Restriction map for
pF1KB6409
(Circular) (Six-base) MAPSORT of: pF1KB6409.seq Check: 8568 from: 1 to: 4034
pF1KB6409 4034 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1072 1072
Size: 4034
Acc65I G'GTAC_C
Cuts at: 1050 1050
Size: 4034
AclI AA'CG_TT
Cuts at: 3874 3874
Size: 4034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1859 2291 3038 3306 1859
Size: 432 747 268 2587
Fragments arranged by size:
2587 747 432 268
AflIII A'CryG_T
Cuts at: 1386 2490 3467 1386
Size: 1104 977 1953
Fragments arranged by size:
1953 1104 977
AgeI A'CCGG_T
Cuts at: 2422 2422
Size: 4034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 970 970
Size: 4034
AlwNI CAG_nnn'CTG
Cuts at: 604 1339 2906 604
Size: 735 1567 1732
Fragments arranged by size:
1732 1567 735
ApaLI G'TGCA_C
Cuts at: 758 2804 758
Size: 2046 1988
ApoI r'AATT_y
Cuts at: 280 1038 3387 3643 280
Size: 758 2349 256 671
Fragments arranged by size:
2349 758 671 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
AvaI C'yCGr_G
Cuts at: 1054 1054
Size: 4034
BamHI G'GATC_C
Cuts at: 1059 1059
Size: 4034
BanI G'GyrC_C
Cuts at: 1050 1464 1712 1747 1050
Size: 414 248 35 3337
Fragments arranged by size:
3337 414 248 35
BanII G_rGCy'C
Cuts at: 924 1048 2078 924
Size: 124 1030 2880
Fragments arranged by size:
2880 1030 124
BbeI G_GCGC'C
Cuts at: 1468 1716 1468
Size: 248 3786
BbsI GAAGACnn'nnnn_
Cuts at: 914 914
Size: 4034
BciVI GTATCCnnnnn_n'
Cuts at: 1932 2693 4034 1932
Size: 761 1341 1932
Fragments arranged by size:
1932 1341 761
BclI T'GATC_A
Cuts at: 449 1554 449
Size: 1105 2929
BglII A'GATC_T
Cuts at: 1549 3586 1549
Size: 2037 1997
BlpI GC'TnA_GC
Cuts at: 1146 1146
Size: 4034
Bme1580I G_kGCm'C
Cuts at: 372 762 1659 1752 2808 372
Size: 390 897 93 1056 1598
Fragments arranged by size:
1598 1056 897 390 93
BmrI ACTGGGnnnn_n'
Cuts at: 1246 1327 1662 3806 1246
Size: 81 335 2144 1474
Fragments arranged by size:
2144 1474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 194 2401 194
Size: 2207 1827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 752 671
Size: 81 3953
Bpu10I CC'TnA_GC
Cuts at: 179 179
Size: 4034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 578 988 1210 2089 2581 2879 335
Size: 243 410 222 879 492 298 1490
Fragments arranged by size:
1490 879 492 410 298 243 222
BsaI GGTCTCn'nnnn_
Cuts at: 191 3738 191
Size: 3547 487
BsaAI yAC'GTr
Cuts at: 1387 1387
Size: 4034
BsaBI GATnn'nnATC
Cuts at: 6 1548 3585 6
Size: 1542 2037 455
Fragments arranged by size:
2037 1542 455
BsaHI Gr'CG_yC
Cuts at: 1465 1713 2415 3911 1465
Size: 248 702 1496 1588
Fragments arranged by size:
1588 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1223 1744 2422 2696 2843 3254 1223
Size: 521 678 274 147 411 2003
Fragments arranged by size:
2003 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 581 581
Size: 4034
BseYI C'CCAG_C
Cuts at: 1459 2217 2794 3290 1459
Size: 758 577 496 2203
Fragments arranged by size:
2203 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1576 1622 2830 104
Size: 1472 46 1208 1308
Fragments arranged by size:
1472 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 762 924 1048 1826 2016 2808 762
Size: 162 124 778 190 792 1988
Fragments arranged by size:
1988 792 778 190 162 124
Bsp1286I G_dGCh'C
Cuts at: 372 762 924 1048 1659 1752 1826 2016 2078
Size: 390 162 124 611 93 74 190 62
Cuts at: 2078 2808 372
Size: 730 1598
Fragments arranged by size:
1598 730 611 390 190 162 124 93
74 62
BspEI T'CCGG_A
Cuts at: 3254 3254
Size: 4034
BspHI T'CATG_A
Cuts at: 3210 3210
Size: 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 855 1084 1298 1600 1981 855
Size: 229 214 302 381 2908
Fragments arranged by size:
2908 381 302 229 214
BsrBI CCG'CTC
Cuts at: 50 135 2326 2380 3864 4029 50
Size: 85 2191 54 1484 165 55
Fragments arranged by size:
2191 1484 165 85 55 54
BsrDI GCAATG_nn'
Cuts at: 239 1946 239
Size: 1707 2327
BsrFI r'CCGG_y
Cuts at: 2032 2213 2422 2032
Size: 181 209 3644
Fragments arranged by size:
3644 209 181
BssSI C'ACGA_G
Cuts at: 2305 2663 2305
Size: 358 3676
BstAPI GCAn_nnn'nTGC
Cuts at: 1411 1411
Size: 4034
BstBI TT'CG_AA
Cuts at: 2395 2395
Size: 4034
BstYI r'GATC_y
Cuts at: 1 1059 1526 1549 1884 2130 3142 3228 3240
Size: 1058 467 23 335 246 1012 86 12
Cuts at: 3240 3586 1
Size: 346 449
Fragments arranged by size:
1058 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4034
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1984 2165 2328 1984
Size: 181 163 3690
Fragments arranged by size:
3690 181 163
BtsI GCAGTG_nn'
Cuts at: 954 974 1277 954
Size: 20 303 3711
Fragments arranged by size:
3711 303 20
ClaI AT'CG_AT
Cuts at: 254 254
Size: 4034
DraI TTT'AAA
Cuts at: 575 1033 575
Size: 458 3576
DrdI GACnn_nn'nnGTC
Cuts at: 1740 2598 1740
Size: 858 3176
EaeI y'GGCC_r
Cuts at: 110 1619 1793 2184 2211 3556 110
Size: 1509 174 391 27 1345 588
Fragments arranged by size:
1509 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1619 1619
Size: 4034
EarI CTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2242 2552 2698 3844 2242
Size: 310 146 1146 2432
Fragments arranged by size:
2432 1146 310 146
EcoICRI GAG'CTC
Cuts at: 922 1046 922
Size: 124 3910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 752 1859 2291 3038 3306 671
Size: 81 1107 432 747 268 1399
Fragments arranged by size:
1399 1107 747 432 268 81
EcoNI CCTnn'n_nnAGG
Cuts at: 596 596
Size: 4034
EcoO109I rG'GnC_Cy
Cuts at: 601 1173 601
Size: 572 3462
EcoRI G'AATT_C
Cuts at: 1038 1038
Size: 4034
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 4034
FspI TGC'GCA
Cuts at: 1815 1815
Size: 4034
HaeII r_GCGC'y
Cuts at: 1468 1716 2738 3717 1468
Size: 248 1022 979 1785
Fragments arranged by size:
1785 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1876 1906 1908 1938 1876
Size: 30 2 30 3972
Fragments arranged by size:
3972 30 30 2
HincII GTy'rAC
Cuts at: 1073 1073
Size: 4034
KasI G'GCGC_C
Cuts at: 1464 1712 1464
Size: 248 3786
KpnI G_GTAC'C
Cuts at: 1054 1054
Size: 4034
MluI A'CGCG_T
Cuts at: 3467 3467
Size: 4034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 588 849 2705 2889 588
Size: 261 1856 184 1733
Fragments arranged by size:
1856 1733 261 184
MscI TGG'CCA
Cuts at: 1795 1795
Size: 4034
MslI CAynn'nnrTG
Cuts at: 2023 2150 2023
Size: 127 3907
MspA1I CmG'CkG
Cuts at: 126 1145 1459 1819 2832 3077 126
Size: 1019 314 360 1013 245 1083
Fragments arranged by size:
1083 1019 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2215 2215
Size: 4034
NarI GG'CG_CC
Cuts at: 1465 1713 1465
Size: 248 3786
NcoI C'CATG_G
Cuts at: 107 107
Size: 4034
NgoMIV G'CCGG_C
Cuts at: 2213 2213
Size: 4034
NspI r_CATG'y
Cuts at: 720 1087 1587 2494 720
Size: 367 500 907 2260
Fragments arranged by size:
2260 907 500 367
PasI CC'CwG_GG
Cuts at: 882 882
Size: 4034
PciI A'CATG_T
Cuts at: 2490 2490
Size: 4034
PmeI GTTT'AAAC
Cuts at: 1033 1033
Size: 4034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4002
PstI C_TGCA'G
Cuts at: 1081 1766 1081
Size: 685 3349
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4034
PvuII CAG'CTG
Cuts at: 1459 1819 1459
Size: 360 3674
RsrII CG'GwC_CG
Cuts at: 2229 2229
Size: 4034
SacI G_AGCT'C
Cuts at: 924 1048 924
Size: 124 3910
SalI G'TCGA_C
Cuts at: 1071 1071
Size: 4034
SapI GCTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
SbfI CC_TGCA'GG
Cuts at: 1081 1081
Size: 4034
ScaI AGT'ACT
Cuts at: 931 3551 931
Size: 2620 1414
SfcI C'TryA_G
Cuts at: 33 1077 1762 2755 2946 33
Size: 1044 685 993 191 1121
Fragments arranged by size:
1121 1044 993 685 191
SfoI GGC'GCC
Cuts at: 1466 1714 1466
Size: 248 3786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
SmaI CCC'GGG
Cuts at: 1056 1056
Size: 4034
SmlI C'TyrA_G
Cuts at: 350 593 1003 1189 2104 2596 2858 350
Size: 243 410 186 915 492 262 1526
Fragments arranged by size:
1526 915 492 410 262 243 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4034
SphI G_CATG'C
Cuts at: 1087 1587 1087
Size: 500 3534
StyI C'CwwG_G
Cuts at: 107 1168 1522 107
Size: 1061 354 2619
Fragments arranged by size:
2619 1061 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 319 1617 1750 2209 2418 319
Size: 1298 133 459 209 1935
Fragments arranged by size:
1935 1298 459 209 133
TatI w'GTAC_w
Cuts at: 929 3549 929
Size: 2620 1414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4002
Tth111I GACn'n_nGTC
Cuts at: 1831 1831
Size: 4034
XbaI T'CTAG_A
Cuts at: 1065 1065
Size: 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1529 1529
Size: 4034
XmaI C'CCGG_G
Cuts at: 1054 1054
Size: 4034
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI