Restriction map for
pF1KB6403
(Circular) (Six-base) MAPSORT of: pF1KB6403.seq Check: 7776 from: 1 to: 4025
pF1KB6403 4025 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1063 1063
Size: 4025
Acc65I G'GTAC_C
Cuts at: 1041 1041
Size: 4025
AclI AA'CG_TT
Cuts at: 3865 3865
Size: 4025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 1028 1850 2282 3029 3297 365
Size: 663 822 432 747 268 1093
Fragments arranged by size:
1093 822 747 663 432 268
AfeI AGC'GCT
Cuts at: 1005 1005
Size: 4025
AflIII A'CryG_T
Cuts at: 1377 2481 3458 1377
Size: 1104 977 1944
Fragments arranged by size:
1944 1104 977
AgeI A'CCGG_T
Cuts at: 2413 2413
Size: 4025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 234 234
Size: 4025
AlwNI CAG_nnn'CTG
Cuts at: 343 733 1330 2897 343
Size: 390 597 1567 1471
Fragments arranged by size:
1567 1471 597 390
ApaLI G'TGCA_C
Cuts at: 2795 2795
Size: 4025
ApoI r'AATT_y
Cuts at: 610 1029 3378 3634 610
Size: 419 2349 256 1001
Fragments arranged by size:
2349 1001 419 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4025
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
AvaI C'yCGr_G
Cuts at: 1045 1045
Size: 4025
BamHI G'GATC_C
Cuts at: 450 1050 450
Size: 600 3425
BanI G'GyrC_C
Cuts at: 1041 1455 1703 1738 1041
Size: 414 248 35 3328
Fragments arranged by size:
3328 414 248 35
BanII G_rGCy'C
Cuts at: 1039 2069 1039
Size: 1030 2995
BbeI G_GCGC'C
Cuts at: 1459 1707 1459
Size: 248 3777
BciVI GTATCCnnnnn_n'
Cuts at: 726 1923 2684 4025 726
Size: 1197 761 1341 726
Fragments arranged by size:
1341 1197 761 726
BclI T'GATC_A
Cuts at: 1545 1545
Size: 4025
BglII A'GATC_T
Cuts at: 648 1540 3577 648
Size: 892 2037 1096
Fragments arranged by size:
2037 1096 892
BlpI GC'TnA_GC
Cuts at: 926 1137 926
Size: 211 3814
Bme1580I G_kGCm'C
Cuts at: 1650 1743 2799 1650
Size: 93 1056 2876
Fragments arranged by size:
2876 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1237 1318 1653 3797 1237
Size: 81 335 2144 1465
Fragments arranged by size:
2144 1465 335 81
BmtI G_CTAG'C
Cuts at: 758 758
Size: 4025
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2392 2392
Size: 4025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 890
Size: 4025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 1201 2080 2572 2870 797
Size: 404 879 492 298 1952
Fragments arranged by size:
1952 879 492 404 298
BsaI GGTCTCn'nnnn_
Cuts at: 3729 3729
Size: 4025
BsaAI yAC'GTr
Cuts at: 1378 1378
Size: 4025
BsaBI GATnn'nnATC
Cuts at: 6 1539 3576 6
Size: 1533 2037 455
Fragments arranged by size:
2037 1533 455
BsaHI Gr'CG_yC
Cuts at: 1456 1704 2406 3902 1456
Size: 248 702 1496 1579
Fragments arranged by size:
1579 1496 702 248
BsaWI w'CCGG_w
Cuts at: 980 1214 1735 2413 2687 2834 3245 980
Size: 234 521 678 274 147 411 1760
Fragments arranged by size:
1760 678 521 411 274 234 147
BseYI C'CCAG_C
Cuts at: 1450 2208 2785 3281 1450
Size: 758 577 496 2194
Fragments arranged by size:
2194 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1567 1613 2821 104
Size: 1463 46 1208 1308
Fragments arranged by size:
1463 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1039 1817 2007 2799 1039
Size: 778 190 792 2265
Fragments arranged by size:
2265 792 778 190
BsmI GAATG_Cn'
Cuts at: 1014 1014
Size: 4025
Bsp1286I G_dGCh'C
Cuts at: 1039 1650 1743 1817 2007 2069 2799 1039
Size: 611 93 74 190 62 730 2265
Fragments arranged by size:
2265 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 980 3245 980
Size: 2265 1760
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 870 1075 1289 1591 1972 870
Size: 205 214 302 381 2923
Fragments arranged by size:
2923 381 302 214 205
BsrBI CCG'CTC
Cuts at: 50 2317 2371 3855 4020 50
Size: 2267 54 1484 165 55
Fragments arranged by size:
2267 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1937 1937
Size: 4025
BsrFI r'CCGG_y
Cuts at: 2023 2204 2413 2023
Size: 181 209 3635
Fragments arranged by size:
3635 209 181
BssSI C'ACGA_G
Cuts at: 2296 2654 2296
Size: 358 3667
BstAPI GCAn_nnn'nTGC
Cuts at: 1402 1402
Size: 4025
BstBI TT'CG_AA
Cuts at: 2386 2386
Size: 4025
BstYI r'GATC_y
Cuts at: 1 450 648 1050 1517 1540 1875 2121 3133
Size: 449 198 402 467 23 335 246 1012
Cuts at: 3133 3219 3231 3577 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 449 402 346 335 246
198 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1975 2156 2319 1975
Size: 181 163 3681
Fragments arranged by size:
3681 181 163
BtsI GCAGTG_nn'
Cuts at: 425 596 1268 425
Size: 171 672 3182
Fragments arranged by size:
3182 672 171
DraI TTT'AAA
Cuts at: 1024 1024
Size: 4025
DrdI GACnn_nn'nnGTC
Cuts at: 1731 2589 1731
Size: 858 3167
EaeI y'GGCC_r
Cuts at: 1610 1784 2175 2202 3547 1610
Size: 174 391 27 1345 2088
Fragments arranged by size:
2088 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1610 1610
Size: 4025
EarI CTCTTCn'nnn_
Cuts at: 1004 2048 2258 1004
Size: 1044 210 2771
Fragments arranged by size:
2771 1044 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2233 2543 2689 3835 2233
Size: 310 146 1146 2423
Fragments arranged by size:
2423 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1037 1037
Size: 4025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 890 1028 1850 2282 3029 3297 365
Size: 525 138 822 432 747 268 1093
Fragments arranged by size:
1093 822 747 525 432 268 138
EcoO109I rG'GnC_Cy
Cuts at: 859 879 1164 859
Size: 20 285 3720
Fragments arranged by size:
3720 285 20
EcoRI G'AATT_C
Cuts at: 1029 1029
Size: 4025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 4025
FspI TGC'GCA
Cuts at: 1806 1806
Size: 4025
HaeII r_GCGC'y
Cuts at: 1007 1459 1707 2729 3708 1007
Size: 452 248 1022 979 1324
Fragments arranged by size:
1324 1022 979 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 753 785 1867 1897 1899 1929 753
Size: 32 1082 30 2 30 2849
Fragments arranged by size:
2849 1082 32 30 30 2
HincII GTy'rAC
Cuts at: 1064 1064
Size: 4025
KasI G'GCGC_C
Cuts at: 1455 1703 1455
Size: 248 3777
KpnI G_GTAC'C
Cuts at: 1045 1045
Size: 4025
MfeI C'AATT_G
Cuts at: 548 548
Size: 4025
MluI A'CGCG_T
Cuts at: 3458 3458
Size: 4025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2696 2880 2696
Size: 184 3841
MscI TGG'CCA
Cuts at: 1786 1786
Size: 4025
MslI CAynn'nnrTG
Cuts at: 784 2014 2141 784
Size: 1230 127 2668
Fragments arranged by size:
2668 1230 127
MspA1I CmG'CkG
Cuts at: 1136 1450 1810 2823 3068 1136
Size: 314 360 1013 245 2093
Fragments arranged by size:
2093 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2206 2206
Size: 4025
NarI GG'CG_CC
Cuts at: 1456 1704 1456
Size: 248 3777
NgoMIV G'CCGG_C
Cuts at: 2204 2204
Size: 4025
NheI G'CTAG_C
Cuts at: 754 754
Size: 4025
NspI r_CATG'y
Cuts at: 1078 1578 2485 1078
Size: 500 907 2618
Fragments arranged by size:
2618 907 500
PciI A'CATG_T
Cuts at: 2481 2481
Size: 4025
PmeI GTTT'AAAC
Cuts at: 1024 1024
Size: 4025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3993
PpuMI rG'GwC_Cy
Cuts at: 859 859
Size: 4025
PstI C_TGCA'G
Cuts at: 420 1072 1757 420
Size: 652 685 2688
Fragments arranged by size:
2688 685 652
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4025
PvuII CAG'CTG
Cuts at: 1450 1810 1450
Size: 360 3665
RsrII CG'GwC_CG
Cuts at: 2220 2220
Size: 4025
SacI G_AGCT'C
Cuts at: 1039 1039
Size: 4025
SalI G'TCGA_C
Cuts at: 1062 1062
Size: 4025
SapI GCTCTTCn'nnn_
Cuts at: 1004 2048 2258 1004
Size: 1044 210 2771
Fragments arranged by size:
2771 1044 210
SbfI CC_TGCA'GG
Cuts at: 1072 1072
Size: 4025
ScaI AGT'ACT
Cuts at: 3542 3542
Size: 4025
SexAI A'CCwGG_T
Cuts at: 125 125
Size: 4025
SfcI C'TryA_G
Cuts at: 33 416 725 1068 1753 2746 2937 33
Size: 383 309 343 685 993 191 1121
Fragments arranged by size:
1121 993 685 383 343 309 191
SfoI GGC'GCC
Cuts at: 1457 1705 1457
Size: 248 3777
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
SmaI CCC'GGG
Cuts at: 1047 1047
Size: 4025
SmlI C'TyrA_G
Cuts at: 812 1180 2095 2587 2849 812
Size: 368 915 492 262 1988
Fragments arranged by size:
1988 915 492 368 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4025
SphI G_CATG'C
Cuts at: 1078 1578 1078
Size: 500 3525
StuI AGG'CCT
Cuts at: 571 571
Size: 4025
StyI C'CwwG_G
Cuts at: 1159 1513 1159
Size: 354 3671
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1608 1741 2200 2409 1608
Size: 133 459 209 3224
Fragments arranged by size:
3224 459 209 133
TatI w'GTAC_w
Cuts at: 560 3540 560
Size: 2980 1045
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 3993
Tth111I GACn'n_nGTC
Cuts at: 1822 1822
Size: 4025
XbaI T'CTAG_A
Cuts at: 1056 1056
Size: 4025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1520 1520
Size: 4025
XmaI C'CCGG_G
Cuts at: 1045 1045
Size: 4025
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI