FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KB6409, 307 aa 1>>>pF1KB6409 307 - 307 aa - 307 aa Library: /omim/omim.rfq.tfa 60827320 residues in 85289 sequences Statistics: Expectation_n fit: rho(ln(x))= 8.2756+/-0.000327; mu= 2.6277+/- 0.021 mean_var=164.4676+/-34.455, 0's: 0 Z-trim(120.2): 10 B-trim: 2028 in 1/54 Lambda= 0.100008 statistics sampled from 35107 (35117) to 35107 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.761), E-opt: 0.2 (0.412), width: 16 Scan time: 7.590 The best scores are: opt bits E(85289) NP_057569 (OMIM: 610902) vacuolar protein sorting- ( 307) 2047 306.8 3.6e-83 NP_001273300 (OMIM: 610902) vacuolar protein sorti ( 280) 1575 238.6 1.1e-62 NP_001273301 (OMIM: 610902) vacuolar protein sorti ( 222) 1107 171.1 1.9e-42 >>NP_057569 (OMIM: 610902) vacuolar protein sorting-asso (307 aa) initn: 2047 init1: 2047 opt: 2047 Z-score: 1614.1 bits: 306.8 E(85289): 3.6e-83 Smith-Waterman score: 2047; 100.0% identity (100.0% similar) in 307 aa overlap (1-307:1-307) 10 20 30 40 50 60 pF1KB6 MAALAPLPPLPAQFKSIQHHLRTAQEHDKRDPVVAYYCRLYAMQTGMKIDSKTPECRKFL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_057 MAALAPLPPLPAQFKSIQHHLRTAQEHDKRDPVVAYYCRLYAMQTGMKIDSKTPECRKFL 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB6 SKLMDQLEALKKQLGDNEAITQEIVGCAHLENYALKMFLYADNEDRAGRFHKNMIKSFYT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_057 SKLMDQLEALKKQLGDNEAITQEIVGCAHLENYALKMFLYADNEDRAGRFHKNMIKSFYT 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB6 ASLLIDVITVFGELTDENVKHRKYARWKATYIHNCLKNGETPQAGPVGIEEDNDIEENED :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_057 ASLLIDVITVFGELTDENVKHRKYARWKATYIHNCLKNGETPQAGPVGIEEDNDIEENED 130 140 150 160 170 180 190 200 210 220 230 240 pF1KB6 AGAASLPTQPTQPSSSSTYDPSNMPSGNYTGIQIPPGAHAPANTPAEVPHSTGVASNTIQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_057 AGAASLPTQPTQPSSSSTYDPSNMPSGNYTGIQIPPGAHAPANTPAEVPHSTGVASNTIQ 190 200 210 220 230 240 250 260 270 280 290 300 pF1KB6 PTPQTIPAIDPALFNTISQGDVRLTPEDFARAQKYCKYAGSALQYEDVSTAVQNLQKALK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_057 PTPQTIPAIDPALFNTISQGDVRLTPEDFARAQKYCKYAGSALQYEDVSTAVQNLQKALK 250 260 270 280 290 300 pF1KB6 LLTTGRE ::::::: NP_057 LLTTGRE >>NP_001273300 (OMIM: 610902) vacuolar protein sorting-a (280 aa) initn: 1575 init1: 1575 opt: 1575 Z-score: 1246.6 bits: 238.6 E(85289): 1.1e-62 Smith-Waterman score: 1809; 91.2% identity (91.2% similar) in 307 aa overlap (1-307:1-280) 10 20 30 40 50 60 pF1KB6 MAALAPLPPLPAQFKSIQHHLRTAQEHDKRDPVVAYYCRLYAMQTGMKIDSKTPECRKFL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 MAALAPLPPLPAQFKSIQHHLRTAQEHDKRDPVVAYYCRLYAMQTGMKIDSKTPECRKFL 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB6 SKLMDQLEALKKQLGDNEAITQEIVGCAHLENYALKMFLYADNEDRAGRFHKNMIKSFYT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 SKLMDQLEALKKQLGDNEAITQEIVGCAHLENYALKMFLYADNEDRAGRFHKNMIKSFYT 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB6 ASLLIDVITVFGELTDENVKHRKYARWKATYIHNCLKNGETPQAGPVGIEEDNDIEENED :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 ASLLIDVITVFGELTDENVKHRKYARWKATYIHNCLKNGETPQAGPVGIEEDNDIEENED 130 140 150 160 170 180 190 200 210 220 230 240 pF1KB6 AGAASLPTQPTQPSSSSTYDPSNMPSGNYTGIQIPPGAHAPANTPAEVPHSTGVASNTIQ ::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 AGAASLPTQPTQPSSSSTYDPSNMPSGNYTGIQIPPGAHAPANTPAEVPHSTG------- 190 200 210 220 230 250 260 270 280 290 300 pF1KB6 PTPQTIPAIDPALFNTISQGDVRLTPEDFARAQKYCKYAGSALQYEDVSTAVQNLQKALK :::::::::::::::::::::::::::::::::::::::: NP_001 --------------------DVRLTPEDFARAQKYCKYAGSALQYEDVSTAVQNLQKALK 240 250 260 270 pF1KB6 LLTTGRE ::::::: NP_001 LLTTGRE 280 >>NP_001273301 (OMIM: 610902) vacuolar protein sorting-a (222 aa) initn: 1105 init1: 1105 opt: 1107 Z-score: 883.2 bits: 171.1 E(85289): 1.9e-42 Smith-Waterman score: 1341; 86.2% identity (87.4% similar) in 247 aa overlap (61-307:3-222) 40 50 60 70 80 90 pF1KB6 DPVVAYYCRLYAMQTGMKIDSKTPECRKFLSKLMDQLEALKKQLGDNEAITQEIVGCAHL :. . : .::::::::::::::::::::: NP_001 MTSETLWWLITLKKQLGDNEAITQEIVGCAHL 10 20 30 100 110 120 130 140 150 pF1KB6 ENYALKMFLYADNEDRAGRFHKNMIKSFYTASLLIDVITVFGELTDENVKHRKYARWKAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 ENYALKMFLYADNEDRAGRFHKNMIKSFYTASLLIDVITVFGELTDENVKHRKYARWKAT 40 50 60 70 80 90 160 170 180 190 200 210 pF1KB6 YIHNCLKNGETPQAGPVGIEEDNDIEENEDAGAASLPTQPTQPSSSSTYDPSNMPSGNYT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 YIHNCLKNGETPQAGPVGIEEDNDIEENEDAGAASLPTQPTQPSSSSTYDPSNMPSGNYT 100 110 120 130 140 150 220 230 240 250 260 270 pF1KB6 GIQIPPGAHAPANTPAEVPHSTGVASNTIQPTPQTIPAIDPALFNTISQGDVRLTPEDFA ::::::::::::::::::::::: :::::::::: NP_001 GIQIPPGAHAPANTPAEVPHSTG---------------------------DVRLTPEDFA 160 170 180 280 290 300 pF1KB6 RAQKYCKYAGSALQYEDVSTAVQNLQKALKLLTTGRE ::::::::::::::::::::::::::::::::::::: NP_001 RAQKYCKYAGSALQYEDVSTAVQNLQKALKLLTTGRE 190 200 210 220 307 residues in 1 query sequences 60827320 residues in 85289 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Fri Nov 4 22:53:25 2016 done: Fri Nov 4 22:53:26 2016 Total Scan time: 7.590 Total Display time: -0.030 Function used was FASTA [36.3.4 Apr, 2011]