Restriction map for
pF1KB6422
(Circular) (Six-base) MAPSORT of: pF1KB6422.seq Check: 1389 from: 1 to: 4130
pF1KB6422 4130 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 544 544
Size: 4130
AccI GT'mk_AC
Cuts at: 770 1168 770
Size: 398 3732
Acc65I G'GTAC_C
Cuts at: 598 1146 598
Size: 548 3582
AclI AA'CG_TT
Cuts at: 3970 3970
Size: 4130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 131 1091 1097 1955 2387 3134 3402 131
Size: 960 6 858 432 747 268 859
Fragments arranged by size:
960 859 858 747 432 268 6
AflIII A'CryG_T
Cuts at: 1482 2586 3563 1482
Size: 1104 977 2049
Fragments arranged by size:
2049 1104 977
AgeI A'CCGG_T
Cuts at: 2518 2518
Size: 4130
AlwNI CAG_nnn'CTG
Cuts at: 631 1040 1435 3002 631
Size: 409 395 1567 1759
Fragments arranged by size:
1759 1567 409 395
ApaLI G'TGCA_C
Cuts at: 2900 2900
Size: 4130
ApoI r'AATT_y
Cuts at: 326 379 490 1134 3483 3739 326
Size: 53 111 644 2349 256 717
Fragments arranged by size:
2349 717 644 256 111 53
AseI AT'TA_AT
Cuts at: 20 1094 20
Size: 1074 3056
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
AvaI C'yCGr_G
Cuts at: 683 804 1150 683
Size: 121 346 3663
Fragments arranged by size:
3663 346 121
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 134 167 134
Size: 33 4097
BamHI G'GATC_C
Cuts at: 1155 1155
Size: 4130
BanI G'GyrC_C
Cuts at: 598 1146 1560 1808 1843 598
Size: 548 414 248 35 2885
Fragments arranged by size:
2885 548 414 248 35
BanII G_rGCy'C
Cuts at: 1144 2174 1144
Size: 1030 3100
BbeI G_GCGC'C
Cuts at: 1564 1812 1564
Size: 248 3882
BbsI GAAGACnn'nnnn_
Cuts at: 511 1057 511
Size: 546 3584
BbvCI CC'TCA_GC
Cuts at: 335 335
Size: 4130
BciVI GTATCCnnnnn_n'
Cuts at: 2028 2789 4130 2028
Size: 761 1341 2028
Fragments arranged by size:
2028 1341 761
BclI T'GATC_A
Cuts at: 1650 1650
Size: 4130
BfrBI ATG'CAT
Cuts at: 991 991
Size: 4130
BglI GCCn_nnn'nGGC
Cuts at: 615 615
Size: 4130
BglII A'GATC_T
Cuts at: 512 1645 3682 512
Size: 1133 2037 960
Fragments arranged by size:
2037 1133 960
BlpI GC'TnA_GC
Cuts at: 1242 1242
Size: 4130
Bme1580I G_kGCm'C
Cuts at: 1755 1848 2904 1755
Size: 93 1056 2981
Fragments arranged by size:
2981 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 509 1342 1423 1758 3902 509
Size: 833 81 335 2144 737
Fragments arranged by size:
2144 833 737 335 81
BmtI G_CTAG'C
Cuts at: 529 529
Size: 4130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4130
Bpu10I CC'TnA_GC
Cuts at: 335 335
Size: 4130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 905 1306 2185 2677 2975 905
Size: 401 879 492 298 2060
Fragments arranged by size:
2060 879 492 401 298
BsaI GGTCTCn'nnnn_
Cuts at: 649 3834 649
Size: 3185 945
BsaAI yAC'GTr
Cuts at: 1483 1483
Size: 4130
BsaBI GATnn'nnATC
Cuts at: 6 1644 3681 6
Size: 1638 2037 455
Fragments arranged by size:
2037 1638 455
BsaHI Gr'CG_yC
Cuts at: 1561 1809 2511 4007 1561
Size: 248 702 1496 1684
Fragments arranged by size:
1684 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1319 1840 2518 2792 2939 3350 1319
Size: 521 678 274 147 411 2099
Fragments arranged by size:
2099 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 710 1555 2313 2890 3386 710
Size: 845 758 577 496 1454
Fragments arranged by size:
1454 845 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1672 1718 2926 104
Size: 1568 46 1208 1308
Fragments arranged by size:
1568 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1144 1922 2112 2904 1144
Size: 778 190 792 2370
Fragments arranged by size:
2370 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1144 1755 1848 1922 2112 2174 2904 1144
Size: 611 93 74 190 62 730 2370
Fragments arranged by size:
2370 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3350 3350
Size: 4130
BspHI T'CATG_A
Cuts at: 3306 3306
Size: 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 544 1180 1394 1696 2077 544
Size: 636 214 302 381 2597
Fragments arranged by size:
2597 636 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2422 2476 3960 4125 50
Size: 2372 54 1484 165 55
Fragments arranged by size:
2372 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 119 2042 119
Size: 1923 2207
BsrFI r'CCGG_y
Cuts at: 2128 2309 2518 2128
Size: 181 209 3740
Fragments arranged by size:
3740 209 181
BsrGI T'GTAC_A
Cuts at: 581 581
Size: 4130
BssSI C'ACGA_G
Cuts at: 2401 2759 2401
Size: 358 3772
BstAPI GCAn_nnn'nTGC
Cuts at: 1507 1507
Size: 4130
BstBI TT'CG_AA
Cuts at: 2491 2491
Size: 4130
BstYI r'GATC_y
Cuts at: 1 466 512 1155 1622 1645 1980 2226 3238
Size: 465 46 643 467 23 335 246 1012
Cuts at: 3238 3324 3336 3682 1
Size: 86 12 346 449
Fragments arranged by size:
1012 643 467 465 449 346 335 246
86 46 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 952 966 2080 2261 2424 952
Size: 14 1114 181 163 2658
Fragments arranged by size:
2658 1114 181 163 14
BtsI GCAGTG_nn'
Cuts at: 202 368 632 660 757 899 1373 202
Size: 166 264 28 97 142 474 2959
Fragments arranged by size:
2959 474 264 166 142 97 28
DraI TTT'AAA
Cuts at: 1129 1129
Size: 4130
DrdI GACnn_nn'nnGTC
Cuts at: 1836 2694 1836
Size: 858 3272
EaeI y'GGCC_r
Cuts at: 842 1715 1889 2280 2307 3652 842
Size: 873 174 391 27 1345 1320
Fragments arranged by size:
1345 1320 873 391 174 27
EagI C'GGCC_G
Cuts at: 1715 1715
Size: 4130
EarI CTCTTCn'nnn_
Cuts at: 1017 2153 2363 1017
Size: 1136 210 2784
Fragments arranged by size:
2784 1136 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2338 2648 2794 3940 2338
Size: 310 146 1146 2528
Fragments arranged by size:
2528 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1142 1142
Size: 4130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 131 1091 1097 1955 2387 3134 3402 131
Size: 960 6 858 432 747 268 859
Fragments arranged by size:
960 859 858 747 432 268 6
EcoNI CCTnn'n_nnAGG
Cuts at: 968 968
Size: 4130
EcoO109I rG'GnC_Cy
Cuts at: 1269 1269
Size: 4130
EcoRI G'AATT_C
Cuts at: 379 490 1134 379
Size: 111 644 3375
Fragments arranged by size:
3375 644 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 4130
FspI TGC'GCA
Cuts at: 1911 1911
Size: 4130
HaeII r_GCGC'y
Cuts at: 1564 1812 2834 3813 1564
Size: 248 1022 979 1881
Fragments arranged by size:
1881 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 627 659 962 994 1972 2002 2004
Size: 32 144 32 303 32 978 30 2
Cuts at: 2004 2034 451
Size: 30 2547
Fragments arranged by size:
2547 978 303 144 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1169 1169
Size: 4130
HindIII A'AGCT_T
Cuts at: 162 162
Size: 4130
KasI G'GCGC_C
Cuts at: 1560 1808 1560
Size: 248 3882
KpnI G_GTAC'C
Cuts at: 602 1150 602
Size: 548 3582
MluI A'CGCG_T
Cuts at: 3563 3563
Size: 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2801 2985 2801
Size: 184 3946
MscI TGG'CCA
Cuts at: 844 1891 844
Size: 1047 3083
MslI CAynn'nnrTG
Cuts at: 112 478 2119 2246 112
Size: 366 1641 127 1996
Fragments arranged by size:
1996 1641 366 127
MspA1I CmG'CkG
Cuts at: 1241 1555 1915 2928 3173 1241
Size: 314 360 1013 245 2198
Fragments arranged by size:
2198 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2311 2311
Size: 4130
NarI GG'CG_CC
Cuts at: 1561 1809 1561
Size: 248 3882
NcoI C'CATG_G
Cuts at: 107 107
Size: 4130
NdeI CA'TA_TG
Cuts at: 678 678
Size: 4130
NgoMIV G'CCGG_C
Cuts at: 2309 2309
Size: 4130
NheI G'CTAG_C
Cuts at: 525 525
Size: 4130
NsiI A_TGCA'T
Cuts at: 993 993
Size: 4130
NspI r_CATG'y
Cuts at: 1183 1683 2590 1183
Size: 500 907 2723
Fragments arranged by size:
2723 907 500
PciI A'CATG_T
Cuts at: 2586 2586
Size: 4130
PflMI CCAn_nnn'nTGG
Cuts at: 252 252
Size: 4130
PfoI T'CCnGG_A
Cuts at: 662 662
Size: 4130
PmeI GTTT'AAAC
Cuts at: 1129 1129
Size: 4130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 4098
PspXI vC'TCGA_Gb
Cuts at: 683 683
Size: 4130
PstI C_TGCA'G
Cuts at: 1177 1862 1177
Size: 685 3445
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4130
PvuII CAG'CTG
Cuts at: 1555 1915 1555
Size: 360 3770
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 4130
SacI G_AGCT'C
Cuts at: 1144 1144
Size: 4130
SalI G'TCGA_C
Cuts at: 1167 1167
Size: 4130
SapI GCTCTTCn'nnn_
Cuts at: 2153 2363 2153
Size: 210 3920
SbfI CC_TGCA'GG
Cuts at: 1177 1177
Size: 4130
ScaI AGT'ACT
Cuts at: 3647 3647
Size: 4130
SfcI C'TryA_G
Cuts at: 33 1173 1858 2851 3042 33
Size: 1140 685 993 191 1121
Fragments arranged by size:
1140 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1562 1810 1562
Size: 248 3882
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
SmaI CCC'GGG
Cuts at: 1152 1152
Size: 4130
SmlI C'TyrA_G
Cuts at: 683 920 1285 2200 2692 2954 683
Size: 237 365 915 492 262 1859
Fragments arranged by size:
1859 915 492 365 262 237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4130
SphI G_CATG'C
Cuts at: 1183 1683 1183
Size: 500 3630
SspI AAT'ATT
Cuts at: 294 294
Size: 4130
StyI C'CwwG_G
Cuts at: 107 1264 1618 107
Size: 1157 354 2619
Fragments arranged by size:
2619 1157 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1713 1846 2305 2514 1713
Size: 133 459 209 3329
Fragments arranged by size:
3329 459 209 133
TatI w'GTAC_w
Cuts at: 581 3645 581
Size: 3064 1066
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4098
Tth111I GACn'n_nGTC
Cuts at: 1927 1927
Size: 4130
XbaI T'CTAG_A
Cuts at: 1161 1161
Size: 4130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1625 1625
Size: 4130
XhoI C'TCGA_G
Cuts at: 683 683
Size: 4130
XmaI C'CCGG_G
Cuts at: 1150 1150
Size: 4130
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmeI PpiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BcgI BmgBI BpmI BsaXI BseRI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NotI NruI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XmnI ZraI