Restriction map for
pF1KB6391
(Circular) (Six-base) MAPSORT of: pF1KB6391.seq Check: 3208 from: 1 to: 3941
pF1KB6391 3941 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 979 979
Size: 3941
Acc65I G'GTAC_C
Cuts at: 957 957
Size: 3941
AclI AA'CG_TT
Cuts at: 3781 3781
Size: 3941
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 835 1766 2198 2945 3213 626
Size: 209 931 432 747 268 1354
Fragments arranged by size:
1354 931 747 432 268 209
AflIII A'CryG_T
Cuts at: 1293 2397 3374 1293
Size: 1104 977 1860
Fragments arranged by size:
1860 1104 977
AgeI A'CCGG_T
Cuts at: 2329 2329
Size: 3941
AleI CACnn'nnGTG
Cuts at: 369 369
Size: 3941
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 329 361 329
Size: 32 3909
AlwNI CAG_nnn'CTG
Cuts at: 1246 2813 1246
Size: 1567 2374
ApaLI G'TGCA_C
Cuts at: 2711 2711
Size: 3941
ApoI r'AATT_y
Cuts at: 199 301 450 945 3294 3550 199
Size: 102 149 495 2349 256 590
Fragments arranged by size:
2349 590 495 256 149 102
AseI AT'TA_AT
Cuts at: 20 20
Size: 3941
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
AvaI C'yCGr_G
Cuts at: 961 961
Size: 3941
BamHI G'GATC_C
Cuts at: 966 966
Size: 3941
BanI G'GyrC_C
Cuts at: 957 1371 1619 1654 957
Size: 414 248 35 3244
Fragments arranged by size:
3244 414 248 35
BanII G_rGCy'C
Cuts at: 955 1985 955
Size: 1030 2911
BbeI G_GCGC'C
Cuts at: 1375 1623 1375
Size: 248 3693
BbsI GAAGACnn'nnnn_
Cuts at: 802 802
Size: 3941
BciVI GTATCCnnnnn_n'
Cuts at: 331 1839 2600 3941 331
Size: 1508 761 1341 331
Fragments arranged by size:
1508 1341 761 331
BclI T'GATC_A
Cuts at: 1461 1461
Size: 3941
BfrBI ATG'CAT
Cuts at: 482 482
Size: 3941
BglI GCCn_nnn'nGGC
Cuts at: 112 418 112
Size: 306 3635
BglII A'GATC_T
Cuts at: 1456 3493 1456
Size: 2037 1904
BlpI GC'TnA_GC
Cuts at: 1053 1053
Size: 3941
Bme1580I G_kGCm'C
Cuts at: 1566 1659 2715 1566
Size: 93 1056 2792
Fragments arranged by size:
2792 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1153 1234 1569 3713 1153
Size: 81 335 2144 1381
Fragments arranged by size:
2144 1381 335 81
BmtI G_CTAG'C
Cuts at: 425 580 425
Size: 155 3786
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 3941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 422 473 761 860 160
Size: 262 51 288 99 3241
Fragments arranged by size:
3241 288 262 99 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1996 2488 2786 1117
Size: 879 492 298 2272
Fragments arranged by size:
2272 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3645 3645
Size: 3941
BsaAI yAC'GTr
Cuts at: 1294 1294
Size: 3941
BsaBI GATnn'nnATC
Cuts at: 6 1455 3492 6
Size: 1449 2037 455
Fragments arranged by size:
2037 1449 455
BsaHI Gr'CG_yC
Cuts at: 1372 1620 2322 3818 1372
Size: 248 702 1496 1495
Fragments arranged by size:
1496 1495 702 248
BsaWI w'CCGG_w
Cuts at: 386 1130 1651 2329 2603 2750 3161 386
Size: 744 521 678 274 147 411 1166
Fragments arranged by size:
1166 744 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 760 760
Size: 3941
BseYI C'CCAG_C
Cuts at: 115 1366 2124 2701 3197 115
Size: 1251 758 577 496 859
Fragments arranged by size:
1251 859 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 546
Size: 3941
BsiEI CG_ry'CG
Cuts at: 104 1483 1529 2737 104
Size: 1379 46 1208 1308
Fragments arranged by size:
1379 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 955 1733 1923 2715 955
Size: 778 190 792 2181
Fragments arranged by size:
2181 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 955 1566 1659 1733 1923 1985 2715 955
Size: 611 93 74 190 62 730 2181
Fragments arranged by size:
2181 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3161 3161
Size: 3941
BspHI T'CATG_A
Cuts at: 3117 3117
Size: 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 991 1205 1507 1888 991
Size: 214 302 381 3044
Fragments arranged by size:
3044 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2233 2287 3771 3936 50
Size: 2183 54 1484 165 55
Fragments arranged by size:
2183 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 431 1853 431
Size: 1422 2519
BsrFI r'CCGG_y
Cuts at: 1939 2120 2329 1939
Size: 181 209 3551
Fragments arranged by size:
3551 209 181
BsrGI T'GTAC_A
Cuts at: 594 594
Size: 3941
BssSI C'ACGA_G
Cuts at: 2212 2570 2212
Size: 358 3583
BstAPI GCAn_nnn'nTGC
Cuts at: 304 1318 304
Size: 1014 2927
BstBI TT'CG_AA
Cuts at: 2302 2302
Size: 3941
BstYI r'GATC_y
Cuts at: 1 357 966 1433 1456 1791 2037 3049 3135
Size: 356 609 467 23 335 246 1012 86
Cuts at: 3135 3147 3493 1
Size: 12 346 449
Fragments arranged by size:
1012 609 467 449 356 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 128 107
Size: 21 3920
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1891 2072 2235 1891
Size: 181 163 3597
Fragments arranged by size:
3597 181 163
BtsI GCAGTG_nn'
Cuts at: 1184 1184
Size: 3941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 804 839 804
Size: 35 3906
DraI TTT'AAA
Cuts at: 587 650 940 587
Size: 63 290 3588
Fragments arranged by size:
3588 290 63
DrdI GACnn_nn'nnGTC
Cuts at: 1647 2505 1647
Size: 858 3083
EaeI y'GGCC_r
Cuts at: 1526 1700 2091 2118 3463 1526
Size: 174 391 27 1345 2004
Fragments arranged by size:
2004 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1526 1526
Size: 3941
EarI CTCTTCn'nnn_
Cuts at: 180 401 1964 2174 180
Size: 221 1563 210 1947
Fragments arranged by size:
1947 1563 221 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2149 2459 2605 3751 2149
Size: 310 146 1146 2339
Fragments arranged by size:
2339 1146 310 146
EcoICRI GAG'CTC
Cuts at: 953 953
Size: 3941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 422 473 626 761 835 860 1766 2198
Size: 262 51 153 135 74 25 906 432
Cuts at: 2198 2945 3213 160
Size: 747 268 888
Fragments arranged by size:
906 888 747 432 268 262 153 135
74 51 25
EcoNI CCTnn'n_nnAGG
Cuts at: 332 332
Size: 3941
EcoO109I rG'GnC_Cy
Cuts at: 1080 1080
Size: 3941
EcoRI G'AATT_C
Cuts at: 199 945 199
Size: 746 3195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3941
FspI TGC'GCA
Cuts at: 1722 1722
Size: 3941
HaeII r_GCGC'y
Cuts at: 1375 1623 2645 3624 1375
Size: 248 1022 979 1692
Fragments arranged by size:
1692 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1783 1813 1815 1845 1783
Size: 30 2 30 3879
Fragments arranged by size:
3879 30 30 2
HincII GTy'rAC
Cuts at: 219 732 980 219
Size: 513 248 3180
Fragments arranged by size:
3180 513 248
HindIII A'AGCT_T
Cuts at: 496 496
Size: 3941
KasI G'GCGC_C
Cuts at: 1371 1619 1371
Size: 248 3693
KpnI G_GTAC'C
Cuts at: 961 961
Size: 3941
MluI A'CGCG_T
Cuts at: 3374 3374
Size: 3941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2612 2796 2612
Size: 184 3757
MscI TGG'CCA
Cuts at: 1702 1702
Size: 3941
MslI CAynn'nnrTG
Cuts at: 369 1930 2057 369
Size: 1561 127 2253
Fragments arranged by size:
2253 1561 127
MspA1I CmG'CkG
Cuts at: 1052 1366 1726 2739 2984 1052
Size: 314 360 1013 245 2009
Fragments arranged by size:
2009 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2122 2122
Size: 3941
NarI GG'CG_CC
Cuts at: 1372 1620 1372
Size: 248 3693
NcoI C'CATG_G
Cuts at: 107 107
Size: 3941
NgoMIV G'CCGG_C
Cuts at: 2120 2120
Size: 3941
NheI G'CTAG_C
Cuts at: 421 576 421
Size: 155 3786
NsiI A_TGCA'T
Cuts at: 484 484
Size: 3941
NspI r_CATG'y
Cuts at: 994 1494 2401 994
Size: 500 907 2534
Fragments arranged by size:
2534 907 500
PciI A'CATG_T
Cuts at: 2397 2397
Size: 3941
PfoI T'CCnGG_A
Cuts at: 399 489 399
Size: 90 3851
PmeI GTTT'AAAC
Cuts at: 940 940
Size: 3941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3080 3112 3080
Size: 32 3909
PstI C_TGCA'G
Cuts at: 988 1673 988
Size: 685 3256
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3941
PvuII CAG'CTG
Cuts at: 1366 1726 1366
Size: 360 3581
RsrII CG'GwC_CG
Cuts at: 2136 2136
Size: 3941
SacI G_AGCT'C
Cuts at: 955 955
Size: 3941
SalI G'TCGA_C
Cuts at: 978 978
Size: 3941
SapI GCTCTTCn'nnn_
Cuts at: 1964 2174 1964
Size: 210 3731
SbfI CC_TGCA'GG
Cuts at: 988 988
Size: 3941
ScaI AGT'ACT
Cuts at: 3458 3458
Size: 3941
SexAI A'CCwGG_T
Cuts at: 819 819
Size: 3941
SfcI C'TryA_G
Cuts at: 33 275 984 1669 2662 2853 33
Size: 242 709 685 993 191 1121
Fragments arranged by size:
1121 993 709 685 242 191
SfoI GGC'GCC
Cuts at: 1373 1621 1373
Size: 248 3693
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
SmaI CCC'GGG
Cuts at: 963 963
Size: 3941
SmlI C'TyrA_G
Cuts at: 1096 2011 2503 2765 1096
Size: 915 492 262 2272
Fragments arranged by size:
2272 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3941
SphI G_CATG'C
Cuts at: 994 1494 994
Size: 500 3441
SspI AAT'ATT
Cuts at: 672 672
Size: 3941
StyI C'CwwG_G
Cuts at: 107 1075 1429 107
Size: 968 354 2619
Fragments arranged by size:
2619 968 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1524 1657 2116 2325 1524
Size: 133 459 209 3140
Fragments arranged by size:
3140 459 209 133
TatI w'GTAC_w
Cuts at: 594 3456 594
Size: 2862 1079
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 3909
Tth111I GACn'n_nGTC
Cuts at: 1738 1738
Size: 3941
XbaI T'CTAG_A
Cuts at: 972 972
Size: 3941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1436 1436
Size: 3941
XmaI C'CCGG_G
Cuts at: 961 961
Size: 3941
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BmgBI Bpu10I BsaXI BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI