Restriction map for
pF1KB6392
(Circular) (Six-base) MAPSORT of: pF1KB6392.seq Check: 8292 from: 1 to: 4022
pF1KB6392 4022 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1060 1060
Size: 4022
Acc65I G'GTAC_C
Cuts at: 390 1038 390
Size: 648 3374
AclI AA'CG_TT
Cuts at: 3862 3862
Size: 4022
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1847 2279 3026 3294 1847
Size: 432 747 268 2575
Fragments arranged by size:
2575 747 432 268
AflIII A'CryG_T
Cuts at: 1374 2478 3455 1374
Size: 1104 977 1941
Fragments arranged by size:
1941 1104 977
AgeI A'CCGG_T
Cuts at: 2410 2410
Size: 4022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 453 485 453
Size: 32 3990
AlwNI CAG_nnn'CTG
Cuts at: 222 1327 2894 222
Size: 1105 1567 1350
Fragments arranged by size:
1567 1350 1105
ApaLI G'TGCA_C
Cuts at: 2792 2792
Size: 4022
ApoI r'AATT_y
Cuts at: 427 559 1026 3375 3631 427
Size: 132 467 2349 256 818
Fragments arranged by size:
2349 818 467 256 132
AseI AT'TA_AT
Cuts at: 20 20
Size: 4022
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
AvaI C'yCGr_G
Cuts at: 507 1042 507
Size: 535 3487
BamHI G'GATC_C
Cuts at: 1047 1047
Size: 4022
BanI G'GyrC_C
Cuts at: 390 1038 1452 1700 1735 390
Size: 648 414 248 35 2677
Fragments arranged by size:
2677 648 414 248 35
BanII G_rGCy'C
Cuts at: 115 648 1036 2066 115
Size: 533 388 1030 2071
Fragments arranged by size:
2071 1030 533 388
BbeI G_GCGC'C
Cuts at: 1456 1704 1456
Size: 248 3774
BciVI GTATCCnnnnn_n'
Cuts at: 1920 2681 4022 1920
Size: 761 1341 1920
Fragments arranged by size:
1920 1341 761
BclI T'GATC_A
Cuts at: 1542 1542
Size: 4022
BglII A'GATC_T
Cuts at: 1537 3574 1537
Size: 2037 1985
BlpI GC'TnA_GC
Cuts at: 114 1134 114
Size: 1020 3002
Bme1580I G_kGCm'C
Cuts at: 499 688 1647 1740 2796 499
Size: 189 959 93 1056 1725
Fragments arranged by size:
1725 1056 959 189 93
BmrI ACTGGGnnnn_n'
Cuts at: 682 1234 1315 1650 3794 682
Size: 552 81 335 2144 910
Fragments arranged by size:
2144 910 552 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 226 760 185
Size: 41 534 3447
Fragments arranged by size:
3447 534 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 831 1198 2077 2569 2867 831
Size: 367 879 492 298 1986
Fragments arranged by size:
1986 879 492 367 298
BsaI GGTCTCn'nnnn_
Cuts at: 161 323 603 3726 161
Size: 162 280 3123 457
Fragments arranged by size:
3123 457 280 162
BsaAI yAC'GTr
Cuts at: 1375 1375
Size: 4022
BsaBI GATnn'nnATC
Cuts at: 6 1536 3573 6
Size: 1530 2037 455
Fragments arranged by size:
2037 1530 455
BsaHI Gr'CG_yC
Cuts at: 1453 1701 2403 3899 1453
Size: 248 702 1496 1576
Fragments arranged by size:
1576 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1211 1732 2410 2684 2831 3242 1211
Size: 521 678 274 147 411 1991
Fragments arranged by size:
1991 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1447 2205 2782 3278 1447
Size: 758 577 496 2191
Fragments arranged by size:
2191 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1564 1610 2818 104
Size: 1460 46 1208 1308
Fragments arranged by size:
1460 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 641 1036 1814 2004 2796 641
Size: 395 778 190 792 1867
Fragments arranged by size:
1867 792 778 395 190
BsmI GAATG_Cn'
Cuts at: 253 544 253
Size: 291 3731
Bsp1286I G_dGCh'C
Cuts at: 115 499 641 648 688 1036 1647 1740 1814
Size: 384 142 7 40 348 611 93 74
Cuts at: 1814 2004 2066 2796 115
Size: 190 62 730 1341
Fragments arranged by size:
1341 730 611 384 348 190 142 93
74 62 40 7
BspEI T'CCGG_A
Cuts at: 3242 3242
Size: 4022
BspHI T'CATG_A
Cuts at: 3198 3198
Size: 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1072 1286 1588 1969 1072
Size: 214 302 381 3125
Fragments arranged by size:
3125 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2314 2368 3852 4017 50
Size: 2264 54 1484 165 55
Fragments arranged by size:
2264 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 720 908 1934 720
Size: 188 1026 2808
Fragments arranged by size:
2808 1026 188
BsrFI r'CCGG_y
Cuts at: 2020 2201 2410 2020
Size: 181 209 3632
Fragments arranged by size:
3632 209 181
BssSI C'ACGA_G
Cuts at: 567 2293 2651 567
Size: 1726 358 1938
Fragments arranged by size:
1938 1726 358
BstAPI GCAn_nnn'nTGC
Cuts at: 203 263 272 746 1399 203
Size: 60 9 474 653 2826
Fragments arranged by size:
2826 653 474 60 9
BstBI TT'CG_AA
Cuts at: 2383 2383
Size: 4022
BstYI r'GATC_y
Cuts at: 1 1047 1514 1537 1872 2118 3130 3216 3228
Size: 1046 467 23 335 246 1012 86 12
Cuts at: 3228 3574 1
Size: 346 449
Fragments arranged by size:
1046 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 354 107
Size: 247 3775
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1972 2153 2316 1972
Size: 181 163 3678
Fragments arranged by size:
3678 181 163
BtsI GCAGTG_nn'
Cuts at: 688 1265 688
Size: 577 3445
DraI TTT'AAA
Cuts at: 1021 1021
Size: 4022
DrdI GACnn_nn'nnGTC
Cuts at: 1728 2586 1728
Size: 858 3164
EaeI y'GGCC_r
Cuts at: 840 1607 1781 2172 2199 3544 840
Size: 767 174 391 27 1345 1318
Fragments arranged by size:
1345 1318 767 391 174 27
EagI C'GGCC_G
Cuts at: 1607 1607
Size: 4022
EarI CTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2230 2540 2686 3832 2230
Size: 310 146 1146 2420
Fragments arranged by size:
2420 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1034 1034
Size: 4022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 226 760 1847 2279 3026 3294 185
Size: 41 534 1087 432 747 268 913
Fragments arranged by size:
1087 913 747 534 432 268 41
EcoO109I rG'GnC_Cy
Cuts at: 359 1161 359
Size: 802 3220
EcoRI G'AATT_C
Cuts at: 1026 1026
Size: 4022
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 4022
FspI TGC'GCA
Cuts at: 1803 1803
Size: 4022
HaeII r_GCGC'y
Cuts at: 1456 1704 2726 3705 1456
Size: 248 1022 979 1773
Fragments arranged by size:
1773 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1864 1894 1896 1926 1864
Size: 30 2 30 3960
Fragments arranged by size:
3960 30 30 2
HincII GTy'rAC
Cuts at: 1061 1061
Size: 4022
KasI G'GCGC_C
Cuts at: 1452 1700 1452
Size: 248 3774
KpnI G_GTAC'C
Cuts at: 394 1042 394
Size: 648 3374
MluI A'CGCG_T
Cuts at: 3455 3455
Size: 4022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2693 2877 2693
Size: 184 3838
MscI TGG'CCA
Cuts at: 842 1783 842
Size: 941 3081
MslI CAynn'nnrTG
Cuts at: 2011 2138 2011
Size: 127 3895
MspA1I CmG'CkG
Cuts at: 118 294 1133 1447 1807 2820 3065 118
Size: 176 839 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 839 360 314 245 176
NaeI GCC'GGC
Cuts at: 2203 2203
Size: 4022
NarI GG'CG_CC
Cuts at: 1453 1701 1453
Size: 248 3774
NcoI C'CATG_G
Cuts at: 107 107
Size: 4022
NgoMIV G'CCGG_C
Cuts at: 2201 2201
Size: 4022
NspI r_CATG'y
Cuts at: 291 592 1075 1575 2482 291
Size: 301 483 500 907 1831
Fragments arranged by size:
1831 907 500 483 301
PciI A'CATG_T
Cuts at: 2478 2478
Size: 4022
PflMI CCAn_nnn'nTGG
Cuts at: 783 783
Size: 4022
PfoI T'CCnGG_A
Cuts at: 162 242 162
Size: 80 3942
PmeI GTTT'AAAC
Cuts at: 1021 1021
Size: 4022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3990
PpuMI rG'GwC_Cy
Cuts at: 359 359
Size: 4022
PspXI vC'TCGA_Gb
Cuts at: 507 507
Size: 4022
PstI C_TGCA'G
Cuts at: 368 1069 1754 368
Size: 701 685 2636
Fragments arranged by size:
2636 701 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4022
PvuII CAG'CTG
Cuts at: 118 294 1447 1807 118
Size: 176 1153 360 2333
Fragments arranged by size:
2333 1153 360 176
RsrII CG'GwC_CG
Cuts at: 2217 2217
Size: 4022
SacI G_AGCT'C
Cuts at: 1036 1036
Size: 4022
SalI G'TCGA_C
Cuts at: 1059 1059
Size: 4022
SanDI GG'GwC_CC
Cuts at: 359 359
Size: 4022
SapI GCTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
SbfI CC_TGCA'GG
Cuts at: 368 1069 368
Size: 701 3321
ScaI AGT'ACT
Cuts at: 3539 3539
Size: 4022
SfcI C'TryA_G
Cuts at: 33 364 603 1065 1750 2743 2934 33
Size: 331 239 462 685 993 191 1121
Fragments arranged by size:
1121 993 685 462 331 239 191
SfoI GGC'GCC
Cuts at: 1454 1702 1454
Size: 248 3774
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
SmaI CCC'GGG
Cuts at: 1044 1044
Size: 4022
SmlI C'TyrA_G
Cuts at: 507 846 1177 2092 2584 2846 507
Size: 339 331 915 492 262 1683
Fragments arranged by size:
1683 915 492 339 331 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4022
SphI G_CATG'C
Cuts at: 592 1075 1575 592
Size: 483 500 3039
Fragments arranged by size:
3039 500 483
StyI C'CwwG_G
Cuts at: 107 914 1156 1510 107
Size: 807 242 354 2619
Fragments arranged by size:
2619 807 354 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1605 1738 2197 2406 1605
Size: 133 459 209 3221
Fragments arranged by size:
3221 459 209 133
TatI w'GTAC_w
Cuts at: 284 3537 284
Size: 3253 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 238 270 512 544 991 1023 3539 3571 238
Size: 32 242 32 447 32 2516 32 689
Fragments arranged by size:
2516 689 447 242 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1819 1819
Size: 4022
XbaI T'CTAG_A
Cuts at: 1053 1053
Size: 4022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1517 1517
Size: 4022
XhoI C'TCGA_G
Cuts at: 507 507
Size: 4022
XmaI C'CCGG_G
Cuts at: 1042 1042
Size: 4022
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI