuts at: 1038 1038
Size: 4016
SmlI C'TyrA_G
Cuts at: 1171 2086 2578 2840 1171
Size: 915 492 262 2347
Fragments arranged by size:
2347 915 492 262
SnaBI TAC'GTA
Cuts at: 919 919
Size: 4016
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4016
SphI G_CATG'C
Cuts at: 1069 1569 1069
Size: 500 3516
StyI C'CwwG_G
Cuts at: 107 269 1150 1504 107
Size: 162 881 354 2619
Fragments arranged by size:
2619 881 354 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 839 1599 1732 2191 2400 839
Size: 760 133 459 209 2455
Fragments arranged by size:
2455 760 459 209 133
TatI w'GTAC_w
Cuts at: 528 3531 528
Size: 3003 1013
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 458 490 3533 3565 458
Size: 32 3043 32 909
Fragments arranged by size:
3043 909 32 32
Tth111I GACn'n_nGTC
Cuts at: 1813 1813
Size: 4016
XbaI T'CTAG_A
Cuts at: 1047 1047
Size: 4016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1511 1511
Size: 4016
XmaI C'CCGG_G
Cuts at: 1036 1036
Size: 4016
XmnI GAAnn'nnTTC
Cuts at: 934 934
Size: 4016
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BsaXI BsgI BsiWI
BsmI BsmBI BssHII BstEII Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PflMI PfoI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI ZraI