Restriction map for
pF1KB6406
(Circular) (Six-base) MAPSORT of: pF1KB6406.seq Check: 2156 from: 1 to: 4118
pF1KB6406 4118 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1156 1156
Size: 4118
Acc65I G'GTAC_C
Cuts at: 1134 1134
Size: 4118
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 695 1943 2375 3122 3390 299
Size: 396 1248 432 747 268 1027
Fragments arranged by size:
1248 1027 747 432 396 268
AflIII A'CryG_T
Cuts at: 1470 2574 3551 1470
Size: 1104 977 2037
Fragments arranged by size:
2037 1104 977
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AlwNI CAG_nnn'CTG
Cuts at: 238 1423 2990 238
Size: 1185 1567 1366
Fragments arranged by size:
1567 1366 1185
ApaLI G'TGCA_C
Cuts at: 2888 2888
Size: 4118
ApoI r'AATT_y
Cuts at: 554 1122 3471 3727 554
Size: 568 2349 256 945
Fragments arranged by size:
2349 945 568 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 410 1138 410
Size: 728 3390
BamHI G'GATC_C
Cuts at: 1143 1143
Size: 4118
BanI G'GyrC_C
Cuts at: 123 1134 1548 1796 1831 123
Size: 1011 414 248 35 2410
Fragments arranged by size:
2410 1011 414 248 35
BanII G_rGCy'C
Cuts at: 960 1132 2162 960
Size: 172 1030 2916
Fragments arranged by size:
2916 1030 172
BbeI G_GCGC'C
Cuts at: 127 1552 1800 127
Size: 1425 248 2445
Fragments arranged by size:
2445 1425 248
BciVI GTATCCnnnnn_n'
Cuts at: 2016 2777 4118 2016
Size: 761 1341 2016
Fragments arranged by size:
2016 1341 761
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BglII A'GATC_T
Cuts at: 477 1633 3670 477
Size: 1156 2037 925
Fragments arranged by size:
2037 1156 925
BlpI GC'TnA_GC
Cuts at: 1230 1230
Size: 4118
Bme1580I G_kGCm'C
Cuts at: 1743 1836 2892 1743
Size: 93 1056 2969
Fragments arranged by size:
2969 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 827 935 1330 1411 1746 3890 827
Size: 108 395 81 335 2144 1055
Fragments arranged by size:
2144 1055 395 335 108 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 4118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1040 317
Size: 723 3395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1294 2173 2665 2963 1294
Size: 879 492 298 2449
Fragments arranged by size:
2449 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3822 3822
Size: 4118
BsaAI yAC'GTr
Cuts at: 355 1471 355
Size: 1116 3002
BsaBI GATnn'nnATC
Cuts at: 6 1632 3669 6
Size: 1626 2037 455
Fragments arranged by size:
2037 1626 455
BsaHI Gr'CG_yC
Cuts at: 124 1549 1797 2499 3995 124
Size: 1425 248 702 1496 247
Fragments arranged by size:
1496 1425 702 248 247
BsaWI w'CCGG_w
Cuts at: 1103 1307 1828 2506 2780 2927 3338 1103
Size: 204 521 678 274 147 411 1883
Fragments arranged by size:
1883 678 521 411 274 204 147
BseYI C'CCAG_C
Cuts at: 700 1543 2301 2878 3374 700
Size: 843 758 577 496 1444
Fragments arranged by size:
1444 843 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 680
Size: 4118
BsiEI CG_ry'CG
Cuts at: 104 1660 1706 2914 104
Size: 1556 46 1208 1308
Fragments arranged by size:
1556 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1132 1910 2100 2892 1132
Size: 778 190 792 2358
Fragments arranged by size:
2358 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 960 1132 1743 1836 1910 2100 2162 2892 960
Size: 172 611 93 74 190 62 730 2186
Fragments arranged by size:
2186 730 611 190 172 93 74 62
BspEI T'CCGG_A
Cuts at: 3338 3338
Size: 4118
BspHI T'CATG_A
Cuts at: 3294 3294
Size: 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1168 1382 1684 2065 1168
Size: 214 302 381 3221
Fragments arranged by size:
3221 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 626 2030 626
Size: 1404 2714
BsrFI r'CCGG_y
Cuts at: 2116 2297 2506 2116
Size: 181 209 3728
Fragments arranged by size:
3728 209 181
BssSI C'ACGA_G
Cuts at: 560 2389 2747 560
Size: 1829 358 1931
Fragments arranged by size:
1931 1829 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstYI r'GATC_y
Cuts at: 1 477 1143 1610 1633 1968 2214 3226 3312
Size: 476 666 467 23 335 246 1012 86
Cuts at: 3312 3324 3670 1
Size: 12 346 449
Fragments arranged by size:
1012 666 476 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2068 2249 2412 2068
Size: 181 163 3774
Fragments arranged by size:
3774 181 163
BtsI GCAGTG_nn'
Cuts at: 1361 1361
Size: 4118
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4118
DrdI GACnn_nn'nnGTC
Cuts at: 1824 2682 1824
Size: 858 3260
EaeI y'GGCC_r
Cuts at: 1703 1877 2268 2295 3640 1703
Size: 174 391 27 1345 2181
Fragments arranged by size:
2181 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1703 1703
Size: 4118
EarI CTCTTCn'nnn_
Cuts at: 560 648 2141 2351 560
Size: 88 1493 210 2327
Fragments arranged by size:
2327 1493 210 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2326 2636 2782 3928 2326
Size: 310 146 1146 2516
Fragments arranged by size:
2516 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 317 695 1040 1943 2375 3122 3390 299
Size: 18 378 345 903 432 747 268 1027
Fragments arranged by size:
1027 903 747 432 378 345 268 18
EcoNI CCTnn'n_nnAGG
Cuts at: 299 793 970 299
Size: 494 177 3447
Fragments arranged by size:
3447 494 177
EcoO109I rG'GnC_Cy
Cuts at: 1257 1257
Size: 4118
EcoRI G'AATT_C
Cuts at: 554 1122 554
Size: 568 3550
EcoRV GAT'ATC
Cuts at: 876 876
Size: 4118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 4118
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 127 1552 1800 2822 3801 127
Size: 1425 248 1022 979 444
Fragments arranged by size:
1425 1022 979 444 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 775 807 1960 1990 1992 2022 775
Size: 32 1153 30 2 30 2871
Fragments arranged by size:
2871 1153 32 30 30 2
HincII GTy'rAC
Cuts at: 1157 1157
Size: 4118
KasI G'GCGC_C
Cuts at: 123 1548 1796 123
Size: 1425 248 2445
Fragments arranged by size:
2445 1425 248
KpnI G_GTAC'C
Cuts at: 1138 1138
Size: 4118
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2789 2973 2789
Size: 184 3934
MscI TGG'CCA
Cuts at: 1879 1879
Size: 4118
MslI CAynn'nnrTG
Cuts at: 157 2107 2234 157
Size: 1950 127 2041
Fragments arranged by size:
2041 1950 127
MspA1I CmG'CkG
Cuts at: 1229 1543 1903 2916 3161 1229
Size: 314 360 1013 245 2186
Fragments arranged by size:
2186 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 4118
NarI GG'CG_CC
Cuts at: 124 1549 1797 124
Size: 1425 248 2445
Fragments arranged by size:
2445 1425 248
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 4118
NspI r_CATG'y
Cuts at: 162 1171 1671 2578 162
Size: 1009 500 907 1702
Fragments arranged by size:
1702 1009 907 500
PciI A'CATG_T
Cuts at: 2574 2574
Size: 4118
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 793 825 3257 3289 793
Size: 32 2432 32 1622
Fragments arranged by size:
2432 1622 32 32
PspXI vC'TCGA_Gb
Cuts at: 410 410
Size: 4118
PstI C_TGCA'G
Cuts at: 1165 1850 1165
Size: 685 3433
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 1543 1903 1543
Size: 360 3758
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4118
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SfcI C'TryA_G
Cuts at: 33 632 1161 1846 2839 3030 33
Size: 599 529 685 993 191 1121
Fragments arranged by size:
1121 993 685 599 529 191
SfoI GGC'GCC
Cuts at: 125 1550 1798 125
Size: 1425 248 2445
Fragments arranged by size:
2445 1425 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 1140 1140
Size: 4118
SmlI C'TyrA_G
Cuts at: 410 1273 2188 2680 2942 410
Size: 863 915 492 262 1586
Fragments arranged by size:
1586 915 863 492 262
SnaBI TAC'GTA
Cuts at: 355 355
Size: 4118
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 1171 1671 1171
Size: 500 3618
SspI AAT'ATT
Cuts at: 532 532
Size: 4118
StyI C'CwwG_G
Cuts at: 1007 1097 1252 1606 1007
Size: 90 155 354 3519
Fragments arranged by size:
3519 354 155 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 805 1701 1834 2293 2502 805
Size: 896 133 459 209 2421
Fragments arranged by size:
2421 896 459 209 133
TatI w'GTAC_w
Cuts at: 212 591 992 3633 212
Size: 379 401 2641 697
Fragments arranged by size:
2641 697 401 379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 987 1019 3635 3667 987
Size: 32 2616 32 1438
Fragments arranged by size:
2616 1438 32 32
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 675 1005 1613 675
Size: 330 608 3180
Fragments arranged by size:
3180 608 330
XhoI C'TCGA_G
Cuts at: 410 410
Size: 4118
XmaI C'CCGG_G
Cuts at: 1138 1138
Size: 4118
XmnI GAAnn'nnTTC
Cuts at: 368 368
Size: 4118
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI StuI SwaI
ZraI