Restriction map for
pF1KB6396
(Circular) (Six-base) MAPSORT of: pF1KB6396.seq Check: 1295 from: 1 to: 4025
pF1KB6396 4025 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 857 857
Size: 4025
AccI GT'mk_AC
Cuts at: 1063 1063
Size: 4025
Acc65I G'GTAC_C
Cuts at: 1041 1041
Size: 4025
AclI AA'CG_TT
Cuts at: 3865 3865
Size: 4025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 1850 2282 3029 3297 626
Size: 1224 432 747 268 1354
Fragments arranged by size:
1354 1224 747 432 268
AflIII A'CryG_T
Cuts at: 1377 2481 3458 1377
Size: 1104 977 1944
Fragments arranged by size:
1944 1104 977
AgeI A'CCGG_T
Cuts at: 2413 2413
Size: 4025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 301 875 301
Size: 574 3451
AlwNI CAG_nnn'CTG
Cuts at: 1330 2897 1330
Size: 1567 2458
ApaLI G'TGCA_C
Cuts at: 905 2795 905
Size: 1890 2135
ApoI r'AATT_y
Cuts at: 216 648 731 1029 3378 3634 216
Size: 432 83 298 2349 256 607
Fragments arranged by size:
2349 607 432 298 256 83
AseI AT'TA_AT
Cuts at: 20 20
Size: 4025
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
AvaI C'yCGr_G
Cuts at: 1045 1045
Size: 4025
BamHI G'GATC_C
Cuts at: 1050 1050
Size: 4025
BanI G'GyrC_C
Cuts at: 413 1041 1455 1703 1738 413
Size: 628 414 248 35 2700
Fragments arranged by size:
2700 628 414 248 35
BanII G_rGCy'C
Cuts at: 436 1039 2069 436
Size: 603 1030 2392
Fragments arranged by size:
2392 1030 603
BbeI G_GCGC'C
Cuts at: 1459 1707 1459
Size: 248 3777
BbsI GAAGACnn'nnnn_
Cuts at: 959 959
Size: 4025
BciVI GTATCCnnnnn_n'
Cuts at: 1923 2684 4025 1923
Size: 761 1341 1923
Fragments arranged by size:
1923 1341 761
BclI T'GATC_A
Cuts at: 1545 1545
Size: 4025
BfrBI ATG'CAT
Cuts at: 457 457
Size: 4025
BglI GCCn_nnn'nGGC
Cuts at: 706 724 706
Size: 18 4007
BglII A'GATC_T
Cuts at: 1540 3577 1540
Size: 2037 1988
BlpI GC'TnA_GC
Cuts at: 430 1137 430
Size: 707 3318
Bme1580I G_kGCm'C
Cuts at: 909 970 1650 1743 2799 909
Size: 61 680 93 1056 2135
Fragments arranged by size:
2135 1056 680 93 61
BmrI ACTGGGnnnn_n'
Cuts at: 1237 1318 1653 3797 1237
Size: 81 335 2144 1465
Fragments arranged by size:
2144 1465 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 183 2392 183
Size: 2209 1816
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 1201 2080 2572 2870 685
Size: 516 879 492 298 1840
Fragments arranged by size:
1840 879 516 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3729 3729
Size: 4025
BsaAI yAC'GTr
Cuts at: 1378 1378
Size: 4025
BsaBI GATnn'nnATC
Cuts at: 6 346 1539 3576 6
Size: 340 1193 2037 455
Fragments arranged by size:
2037 1193 455 340
BsaHI Gr'CG_yC
Cuts at: 1456 1704 2406 3902 1456
Size: 248 702 1496 1579
Fragments arranged by size:
1579 1496 702 248
BsaWI w'CCGG_w
Cuts at: 530 1214 1735 2413 2687 2834 3245 530
Size: 684 521 678 274 147 411 1310
Fragments arranged by size:
1310 684 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 585 615 888 918 585
Size: 30 273 30 3692
Fragments arranged by size:
3692 273 30 30
BseYI C'CCAG_C
Cuts at: 1450 2208 2785 3281 1450
Size: 758 577 496 2194
Fragments arranged by size:
2194 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1567 1613 2821 104
Size: 1463 46 1208 1308
Fragments arranged by size:
1463 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 290 314 436 909 1039 1817 2007 2799 290
Size: 24 122 473 130 778 190 792 1516
Fragments arranged by size:
1516 792 778 473 190 130 122 24
Bsp1286I G_dGCh'C
Cuts at: 290 314 436 909 970 1039 1650 1743 1817
Size: 24 122 473 61 69 611 93 74
Cuts at: 1817 2007 2069 2799 290
Size: 190 62 730 1516
Fragments arranged by size:
1516 730 611 473 190 122 93 74
69 62 61 24
BspEI T'CCGG_A
Cuts at: 530 3245 530
Size: 2715 1310
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 857 1075 1289 1591 1972 857
Size: 218 214 302 381 2910
Fragments arranged by size:
2910 381 302 218 214
BsrBI CCG'CTC
Cuts at: 50 2317 2371 3855 4020 50
Size: 2267 54 1484 165 55
Fragments arranged by size:
2267 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 538 1937 538
Size: 1399 2626
BsrFI r'CCGG_y
Cuts at: 2023 2204 2413 2023
Size: 181 209 3635
Fragments arranged by size:
3635 209 181
BssSI C'ACGA_G
Cuts at: 2296 2654 2296
Size: 358 3667
BstAPI GCAn_nnn'nTGC
Cuts at: 1402 1402
Size: 4025
BstBI TT'CG_AA
Cuts at: 2386 2386
Size: 4025
BstYI r'GATC_y
Cuts at: 1 1050 1517 1540 1875 2121 3133 3219 3231
Size: 1049 467 23 335 246 1012 86 12
Cuts at: 3231 3577 1
Size: 346 449
Fragments arranged by size:
1049 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 807 807
Size: 4025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1975 2156 2319 1975
Size: 181 163 3681
Fragments arranged by size:
3681 181 163
BtsI GCAGTG_nn'
Cuts at: 653 767 1268 653
Size: 114 501 3410
Fragments arranged by size:
3410 501 114
DraI TTT'AAA
Cuts at: 1024 1024
Size: 4025
DrdI GACnn_nn'nnGTC
Cuts at: 1731 2589 1731
Size: 858 3167
EaeI y'GGCC_r
Cuts at: 1610 1784 2175 2202 3547 1610
Size: 174 391 27 1345 2088
Fragments arranged by size:
2088 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1610 1610
Size: 4025
EarI CTCTTCn'nnn_
Cuts at: 182 620 916 2048 2258 182
Size: 438 296 1132 210 1949
Fragments arranged by size:
1949 1132 438 296 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2233 2543 2689 3835 2233
Size: 310 146 1146 2423
Fragments arranged by size:
2423 1146 310 146
EcoICRI GAG'CTC
Cuts at: 434 1037 434
Size: 603 3422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 1850 2282 3029 3297 626
Size: 1224 432 747 268 1354
Fragments arranged by size:
1354 1224 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1164 1164
Size: 4025
EcoRI G'AATT_C
Cuts at: 1029 1029
Size: 4025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 631 2044 631
Size: 1413 2612
FspI TGC'GCA
Cuts at: 1806 1806
Size: 4025
HaeII r_GCGC'y
Cuts at: 468 1459 1707 2729 3708 468
Size: 991 248 1022 979 785
Fragments arranged by size:
1022 991 979 785 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 585 617 1867 1897 1899 1929 585
Size: 32 1250 30 2 30 2681
Fragments arranged by size:
2681 1250 32 30 30 2
HincII GTy'rAC
Cuts at: 1064 1064
Size: 4025
KasI G'GCGC_C
Cuts at: 1455 1703 1455
Size: 248 3777
KpnI G_GTAC'C
Cuts at: 1045 1045
Size: 4025
MfeI C'AATT_G
Cuts at: 221 421 221
Size: 200 3825
MluI A'CGCG_T
Cuts at: 3458 3458
Size: 4025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2696 2880 2696
Size: 184 3841
MscI TGG'CCA
Cuts at: 1786 1786
Size: 4025
MslI CAynn'nnrTG
Cuts at: 2014 2141 2014
Size: 127 3898
MspA1I CmG'CkG
Cuts at: 1136 1450 1810 2823 3068 1136
Size: 314 360 1013 245 2093
Fragments arranged by size:
2093 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2206 2206
Size: 4025
NarI GG'CG_CC
Cuts at: 1456 1704 1456
Size: 248 3777
NcoI C'CATG_G
Cuts at: 807 807
Size: 4025
NgoMIV G'CCGG_C
Cuts at: 2204 2204
Size: 4025
NsiI A_TGCA'T
Cuts at: 459 459
Size: 4025
NspI r_CATG'y
Cuts at: 1078 1578 2485 1078
Size: 500 907 2618
Fragments arranged by size:
2618 907 500
PciI A'CATG_T
Cuts at: 2481 2481
Size: 4025
PflMI CCAn_nnn'nTGG
Cuts at: 450 450
Size: 4025
PmeI GTTT'AAAC
Cuts at: 1024 1024
Size: 4025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3993
PstI C_TGCA'G
Cuts at: 828 854 1072 1757 828
Size: 26 218 685 3096
Fragments arranged by size:
3096 685 218 26
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4025
PvuII CAG'CTG
Cuts at: 1450 1810 1450
Size: 360 3665
RsrII CG'GwC_CG
Cuts at: 2220 2220
Size: 4025
SacI G_AGCT'C
Cuts at: 436 1039 436
Size: 603 3422
SalI G'TCGA_C
Cuts at: 1062 1062
Size: 4025
SapI GCTCTTCn'nnn_
Cuts at: 620 2048 2258 620
Size: 1428 210 2387
Fragments arranged by size:
2387 1428 210
SbfI CC_TGCA'GG
Cuts at: 1072 1072
Size: 4025
ScaI AGT'ACT
Cuts at: 3542 3542
Size: 4025
SfcI C'TryA_G
Cuts at: 33 824 850 1068 1753 2746 2937 33
Size: 791 26 218 685 993 191 1121
Fragments arranged by size:
1121 993 791 685 218 191 26
SfoI GGC'GCC
Cuts at: 1457 1705 1457
Size: 248 3777
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
SmaI CCC'GGG
Cuts at: 1047 1047
Size: 4025
SmlI C'TyrA_G
Cuts at: 664 1180 2095 2587 2849 664
Size: 516 915 492 262 1840
Fragments arranged by size:
1840 915 516 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4025
SphI G_CATG'C
Cuts at: 1078 1578 1078
Size: 500 3525
StuI AGG'CCT
Cuts at: 781 781
Size: 4025
StyI C'CwwG_G
Cuts at: 807 1159 1513 807
Size: 352 354 3319
Fragments arranged by size:
3319 354 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1608 1741 2200 2409 1608
Size: 133 459 209 3224
Fragments arranged by size:
3224 459 209 133
TatI w'GTAC_w
Cuts at: 3540 3540
Size: 4025
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 888 920 3542 3574 888
Size: 32 2622 32 1339
Fragments arranged by size:
2622 1339 32 32
Tth111I GACn'n_nGTC
Cuts at: 1822 1822
Size: 4025
XbaI T'CTAG_A
Cuts at: 1056 1056
Size: 4025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1520 1520
Size: 4025
XmaI C'CCGG_G
Cuts at: 1045 1045
Size: 4025
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BmgBI BmtI BpmI Bpu10I
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI