Restriction map for
pF1KB6395
(Circular) (Six-base) MAPSORT of: pF1KB6395.seq Check: 9662 from: 1 to: 3941
pF1KB6395 3941 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 979 979
Size: 3941
Acc65I G'GTAC_C
Cuts at: 957 957
Size: 3941
AclI AA'CG_TT
Cuts at: 299 3781 299
Size: 3482 459
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1766 2198 2945 3213 791
Size: 975 432 747 268 1519
Fragments arranged by size:
1519 975 747 432 268
AflIII A'CryG_T
Cuts at: 1293 2397 3374 1293
Size: 1104 977 1860
Fragments arranged by size:
1860 1104 977
AgeI A'CCGG_T
Cuts at: 2329 2329
Size: 3941
AlwNI CAG_nnn'CTG
Cuts at: 276 1246 2813 276
Size: 970 1567 1404
Fragments arranged by size:
1567 1404 970
ApaLI G'TGCA_C
Cuts at: 2711 2711
Size: 3941
ApoI r'AATT_y
Cuts at: 262 945 3294 3550 262
Size: 683 2349 256 653
Fragments arranged by size:
2349 683 653 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3941
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
AvaI C'yCGr_G
Cuts at: 137 242 377 961 137
Size: 105 135 584 3117
Fragments arranged by size:
3117 584 135 105
BamHI G'GATC_C
Cuts at: 966 966
Size: 3941
BanI G'GyrC_C
Cuts at: 322 451 957 1371 1619 1654 322
Size: 129 506 414 248 35 2609
Fragments arranged by size:
2609 506 414 248 129 35
BanII G_rGCy'C
Cuts at: 135 233 955 1985 135
Size: 98 722 1030 2091
Fragments arranged by size:
2091 1030 722 98
BbeI G_GCGC'C
Cuts at: 1375 1623 1375
Size: 248 3693
BbvCI CC'TCA_GC
Cuts at: 384 384
Size: 3941
BciVI GTATCCnnnnn_n'
Cuts at: 1839 2600 3941 1839
Size: 761 1341 1839
Fragments arranged by size:
1839 1341 761
BclI T'GATC_A
Cuts at: 1461 1461
Size: 3941
BglII A'GATC_T
Cuts at: 1456 3493 1456
Size: 2037 1904
BlpI GC'TnA_GC
Cuts at: 1053 1053
Size: 3941
Bme1580I G_kGCm'C
Cuts at: 1566 1659 2715 1566
Size: 93 1056 2792
Fragments arranged by size:
2792 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 495 1153 1234 1569 3713 495
Size: 658 81 335 2144 723
Fragments arranged by size:
2144 723 658 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 3941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 854 209
Size: 645 3296
Bpu10I CC'TnA_GC
Cuts at: 384 384
Size: 3941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1996 2488 2786 1117
Size: 879 492 298 2272
Fragments arranged by size:
2272 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3645 3645
Size: 3941
BsaAI yAC'GTr
Cuts at: 663 1294 663
Size: 631 3310
BsaBI GATnn'nnATC
Cuts at: 6 1455 3492 6
Size: 1449 2037 455
Fragments arranged by size:
2037 1449 455
BsaHI Gr'CG_yC
Cuts at: 183 1372 1620 2322 3818 183
Size: 1189 248 702 1496 306
Fragments arranged by size:
1496 1189 702 306 248
BsaWI w'CCGG_w
Cuts at: 1130 1651 2329 2603 2750 3161 1130
Size: 521 678 274 147 411 1910
Fragments arranged by size:
1910 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 827 857 827
Size: 30 3911
BseYI C'CCAG_C
Cuts at: 388 632 1366 2124 2701 3197 388
Size: 244 734 758 577 496 1132
Fragments arranged by size:
1132 758 734 577 496 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 895 488
Size: 407 3534
BsiEI CG_ry'CG
Cuts at: 104 151 1483 1529 2737 104
Size: 47 1332 46 1208 1308
Fragments arranged by size:
1332 1308 1208 47 46
BsiHKAI G_wGCw'C
Cuts at: 233 955 1733 1923 2715 233
Size: 722 778 190 792 1459
Fragments arranged by size:
1459 792 778 722 190
BsmI GAATG_Cn'
Cuts at: 254 514 254
Size: 260 3681
Bsp1286I G_dGCh'C
Cuts at: 135 233 955 1566 1659 1733 1923 1985 2715
Size: 98 722 611 93 74 190 62 730
Cuts at: 2715 135
Size: 1361
Fragments arranged by size:
1361 730 722 611 190 98 93 74
62
BspEI T'CCGG_A
Cuts at: 3161 3161
Size: 3941
BspHI T'CATG_A
Cuts at: 3117 3117
Size: 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 452 914 991 1205 1507 1888 452
Size: 462 77 214 302 381 2505
Fragments arranged by size:
2505 462 381 302 214 77
BsrBI CCG'CTC
Cuts at: 50 2233 2287 3771 3936 50
Size: 2183 54 1484 165 55
Fragments arranged by size:
2183 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1853 1853
Size: 3941
BsrFI r'CCGG_y
Cuts at: 1939 2120 2329 1939
Size: 181 209 3551
Fragments arranged by size:
3551 209 181
BssSI C'ACGA_G
Cuts at: 2212 2570 2212
Size: 358 3583
BstAPI GCAn_nnn'nTGC
Cuts at: 1318 1318
Size: 3941
BstBI TT'CG_AA
Cuts at: 2302 2302
Size: 3941
BstYI r'GATC_y
Cuts at: 1 966 1433 1456 1791 2037 3049 3135 3147
Size: 965 467 23 335 246 1012 86 12
Cuts at: 3147 3493 1
Size: 346 449
Fragments arranged by size:
1012 965 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 318 704 107
Size: 211 386 3344
Fragments arranged by size:
3344 386 211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1891 2072 2235 1891
Size: 181 163 3597
Fragments arranged by size:
3597 181 163
BtsI GCAGTG_nn'
Cuts at: 374 1184 374
Size: 810 3131
DraI TTT'AAA
Cuts at: 940 940
Size: 3941
DrdI GACnn_nn'nnGTC
Cuts at: 1647 2505 1647
Size: 858 3083
EaeI y'GGCC_r
Cuts at: 148 500 789 1526 1700 2091 2118 3463 148
Size: 352 289 737 174 391 27 1345 626
Fragments arranged by size:
1345 737 626 391 352 289 174 27
EagI C'GGCC_G
Cuts at: 148 1526 148
Size: 1378 2563
EarI CTCTTCn'nnn_
Cuts at: 767 1964 2174 767
Size: 1197 210 2534
Fragments arranged by size:
2534 1197 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2149 2459 2605 3751 2149
Size: 310 146 1146 2339
Fragments arranged by size:
2339 1146 310 146
EcoICRI GAG'CTC
Cuts at: 231 953 231
Size: 722 3219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 791 854 1766 2198 2945 3213 209
Size: 582 63 912 432 747 268 937
Fragments arranged by size:
937 912 747 582 432 268 63
EcoO109I rG'GnC_Cy
Cuts at: 392 646 1080 392
Size: 254 434 3253
Fragments arranged by size:
3253 434 254
EcoRI G'AATT_C
Cuts at: 262 945 262
Size: 683 3258
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3941
FspI TGC'GCA
Cuts at: 1722 1722
Size: 3941
HaeII r_GCGC'y
Cuts at: 115 495 1375 1623 2645 3624 115
Size: 380 880 248 1022 979 432
Fragments arranged by size:
1022 979 880 432 380 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 827 859 1783 1813 1815 1845 827
Size: 32 924 30 2 30 2923
Fragments arranged by size:
2923 924 32 30 30 2
HincII GTy'rAC
Cuts at: 980 980
Size: 3941
KasI G'GCGC_C
Cuts at: 1371 1619 1371
Size: 248 3693
KpnI G_GTAC'C
Cuts at: 961 961
Size: 3941
MluI A'CGCG_T
Cuts at: 3374 3374
Size: 3941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 599 2612 2796 599
Size: 2013 184 1744
Fragments arranged by size:
2013 1744 184
MscI TGG'CCA
Cuts at: 502 1702 502
Size: 1200 2741
MslI CAynn'nnrTG
Cuts at: 1930 2057 1930
Size: 127 3814
MspA1I CmG'CkG
Cuts at: 147 388 1052 1366 1726 2739 2984 147
Size: 241 664 314 360 1013 245 1104
Fragments arranged by size:
1104 1013 664 360 314 245 241
NaeI GCC'GGC
Cuts at: 2122 2122
Size: 3941
NarI GG'CG_CC
Cuts at: 1372 1620 1372
Size: 248 3693
NcoI C'CATG_G
Cuts at: 107 318 107
Size: 211 3730
NgoMIV G'CCGG_C
Cuts at: 2120 2120
Size: 3941
NspI r_CATG'y
Cuts at: 994 1494 2401 994
Size: 500 907 2534
Fragments arranged by size:
2534 907 500
PasI CC'CwG_GG
Cuts at: 276 276
Size: 3941
PciI A'CATG_T
Cuts at: 2397 2397
Size: 3941
PflMI CCAn_nnn'nTGG
Cuts at: 276 276
Size: 3941
PfoI T'CCnGG_A
Cuts at: 648 648
Size: 3941
PmeI GTTT'AAAC
Cuts at: 940 940
Size: 3941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3080 3112 3080
Size: 32 3909
PpuMI rG'GwC_Cy
Cuts at: 392 646 392
Size: 254 3687
PstI C_TGCA'G
Cuts at: 988 1673 988
Size: 685 3256
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3941
PvuII CAG'CTG
Cuts at: 388 1366 1726 388
Size: 978 360 2603
Fragments arranged by size:
2603 978 360
RsrII CG'GwC_CG
Cuts at: 2136 2136
Size: 3941
SacI G_AGCT'C
Cuts at: 233 955 233
Size: 722 3219
SalI G'TCGA_C
Cuts at: 978 978
Size: 3941
SapI GCTCTTCn'nnn_
Cuts at: 1964 2174 1964
Size: 210 3731
SbfI CC_TGCA'GG
Cuts at: 988 988
Size: 3941
ScaI AGT'ACT
Cuts at: 484 3458 484
Size: 2974 967
SfcI C'TryA_G
Cuts at: 33 204 984 1669 2662 2853 33
Size: 171 780 685 993 191 1121
Fragments arranged by size:
1121 993 780 685 191 171
SfoI GGC'GCC
Cuts at: 1373 1621 1373
Size: 248 3693
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
SmaI CCC'GGG
Cuts at: 244 963 244
Size: 719 3222
SmlI C'TyrA_G
Cuts at: 1096 2011 2503 2765 1096
Size: 915 492 262 2272
Fragments arranged by size:
2272 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3941
SphI G_CATG'C
Cuts at: 994 1494 994
Size: 500 3441
StuI AGG'CCT
Cuts at: 778 778
Size: 3941
StyI C'CwwG_G
Cuts at: 107 318 503 786 1075 1429 107
Size: 211 185 283 289 354 2619
Fragments arranged by size:
2619 354 289 283 211 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 268 864 1524 1657 2116 2325 268
Size: 596 660 133 459 209 1884
Fragments arranged by size:
1884 660 596 459 209 133
TatI w'GTAC_w
Cuts at: 482 518 889 3456 482
Size: 36 371 2567 967
Fragments arranged by size:
2567 967 371 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 3909
Tth111I GACn'n_nGTC
Cuts at: 1738 1738
Size: 3941
XbaI T'CTAG_A
Cuts at: 972 972
Size: 3941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 277 1436 277
Size: 1159 2782
XmaI C'CCGG_G
Cuts at: 242 961 242
Size: 719 3222
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BseRI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI