Restriction map for
pF1KB6404
(Circular) (Six-base) MAPSORT of: pF1KB6404.seq Check: 2690 from: 1 to: 4115
pF1KB6404 4115 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1153 1153
Size: 4115
Acc65I G'GTAC_C
Cuts at: 1131 1131
Size: 4115
AclI AA'CG_TT
Cuts at: 3955 3955
Size: 4115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 745 841 874 955 1940 2372 3119 3387
Size: 243 96 33 81 985 432 747 268
Cuts at: 3387 502
Size: 1230
Fragments arranged by size:
1230 985 747 432 268 243 96 81
33
AflIII A'CryG_T
Cuts at: 1467 2571 3548 1467
Size: 1104 977 2034
Fragments arranged by size:
2034 1104 977
AgeI A'CCGG_T
Cuts at: 2503 2503
Size: 4115
AlwNI CAG_nnn'CTG
Cuts at: 723 1420 2987 723
Size: 697 1567 1851
Fragments arranged by size:
1851 1567 697
ApaLI G'TGCA_C
Cuts at: 2885 2885
Size: 4115
ApoI r'AATT_y
Cuts at: 262 317 340 1119 3468 3724 262
Size: 55 23 779 2349 256 653
Fragments arranged by size:
2349 779 653 256 55 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
AvaI C'yCGr_G
Cuts at: 130 459 1135 130
Size: 329 676 3110
Fragments arranged by size:
3110 676 329
BamHI G'GATC_C
Cuts at: 547 621 655 1140 547
Size: 74 34 485 3522
Fragments arranged by size:
3522 485 74 34
BanI G'GyrC_C
Cuts at: 1131 1545 1793 1828 1131
Size: 414 248 35 3418
Fragments arranged by size:
3418 414 248 35
BanII G_rGCy'C
Cuts at: 460 1129 2159 460
Size: 669 1030 2416
Fragments arranged by size:
2416 1030 669
BbeI G_GCGC'C
Cuts at: 1549 1797 1549
Size: 248 3867
BbsI GAAGACnn'nnnn_
Cuts at: 733 862 733
Size: 129 3986
BciVI GTATCCnnnnn_n'
Cuts at: 2013 2774 4115 2013
Size: 761 1341 2013
Fragments arranged by size:
2013 1341 761
BclI T'GATC_A
Cuts at: 1635 1635
Size: 4115
BglII A'GATC_T
Cuts at: 1630 3667 1630
Size: 2037 2078
BlpI GC'TnA_GC
Cuts at: 1227 1227
Size: 4115
Bme1580I G_kGCm'C
Cuts at: 1740 1833 2889 1740
Size: 93 1056 2966
Fragments arranged by size:
2966 1056 93
BmgBI CAC'GTC
Cuts at: 453 453
Size: 4115
BmrI ACTGGGnnnn_n'
Cuts at: 519 1327 1408 1743 3887 519
Size: 808 81 335 2144 747
Fragments arranged by size:
2144 808 747 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 1291 2170 2662 2960 583
Size: 708 879 492 298 1738
Fragments arranged by size:
1738 879 708 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 127 691 3819 127
Size: 564 3128 423
Fragments arranged by size:
3128 564 423
BsaAI yAC'GTr
Cuts at: 1468 1468
Size: 4115
BsaBI GATnn'nnATC
Cuts at: 6 1629 3666 6
Size: 1623 2037 455
Fragments arranged by size:
2037 1623 455
BsaHI Gr'CG_yC
Cuts at: 1546 1794 2496 3992 1546
Size: 248 702 1496 1669
Fragments arranged by size:
1669 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1304 1825 2503 2777 2924 3335 1304
Size: 521 678 274 147 411 2084
Fragments arranged by size:
2084 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 133
Size: 4115
BseYI C'CCAG_C
Cuts at: 369 1540 2298 2875 3371 369
Size: 1171 758 577 496 1113
Fragments arranged by size:
1171 1113 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 635 1657 1703 2911 104
Size: 531 1022 46 1208 1308
Fragments arranged by size:
1308 1208 1022 531 46
BsiHKAI G_wGCw'C
Cuts at: 153 460 1129 1907 2097 2889 153
Size: 307 669 778 190 792 1379
Fragments arranged by size:
1379 792 778 669 307 190
Bsp1286I G_dGCh'C
Cuts at: 153 460 1129 1740 1833 1907 2097 2159 2889
Size: 307 669 611 93 74 190 62 730
Cuts at: 2889 153
Size: 1379
Fragments arranged by size:
1379 730 669 611 307 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3335 3335
Size: 4115
BspHI T'CATG_A
Cuts at: 3291 3291
Size: 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1165 1379 1681 2062 1165
Size: 214 302 381 3218
Fragments arranged by size:
3218 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2407 2461 3945 4110 50
Size: 2357 54 1484 165 55
Fragments arranged by size:
2357 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2027 2027
Size: 4115
BsrFI r'CCGG_y
Cuts at: 2113 2294 2503 2113
Size: 181 209 3725
Fragments arranged by size:
3725 209 181
BsrGI T'GTAC_A
Cuts at: 987 987
Size: 4115
BssSI C'ACGA_G
Cuts at: 2386 2744 2386
Size: 358 3757
BstAPI GCAn_nnn'nTGC
Cuts at: 1492 1492
Size: 4115
BstBI TT'CG_AA
Cuts at: 2476 2476
Size: 4115
BstYI r'GATC_y
Cuts at: 1 547 621 655 1140 1607 1630 1965 2211
Size: 546 74 34 485 467 23 335 246
Cuts at: 2211 3223 3309 3321 3667 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 546 485 467 449 346 335 246
86 74 34 23 12
Bsu36I CC'TnA_GG
Cuts at: 711 711
Size: 4115
BtgI C'CryG_G
Cuts at: 107 107
Size: 4115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1038 2065 2246 2409 1038
Size: 1027 181 163 2744
Fragments arranged by size:
2744 1027 181 163
BtsI GCAGTG_nn'
Cuts at: 254 1358 254
Size: 1104 3011
ClaI AT'CG_AT
Cuts at: 737 737
Size: 4115
DraI TTT'AAA
Cuts at: 1114 1114
Size: 4115
DrdI GACnn_nn'nnGTC
Cuts at: 1821 2679 1821
Size: 858 3257
EaeI y'GGCC_r
Cuts at: 234 632 797 867 1700 1874 2265 2292 3637
Size: 398 165 70 833 174 391 27 1345
Cuts at: 3637 234
Size: 712
Fragments arranged by size:
1345 833 712 398 391 174 165 70
27
EagI C'GGCC_G
Cuts at: 632 1700 632
Size: 1068 3047
EarI CTCTTCn'nnn_
Cuts at: 1093 2138 2348 1093
Size: 1045 210 2860
Fragments arranged by size:
2860 1045 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 162 2323 2633 2779 3925 162
Size: 2161 310 146 1146 352
Fragments arranged by size:
2161 1146 352 310 146
EcoICRI GAG'CTC
Cuts at: 458 1127 458
Size: 669 3446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 745 841 874 955 1940 2372 3119 3387
Size: 243 96 33 81 985 432 747 268
Cuts at: 3387 502
Size: 1230
Fragments arranged by size:
1230 985 747 432 268 243 96 81
33
EcoO109I rG'GnC_Cy
Cuts at: 1254 1254
Size: 4115
EcoRI G'AATT_C
Cuts at: 1119 1119
Size: 4115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 4115
FspI TGC'GCA
Cuts at: 1896 1896
Size: 4115
HaeII r_GCGC'y
Cuts at: 1549 1797 2819 3798 1549
Size: 248 1022 979 1866
Fragments arranged by size:
1866 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 442 474 613 645 694 726 1957 1987 1989
Size: 32 139 32 49 32 1231 30 2
Cuts at: 1989 2019 442
Size: 30 2538
Fragments arranged by size:
2538 1231 139 49 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1154 1154
Size: 4115
KasI G'GCGC_C
Cuts at: 1545 1793 1545
Size: 248 3867
KpnI G_GTAC'C
Cuts at: 1135 1135
Size: 4115
MluI A'CGCG_T
Cuts at: 3548 3548
Size: 4115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 554 2786 2970 554
Size: 2232 184 1699
Fragments arranged by size:
2232 1699 184
MscI TGG'CCA
Cuts at: 236 869 1876 236
Size: 633 1007 2475
Fragments arranged by size:
2475 1007 633
MslI CAynn'nnrTG
Cuts at: 875 1010 2104 2231 875
Size: 135 1094 127 2759
Fragments arranged by size:
2759 1094 135 127
MspA1I CmG'CkG
Cuts at: 1226 1540 1900 2913 3158 1226
Size: 314 360 1013 245 2183
Fragments arranged by size:
2183 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2296 2296
Size: 4115
NarI GG'CG_CC
Cuts at: 1546 1794 1546
Size: 248 3867
NcoI C'CATG_G
Cuts at: 107 107
Size: 4115
NgoMIV G'CCGG_C
Cuts at: 2294 2294
Size: 4115
NspI r_CATG'y
Cuts at: 1168 1668 2575 1168
Size: 500 907 2708
Fragments arranged by size:
2708 907 500
PciI A'CATG_T
Cuts at: 2571 2571
Size: 4115
PmeI GTTT'AAAC
Cuts at: 1114 1114
Size: 4115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 4083
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 772 804 772
Size: 32 4083
PstI C_TGCA'G
Cuts at: 1162 1847 1162
Size: 685 3430
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4115
PvuII CAG'CTG
Cuts at: 1540 1900 1540
Size: 360 3755
RsrII CG'GwC_CG
Cuts at: 2310 2310
Size: 4115
SacI G_AGCT'C
Cuts at: 460 1129 460
Size: 669 3446
SalI G'TCGA_C
Cuts at: 1152 1152
Size: 4115
SapI GCTCTTCn'nnn_
Cuts at: 2138 2348 2138
Size: 210 3905
SbfI CC_TGCA'GG
Cuts at: 1162 1162
Size: 4115
ScaI AGT'ACT
Cuts at: 3632 3632
Size: 4115
SfcI C'TryA_G
Cuts at: 33 834 1158 1843 2836 3027 33
Size: 801 324 685 993 191 1121
Fragments arranged by size:
1121 993 801 685 324 191
SfoI GGC'GCC
Cuts at: 1547 1795 1547
Size: 248 3867
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
SmaI CCC'GGG
Cuts at: 1137 1137
Size: 4115
SmlI C'TyrA_G
Cuts at: 598 1270 2185 2677 2939 598
Size: 672 915 492 262 1774
Fragments arranged by size:
1774 915 672 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4115
SphI G_CATG'C
Cuts at: 1168 1668 1168
Size: 500 3615
StyI C'CwwG_G
Cuts at: 107 1249 1603 107
Size: 1142 354 2619
Fragments arranged by size:
2619 1142 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 151 1052 1698 1831 2290 2499 151
Size: 901 646 133 459 209 1767
Fragments arranged by size:
1767 901 646 459 209 133
TatI w'GTAC_w
Cuts at: 987 3630 987
Size: 2643 1472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 4083
Tth111I GACn'n_nGTC
Cuts at: 1912 1912
Size: 4115
XbaI T'CTAG_A
Cuts at: 1146 1146
Size: 4115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1610 1610
Size: 4115
XmaI C'CCGG_G
Cuts at: 1135 1135
Size: 4115
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI