Restriction map for
pF1KB6407
(Circular) (Six-base) MAPSORT of: pF1KB6407.seq Check: 3293 from: 1 to: 4031
pF1KB6407 4031 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 614 614
Size: 4031
AccI GT'mk_AC
Cuts at: 1069 1069
Size: 4031
Acc65I G'GTAC_C
Cuts at: 1047 1047
Size: 4031
AclI AA'CG_TT
Cuts at: 3871 3871
Size: 4031
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1856 2288 3035 3303 1000
Size: 856 432 747 268 1728
Fragments arranged by size:
1728 856 747 432 268
AfeI AGC'GCT
Cuts at: 814 814
Size: 4031
AflIII A'CryG_T
Cuts at: 1383 2487 3464 1383
Size: 1104 977 1950
Fragments arranged by size:
1950 1104 977
AgeI A'CCGG_T
Cuts at: 2419 2419
Size: 4031
AleI CACnn'nnGTG
Cuts at: 886 886
Size: 4031
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 904 904
Size: 4031
AlwNI CAG_nnn'CTG
Cuts at: 1336 2903 1336
Size: 1567 2464
ApaLI G'TGCA_C
Cuts at: 2801 2801
Size: 4031
ApoI r'AATT_y
Cuts at: 238 1035 3384 3640 238
Size: 797 2349 256 629
Fragments arranged by size:
2349 797 629 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4031
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
AvaI C'yCGr_G
Cuts at: 169 746 1051 169
Size: 577 305 3149
Fragments arranged by size:
3149 577 305
BamHI G'GATC_C
Cuts at: 152 1056 152
Size: 904 3127
BanI G'GyrC_C
Cuts at: 888 1047 1461 1709 1744 888
Size: 159 414 248 35 3175
Fragments arranged by size:
3175 414 248 159 35
BanII G_rGCy'C
Cuts at: 1045 2075 1045
Size: 1030 3001
BbeI G_GCGC'C
Cuts at: 1465 1713 1465
Size: 248 3783
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 150 184 150
Size: 34 3997
BciVI GTATCCnnnnn_n'
Cuts at: 1929 2690 4031 1929
Size: 761 1341 1929
Fragments arranged by size:
1929 1341 761
BclI T'GATC_A
Cuts at: 1551 1551
Size: 4031
BglII A'GATC_T
Cuts at: 396 1015 1546 3583 396
Size: 619 531 2037 844
Fragments arranged by size:
2037 844 619 531
BlpI GC'TnA_GC
Cuts at: 649 877 1143 649
Size: 228 266 3537
Fragments arranged by size:
3537 266 228
Bme1580I G_kGCm'C
Cuts at: 893 1656 1749 2805 893
Size: 763 93 1056 2119
Fragments arranged by size:
2119 1056 763 93
BmgBI CAC'GTC
Cuts at: 673 673
Size: 4031
BmrI ACTGGGnnnn_n'
Cuts at: 503 1243 1324 1659 3803 503
Size: 740 81 335 2144 731
Fragments arranged by size:
2144 740 731 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 649 2398 649
Size: 1749 2282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 412 829 253
Size: 159 417 3455
Fragments arranged by size:
3455 417 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 1207 2086 2578 2876 421
Size: 786 879 492 298 1576
Fragments arranged by size:
1576 879 786 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3735 3735
Size: 4031
BsaAI yAC'GTr
Cuts at: 147 683 1384 147
Size: 536 701 2794
Fragments arranged by size:
2794 701 536
BsaBI GATnn'nnATC
Cuts at: 6 1545 3582 6
Size: 1539 2037 455
Fragments arranged by size:
2037 1539 455
BsaHI Gr'CG_yC
Cuts at: 165 611 1462 1710 2412 3908 165
Size: 446 851 248 702 1496 288
Fragments arranged by size:
1496 851 702 446 288 248
BsaWI w'CCGG_w
Cuts at: 155 1220 1741 2419 2693 2840 3251 155
Size: 1065 521 678 274 147 411 935
Fragments arranged by size:
1065 935 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 355 385 696 726 355
Size: 30 311 30 3660
Fragments arranged by size:
3660 311 30 30
BseYI C'CCAG_C
Cuts at: 1456 2214 2791 3287 1456
Size: 758 577 496 2200
Fragments arranged by size:
2200 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1573 1619 2827 104
Size: 1469 46 1208 1308
Fragments arranged by size:
1469 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 883 1025 1045 1823 2013 2805 883
Size: 142 20 778 190 792 2109
Fragments arranged by size:
2109 792 778 190 142 20
BsmBI CGTCTCn'nnnn_
Cuts at: 743 743
Size: 4031
Bsp1286I G_dGCh'C
Cuts at: 883 893 1025 1045 1656 1749 1823 2013 2075
Size: 10 132 20 611 93 74 190 62
Cuts at: 2075 2805 883
Size: 730 2109
Fragments arranged by size:
2109 730 611 190 132 93 74 62
20 10
BspEI T'CCGG_A
Cuts at: 155 3251 155
Size: 3096 935
BspHI T'CATG_A
Cuts at: 3207 3207
Size: 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1081 1295 1597 1978 1081
Size: 214 302 381 3134
Fragments arranged by size:
3134 381 302 214
BsrBI CCG'CTC
Cuts at: 50 864 2323 2377 3861 4026 50
Size: 814 1459 54 1484 165 55
Fragments arranged by size:
1484 1459 814 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1943 1943
Size: 4031
BsrFI r'CCGG_y
Cuts at: 2029 2210 2419 2029
Size: 181 209 3641
Fragments arranged by size:
3641 209 181
BsrGI T'GTAC_A
Cuts at: 558 558
Size: 4031
BssSI C'ACGA_G
Cuts at: 808 2302 2660 808
Size: 1494 358 2179
Fragments arranged by size:
2179 1494 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1408 1408
Size: 4031
BstBI TT'CG_AA
Cuts at: 2392 2392
Size: 4031
BstYI r'GATC_y
Cuts at: 1 152 396 1015 1056 1523 1546 1881 2127
Size: 151 244 619 41 467 23 335 246
Cuts at: 2127 3139 3225 3237 3583 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 619 467 449 346 335 246 244
151 86 41 23 12
BtgI C'CryG_G
Cuts at: 107 884 107
Size: 777 3254
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 198 1981 2162 2325 198
Size: 1783 181 163 1904
Fragments arranged by size:
1904 1783 181 163
BtsI GCAGTG_nn'
Cuts at: 1274 1274
Size: 4031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 480 515 480
Size: 35 3996
DraI TTT'AAA
Cuts at: 1030 1030
Size: 4031
DrdI GACnn_nn'nnGTC
Cuts at: 1737 2595 1737
Size: 858 3173
EaeI y'GGCC_r
Cuts at: 488 643 791 1616 1790 2181 2208 3553 488
Size: 155 148 825 174 391 27 1345 966
Fragments arranged by size:
1345 966 825 391 174 155 148 27
EagI C'GGCC_G
Cuts at: 1616 1616
Size: 4031
EarI CTCTTCn'nnn_
Cuts at: 2054 2264 2054
Size: 210 3821
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2239 2549 2695 3841 2239
Size: 310 146 1146 2429
Fragments arranged by size:
2429 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1043 1043
Size: 4031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 412 829 1000 1856 2288 3035 3303 253
Size: 159 417 171 856 432 747 268 981
Fragments arranged by size:
981 856 747 432 417 268 171 159
EcoO109I rG'GnC_Cy
Cuts at: 1170 1170
Size: 4031
EcoRI G'AATT_C
Cuts at: 1035 1035
Size: 4031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 4031
FspI TGC'GCA
Cuts at: 1812 1812
Size: 4031
HaeII r_GCGC'y
Cuts at: 656 816 908 1465 1713 2735 3714 656
Size: 160 92 557 248 1022 979 973
Fragments arranged by size:
1022 979 973 557 248 160 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 355 387 528 560 1873 1903 1905 1935 355
Size: 32 141 32 1313 30 2 30 2451
Fragments arranged by size:
2451 1313 141 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1070 1070
Size: 4031
HindIII A'AGCT_T
Cuts at: 206 982 206
Size: 776 3255
KasI G'GCGC_C
Cuts at: 1461 1709 1461
Size: 248 3783
KpnI G_GTAC'C
Cuts at: 1051 1051
Size: 4031
MluI A'CGCG_T
Cuts at: 3464 3464
Size: 4031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2702 2886 2702
Size: 184 3847
MscI TGG'CCA
Cuts at: 490 793 1792 490
Size: 303 999 2729
Fragments arranged by size:
2729 999 303
MslI CAynn'nnrTG
Cuts at: 886 2020 2147 886
Size: 1134 127 2770
Fragments arranged by size:
2770 1134 127
MspA1I CmG'CkG
Cuts at: 1142 1456 1816 2829 3074 1142
Size: 314 360 1013 245 2099
Fragments arranged by size:
2099 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 4031
NarI GG'CG_CC
Cuts at: 1462 1710 1462
Size: 248 3783
NcoI C'CATG_G
Cuts at: 107 884 107
Size: 777 3254
NdeI CA'TA_TG
Cuts at: 588 588
Size: 4031
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 4031
NspI r_CATG'y
Cuts at: 343 1084 1584 2491 343
Size: 741 500 907 1883
Fragments arranged by size:
1883 907 741 500
PciI A'CATG_T
Cuts at: 2487 2487
Size: 4031
PmeI GTTT'AAAC
Cuts at: 1030 1030
Size: 4031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3999
PstI C_TGCA'G
Cuts at: 1078 1763 1078
Size: 685 3346
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4031
PvuII CAG'CTG
Cuts at: 1456 1816 1456
Size: 360 3671
RsrII CG'GwC_CG
Cuts at: 2226 2226
Size: 4031
SacI G_AGCT'C
Cuts at: 1045 1045
Size: 4031
SalI G'TCGA_C
Cuts at: 1068 1068
Size: 4031
SapI GCTCTTCn'nnn_
Cuts at: 2054 2264 2054
Size: 210 3821
SbfI CC_TGCA'GG
Cuts at: 1078 1078
Size: 4031
ScaI AGT'ACT
Cuts at: 425 3548 425
Size: 3123 908
SfcI C'TryA_G
Cuts at: 33 820 1011 1074 1759 2752 2943 33
Size: 787 191 63 685 993 191 1121
Fragments arranged by size:
1121 993 787 685 191 191 63
SfoI GGC'GCC
Cuts at: 1463 1711 1463
Size: 248 3783
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
SmaI CCC'GGG
Cuts at: 1053 1053
Size: 4031
SmlI C'TyrA_G
Cuts at: 400 1186 2101 2593 2855 400
Size: 786 915 492 262 1576
Fragments arranged by size:
1576 915 786 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4031
SphI G_CATG'C
Cuts at: 1084 1584 1084
Size: 500 3531
StyI C'CwwG_G
Cuts at: 107 468 788 884 1165 1519 107
Size: 361 320 96 281 354 2619
Fragments arranged by size:
2619 361 354 320 281 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1614 1747 2206 2415 1614
Size: 133 459 209 3230
Fragments arranged by size:
3230 459 209 133
TatI w'GTAC_w
Cuts at: 423 558 3546 423
Size: 135 2988 908
Fragments arranged by size:
2988 908 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 696 728 3548 3580 696
Size: 32 2820 32 1147
Fragments arranged by size:
2820 1147 32 32
Tth111I GACn'n_nGTC
Cuts at: 1828 1828
Size: 4031
XbaI T'CTAG_A
Cuts at: 1062 1062
Size: 4031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1526 1526
Size: 4031
XmaI C'CCGG_G
Cuts at: 1051 1051
Size: 4031
ZraI GAC'GTC
Cuts at: 612 612
Size: 4031
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BglI BmtI Bpu10I BseRI BsgI
BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI