Restriction map for
pF1KB6413
(Circular) (Six-base) MAPSORT of: pF1KB6413.seq Check: 8999 from: 1 to: 4037
pF1KB6413 4037 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 615 615
Size: 4037
AatII G_ACGT'C
Cuts at: 683 683
Size: 4037
AccI GT'mk_AC
Cuts at: 977 1075 977
Size: 98 3939
Acc65I G'GTAC_C
Cuts at: 1053 1053
Size: 4037
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 1008 1862 2294 3041 3309 397
Size: 611 854 432 747 268 1125
Fragments arranged by size:
1125 854 747 611 432 268
AflIII A'CryG_T
Cuts at: 437 1389 2493 3470 437
Size: 952 1104 977 1004
Fragments arranged by size:
1104 1004 977 952
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AlwNI CAG_nnn'CTG
Cuts at: 921 1342 2909 921
Size: 421 1567 2049
Fragments arranged by size:
2049 1567 421
ApaI G_GGCC'C
Cuts at: 693 693
Size: 4037
ApaLI G'TGCA_C
Cuts at: 2807 2807
Size: 4037
ApoI r'AATT_y
Cuts at: 1041 3390 3646 1041
Size: 2349 256 1432
Fragments arranged by size:
2349 1432 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 529 1057 529
Size: 528 3509
BamHI G'GATC_C
Cuts at: 1062 1062
Size: 4037
BanI G'GyrC_C
Cuts at: 188 813 1053 1467 1715 1750 188
Size: 625 240 414 248 35 2475
Fragments arranged by size:
2475 625 414 248 240 35
BanII G_rGCy'C
Cuts at: 151 693 1051 2081 151
Size: 542 358 1030 2107
Fragments arranged by size:
2107 1030 542 358
BbeI G_GCGC'C
Cuts at: 1471 1719 1471
Size: 248 3789
BciVI GTATCCnnnnn_n'
Cuts at: 1935 2696 4037 1935
Size: 761 1341 1935
Fragments arranged by size:
1935 1341 761
BclI T'GATC_A
Cuts at: 1557 1557
Size: 4037
BfrBI ATG'CAT
Cuts at: 951 951
Size: 4037
BglII A'GATC_T
Cuts at: 1552 3589 1552
Size: 2037 2000
BlpI GC'TnA_GC
Cuts at: 1149 1149
Size: 4037
Bme1580I G_kGCm'C
Cuts at: 693 1662 1755 2811 693
Size: 969 93 1056 1919
Fragments arranged by size:
1919 1056 969 93
BmrI ACTGGGnnnn_n'
Cuts at: 743 891 954 990 1249 1330 1665 3809 743
Size: 148 63 36 259 81 335 2144 971
Fragments arranged by size:
2144 971 335 259 148 81 63 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4037
Bpu10I CC'TnA_GC
Cuts at: 341 764 341
Size: 423 3614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 241 852 1213 2092 2584 2882 103
Size: 138 611 361 879 492 298 1258
Fragments arranged by size:
1258 879 611 492 361 298 138
BsaI GGTCTCn'nnnn_
Cuts at: 3741 3741
Size: 4037
BsaAI yAC'GTr
Cuts at: 1390 1390
Size: 4037
BsaBI GATnn'nnATC
Cuts at: 6 1551 3588 6
Size: 1545 2037 455
Fragments arranged by size:
2037 1545 455
BsaHI Gr'CG_yC
Cuts at: 680 1468 1716 2418 3914 680
Size: 788 248 702 1496 803
Fragments arranged by size:
1496 803 788 702 248
BsaWI w'CCGG_w
Cuts at: 143 1226 1747 2425 2699 2846 3257 143
Size: 1083 521 678 274 147 411 923
Fragments arranged by size:
1083 923 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 439 469 665 695 439
Size: 30 196 30 3781
Fragments arranged by size:
3781 196 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 769 313
Size: 456 3581
BseYI C'CCAG_C
Cuts at: 151 170 1462 2220 2797 3293 151
Size: 19 1292 758 577 496 895
Fragments arranged by size:
1292 895 758 577 496 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 534 687 534
Size: 153 3884
BsiEI CG_ry'CG
Cuts at: 104 311 1579 1625 2833 104
Size: 207 1268 46 1208 1308
Fragments arranged by size:
1308 1268 1208 207 46
BsiHKAI G_wGCw'C
Cuts at: 1051 1829 2019 2811 1051
Size: 778 190 792 2277
Fragments arranged by size:
2277 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 220 220
Size: 4037
Bsp1286I G_dGCh'C
Cuts at: 151 693 1051 1662 1755 1829 2019 2081 2811
Size: 542 358 611 93 74 190 62 730
Cuts at: 2811 151
Size: 1377
Fragments arranged by size:
1377 730 611 542 358 190 93 74
62
BspEI T'CCGG_A
Cuts at: 143 3257 143
Size: 3114 923
BspHI T'CATG_A
Cuts at: 3213 3213
Size: 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 615 749 1087 1301 1603 1984 615
Size: 134 338 214 302 381 2668
Fragments arranged by size:
2668 381 338 302 214 134
BsrBI CCG'CTC
Cuts at: 50 2329 2383 3867 4032 50
Size: 2279 54 1484 165 55
Fragments arranged by size:
2279 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1949 1949
Size: 4037
BsrFI r'CCGG_y
Cuts at: 185 2035 2216 2425 185
Size: 1850 181 209 1797
Fragments arranged by size:
1850 1797 209 181
BssSI C'ACGA_G
Cuts at: 2308 2666 2308
Size: 358 3679
BstAPI GCAn_nnn'nTGC
Cuts at: 1414 1414
Size: 4037
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstXI CCAn_nnnn'nTGG
Cuts at: 804 804
Size: 4037
BstYI r'GATC_y
Cuts at: 1 452 1062 1529 1552 1887 2133 3145 3231
Size: 451 610 467 23 335 246 1012 86
Cuts at: 3231 3243 3589 1
Size: 12 346 449
Fragments arranged by size:
1012 610 467 451 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 914 914
Size: 4037
BtgI C'CryG_G
Cuts at: 107 388 779 817 107
Size: 281 391 38 3327
Fragments arranged by size:
3327 391 281 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 486 1987 2168 2331 486
Size: 1501 181 163 2192
Fragments arranged by size:
2192 1501 181 163
BtsI GCAGTG_nn'
Cuts at: 849 1280 849
Size: 431 3606
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 812 847 812
Size: 35 4002
DraI TTT'AAA
Cuts at: 1036 1036
Size: 4037
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2601 1743
Size: 858 3179
EaeI y'GGCC_r
Cuts at: 158 183 308 820 1622 1796 2187 2214 3559
Size: 25 125 512 802 174 391 27 1345
Cuts at: 3559 158
Size: 636
Fragments arranged by size:
1345 802 636 512 391 174 125 27
25
EagI C'GGCC_G
Cuts at: 308 1622 308
Size: 1314 2723
EarI CTCTTCn'nnn_
Cuts at: 632 643 712 1027 2060 2270 632
Size: 11 69 315 1033 210 2399
Fragments arranged by size:
2399 1033 315 210 69 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2245 2555 2701 3847 2245
Size: 310 146 1146 2435
Fragments arranged by size:
2435 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1049 1049
Size: 4037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 397 1008 1862 2294 3041 3309 256
Size: 141 611 854 432 747 268 984
Fragments arranged by size:
984 854 747 611 432 268 141
EcoO109I rG'GnC_Cy
Cuts at: 689 768 918 1176 689
Size: 79 150 258 3550
Fragments arranged by size:
3550 258 150 79
EcoRI G'AATT_C
Cuts at: 1041 1041
Size: 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 4037
FspI TGC'GCA
Cuts at: 1818 1818
Size: 4037
HaeII r_GCGC'y
Cuts at: 139 791 1471 1719 2741 3720 139
Size: 652 680 248 1022 979 456
Fragments arranged by size:
1022 979 680 652 456 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 424 456 665 697 897 929 1879
Size: 32 103 32 209 32 200 32 950
Cuts at: 1879 1909 1911 1941 289
Size: 30 2 30 2385
Fragments arranged by size:
2385 950 209 200 103 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 978 1076 978
Size: 98 3939
KasI G'GCGC_C
Cuts at: 1467 1715 1467
Size: 248 3789
KpnI G_GTAC'C
Cuts at: 1057 1057
Size: 4037
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2708 2892 2708
Size: 184 3853
MscI TGG'CCA
Cuts at: 160 1798 160
Size: 1638 2399
MslI CAynn'nnrTG
Cuts at: 802 2026 2153 802
Size: 1224 127 2686
Fragments arranged by size:
2686 1224 127
MspA1I CmG'CkG
Cuts at: 774 819 1001 1148 1462 1822 2835 3080 774
Size: 45 182 147 314 360 1013 245 1731
Fragments arranged by size:
1731 1013 360 314 245 182 147 45
NaeI GCC'GGC
Cuts at: 187 2218 187
Size: 2031 2006
NarI GG'CG_CC
Cuts at: 1468 1716 1468
Size: 248 3789
NcoI C'CATG_G
Cuts at: 107 107
Size: 4037
NgoMIV G'CCGG_C
Cuts at: 185 2216 185
Size: 2031 2006
NsiI A_TGCA'T
Cuts at: 953 953
Size: 4037
NspI r_CATG'y
Cuts at: 951 1090 1590 2497 951
Size: 139 500 907 2491
Fragments arranged by size:
2491 907 500 139
PciI A'CATG_T
Cuts at: 2493 2493
Size: 4037
PfoI T'CCnGG_A
Cuts at: 920 920
Size: 4037
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4005
PpuMI rG'GwC_Cy
Cuts at: 918 918
Size: 4037
PspOMI G'GGCC_C
Cuts at: 689 689
Size: 4037
PspXI vC'TCGA_Gb
Cuts at: 529 529
Size: 4037
PstI C_TGCA'G
Cuts at: 365 1084 1769 365
Size: 719 685 2633
Fragments arranged by size:
2633 719 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 1462 1822 1462
Size: 360 3677
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 1051 1051
Size: 4037
SacII CC_GC'GG
Cuts at: 820 820
Size: 4037
SalI G'TCGA_C
Cuts at: 976 1074 976
Size: 98 3939
SapI GCTCTTCn'nnn_
Cuts at: 712 2060 2270 712
Size: 1348 210 2479
Fragments arranged by size:
2479 1348 210
SbfI CC_TGCA'GG
Cuts at: 1084 1084
Size: 4037
ScaI AGT'ACT
Cuts at: 3554 3554
Size: 4037
SfcI C'TryA_G
Cuts at: 33 335 361 1080 1765 2758 2949 33
Size: 302 26 719 685 993 191 1121
Fragments arranged by size:
1121 993 719 685 302 191 26
SfoI GGC'GCC
Cuts at: 1469 1717 1469
Size: 248 3789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SmaI CCC'GGG
Cuts at: 1059 1059
Size: 4037
SmlI C'TyrA_G
Cuts at: 118 256 529 831 1192 2107 2599 2861 118
Size: 138 273 302 361 915 492 262 1294
Fragments arranged by size:
1294 915 492 361 302 273 262 138
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 1090 1590 1090
Size: 500 3537
StuI AGG'CCT
Cuts at: 598 598
Size: 4037
StyI C'CwwG_G
Cuts at: 107 290 632 1171 1525 107
Size: 183 342 539 354 2619
Fragments arranged by size:
2619 539 354 342 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1620 1753 2212 2421 1620
Size: 133 459 209 3236
Fragments arranged by size:
3236 459 209 133
TatI w'GTAC_w
Cuts at: 3552 3552
Size: 4037
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4005
Tth111I GACn'n_nGTC
Cuts at: 1834 1834
Size: 4037
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 561 1532 561
Size: 971 3066
XhoI C'TCGA_G
Cuts at: 529 529
Size: 4037
XmaI C'CCGG_G
Cuts at: 1057 1057
Size: 4037
ZraI GAC'GTC
Cuts at: 681 681
Size: 4037
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BglI BmgBI BmtI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PshAI PsiI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI