Restriction map for
pF1KB6423
(Circular) (Six-base) MAPSORT of: pF1KB6423.seq Check: 1962 from: 1 to: 4040
pF1KB6423 4040 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 760 907 1056 760
Size: 147 149 3744
Fragments arranged by size:
3744 149 147
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 878 1865 2297 3044 3312 326
Size: 552 987 432 747 268 1054
Fragments arranged by size:
1054 987 747 552 432 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 317 498 317
Size: 181 3859
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 375 935 1044 3393 3649 375
Size: 560 109 2349 256 766
Fragments arranged by size:
2349 766 560 256 109
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 760 907 1056 1470 1718 1753 760
Size: 147 149 414 248 35 3047
Fragments arranged by size:
3047 414 248 149 147 35
BanII G_rGCy'C
Cuts at: 428 540 603 1054 2084 428
Size: 112 63 451 1030 2384
Fragments arranged by size:
2384 1030 451 112 63
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbsI GAAGACnn'nnnn_
Cuts at: 447 447
Size: 4040
BciVI GTATCCnnnnn_n'
Cuts at: 422 1938 2699 4040 422
Size: 1516 761 1341 422
Fragments arranged by size:
1516 1341 761 422
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BfrBI ATG'CAT
Cuts at: 294 294
Size: 4040
BglI GCCn_nnn'nGGC
Cuts at: 154 1012 1030 154
Size: 858 18 3164
Fragments arranged by size:
3164 858 18
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 873 1152 873
Size: 279 3761
Bme1580I G_kGCm'C
Cuts at: 1665 1758 2814 1665
Size: 93 1056 2891
Fragments arranged by size:
2891 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BmtI G_CTAG'C
Cuts at: 652 955 652
Size: 303 3737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 521 866 334
Size: 187 345 3508
Fragments arranged by size:
3508 345 187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1465 2223 2800 3296 1465
Size: 758 577 496 2209
Fragments arranged by size:
2209 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 535 706 370
Size: 165 171 3704
Fragments arranged by size:
3704 171 165
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 428 1054 1832 2022 2814 428
Size: 626 778 190 792 1654
Fragments arranged by size:
1654 792 778 626 190
BsmI GAATG_Cn'
Cuts at: 746 746
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 428 540 603 1054 1665 1758 1832 2022 2084
Size: 112 63 451 611 93 74 190 62
Cuts at: 2084 2814 428
Size: 730 1654
Fragments arranged by size:
1654 730 611 451 190 112 93 74
63 62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 3216 3216
Size: 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1090 1304 1606 1987 1090
Size: 214 302 381 3143
Fragments arranged by size:
3143 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 475 1952 475
Size: 1477 2563
BsrFI r'CCGG_y
Cuts at: 230 2038 2219 2428 230
Size: 1808 181 209 1842
Fragments arranged by size:
1842 1808 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 548 2401 548
Size: 1853 2187
BstXI CCAn_nnnn'nTGG
Cuts at: 562 562
Size: 4040
BstYI r'GATC_y
Cuts at: 1 1065 1532 1555 1890 2136 3148 3234 3246
Size: 1064 467 23 335 246 1012 86 12
Cuts at: 3246 3592 1
Size: 346 449
Fragments arranged by size:
1064 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 962 962
Size: 4040
BtgI C'CryG_G
Cuts at: 107 561 943 107
Size: 454 382 3204
Fragments arranged by size:
3204 454 382
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 487 785 1283 487
Size: 298 498 3244
Fragments arranged by size:
3244 498 298
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DraIII CAC_nnn'GTG
Cuts at: 636 636
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 1625 1799 2190 2217 3562 1625
Size: 174 391 27 1345 2103
Fragments arranged by size:
2103 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 426 1052 426
Size: 626 3414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 334 521 866 878 1865 2297 3044 3312
Size: 8 187 345 12 987 432 747 268
Cuts at: 3312 326
Size: 1054
Fragments arranged by size:
1054 987 747 432 345 268 187 12
8
EcoO109I rG'GnC_Cy
Cuts at: 589 966 1179 589
Size: 377 213 3450
Fragments arranged by size:
3450 377 213
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2744 3723 1474
Size: 248 1022 979 1791
Fragments arranged by size:
1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 413 445 590 622 1882 1912 1914 1944 413
Size: 32 145 32 1260 30 2 30 2509
Fragments arranged by size:
2509 1260 145 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 764 911 1060 764
Size: 147 149 3744
Fragments arranged by size:
3744 149 147
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 295 480 560 2029 2156 295
Size: 185 80 1469 127 2179
Fragments arranged by size:
2179 1469 185 127 80
MspA1I CmG'CkG
Cuts at: 904 997 1151 1465 1825 2838 3083 904
Size: 93 154 314 360 1013 245 1861
Fragments arranged by size:
1861 1013 360 314 245 154 93
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NcoI C'CATG_G
Cuts at: 107 561 107
Size: 454 3586
NdeI CA'TA_TG
Cuts at: 296 404 296
Size: 108 3932
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NheI G'CTAG_C
Cuts at: 648 951 648
Size: 303 3737
NsiI A_TGCA'T
Cuts at: 296 296
Size: 4040
NspI r_CATG'y
Cuts at: 1093 1593 2500 1093
Size: 500 907 2633
Fragments arranged by size:
2633 907 500
PasI CC'CwG_GG
Cuts at: 438 816 438
Size: 378 3662
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PpuMI rG'GwC_Cy
Cuts at: 589 589
Size: 4040
PstI C_TGCA'G
Cuts at: 665 1087 1772 665
Size: 422 685 2933
Fragments arranged by size:
2933 685 422
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 904 1465 1825 904
Size: 561 360 3119
Fragments arranged by size:
3119 561 360
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 428 1054 428
Size: 626 3414
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SanDI GG'GwC_CC
Cuts at: 589 589
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 661 1083 1768 2761 2952 33
Size: 628 422 685 993 191 1121
Fragments arranged by size:
1121 993 685 628 422 191
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 1195 2110 2602 2864 1195
Size: 915 492 262 2371
Fragments arranged by size:
2371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StyI C'CwwG_G
Cuts at: 107 561 1174 1528 107
Size: 454 613 354 2619
Fragments arranged by size:
2619 613 454 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 497 529 3557 3589 497
Size: 32 3028 32 948
Fragments arranged by size:
3028 948 32 32
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
XmnI GAAnn'nnTTC
Cuts at: 378 938 378
Size: 560 3480
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI Bpu10I BsaXI
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NotI NruI PacI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI