Restriction map for
pF1KB6418
(Circular) (Six-base) MAPSORT of: pF1KB6418.seq Check: 8374 from: 1 to: 4124
pF1KB6418 4124 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 428 1162 428
Size: 734 3390
Acc65I G'GTAC_C
Cuts at: 1140 1140
Size: 4124
AclI AA'CG_TT
Cuts at: 3964 3964
Size: 4124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1949 2381 3128 3396 1949
Size: 432 747 268 2677
Fragments arranged by size:
2677 747 432 268
AflIII A'CryG_T
Cuts at: 1476 2580 3557 1476
Size: 1104 977 2043
Fragments arranged by size:
2043 1104 977
AgeI A'CCGG_T
Cuts at: 2512 2512
Size: 4124
AleI CACnn'nnGTG
Cuts at: 175 178 175
Size: 3 4121
AlwNI CAG_nnn'CTG
Cuts at: 288 1429 2996 288
Size: 1141 1567 1416
Fragments arranged by size:
1567 1416 1141
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 521 554 1128 3477 3733 521
Size: 33 574 2349 256 912
Fragments arranged by size:
2349 912 574 256 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 574 1144 574
Size: 570 3554
BamHI G'GATC_C
Cuts at: 1040 1149 1040
Size: 109 4015
BanI G'GyrC_C
Cuts at: 811 918 1140 1554 1802 1837 811
Size: 107 222 414 248 35 3098
Fragments arranged by size:
3098 414 248 222 107 35
BanII G_rGCy'C
Cuts at: 118 491 575 731 1138 2168 118
Size: 373 84 156 407 1030 2074
Fragments arranged by size:
2074 1030 407 373 156 84
BbeI G_GCGC'C
Cuts at: 815 1558 1806 815
Size: 743 248 3133
Fragments arranged by size:
3133 743 248
BbsI GAAGACnn'nnnn_
Cuts at: 743 743
Size: 4124
BciVI GTATCCnnnnn_n'
Cuts at: 426 2022 2783 4124 426
Size: 1596 761 1341 426
Fragments arranged by size:
1596 1341 761 426
BclI T'GATC_A
Cuts at: 1644 1644
Size: 4124
BglI GCCn_nnn'nGGC
Cuts at: 940 940
Size: 4124
BglII A'GATC_T
Cuts at: 1639 3676 1639
Size: 2037 2087
BlpI GC'TnA_GC
Cuts at: 1236 1236
Size: 4124
Bme1580I G_kGCm'C
Cuts at: 1749 1842 2898 1749
Size: 93 1056 2975
Fragments arranged by size:
2975 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1336 1417 1752 3896 1336
Size: 81 335 2144 1564
Fragments arranged by size:
2144 1564 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1047 2491 1047
Size: 1444 2680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 1093
Size: 4124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 1300 2179 2671 2969 145
Size: 1155 879 492 298 1300
Fragments arranged by size:
1300 1155 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3828 3828
Size: 4124
BsaAI yAC'GTr
Cuts at: 1477 1477
Size: 4124
BsaBI GATnn'nnATC
Cuts at: 6 1638 3675 6
Size: 1632 2037 455
Fragments arranged by size:
2037 1632 455
BsaHI Gr'CG_yC
Cuts at: 812 1555 1803 2505 4001 812
Size: 743 248 702 1496 935
Fragments arranged by size:
1496 935 743 702 248
BsaWI w'CCGG_w
Cuts at: 1313 1834 2512 2786 2933 3344 1313
Size: 521 678 274 147 411 2093
Fragments arranged by size:
2093 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 866 896 866
Size: 30 4094
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 592 310
Size: 282 3842
BseYI C'CCAG_C
Cuts at: 321 502 1549 2307 2884 3380 321
Size: 181 1047 758 577 496 1065
Fragments arranged by size:
1065 1047 758 577 496 181
BsiEI CG_ry'CG
Cuts at: 104 1666 1712 2920 104
Size: 1562 46 1208 1308
Fragments arranged by size:
1562 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 118 491 575 929 1138 1916 2106 2898 118
Size: 373 84 354 209 778 190 792 1344
Fragments arranged by size:
1344 792 778 373 354 209 190 84
Bsp1286I G_dGCh'C
Cuts at: 118 491 575 731 929 1138 1749 1842 1916
Size: 373 84 156 198 209 611 93 74
Cuts at: 1916 2106 2168 2898 118
Size: 190 62 730 1344
Fragments arranged by size:
1344 730 611 373 209 198 190 156
93 84 74 62
BspEI T'CCGG_A
Cuts at: 3344 3344
Size: 4124
BspHI T'CATG_A
Cuts at: 3300 3300
Size: 4124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1174 1388 1690 2071 1174
Size: 214 302 381 3227
Fragments arranged by size:
3227 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1077 2416 2470 3954 4119 50
Size: 1027 1339 54 1484 165 55
Fragments arranged by size:
1484 1339 1027 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2036 2036
Size: 4124
BsrFI r'CCGG_y
Cuts at: 850 2122 2303 2512 850
Size: 1272 181 209 2462
Fragments arranged by size:
2462 1272 209 181
BssSI C'ACGA_G
Cuts at: 2395 2753 2395
Size: 358 3766
BstAPI GCAn_nnn'nTGC
Cuts at: 596 1501 596
Size: 905 3219
BstBI TT'CG_AA
Cuts at: 2485 2485
Size: 4124
BstXI CCAn_nnnn'nTGG
Cuts at: 180 807 885 957 180
Size: 627 78 72 3347
Fragments arranged by size:
3347 627 78 72
BstYI r'GATC_y
Cuts at: 1 1040 1149 1616 1639 1974 2220 3232 3318
Size: 1039 109 467 23 335 246 1012 86
Cuts at: 3318 3330 3676 1
Size: 12 346 449
Fragments arranged by size:
1039 1012 467 449 346 335 246 109
86 23 12
BtgI C'CryG_G
Cuts at: 176 176
Size: 4124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 472 2074 2255 2418 472
Size: 1602 181 163 2178
Fragments arranged by size:
2178 1602 181 163
BtsI GCAGTG_nn'
Cuts at: 594 1070 1367 594
Size: 476 297 3351
Fragments arranged by size:
3351 476 297
DraI TTT'AAA
Cuts at: 1123 1123
Size: 4124
DrdI GACnn_nn'nnGTC
Cuts at: 1830 2688 1830
Size: 858 3266
EaeI y'GGCC_r
Cuts at: 182 257 312 464 848 1709 1883 2274 2301
Size: 75 55 152 384 861 174 391 27
Cuts at: 2301 3646 182
Size: 1345 660
Fragments arranged by size:
1345 861 660 391 384 174 152 75
55 27
EagI C'GGCC_G
Cuts at: 1709 1709
Size: 4124
EarI CTCTTCn'nnn_
Cuts at: 619 2147 2357 619
Size: 1528 210 2386
Fragments arranged by size:
2386 1528 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2332 2642 2788 3934 2332
Size: 310 146 1146 2522
Fragments arranged by size:
2522 1146 310 146
EcoICRI GAG'CTC
Cuts at: 116 489 573 1136 116
Size: 373 84 563 3104
Fragments arranged by size:
3104 563 373 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 1949 2381 3128 3396 1093
Size: 856 432 747 268 1821
Fragments arranged by size:
1821 856 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1263 1263
Size: 4124
EcoRI G'AATT_C
Cuts at: 521 554 1128 521
Size: 33 574 3517
Fragments arranged by size:
3517 574 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 4124
FspI TGC'GCA
Cuts at: 1905 1905
Size: 4124
HaeII r_GCGC'y
Cuts at: 815 1558 1806 2828 3807 815
Size: 743 248 1022 979 1132
Fragments arranged by size:
1132 1022 979 743 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 703 735 967 999 1966 1996 1998 2028 703
Size: 32 232 32 967 30 2 30 2799
Fragments arranged by size:
2799 967 232 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1163 1163
Size: 4124
KasI G'GCGC_C
Cuts at: 811 1554 1802 811
Size: 743 248 3133
Fragments arranged by size:
3133 743 248
KpnI G_GTAC'C
Cuts at: 1144 1144
Size: 4124
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2795 2979 2795
Size: 184 3940
MscI TGG'CCA
Cuts at: 466 1885 466
Size: 1419 2705
MslI CAynn'nnrTG
Cuts at: 175 178 805 974 2113 2240 175
Size: 3 627 169 1139 127 2059
Fragments arranged by size:
2059 1139 627 169 127 3
MspA1I CmG'CkG
Cuts at: 1057 1235 1549 1909 2922 3167 1057
Size: 178 314 360 1013 245 2014
Fragments arranged by size:
2014 1013 360 314 245 178
NaeI GCC'GGC
Cuts at: 852 2305 852
Size: 1453 2671
NarI GG'CG_CC
Cuts at: 812 1555 1803 812
Size: 743 248 3133
Fragments arranged by size:
3133 743 248
NgoMIV G'CCGG_C
Cuts at: 850 2303 850
Size: 1453 2671
NspI r_CATG'y
Cuts at: 700 1177 1677 2584 700
Size: 477 500 907 2240
Fragments arranged by size:
2240 907 500 477
PasI CC'CwG_GG
Cuts at: 742 742
Size: 4124
PciI A'CATG_T
Cuts at: 2580 2580
Size: 4124
PflMI CCAn_nnn'nTGG
Cuts at: 288 633 676 288
Size: 345 43 3736
Fragments arranged by size:
3736 345 43
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 4092
PsiI TTA'TAA
Cuts at: 529 529
Size: 4124
PspXI vC'TCGA_Gb
Cuts at: 574 574
Size: 4124
PstI C_TGCA'G
Cuts at: 902 1171 1856 902
Size: 269 685 3170
Fragments arranged by size:
3170 685 269
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 1549 1909 1549
Size: 360 3764
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 118 491 575 1138 118
Size: 373 84 563 3104
Fragments arranged by size:
3104 563 373 84
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 3641 3641
Size: 4124
SfcI C'TryA_G
Cuts at: 33 898 1167 1852 2845 3036 33
Size: 865 269 685 993 191 1121
Fragments arranged by size:
1121 993 865 685 269 191
SfoI GGC'GCC
Cuts at: 813 1556 1804 813
Size: 743 248 3133
Fragments arranged by size:
3133 743 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 1146 1146
Size: 4124
SmlI C'TyrA_G
Cuts at: 160 574 1279 2194 2686 2948 160
Size: 414 705 915 492 262 1336
Fragments arranged by size:
1336 915 705 492 414 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4124
SphI G_CATG'C
Cuts at: 1177 1677 1177
Size: 500 3624
StyI C'CwwG_G
Cuts at: 935 1258 1612 935
Size: 323 354 3447
Fragments arranged by size:
3447 354 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 533 891 1707 1840 2299 2508 533
Size: 358 816 133 459 209 2149
Fragments arranged by size:
2149 816 459 358 209 133
TatI w'GTAC_w
Cuts at: 215 3639 215
Size: 3424 700
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 866 898 3641 3673 866
Size: 32 2743 32 1317
Fragments arranged by size:
2743 1317 32 32
Tth111I GACn'n_nGTC
Cuts at: 1921 1921
Size: 4124
XbaI T'CTAG_A
Cuts at: 1155 1155
Size: 4124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1619 1619
Size: 4124
XhoI C'TCGA_G
Cuts at: 574 574
Size: 4124
XmaI C'CCGG_G
Cuts at: 1144 1144
Size: 4124
XmnI GAAnn'nnTTC
Cuts at: 554 985 554
Size: 431 3693
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PsiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PpuMI PshAI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI