Restriction map for
pF1KB6419
(Circular) (Six-base) MAPSORT of: pF1KB6419.seq Check: 2010 from: 1 to: 4040
pF1KB6419 4040 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 1865 2297 3044 3312 264
Size: 1601 432 747 268 992
Fragments arranged by size:
1601 992 747 432 268
AflIII A'CryG_T
Cuts at: 882 1392 2496 3473 882
Size: 510 1104 977 1449
Fragments arranged by size:
1449 1104 977 510
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 869 901 869
Size: 32 4008
AlwNI CAG_nnn'CTG
Cuts at: 858 1345 2912 858
Size: 487 1567 1986
Fragments arranged by size:
1986 1567 487
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 1044 3393 3649 1044
Size: 2349 256 1435
Fragments arranged by size:
2349 1435 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 530 1060 530
Size: 530 3510
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 813 1056 1470 1718 1753 813
Size: 243 414 248 35 3100
Fragments arranged by size:
3100 414 248 243 35
BanII G_rGCy'C
Cuts at: 1054 2084 1054
Size: 1030 3010
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbsI GAAGACnn'nnnn_
Cuts at: 915 915
Size: 4040
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BfrBI ATG'CAT
Cuts at: 1006 1006
Size: 4040
BglII A'GATC_T
Cuts at: 718 1555 3592 718
Size: 837 2037 1166
Fragments arranged by size:
2037 1166 837
BlpI GC'TnA_GC
Cuts at: 704 1152 704
Size: 448 3592
Bme1580I G_kGCm'C
Cuts at: 1665 1758 2814 1665
Size: 93 1056 2891
Fragments arranged by size:
2891 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1025 1252 1333 1668 3812 1025
Size: 227 81 335 2144 1253
Fragments arranged by size:
2144 1253 335 227 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 980 688
Size: 292 3748
Bpu10I CC'TnA_GC
Cuts at: 890 890
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 916 1216 2095 2587 2885 763
Size: 153 300 879 492 298 1918
Fragments arranged by size:
1918 879 492 300 298 153
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 245 245
Size: 4040
BseYI C'CCAG_C
Cuts at: 1465 2223 2800 3296 1465
Size: 758 577 496 2209
Fragments arranged by size:
2209 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 996 1054 1832 2022 2814 996
Size: 58 778 190 792 2222
Fragments arranged by size:
2222 792 778 190 58
BsmI GAATG_Cn'
Cuts at: 1006 1006
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 996 1054 1665 1758 1832 2022 2084 2814 996
Size: 58 611 93 74 190 62 730 2222
Fragments arranged by size:
2222 730 611 190 93 74 62 58
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 3216 3216
Size: 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1090 1304 1606 1987 1090
Size: 214 302 381 3143
Fragments arranged by size:
3143 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstXI CCAn_nnnn'nTGG
Cuts at: 444 444
Size: 4040
BstYI r'GATC_y
Cuts at: 1 300 718 1065 1532 1555 1890 2136 3148
Size: 299 418 347 467 23 335 246 1012
Cuts at: 3148 3234 3246 3592 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 418 347 346 335 299
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4040
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 856 1283 856
Size: 427 3613
DraI TTT'AAA
Cuts at: 923 1039 923
Size: 116 3924
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 1625 1799 2190 2217 3562 1625
Size: 174 391 27 1345 2103
Fragments arranged by size:
2103 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 688 980 1865 2297 3044 3312 264
Size: 424 292 885 432 747 268 992
Fragments arranged by size:
992 885 747 432 424 292 268
EcoO109I rG'GnC_Cy
Cuts at: 951 1179 951
Size: 228 3812
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 294 2059 294
Size: 1765 2275
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2744 3723 1474
Size: 248 1022 979 1791
Fragments arranged by size:
1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 179 211 663 695 1882 1912 1914 1944 179
Size: 32 452 32 1187 30 2 30 2275
Fragments arranged by size:
2275 1187 452 32 32 30 30 2
HincII GTy'rAC
Cuts at: 312 799 1079 312
Size: 487 280 3273
Fragments arranged by size:
3273 487 280
HindIII A'AGCT_T
Cuts at: 554 826 554
Size: 272 3768
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 790 2029 2156 790
Size: 1239 127 2674
Fragments arranged by size:
2674 1239 127
MspA1I CmG'CkG
Cuts at: 1151 1465 1825 2838 3083 1151
Size: 314 360 1013 245 2108
Fragments arranged by size:
2108 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NcoI C'CATG_G
Cuts at: 107 107
Size: 4040
NdeI CA'TA_TG
Cuts at: 873 873
Size: 4040
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NsiI A_TGCA'T
Cuts at: 1008 1008
Size: 4040
NspI r_CATG'y
Cuts at: 886 1093 1593 2500 886
Size: 207 500 907 2426
Fragments arranged by size:
2426 907 500 207
PciI A'CATG_T
Cuts at: 882 2496 882
Size: 1614 2426
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PpuMI rG'GwC_Cy
Cuts at: 951 951
Size: 4040
PstI C_TGCA'G
Cuts at: 623 863 1087 1772 623
Size: 240 224 685 2891
Fragments arranged by size:
2891 685 240 224
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 1465 1825 1465
Size: 360 3680
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 619 859 997 1083 1768 2761 2952 33
Size: 586 240 138 86 685 993 191 1121
Fragments arranged by size:
1121 993 685 586 240 191 138 86
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 530 742 895 1195 2110 2602 2864 530
Size: 212 153 300 915 492 262 1706
Fragments arranged by size:
1706 915 492 300 262 212 153
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StyI C'CwwG_G
Cuts at: 107 1174 1528 107
Size: 1067 354 2619
Fragments arranged by size:
2619 1067 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 468 1535 468
Size: 1067 2973
XhoI C'TCGA_G
Cuts at: 530 530
Size: 4040
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
XmnI GAAnn'nnTTC
Cuts at: 371 371
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI