Restriction map for
pF1KB6415
(Circular) (Six-base) MAPSORT of: pF1KB6415.seq Check: 6400 from: 1 to: 4037
pF1KB6415 4037 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 533 1075 533
Size: 542 3495
Acc65I G'GTAC_C
Cuts at: 1053 1053
Size: 4037
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 1862 2294 3041 3309 482
Size: 1380 432 747 268 1210
Fragments arranged by size:
1380 1210 747 432 268
AfeI AGC'GCT
Cuts at: 946 946
Size: 4037
AflIII A'CryG_T
Cuts at: 1389 2493 3470 1389
Size: 1104 977 1956
Fragments arranged by size:
1956 1104 977
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AhdI GACnn_n'nnGTC
Cuts at: 305 305
Size: 4037
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 644 676 644
Size: 32 4005
AlwNI CAG_nnn'CTG
Cuts at: 834 1342 2909 834
Size: 508 1567 1962
Fragments arranged by size:
1962 1567 508
ApaI G_GGCC'C
Cuts at: 199 199
Size: 4037
ApaLI G'TGCA_C
Cuts at: 674 2807 674
Size: 2133 1904
ApoI r'AATT_y
Cuts at: 1041 3390 3646 1041
Size: 2349 256 1432
Fragments arranged by size:
2349 1432 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 264 325 1057 264
Size: 61 732 3244
Fragments arranged by size:
3244 732 61
BamHI G'GATC_C
Cuts at: 1062 1062
Size: 4037
BanI G'GyrC_C
Cuts at: 268 402 907 1053 1467 1715 1750 268
Size: 134 505 146 414 248 35 2555
Fragments arranged by size:
2555 505 414 248 146 134 35
BanII G_rGCy'C
Cuts at: 199 1051 2081 199
Size: 852 1030 2155
Fragments arranged by size:
2155 1030 852
BbeI G_GCGC'C
Cuts at: 406 1471 1719 406
Size: 1065 248 2724
Fragments arranged by size:
2724 1065 248
BciVI GTATCCnnnnn_n'
Cuts at: 1935 2696 4037 1935
Size: 761 1341 1935
Fragments arranged by size:
1935 1341 761
BclI T'GATC_A
Cuts at: 1557 1557
Size: 4037
BglI GCCn_nnn'nGGC
Cuts at: 179 277 179
Size: 98 3939
BglII A'GATC_T
Cuts at: 1552 3589 1552
Size: 2037 2000
BlpI GC'TnA_GC
Cuts at: 346 1149 346
Size: 803 3234
Bme1580I G_kGCm'C
Cuts at: 199 273 482 678 698 1662 1755 2811 199
Size: 74 209 196 20 964 93 1056 1425
Fragments arranged by size:
1425 1056 964 209 196 93 74 20
BmrI ACTGGGnnnn_n'
Cuts at: 1249 1330 1665 3809 1249
Size: 81 335 2144 1477
Fragments arranged by size:
2144 1477 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 663 2404 663
Size: 1741 2296
Bpu10I CC'TnA_GC
Cuts at: 351 351
Size: 4037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 2092 2584 2882 1213
Size: 879 492 298 2368
Fragments arranged by size:
2368 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 750 3741 750
Size: 2991 1046
BsaAI yAC'GTr
Cuts at: 1390 1390
Size: 4037
BsaBI GATnn'nnATC
Cuts at: 6 1551 3588 6
Size: 1545 2037 455
Fragments arranged by size:
2037 1545 455
BsaHI Gr'CG_yC
Cuts at: 403 1468 1716 2418 3914 403
Size: 1065 248 702 1496 526
Fragments arranged by size:
1496 1065 702 526 248
BsaWI w'CCGG_w
Cuts at: 1226 1747 2425 2699 2846 3257 1226
Size: 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 135 165 135
Size: 30 4007
BseYI C'CCAG_C
Cuts at: 396 1462 2220 2797 3293 396
Size: 1066 758 577 496 1140
Fragments arranged by size:
1140 1066 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 196
Size: 4037
BsiEI CG_ry'CG
Cuts at: 104 284 290 1579 1625 2833 104
Size: 180 6 1289 46 1208 1308
Fragments arranged by size:
1308 1289 1208 180 46 6
BsiHKAI G_wGCw'C
Cuts at: 678 1051 1829 2019 2811 678
Size: 373 778 190 792 1904
Fragments arranged by size:
1904 792 778 373 190
Bsp1286I G_dGCh'C
Cuts at: 199 273 482 678 698 1051 1662 1755 1829
Size: 74 209 196 20 353 611 93 74
Cuts at: 1829 2019 2081 2811 199
Size: 190 62 730 1425
Fragments arranged by size:
1425 730 611 353 209 196 190 93
74 74 62 20
BspEI T'CCGG_A
Cuts at: 3257 3257
Size: 4037
BspHI T'CATG_A
Cuts at: 3213 3213
Size: 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 254 1087 1301 1603 1984 254
Size: 833 214 302 381 2307
Fragments arranged by size:
2307 833 381 302 214
BsrBI CCG'CTC
Cuts at: 50 136 2329 2383 3867 4032 50
Size: 86 2193 54 1484 165 55
Fragments arranged by size:
2193 1484 165 86 55 54
BsrDI GCAATG_nn'
Cuts at: 811 1949 811
Size: 1138 2899
BsrFI r'CCGG_y
Cuts at: 228 2035 2216 2425 228
Size: 1807 181 209 1840
Fragments arranged by size:
1840 1807 209 181
BssHII G'CGCG_C
Cuts at: 430 430
Size: 4037
BssSI C'ACGA_G
Cuts at: 706 737 2308 2666 706
Size: 31 1571 358 2077
Fragments arranged by size:
2077 1571 358 31
BstAPI GCAn_nnn'nTGC
Cuts at: 1414 1414
Size: 4037
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstXI CCAn_nnnn'nTGG
Cuts at: 504 504
Size: 4037
BstYI r'GATC_y
Cuts at: 1 1062 1529 1552 1887 2133 3145 3231 3243
Size: 1061 467 23 335 246 1012 86 12
Cuts at: 3243 3589 1
Size: 346 449
Fragments arranged by size:
1061 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 147 284 587 147
Size: 137 303 3597
Fragments arranged by size:
3597 303 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1987 2168 2331 1987
Size: 181 163 3693
Fragments arranged by size:
3693 181 163
BtsI GCAGTG_nn'
Cuts at: 576 1280 576
Size: 704 3333
DraI TTT'AAA
Cuts at: 1036 1036
Size: 4037
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2601 1743
Size: 858 3179
EaeI y'GGCC_r
Cuts at: 281 287 573 584 1622 1796 2187 2214 3559
Size: 6 286 11 1038 174 391 27 1345
Cuts at: 3559 281
Size: 759
Fragments arranged by size:
1345 1038 759 391 286 174 27 11
6
EagI C'GGCC_G
Cuts at: 281 287 1622 281
Size: 6 1335 2696
Fragments arranged by size:
2696 1335 6
EarI CTCTTCn'nnn_
Cuts at: 509 2060 2270 509
Size: 1551 210 2276
Fragments arranged by size:
2276 1551 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2245 2555 2701 3847 2245
Size: 310 146 1146 2435
Fragments arranged by size:
2435 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1049 1049
Size: 4037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 1862 2294 3041 3309 482
Size: 1380 432 747 268 1210
Fragments arranged by size:
1380 1210 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 195 196 299 307 1176 195
Size: 1 103 8 869 3056
Fragments arranged by size:
3056 869 103 8 1
EcoRI G'AATT_C
Cuts at: 1041 1041
Size: 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 651 966 1005 2056 651
Size: 315 39 1051 2632
Fragments arranged by size:
2632 1051 315 39
FspI TGC'GCA
Cuts at: 868 1818 868
Size: 950 3087
HaeII r_GCGC'y
Cuts at: 174 259 406 425 835 948 1471 1719 2741
Size: 85 147 19 410 113 523 248 1022
Cuts at: 2741 3720 174
Size: 979 491
Fragments arranged by size:
1022 979 523 491 410 248 147 113
85 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 291 323 1879 1909 1911 1941 291
Size: 32 1556 30 2 30 2387
Fragments arranged by size:
2387 1556 32 30 30 2
HincII GTy'rAC
Cuts at: 1076 1076
Size: 4037
KasI G'GCGC_C
Cuts at: 402 1467 1715 402
Size: 1065 248 2724
Fragments arranged by size:
2724 1065 248
KpnI G_GTAC'C
Cuts at: 1057 1057
Size: 4037
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2708 2892 2708
Size: 184 3853
MscI TGG'CCA
Cuts at: 586 1798 586
Size: 1212 2825
MslI CAynn'nnrTG
Cuts at: 229 485 502 586 706 821 2026 2153 229
Size: 256 17 84 120 115 1205 127 2113
Fragments arranged by size:
2113 1205 256 127 120 115 84 17
MspA1I CmG'CkG
Cuts at: 131 149 210 286 563 666 808 1148 1462
Size: 18 61 76 277 103 142 340 314
Cuts at: 1462 1822 2835 3080 131
Size: 360 1013 245 1088
Fragments arranged by size:
1088 1013 360 340 314 277 245 142
103 76 61 18
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 4037
NarI GG'CG_CC
Cuts at: 403 1468 1716 403
Size: 1065 248 2724
Fragments arranged by size:
2724 1065 248
NcoI C'CATG_G
Cuts at: 587 587
Size: 4037
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 4037
NotI GC'GGCC_GC
Cuts at: 281 281
Size: 4037
NspI r_CATG'y
Cuts at: 228 1090 1590 2497 228
Size: 862 500 907 1768
Fragments arranged by size:
1768 907 862 500
PasI CC'CwG_GG
Cuts at: 191 303 191
Size: 112 3925
PciI A'CATG_T
Cuts at: 2493 2493
Size: 4037
PflMI CCAn_nnn'nTGG
Cuts at: 582 582
Size: 4037
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4005
PpuMI rG'GwC_Cy
Cuts at: 299 307 299
Size: 8 4029
PspOMI G'GGCC_C
Cuts at: 195 195
Size: 4037
PstI C_TGCA'G
Cuts at: 251 1084 1769 251
Size: 833 685 2519
Fragments arranged by size:
2519 833 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 666 1462 1822 666
Size: 796 360 2881
Fragments arranged by size:
2881 796 360
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 1051 1051
Size: 4037
SacII CC_GC'GG
Cuts at: 150 287 150
Size: 137 3900
SalI G'TCGA_C
Cuts at: 1074 1074
Size: 4037
SanDI GG'GwC_CC
Cuts at: 299 307 299
Size: 8 4029
SapI GCTCTTCn'nnn_
Cuts at: 2060 2270 2060
Size: 210 3827
SbfI CC_TGCA'GG
Cuts at: 251 1084 251
Size: 833 3204
ScaI AGT'ACT
Cuts at: 3554 3554
Size: 4037
SexAI A'CCwGG_T
Cuts at: 725 725
Size: 4037
SfcI C'TryA_G
Cuts at: 33 247 1080 1765 2758 2949 33
Size: 214 833 685 993 191 1121
Fragments arranged by size:
1121 993 833 685 214 191
SfoI GGC'GCC
Cuts at: 404 1469 1717 404
Size: 1065 248 2724
Fragments arranged by size:
2724 1065 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SmaI CCC'GGG
Cuts at: 266 1059 266
Size: 793 3244
SmlI C'TyrA_G
Cuts at: 1192 2107 2599 2861 1192
Size: 915 492 262 2368
Fragments arranged by size:
2368 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 228 1090 1590 228
Size: 862 500 2675
Fragments arranged by size:
2675 862 500
StuI AGG'CCT
Cuts at: 646 646
Size: 4037
StyI C'CwwG_G
Cuts at: 587 927 1171 1525 587
Size: 340 244 354 3099
Fragments arranged by size:
3099 354 340 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1620 1753 2212 2421 1620
Size: 133 459 209 3236
Fragments arranged by size:
3236 459 209 133
TatI w'GTAC_w
Cuts at: 611 3552 611
Size: 2941 1096
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 135 167 3554 3586 135
Size: 32 3387 32 586
Fragments arranged by size:
3387 586 32 32
Tth111I GACn'n_nGTC
Cuts at: 1834 1834
Size: 4037
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 705 1532 705
Size: 827 3210
XmaI C'CCGG_G
Cuts at: 264 1057 264
Size: 793 3244
XmnI GAAnn'nnTTC
Cuts at: 946 946
Size: 4037
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI BpmI BseRI
BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI