Restriction map for
pF1KB6387
(Circular) (Six-base) MAPSORT of: pF1KB6387.seq Check: 2797 from: 1 to: 3938
pF1KB6387 3938 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AatII G_ACGT'C
Cuts at: 892 892
Size: 3938
AccI GT'mk_AC
Cuts at: 976 976
Size: 3938
Acc65I G'GTAC_C
Cuts at: 954 954
Size: 3938
AclI AA'CG_TT
Cuts at: 3778 3778
Size: 3938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1763 2195 2942 3210 1763
Size: 432 747 268 2491
Fragments arranged by size:
2491 747 432 268
AflIII A'CryG_T
Cuts at: 1290 2394 3371 1290
Size: 1104 977 1857
Fragments arranged by size:
1857 1104 977
AgeI A'CCGG_T
Cuts at: 2326 2326
Size: 3938
AlwNI CAG_nnn'CTG
Cuts at: 1243 2810 1243
Size: 1567 2371
ApaLI G'TGCA_C
Cuts at: 2708 2708
Size: 3938
ApoI r'AATT_y
Cuts at: 942 3291 3547 942
Size: 2349 256 1333
Fragments arranged by size:
2349 1333 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3938
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
AvaI C'yCGr_G
Cuts at: 195 958 195
Size: 763 3175
BamHI G'GATC_C
Cuts at: 963 963
Size: 3938
BanI G'GyrC_C
Cuts at: 954 1368 1616 1651 954
Size: 414 248 35 3241
Fragments arranged by size:
3241 414 248 35
BanII G_rGCy'C
Cuts at: 380 952 1982 380
Size: 572 1030 2336
Fragments arranged by size:
2336 1030 572
BbeI G_GCGC'C
Cuts at: 1372 1620 1372
Size: 248 3690
BciVI GTATCCnnnnn_n'
Cuts at: 1836 2597 3938 1836
Size: 761 1341 1836
Fragments arranged by size:
1836 1341 761
BclI T'GATC_A
Cuts at: 1458 1458
Size: 3938
BglII A'GATC_T
Cuts at: 588 908 1453 3490 588
Size: 320 545 2037 1036
Fragments arranged by size:
2037 1036 545 320
BlpI GC'TnA_GC
Cuts at: 1050 1050
Size: 3938
Bme1580I G_kGCm'C
Cuts at: 1563 1656 2712 1563
Size: 93 1056 2789
Fragments arranged by size:
2789 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 417 1150 1231 1566 3710 417
Size: 733 81 335 2144 645
Fragments arranged by size:
2144 733 645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 3938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 728 808 900 503
Size: 225 80 92 3541
Fragments arranged by size:
3541 225 92 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1993 2485 2783 1114
Size: 879 492 298 2269
Fragments arranged by size:
2269 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 443 3642 443
Size: 3199 739
BsaAI yAC'GTr
Cuts at: 1291 1291
Size: 3938
BsaBI GATnn'nnATC
Cuts at: 6 1452 3489 6
Size: 1446 2037 455
Fragments arranged by size:
2037 1446 455
BsaHI Gr'CG_yC
Cuts at: 889 1369 1617 2319 3815 889
Size: 480 248 702 1496 1012
Fragments arranged by size:
1496 1012 702 480 248
BsaWI w'CCGG_w
Cuts at: 1127 1648 2326 2600 2747 3158 1127
Size: 521 678 274 147 411 1907
Fragments arranged by size:
1907 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 247 277 440 470 247
Size: 30 163 30 3715
Fragments arranged by size:
3715 163 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 217
Size: 3938
BseYI C'CCAG_C
Cuts at: 1363 2121 2698 3194 1363
Size: 758 577 496 2107
Fragments arranged by size:
2107 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 269 598 149
Size: 120 329 3489
Fragments arranged by size:
3489 329 120
BsiEI CG_ry'CG
Cuts at: 104 262 360 1480 1526 2734 104
Size: 158 98 1120 46 1208 1308
Fragments arranged by size:
1308 1208 1120 158 98 46
BsiHKAI G_wGCw'C
Cuts at: 194 289 952 1730 1920 2712 194
Size: 95 663 778 190 792 1420
Fragments arranged by size:
1420 792 778 663 190 95
BsmI GAATG_Cn'
Cuts at: 279 279
Size: 3938
Bsp1286I G_dGCh'C
Cuts at: 194 289 380 952 1563 1656 1730 1920 1982
Size: 95 91 572 611 93 74 190 62
Cuts at: 1982 2712 194
Size: 730 1420
Fragments arranged by size:
1420 730 611 572 190 95 93 91
74 62
BspEI T'CCGG_A
Cuts at: 3158 3158
Size: 3938
BspHI T'CATG_A
Cuts at: 3114 3114
Size: 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 126 570 988 1202 1504 1885 126
Size: 444 418 214 302 381 2179
Fragments arranged by size:
2179 444 418 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2230 2284 3768 3933 50
Size: 2180 54 1484 165 55
Fragments arranged by size:
2180 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1850 1850
Size: 3938
BsrFI r'CCGG_y
Cuts at: 1936 2117 2326 1936
Size: 181 209 3548
Fragments arranged by size:
3548 209 181
BssHII G'CGCG_C
Cuts at: 157 159 157
Size: 2 3936
BssSI C'ACGA_G
Cuts at: 2209 2567 2209
Size: 358 3580
BstAPI GCAn_nnn'nTGC
Cuts at: 1315 1315
Size: 3938
BstBI TT'CG_AA
Cuts at: 2299 2299
Size: 3938
BstEII G'GTnAC_C
Cuts at: 345 345
Size: 3938
BstXI CCAn_nnnn'nTGG
Cuts at: 460 675 460
Size: 215 3723
BstYI r'GATC_y
Cuts at: 1 588 908 963 1430 1453 1788 2034 3046
Size: 587 320 55 467 23 335 246 1012
Cuts at: 3046 3132 3144 3490 1
Size: 86 12 346 449
Fragments arranged by size:
1012 587 467 449 346 335 320 246
86 55 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3938
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1888 2069 2232 1888
Size: 181 163 3594
Fragments arranged by size:
3594 181 163
BtsI GCAGTG_nn'
Cuts at: 257 390 1181 257
Size: 133 791 3014
Fragments arranged by size:
3014 791 133
DraI TTT'AAA
Cuts at: 708 937 708
Size: 229 3709
DraIII CAC_nnn'GTG
Cuts at: 353 353
Size: 3938
DrdI GACnn_nn'nnGTC
Cuts at: 695 1644 2502 695
Size: 949 858 2131
Fragments arranged by size:
2131 949 858
EaeI y'GGCC_r
Cuts at: 110 357 600 1523 1697 2088 2115 3460 110
Size: 247 243 923 174 391 27 1345 588
Fragments arranged by size:
1345 923 588 391 247 243 174 27
EagI C'GGCC_G
Cuts at: 357 1523 357
Size: 1166 2772
EarI CTCTTCn'nnn_
Cuts at: 1961 2171 1961
Size: 210 3728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2146 2456 2602 3748 2146
Size: 310 146 1146 2336
Fragments arranged by size:
2336 1146 310 146
EcoICRI GAG'CTC
Cuts at: 950 950
Size: 3938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 728 808 900 1763 2195 2942 3210 503
Size: 225 80 92 863 432 747 268 1231
Fragments arranged by size:
1231 863 747 432 268 225 92 80
EcoO109I rG'GnC_Cy
Cuts at: 238 1077 238
Size: 839 3099
EcoRI G'AATT_C
Cuts at: 942 942
Size: 3938
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1957 1957
Size: 3938
FspI TGC'GCA
Cuts at: 1719 1719
Size: 3938
HaeII r_GCGC'y
Cuts at: 327 1372 1620 2642 3621 327
Size: 1045 248 1022 979 644
Fragments arranged by size:
1045 1022 979 644 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 578 610 879 911 1780 1810 1812 1842 578
Size: 32 269 32 869 30 2 30 2674
Fragments arranged by size:
2674 869 269 32 32 30 30 2
HincII GTy'rAC
Cuts at: 977 977
Size: 3938
HindIII A'AGCT_T
Cuts at: 872 872
Size: 3938
KasI G'GCGC_C
Cuts at: 1368 1616 1368
Size: 248 3690
KpnI G_GTAC'C
Cuts at: 958 958
Size: 3938
MluI A'CGCG_T
Cuts at: 3371 3371
Size: 3938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 486 564 2609 2793 486
Size: 78 2045 184 1631
Fragments arranged by size:
2045 1631 184 78
MscI TGG'CCA
Cuts at: 602 1699 602
Size: 1097 2841
MslI CAynn'nnrTG
Cuts at: 1927 2054 1927
Size: 127 3811
MspA1I CmG'CkG
Cuts at: 392 656 1049 1363 1723 2736 2981 392
Size: 264 393 314 360 1013 245 1349
Fragments arranged by size:
1349 1013 393 360 314 264 245
NaeI GCC'GGC
Cuts at: 2119 2119
Size: 3938
NarI GG'CG_CC
Cuts at: 1369 1617 1369
Size: 248 3690
NcoI C'CATG_G
Cuts at: 107 107
Size: 3938
NgoMIV G'CCGG_C
Cuts at: 2117 2117
Size: 3938
NotI GC'GGCC_GC
Cuts at: 357 357
Size: 3938
NspI r_CATG'y
Cuts at: 991 1491 2398 991
Size: 500 907 2531
Fragments arranged by size:
2531 907 500
PasI CC'CwG_GG
Cuts at: 241 241
Size: 3938
PciI A'CATG_T
Cuts at: 2394 2394
Size: 3938
PflMI CCAn_nnn'nTGG
Cuts at: 609 609
Size: 3938
PmeI GTTT'AAAC
Cuts at: 937 937
Size: 3938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3077 3109 3077
Size: 32 3906
PstI C_TGCA'G
Cuts at: 985 1670 985
Size: 685 3253
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3938
PvuII CAG'CTG
Cuts at: 656 1363 1723 656
Size: 707 360 2871
Fragments arranged by size:
2871 707 360
RsrII CG'GwC_CG
Cuts at: 310 2133 310
Size: 1823 2115
SacI G_AGCT'C
Cuts at: 952 952
Size: 3938
SalI G'TCGA_C
Cuts at: 975 975
Size: 3938
SapI GCTCTTCn'nnn_
Cuts at: 1961 2171 1961
Size: 210 3728
SbfI CC_TGCA'GG
Cuts at: 985 985
Size: 3938
ScaI AGT'ACT
Cuts at: 176 3455 176
Size: 3279 659
SfcI C'TryA_G
Cuts at: 33 981 1666 2659 2850 33
Size: 948 685 993 191 1121
Fragments arranged by size:
1121 993 948 685 191
SfoI GGC'GCC
Cuts at: 1370 1618 1370
Size: 248 3690
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
SmaI CCC'GGG
Cuts at: 197 960 197
Size: 763 3175
SmlI C'TyrA_G
Cuts at: 1093 2008 2500 2762 1093
Size: 915 492 262 2269
Fragments arranged by size:
2269 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3938
SphI G_CATG'C
Cuts at: 991 1491 991
Size: 500 3438
StyI C'CwwG_G
Cuts at: 107 1072 1426 107
Size: 965 354 2619
Fragments arranged by size:
2619 965 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 399 667 1521 1654 2113 2322 399
Size: 268 854 133 459 209 2015
Fragments arranged by size:
2015 854 459 268 209 133
TatI w'GTAC_w
Cuts at: 174 3453 174
Size: 3279 659
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 440 472 3455 3487 440
Size: 32 2983 32 891
Fragments arranged by size:
2983 891 32 32
Tth111I GACn'n_nGTC
Cuts at: 1735 1735
Size: 3938
XbaI T'CTAG_A
Cuts at: 969 969
Size: 3938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1433 1433
Size: 3938
XmaI C'CCGG_G
Cuts at: 195 958 195
Size: 763 3175
ZraI GAC'GTC
Cuts at: 890 890
Size: 3938
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NruI NsiI PacI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI