Restriction map for
pF1KB6421
(Circular) (Six-base) MAPSORT of: pF1KB6421.seq Check: 5495 from: 1 to: 4040
pF1KB6421 4040 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 986 1865 2297 3044 3312 986
Size: 879 432 747 268 1714
Fragments arranged by size:
1714 879 747 432 268
AflII C'TTAA_G
Cuts at: 643 643
Size: 4040
AflIII A'CryG_T
Cuts at: 754 1392 2496 3473 754
Size: 638 1104 977 1321
Fragments arranged by size:
1321 1104 977 638
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 202 383 202
Size: 181 3859
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 368 400 368
Size: 32 4008
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 417 535 703 1007 1044 3393 3649 417
Size: 118 168 304 37 2349 256 808
Fragments arranged by size:
2349 808 304 256 168 118 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 232 269 1060 232
Size: 37 791 3212
Fragments arranged by size:
3212 791 37
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 138 1056 1470 1718 1753 138
Size: 918 414 248 35 2425
Fragments arranged by size:
2425 918 414 248 35
BanII G_rGCy'C
Cuts at: 1054 2084 1054
Size: 1030 3010
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbsI GAAGACnn'nnnn_
Cuts at: 508 508
Size: 4040
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 203 237 203
Size: 34 4006
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 642 1665 1758 2814 642
Size: 1023 93 1056 1868
Fragments arranged by size:
1868 1056 1023 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
Bpu10I CC'TnA_GC
Cuts at: 278 473 278
Size: 195 3845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 250
Size: 4040
BseYI C'CCAG_C
Cuts at: 161 1465 2223 2800 3296 161
Size: 1304 758 577 496 905
Fragments arranged by size:
1304 905 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 223 1582 1628 2836 104
Size: 119 1359 46 1208 1308
Fragments arranged by size:
1359 1308 1208 119 46
BsiHKAI G_wGCw'C
Cuts at: 821 1054 1832 2022 2814 821
Size: 233 778 190 792 2047
Fragments arranged by size:
2047 792 778 233 190
Bsp1286I G_dGCh'C
Cuts at: 642 821 1054 1665 1758 1832 2022 2084 2814
Size: 179 233 611 93 74 190 62 730
Cuts at: 2814 642
Size: 1868
Fragments arranged by size:
1868 730 611 233 190 179 93 74
62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 3216 3216
Size: 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1090 1304 1606 1987 1090
Size: 214 302 381 3143
Fragments arranged by size:
3143 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 460 1417 460
Size: 957 3083
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstYI r'GATC_y
Cuts at: 1 856 969 1065 1532 1555 1890 2136 3148
Size: 855 113 96 467 23 335 246 1012
Cuts at: 3148 3234 3246 3592 1
Size: 86 12 346 449
Fragments arranged by size:
1012 855 467 449 346 335 246 113
96 86 23 12
BtgI C'CryG_G
Cuts at: 107 146 287 107
Size: 39 141 3860
Fragments arranged by size:
3860 141 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 221 461 1283 221
Size: 240 822 2978
Fragments arranged by size:
2978 822 240
ClaI AT'CG_AT
Cuts at: 223 223
Size: 4040
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 252 1625 1799 2190 2217 3562 252
Size: 1373 174 391 27 1345 730
Fragments arranged by size:
1373 1345 730 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 986 1865 2297 3044 3312 986
Size: 879 432 747 268 1714
Fragments arranged by size:
1714 879 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1179 1179
Size: 4040
EcoRI G'AATT_C
Cuts at: 535 1044 535
Size: 509 3531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 669 2059 669
Size: 1390 2650
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2744 3723 1474
Size: 248 1022 979 1791
Fragments arranged by size:
1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 862 894 925 957 1882 1912 1914 1944 862
Size: 32 31 32 925 30 2 30 2958
Fragments arranged by size:
2958 925 32 32 31 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
HindIII A'AGCT_T
Cuts at: 591 651 591
Size: 60 3980
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 465 654 2711 2895 465
Size: 189 2057 184 1610
Fragments arranged by size:
2057 1610 189 184
MscI TGG'CCA
Cuts at: 254 1801 254
Size: 1547 2493
MslI CAynn'nnrTG
Cuts at: 869 2029 2156 869
Size: 1160 127 2753
Fragments arranged by size:
2753 1160 127
MspA1I CmG'CkG
Cuts at: 615 696 1151 1465 1825 2838 3083 615
Size: 81 455 314 360 1013 245 1572
Fragments arranged by size:
1572 1013 455 360 314 245 81
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NcoI C'CATG_G
Cuts at: 107 146 287 107
Size: 39 141 3860
Fragments arranged by size:
3860 141 39
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 1093 1593 2500 1093
Size: 500 907 2633
Fragments arranged by size:
2633 907 500
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 520 520
Size: 4040
PfoI T'CCnGG_A
Cuts at: 764 764
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PsiI TTA'TAA
Cuts at: 746 746
Size: 4040
PstI C_TGCA'G
Cuts at: 1087 1772 1087
Size: 685 3355
PvuI CG_AT'CG
Cuts at: 104 223 104
Size: 119 3921
PvuII CAG'CTG
Cuts at: 615 1465 1825 615
Size: 850 360 2830
Fragments arranged by size:
2830 850 360
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 1083 1768 2761 2952 33
Size: 1050 685 993 191 1121
Fragments arranged by size:
1121 1050 993 685 191
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 271 1062 271
Size: 791 3249
SmlI C'TyrA_G
Cuts at: 643 1195 2110 2602 2864 643
Size: 552 915 492 262 1819
Fragments arranged by size:
1819 915 552 492 262
SpeI A'CTAG_T
Cuts at: 67 505 67
Size: 438 3602
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StyI C'CwwG_G
Cuts at: 107 146 287 340 1174 1528 107
Size: 39 141 53 834 354 2619
Fragments arranged by size:
2619 834 354 141 53 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 746 778 3557 3589 746
Size: 32 2779 32 1197
Fragments arranged by size:
2779 1197 32 32
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 859 1071 859
Size: 212 3828
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XmaI C'CCGG_G
Cuts at: 269 1060 269
Size: 791 3249
XmnI GAAnn'nnTTC
Cuts at: 539 1007 539
Size: 468 3572
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI