Restriction map for
pF1KB6393
(Circular) (Six-base) MAPSORT of: pF1KB6393.seq Check: 2059 from: 1 to: 3941
pF1KB6393 3941 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 767 979 767
Size: 212 3729
Acc65I G'GTAC_C
Cuts at: 957 957
Size: 3941
AclI AA'CG_TT
Cuts at: 3781 3781
Size: 3941
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1766 2198 2945 3213 1766
Size: 432 747 268 2494
Fragments arranged by size:
2494 747 432 268
AflIII A'CryG_T
Cuts at: 1293 2397 3374 1293
Size: 1104 977 1860
Fragments arranged by size:
1860 1104 977
AgeI A'CCGG_T
Cuts at: 2329 2329
Size: 3941
AhdI GACnn_n'nnGTC
Cuts at: 207 207
Size: 3941
AlwNI CAG_nnn'CTG
Cuts at: 564 1246 2813 564
Size: 682 1567 1692
Fragments arranged by size:
1692 1567 682
ApaLI G'TGCA_C
Cuts at: 2711 2711
Size: 3941
ApoI r'AATT_y
Cuts at: 945 3294 3550 945
Size: 2349 256 1336
Fragments arranged by size:
2349 1336 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3941
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
AvaI C'yCGr_G
Cuts at: 543 739 961 543
Size: 196 222 3523
Fragments arranged by size:
3523 222 196
BamHI G'GATC_C
Cuts at: 966 966
Size: 3941
BanI G'GyrC_C
Cuts at: 957 1371 1619 1654 957
Size: 414 248 35 3244
Fragments arranged by size:
3244 414 248 35
BanII G_rGCy'C
Cuts at: 904 955 1985 904
Size: 51 1030 2860
Fragments arranged by size:
2860 1030 51
BbeI G_GCGC'C
Cuts at: 1375 1623 1375
Size: 248 3693
BbsI GAAGACnn'nnnn_
Cuts at: 703 703
Size: 3941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 169 203 169
Size: 34 3907
BciVI GTATCCnnnnn_n'
Cuts at: 1839 2600 3941 1839
Size: 761 1341 1839
Fragments arranged by size:
1839 1341 761
BclI T'GATC_A
Cuts at: 434 1461 434
Size: 1027 2914
BglII A'GATC_T
Cuts at: 1456 3493 1456
Size: 2037 1904
BlpI GC'TnA_GC
Cuts at: 1053 1053
Size: 3941
Bme1580I G_kGCm'C
Cuts at: 1566 1659 2715 1566
Size: 93 1056 2792
Fragments arranged by size:
2792 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1153 1234 1569 3713 1153
Size: 81 335 2144 1381
Fragments arranged by size:
2144 1381 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 3941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 654 729 586
Size: 68 75 3798
Fragments arranged by size:
3798 75 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1996 2488 2786 1117
Size: 879 492 298 2272
Fragments arranged by size:
2272 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 442 3645 442
Size: 3203 738
BsaAI yAC'GTr
Cuts at: 1294 1294
Size: 3941
BsaBI GATnn'nnATC
Cuts at: 6 1455 3492 6
Size: 1449 2037 455
Fragments arranged by size:
2037 1449 455
BsaHI Gr'CG_yC
Cuts at: 1372 1620 2322 3818 1372
Size: 248 702 1496 1495
Fragments arranged by size:
1496 1495 702 248
BsaWI w'CCGG_w
Cuts at: 1130 1651 2329 2603 2750 3161 1130
Size: 521 678 274 147 411 1910
Fragments arranged by size:
1910 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 826 856 826
Size: 30 3911
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 406 726 406
Size: 320 3621
BseYI C'CCAG_C
Cuts at: 654 1366 2124 2701 3197 654
Size: 712 758 577 496 1398
Fragments arranged by size:
1398 758 712 577 496
BsiEI CG_ry'CG
Cuts at: 104 1483 1529 2737 104
Size: 1379 46 1208 1308
Fragments arranged by size:
1379 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 620 904 955 1733 1923 2715 620
Size: 284 51 778 190 792 1846
Fragments arranged by size:
1846 792 778 284 190 51
BsmBI CGTCTCn'nnnn_
Cuts at: 215 215
Size: 3941
Bsp1286I G_dGCh'C
Cuts at: 620 904 955 1566 1659 1733 1923 1985 2715
Size: 284 51 611 93 74 190 62 730
Cuts at: 2715 620
Size: 1846
Fragments arranged by size:
1846 730 611 284 190 93 74 62
51
BspEI T'CCGG_A
Cuts at: 3161 3161
Size: 3941
BspHI T'CATG_A
Cuts at: 431 3117 431
Size: 2686 1255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 480 991 1205 1507 1888 480
Size: 511 214 302 381 2533
Fragments arranged by size:
2533 511 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2233 2287 3771 3936 50
Size: 2183 54 1484 165 55
Fragments arranged by size:
2183 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 803 1853 803
Size: 1050 2891
BsrFI r'CCGG_y
Cuts at: 1939 2120 2329 1939
Size: 181 209 3551
Fragments arranged by size:
3551 209 181
BssSI C'ACGA_G
Cuts at: 2212 2570 2212
Size: 358 3583
BstAPI GCAn_nnn'nTGC
Cuts at: 1318 1318
Size: 3941
BstBI TT'CG_AA
Cuts at: 2302 2302
Size: 3941
BstYI r'GATC_y
Cuts at: 1 966 1433 1456 1791 2037 3049 3135 3147
Size: 965 467 23 335 246 1012 86 12
Cuts at: 3147 3493 1
Size: 346 449
Fragments arranged by size:
1012 965 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 351 107
Size: 244 3697
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1891 2072 2235 1891
Size: 181 163 3597
Fragments arranged by size:
3597 181 163
BtsI GCAGTG_nn'
Cuts at: 665 1184 665
Size: 519 3422
DraI TTT'AAA
Cuts at: 940 940
Size: 3941
DrdI GACnn_nn'nnGTC
Cuts at: 418 1647 2505 418
Size: 1229 858 1854
Fragments arranged by size:
1854 1229 858
EaeI y'GGCC_r
Cuts at: 338 1526 1700 2091 2118 3463 338
Size: 1188 174 391 27 1345 816
Fragments arranged by size:
1345 1188 816 391 174 27
EagI C'GGCC_G
Cuts at: 1526 1526
Size: 3941
EarI CTCTTCn'nnn_
Cuts at: 1964 2174 1964
Size: 210 3731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 226 2149 2459 2605 3751 226
Size: 1923 310 146 1146 416
Fragments arranged by size:
1923 1146 416 310 146
EcoICRI GAG'CTC
Cuts at: 902 953 902
Size: 51 3890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 654 729 1766 2198 2945 3213 586
Size: 68 75 1037 432 747 268 1314
Fragments arranged by size:
1314 1037 747 432 268 75 68
EcoO109I rG'GnC_Cy
Cuts at: 149 369 570 665 735 1080 149
Size: 220 201 95 70 345 3010
Fragments arranged by size:
3010 345 220 201 95 70
EcoRI G'AATT_C
Cuts at: 945 945
Size: 3941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3941
FspI TGC'GCA
Cuts at: 1722 1722
Size: 3941
HaeII r_GCGC'y
Cuts at: 1375 1623 2645 3624 1375
Size: 248 1022 979 1692
Fragments arranged by size:
1692 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 826 858 1783 1813 1815 1845 193
Size: 32 601 32 925 30 2 30 2289
Fragments arranged by size:
2289 925 601 32 32 30 30 2
HincII GTy'rAC
Cuts at: 980 980
Size: 3941
KasI G'GCGC_C
Cuts at: 1371 1619 1371
Size: 248 3693
KpnI G_GTAC'C
Cuts at: 961 961
Size: 3941
MluI A'CGCG_T
Cuts at: 3374 3374
Size: 3941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2612 2796 2612
Size: 184 3757
MscI TGG'CCA
Cuts at: 340 1702 340
Size: 1362 2579
MslI CAynn'nnrTG
Cuts at: 1930 2057 1930
Size: 127 3814
MspA1I CmG'CkG
Cuts at: 405 1052 1366 1726 2739 2984 405
Size: 647 314 360 1013 245 1362
Fragments arranged by size:
1362 1013 647 360 314 245
NaeI GCC'GGC
Cuts at: 2122 2122
Size: 3941
NarI GG'CG_CC
Cuts at: 1372 1620 1372
Size: 248 3693
NcoI C'CATG_G
Cuts at: 107 351 107
Size: 244 3697
NgoMIV G'CCGG_C
Cuts at: 2120 2120
Size: 3941
NspI r_CATG'y
Cuts at: 994 1494 2401 994
Size: 500 907 2534
Fragments arranged by size:
2534 907 500
PciI A'CATG_T
Cuts at: 2397 2397
Size: 3941
PflMI CCAn_nnn'nTGG
Cuts at: 527 564 527
Size: 37 3904
PmeI GTTT'AAAC
Cuts at: 940 940
Size: 3941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3080 3112 3080
Size: 32 3909
PpuMI rG'GwC_Cy
Cuts at: 570 735 570
Size: 165 3776
PstI C_TGCA'G
Cuts at: 404 731 988 1673 404
Size: 327 257 685 2672
Fragments arranged by size:
2672 685 327 257
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3941
PvuII CAG'CTG
Cuts at: 405 1366 1726 405
Size: 961 360 2620
Fragments arranged by size:
2620 961 360
RsrII CG'GwC_CG
Cuts at: 2136 2136
Size: 3941
SacI G_AGCT'C
Cuts at: 904 955 904
Size: 51 3890
SalI G'TCGA_C
Cuts at: 978 978
Size: 3941
SapI GCTCTTCn'nnn_
Cuts at: 1964 2174 1964
Size: 210 3731
SbfI CC_TGCA'GG
Cuts at: 988 988
Size: 3941
ScaI AGT'ACT
Cuts at: 3458 3458
Size: 3941
SexAI A'CCwGG_T
Cuts at: 572 572
Size: 3941
SfcI C'TryA_G
Cuts at: 33 400 727 984 1669 2662 2853 33
Size: 367 327 257 685 993 191 1121
Fragments arranged by size:
1121 993 685 367 327 257 191
SfoI GGC'GCC
Cuts at: 1373 1621 1373
Size: 248 3693
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
SmaI CCC'GGG
Cuts at: 963 963
Size: 3941
SmlI C'TyrA_G
Cuts at: 1096 2011 2503 2765 1096
Size: 915 492 262 2272
Fragments arranged by size:
2272 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3941
SphI G_CATG'C
Cuts at: 994 1494 994
Size: 500 3441
StyI C'CwwG_G
Cuts at: 107 351 1075 1429 107
Size: 244 724 354 2619
Fragments arranged by size:
2619 724 354 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1524 1657 2116 2325 1524
Size: 133 459 209 3140
Fragments arranged by size:
3140 459 209 133
TatI w'GTAC_w
Cuts at: 3456 3456
Size: 3941
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 320 352 3458 3490 320
Size: 32 3106 32 771
Fragments arranged by size:
3106 771 32 32
Tth111I GACn'n_nGTC
Cuts at: 1738 1738
Size: 3941
XbaI T'CTAG_A
Cuts at: 972 972
Size: 3941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1436 1436
Size: 3941
XmaI C'CCGG_G
Cuts at: 961 961
Size: 3941
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI