(Circular) (Six-base) MAPSORT of: pF1KB6419.seq Check: 2010 from: 1 to: 4040 pF1KB6419 4040 bp With 182 enzymes: SgfI * June 26, 2008 10:46 .. AccI GT'mk_AC Cuts at: 1078 1078 Size: 4040 Acc65I G'GTAC_C Cuts at: 1056 1056 Size: 4040 AclI AA'CG_TT Cuts at: 3880 3880 Size: 4040 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 264 1865 2297 3044 3312 264 Size: 1601 432 747 268 992 Fragments arranged by size: 1601 992 747 432 268 AflIII A'CryG_T Cuts at: 882 1392 2496 3473 882 Size: 510 1104 977 1449 Fragments arranged by size: 1449 1104 977 510 AgeI A'CCGG_T Cuts at: 2428 2428 Size: 4040 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 869 901 869 Size: 32 4008 AlwNI CAG_nnn'CTG Cuts at: 858 1345 2912 858 Size: 487 1567 1986 Fragments arranged by size: 1986 1567 487 ApaLI G'TGCA_C Cuts at: 2810 2810 Size: 4040 ApoI r'AATT_y Cuts at: 1044 3393 3649 1044 Size: 2349 256 1435 Fragments arranged by size: 2349 1435 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4040 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4040 AvaI C'yCGr_G Cuts at: 530 1060 530 Size: 530 3510 BamHI G'GATC_C Cuts at: 1065 1065 Size: 4040 BanI G'GyrC_C Cuts at: 813 1056 1470 1718 1753 813 Size: 243 414 248 35 3100 Fragments arranged by size: 3100 414 248 243 35 BanII G_rGCy'C Cuts at: 1054 2084 1054 Size: 1030 3010 BbeI G_GCGC'C Cuts at: 1474 1722 1474 Size: 248 3792 BbsI GAAGACnn'nnnn_ Cuts at: 915 915 Size: 4040 BciVI GTATCCnnnnn_n' Cuts at: 1938 2699 4040 1938 Size: 761 1341 1938 Fragments arranged by size: 1938 1341 761 BclI T'GATC_A Cuts at: 1560 1560 Size: 4040 BfrBI ATG'CAT Cuts at: 1006 1006 Size: 4040 BglII A'GATC_T Cuts at: 718 1555 3592 718 Size: 837 2037 1166 Fragments arranged by size: 2037 1166 837 BlpI GC'TnA_GC Cuts at: 704 1152 704 Size: 448 3592 Bme1580I G_kGCm'C Cuts at: 1665 1758 2814 1665 Size: 93 1056 2891 Fragments arranged by size: 2891 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1025 1252 1333 1668 3812 1025 Size: 227 81 335 2144 1253 Fragments arranged by size: 2144 1253 335 227 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2407 2407 Size: 4040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 688 980 688 Size: 292 3748 Bpu10I CC'TnA_GC Cuts at: 890 890 Size: 4040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 763 916 1216 2095 2587 2885 763 Size: 153 300 879 492 298 1918 Fragments arranged by size: 1918 879 492 300 298 153 BsaI GGTCTCn'nnnn_ Cuts at: 3744 3744 Size: 4040 BsaAI yAC'GTr Cuts at: 1393 1393 Size: 4040 BsaBI GATnn'nnATC Cuts at: 6 1554 3591 6 Size: 1548 2037 455 Fragments arranged by size: 2037 1548 455 BsaHI Gr'CG_yC Cuts at: 1471 1719 2421 3917 1471 Size: 248 702 1496 1594 Fragments arranged by size: 1594 1496 702 248 BsaWI w'CCGG_w Cuts at: 1229 1750 2428 2702 2849 3260 1229 Size: 521 678 274 147 411 2009 Fragments arranged by size: 2009 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 245 245 Size: 4040 BseYI C'CCAG_C Cuts at: 1465 2223 2800 3296 1465 Size: 758 577 496 2209 Fragments arranged by size: 2209 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1582 1628 2836 104 Size: 1478 46 1208 1308 Fragments arranged by size: 1478 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 996 1054 1832 2022 2814 996 Size: 58 778 190 792 2222 Fragments arranged by size: 2222 792 778 190 58 BsmI GAATG_Cn' Cuts at: 1006 1006 Size: 4040 Bsp1286I G_dGCh'C Cuts at: 996 1054 1665 1758 1832 2022 2084 2814 996 Size: 58 611 93 74 190 62 730 2222 Fragments arranged by size: 2222 730 611 190 93 74 62 58 BspEI T'CCGG_A Cuts at: 3260 3260 Size: 4040 BspHI T'CATG_A Cuts at: 3216 3216 Size: 4040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1090 1304 1606 1987 1090 Size: 214 302 381 3143 Fragments arranged by size: 3143 381 302 214 BsrBI CCG'CTC Cuts at: 50 2332 2386 3870 4035 50 Size: 2282 54 1484 165 55 Fragments arranged by size: 2282 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1952 1952 Size: 4040 BsrFI r'CCGG_y Cuts at: 2038 2219 2428 2038 Size: 181 209 3650 Fragments arranged by size: 3650 209 181 BssSI C'ACGA_G Cuts at: 2311 2669 2311 Size: 358 3682 BstAPI GCAn_nnn'nTGC Cuts at: 1417 1417 Size: 4040 BstBI TT'CG_AA Cuts at: 2401 2401 Size: 4040 BstXI CCAn_nnnn'nTGG Cuts at: 444 444 Size: 4040 BstYI r'GATC_y Cuts at: 1 300 718 1065 1532 1555 1890 2136 3148 Size: 299 418 347 467 23 335 246 1012 Cuts at: 3148 3234 3246 3592 1 Size: 86 12 346 449 Fragments arranged by size: 1012 467 449 418 347 346 335 299 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4040 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1990 2171 2334 1990 Size: 181 163 3696 Fragments arranged by size: 3696 181 163 BtsI GCAGTG_nn' Cuts at: 856 1283 856 Size: 427 3613 DraI TTT'AAA Cuts at: 923 1039 923 Size: 116 3924 DrdI GACnn_nn'nnGTC Cuts at: 1746 2604 1746 Size: 858 3182 EaeI y'GGCC_r Cuts at: 1625 1799 2190 2217 3562 1625 Size: 174 391 27 1345 2103 Fragments arranged by size: 2103 1345 391 174 27 EagI C'GGCC_G Cuts at: 1625 1625 Size: 4040 EarI CTCTTCn'nnn_ Cuts at: 2063 2273 2063 Size: 210 3830 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2248 2558 2704 3850 2248 Size: 310 146 1146 2438 Fragments arranged by size: 2438 1146 310 146 EcoICRI GAG'CTC Cuts at: 1052 1052 Size: 4040 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 264 688 980 1865 2297 3044 3312 264 Size: 424 292 885 432 747 268 992 Fragments arranged by size: 992 885 747 432 424 292 268 EcoO109I rG'GnC_Cy Cuts at: 951 1179 951 Size: 228 3812 EcoRI G'AATT_C Cuts at: 1044 1044 Size: 4040 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 294 2059 294 Size: 1765 2275 FspI TGC'GCA Cuts at: 1821 1821 Size: 4040 HaeII r_GCGC'y Cuts at: 1474 1722 2744 3723 1474 Size: 248 1022 979 1791 Fragments arranged by size: 1791 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 179 211 663 695 1882 1912 1914 1944 179 Size: 32 452 32 1187 30 2 30 2275 Fragments arranged by size: 2275 1187 452 32 32 30 30 2 HincII GTy'rAC Cuts at: 312 799 1079 312 Size: 487 280 3273 Fragments arranged by size: 3273 487 280 HindIII A'AGCT_T Cuts at: 554 826 554 Size: 272 3768 KasI G'GCGC_C Cuts at: 1470 1718 1470 Size: 248 3792 KpnI G_GTAC'C Cuts at: 1060 1060 Size: 4040 MluI A'CGCG_T Cuts at: 3473 3473 Size: 4040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2711 2895 2711 Size: 184 3856 MscI TGG'CCA Cuts at: 1801 1801 Size: 4040 MslI CAynn'nnrTG Cuts at: 790 2029 2156 790 Size: 1239 127 2674 Fragments arranged by size: 2674 1239 127 MspA1I CmG'CkG Cuts at: 1151 1465 1825 2838 3083 1151 Size: 314 360 1013 245 2108 Fragments arranged by size: 2108 1013 360 314 245 NaeI GCC'GGC Cuts at: 2221 2221 Size: 4040 NarI GG'CG_CC Cuts at: 1471 1719 1471 Size: 248 3792 NcoI C'CATG_G Cuts at: 107 107 Size: 4040 NdeI CA'TA_TG Cuts at: 873 873 Size: 4040 NgoMIV G'CCGG_C Cuts at: 2219 2219 Size: 4040 NsiI A_TGCA'T Cuts at: 1008 1008 Size: 4040 NspI r_CATG'y Cuts at: 886 1093 1593 2500 886 Size: 207 500 907 2426 Fragments arranged by size: 2426 907 500 207 PciI A'CATG_T Cuts at: 882 2496 882 Size: 1614 2426 PmeI GTTT'AAAC Cuts at: 1039 1039 Size: 4040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3179 3211 3179 Size: 32 4008 PpuMI rG'GwC_Cy Cuts at: 951 951 Size: 4040 PstI C_TGCA'G Cuts at: 623 863 1087 1772 623 Size: 240 224 685 2891 Fragments arranged by size: 2891 685 240 224 PvuI CG_AT'CG Cuts at: 104 104 Size: 4040 PvuII CAG'CTG Cuts at: 1465 1825 1465 Size: 360 3680 RsrII CG'GwC_CG Cuts at: 2235 2235 Size: 4040 SacI G_AGCT'C Cuts at: 1054 1054 Size: 4040 SalI G'TCGA_C Cuts at: 1077 1077 Size: 4040 SapI GCTCTTCn'nnn_ Cuts at: 2063 2273 2063 Size: 210 3830 SbfI CC_TGCA'GG Cuts at: 1087 1087 Size: 4040 ScaI AGT'ACT Cuts at: 3557 3557 Size: 4040 SfcI C'TryA_G Cuts at: 33 619 859 997 1083 1768 2761 2952 33 Size: 586 240 138 86 685 993 191 1121 Fragments arranged by size: 1121 993 685 586 240 191 138 86 SfoI GGC'GCC Cuts at: 1472 1720 1472 Size: 248 3792 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4040 SmaI CCC'GGG Cuts at: 1062 1062 Size: 4040 SmlI C'TyrA_G Cuts at: 530 742 895 1195 2110 2602 2864 530 Size: 212 153 300 915 492 262 1706 Fragments arranged by size: 1706 915 492 300 262 212 153 SpeI A'CTAG_T Cuts at: 67 67 Size: 4040 SphI G_CATG'C Cuts at: 1093 1593 1093 Size: 500 3540 StyI C'CwwG_G Cuts at: 107 1174 1528 107 Size: 1067 354 2619 Fragments arranged by size: 2619 1067 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1623 1756 2215 2424 1623 Size: 133 459 209 3239 Fragments arranged by size: 3239 459 209 133 TatI w'GTAC_w Cuts at: 3555 3555 Size: 4040 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3557 3589 3557 Size: 32 4008 Tth111I GACn'n_nGTC Cuts at: 1837 1837 Size: 4040 XbaI T'CTAG_A Cuts at: 1071 1071 Size: 4040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 468 1535 468 Size: 1067 2973 XhoI C'TCGA_G Cuts at: 530 530 Size: 4040 XmaI C'CCGG_G Cuts at: 1060 1060 Size: 4040 XmnI GAAnn'nnTTC Cuts at: 371 371 Size: 4040 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NheI NotI NruI PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI