Restriction map for
pF1KB6411
(Circular) (Six-base) MAPSORT of: pF1KB6411.seq Check: 4199 from: 1 to: 4034
pF1KB6411 4034 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AatII G_ACGT'C
Cuts at: 323 323
Size: 4034
AccI GT'mk_AC
Cuts at: 1072 1072
Size: 4034
Acc65I G'GTAC_C
Cuts at: 1050 1050
Size: 4034
AclI AA'CG_TT
Cuts at: 385 3874 385
Size: 3489 545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1859 2291 3038 3306 1859
Size: 432 747 268 2587
Fragments arranged by size:
2587 747 432 268
AflII C'TTAA_G
Cuts at: 406 406
Size: 4034
AflIII A'CryG_T
Cuts at: 1386 2490 3467 1386
Size: 1104 977 1953
Fragments arranged by size:
1953 1104 977
AgeI A'CCGG_T
Cuts at: 2422 2422
Size: 4034
AlwNI CAG_nnn'CTG
Cuts at: 627 1339 2906 627
Size: 712 1567 1755
Fragments arranged by size:
1755 1567 712
ApaI G_GGCC'C
Cuts at: 680 680
Size: 4034
ApaLI G'TGCA_C
Cuts at: 595 2804 595
Size: 2209 1825
ApoI r'AATT_y
Cuts at: 1038 3387 3643 1038
Size: 2349 256 1429
Fragments arranged by size:
2349 1429 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
AvaI C'yCGr_G
Cuts at: 737 1054 737
Size: 317 3717
BamHI G'GATC_C
Cuts at: 1059 1059
Size: 4034
BanI G'GyrC_C
Cuts at: 663 882 1050 1464 1712 1747 663
Size: 219 168 414 248 35 2950
Fragments arranged by size:
2950 414 248 219 168 35
BanII G_rGCy'C
Cuts at: 164 680 736 1048 2078 164
Size: 516 56 312 1030 2120
Fragments arranged by size:
2120 1030 516 312 56
BbeI G_GCGC'C
Cuts at: 1468 1716 1468
Size: 248 3786
BbsI GAAGACnn'nnnn_
Cuts at: 716 716
Size: 4034
BbvCI CC'TCA_GC
Cuts at: 556 556
Size: 4034
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 133 167 133
Size: 34 4000
BciVI GTATCCnnnnn_n'
Cuts at: 1932 2693 4034 1932
Size: 761 1341 1932
Fragments arranged by size:
1932 1341 761
BclI T'GATC_A
Cuts at: 1554 1554
Size: 4034
BglII A'GATC_T
Cuts at: 209 542 584 1549 3586 209
Size: 333 42 965 2037 657
Fragments arranged by size:
2037 965 657 333 42
BlpI GC'TnA_GC
Cuts at: 1146 1146
Size: 4034
Bme1580I G_kGCm'C
Cuts at: 599 680 819 1659 1752 2808 599
Size: 81 139 840 93 1056 1825
Fragments arranged by size:
1825 1056 840 139 93 81
BmgBI CAC'GTC
Cuts at: 768 768
Size: 4034
BmrI ACTGGGnnnn_n'
Cuts at: 771 1246 1327 1662 3806 771
Size: 475 81 335 2144 999
Fragments arranged by size:
2144 999 475 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 928
Size: 4034
Bpu10I CC'TnA_GC
Cuts at: 197 556 197
Size: 359 3675
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 2089 2581 2879 1210
Size: 879 492 298 2365
Fragments arranged by size:
2365 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 322 3738 322
Size: 3416 618
BsaAI yAC'GTr
Cuts at: 1387 1387
Size: 4034
BsaBI GATnn'nnATC
Cuts at: 6 1548 3585 6
Size: 1542 2037 455
Fragments arranged by size:
2037 1542 455
BsaHI Gr'CG_yC
Cuts at: 320 1465 1713 2415 3911 320
Size: 1145 248 702 1496 443
Fragments arranged by size:
1496 1145 702 443 248
BsaWI w'CCGG_w
Cuts at: 1223 1744 2422 2696 2843 3254 1223
Size: 521 678 274 147 411 2003
Fragments arranged by size:
2003 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 682 755 235
Size: 447 73 3514
Fragments arranged by size:
3514 447 73
BseYI C'CCAG_C
Cuts at: 722 1459 2217 2794 3290 722
Size: 737 758 577 496 1466
Fragments arranged by size:
1466 758 737 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 519 253
Size: 266 3768
BsiEI CG_ry'CG
Cuts at: 104 651 1576 1622 2830 104
Size: 547 925 46 1208 1308
Fragments arranged by size:
1308 1208 925 547 46
BsiHKAI G_wGCw'C
Cuts at: 164 599 869 1048 1826 2016 2808 164
Size: 435 270 179 778 190 792 1390
Fragments arranged by size:
1390 792 778 435 270 190 179
BsmBI CGTCTCn'nnnn_
Cuts at: 853 853
Size: 4034
Bsp1286I G_dGCh'C
Cuts at: 164 599 680 736 819 869 1048 1659 1752
Size: 435 81 56 83 50 179 611 93
Cuts at: 1752 1826 2016 2078 2808 164
Size: 74 190 62 730 1390
Fragments arranged by size:
1390 730 611 435 190 179 93 83
81 74 62 56 50
BspEI T'CCGG_A
Cuts at: 3254 3254
Size: 4034
BspHI T'CATG_A
Cuts at: 669 3210 669
Size: 2541 1493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 462 1084 1298 1600 1981 462
Size: 622 214 302 381 2515
Fragments arranged by size:
2515 622 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2326 2380 3864 4029 50
Size: 2276 54 1484 165 55
Fragments arranged by size:
2276 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1946 1946
Size: 4034
BsrFI r'CCGG_y
Cuts at: 131 745 2032 2213 2422 131
Size: 614 1287 181 209 1743
Fragments arranged by size:
1743 1287 614 209 181
BssSI C'ACGA_G
Cuts at: 2305 2663 2305
Size: 358 3676
BstAPI GCAn_nnn'nTGC
Cuts at: 663 1411 663
Size: 748 3286
BstBI TT'CG_AA
Cuts at: 2395 2395
Size: 4034
BstYI r'GATC_y
Cuts at: 1 209 542 584 1059 1526 1549 1884 2130
Size: 208 333 42 475 467 23 335 246
Cuts at: 2130 3142 3228 3240 3586 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 475 467 449 346 335 333 246
208 86 42 23 12
BtgI C'CryG_G
Cuts at: 107 273 363 1010 107
Size: 166 90 647 3131
Fragments arranged by size:
3131 647 166 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1984 2165 2328 1984
Size: 181 163 3690
Fragments arranged by size:
3690 181 163
BtsI GCAGTG_nn'
Cuts at: 768 1277 768
Size: 509 3525
ClaI AT'CG_AT
Cuts at: 575 575
Size: 4034
DraI TTT'AAA
Cuts at: 1033 1033
Size: 4034
DraIII CAC_nnn'GTG
Cuts at: 260 875 260
Size: 615 3419
DrdI GACnn_nn'nnGTC
Cuts at: 250 1740 2598 250
Size: 1490 858 1686
Fragments arranged by size:
1686 1490 858
EaeI y'GGCC_r
Cuts at: 1013 1619 1793 2184 2211 3556 1013
Size: 606 174 391 27 1345 1491
Fragments arranged by size:
1491 1345 606 391 174 27
EagI C'GGCC_G
Cuts at: 1619 1619
Size: 4034
EarI CTCTTCn'nnn_
Cuts at: 346 2057 2267 346
Size: 1711 210 2113
Fragments arranged by size:
2113 1711 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2242 2552 2698 3844 127
Size: 2115 310 146 1146 317
Fragments arranged by size:
2115 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 162 1046 162
Size: 884 3150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1859 2291 3038 3306 928
Size: 931 432 747 268 1656
Fragments arranged by size:
1656 931 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 186 603 1173 186
Size: 417 570 3047
Fragments arranged by size:
3047 570 417
EcoRI G'AATT_C
Cuts at: 1038 1038
Size: 4034
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 867 2053 867
Size: 1186 2848
FspI TGC'GCA
Cuts at: 502 1815 502
Size: 1313 2721
HaeII r_GCGC'y
Cuts at: 531 1468 1716 2738 3717 531
Size: 937 248 1022 979 848
Fragments arranged by size:
1022 979 937 848 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 615 647 1876 1906 1908 1938 615
Size: 32 1229 30 2 30 2711
Fragments arranged by size:
2711 1229 32 30 30 2
HincII GTy'rAC
Cuts at: 1073 1073
Size: 4034
KasI G'GCGC_C
Cuts at: 1464 1712 1464
Size: 248 3786
KpnI G_GTAC'C
Cuts at: 1054 1054
Size: 4034
MluI A'CGCG_T
Cuts at: 3467 3467
Size: 4034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2705 2889 2705
Size: 184 3850
MscI TGG'CCA
Cuts at: 1795 1795
Size: 4034
MslI CAynn'nnrTG
Cuts at: 802 854 2023 2150 802
Size: 52 1169 127 2686
Fragments arranged by size:
2686 1169 127 52
MspA1I CmG'CkG
Cuts at: 147 902 1145 1459 1819 2832 3077 147
Size: 755 243 314 360 1013 245 1104
Fragments arranged by size:
1104 1013 755 360 314 245 243
NaeI GCC'GGC
Cuts at: 747 2215 747
Size: 1468 2566
NarI GG'CG_CC
Cuts at: 1465 1713 1465
Size: 248 3786
NcoI C'CATG_G
Cuts at: 107 273 107
Size: 166 3868
NgoMIV G'CCGG_C
Cuts at: 745 2213 745
Size: 1468 2566
NspI r_CATG'y
Cuts at: 1087 1587 2494 1087
Size: 500 907 2627
Fragments arranged by size:
2627 907 500
PciI A'CATG_T
Cuts at: 2490 2490
Size: 4034
PflMI CCAn_nnn'nTGG
Cuts at: 627 722 825 627
Size: 95 103 3836
Fragments arranged by size:
3836 103 95
PmeI GTTT'AAAC
Cuts at: 1033 1033
Size: 4034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4002
PspOMI G'GGCC_C
Cuts at: 676 676
Size: 4034
PstI C_TGCA'G
Cuts at: 1081 1766 1081
Size: 685 3349
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4034
PvuII CAG'CTG
Cuts at: 147 1459 1819 147
Size: 1312 360 2362
Fragments arranged by size:
2362 1312 360
RsrII CG'GwC_CG
Cuts at: 2229 2229
Size: 4034
SacI G_AGCT'C
Cuts at: 164 1048 164
Size: 884 3150
SalI G'TCGA_C
Cuts at: 1071 1071
Size: 4034
SapI GCTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
SbfI CC_TGCA'GG
Cuts at: 1081 1081
Size: 4034
ScaI AGT'ACT
Cuts at: 3551 3551
Size: 4034
SfcI C'TryA_G
Cuts at: 33 372 1077 1762 2755 2946 33
Size: 339 705 685 993 191 1121
Fragments arranged by size:
1121 993 705 685 339 191
SfoI GGC'GCC
Cuts at: 1466 1714 1466
Size: 248 3786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
SmaI CCC'GGG
Cuts at: 1056 1056
Size: 4034
SmlI C'TyrA_G
Cuts at: 406 1189 2104 2596 2858 406
Size: 783 915 492 262 1582
Fragments arranged by size:
1582 915 783 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4034
SphI G_CATG'C
Cuts at: 1087 1587 1087
Size: 500 3534
StyI C'CwwG_G
Cuts at: 107 273 1168 1522 107
Size: 166 895 354 2619
Fragments arranged by size:
2619 895 354 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 665 900 1617 1750 2209 2418 665
Size: 235 717 133 459 209 2281
Fragments arranged by size:
2281 717 459 235 209 133
TatI w'GTAC_w
Cuts at: 3549 3549
Size: 4034
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 977 1009 3551 3583 977
Size: 32 2542 32 1428
Fragments arranged by size:
2542 1428 32 32
Tth111I GACn'n_nGTC
Cuts at: 1831 1831
Size: 4034
XbaI T'CTAG_A
Cuts at: 1065 1065
Size: 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 210 624 927 1008 1529 210
Size: 414 303 81 521 2715
Fragments arranged by size:
2715 521 414 303 81
XmaI C'CCGG_G
Cuts at: 1054 1054
Size: 4034
XmnI GAAnn'nnTTC
Cuts at: 952 952
Size: 4034
ZraI GAC'GTC
Cuts at: 321 321
Size: 4034
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BfrBI BglI BmtI BsaXI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI