Restriction map for pF1KB6424
 (Circular) (Six-base) MAPSORT of: pF1KB6424.seq  Check: 4316  from: 1  to: 4133



pF1KB6424  4133 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1012    1012 
   Size:       4133
 
AccI GT'mk_AC

Cuts at:    762    1171     762 
   Size:        409    3724
 
Acc65I G'GTAC_C

Cuts at:   1149    1149 
   Size:       4133
 
AclI AA'CG_TT

Cuts at:   3973    3973 
   Size:       4133
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1958    2390    3137    3405    1958 
   Size:        432     747     268    2686

  Fragments arranged by size: 

                2686     747     432     268
 
AflIII A'CryG_T

Cuts at:    780    1485    2589    3566     780 
   Size:        705    1104     977    1347

  Fragments arranged by size: 

                1347    1104     977     705
 
AgeI A'CCGG_T

Cuts at:   2521    2521 
   Size:       4133
 
AlwNI CAG_nnn'CTG

Cuts at:   1438    3005    1438 
   Size:       1567    2566
 
ApaLI G'TGCA_C

Cuts at:   2903    2903 
   Size:       4133
 
ApoI r'AATT_y

Cuts at:   1137    3486    3742    1137 
   Size:       2349     256    1528

  Fragments arranged by size: 

                2349    1528     256
 
AseI AT'TA_AT

Cuts at:     20     343      20 
   Size:        323    3810
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4133
 
AvaI C'yCGr_G

Cuts at:   1153    1153 
   Size:       4133
 
BamHI G'GATC_C

Cuts at:   1158    1158 
   Size:       4133
 
BanI G'GyrC_C

Cuts at:   1149    1563    1811    1846    1149 
   Size:        414     248      35    3436

  Fragments arranged by size: 

                3436     414     248      35
 
BanII G_rGCy'C

Cuts at:   1147    2177    1147 
   Size:       1030    3103
 
BbeI G_GCGC'C

Cuts at:   1567    1815    1567 
   Size:        248    3885
 
BciVI GTATCCnnnnn_n'

Cuts at:    292    2031    2792    4133     292 
   Size:       1739     761    1341     292

  Fragments arranged by size: 

                1739    1341     761     292
 
BclI T'GATC_A

Cuts at:   1653    1653 
   Size:       4133
 
BfrBI ATG'CAT

Cuts at:    291     378     291 
   Size:         87    4046
 
BglII A'GATC_T

Cuts at:   1648    3685    1648 
   Size:       2037    2096
 
BlpI GC'TnA_GC

Cuts at:   1245    1245 
   Size:       4133
 
Bme1580I G_kGCm'C

Cuts at:   1758    1851    2907    1758 
   Size:         93    1056    2984

  Fragments arranged by size: 

                2984    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    659    1345    1426    1761    3905     659 
   Size:        686      81     335    2144     887

  Fragments arranged by size: 

                2144     887     686     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2500    2500 
   Size:       4133
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    680     680 
   Size:       4133
 
Bpu10I CC'TnA_GC

Cuts at:   1059    1059 
   Size:       4133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    314    1309    2188    2680    2978     314 
   Size:        995     879     492     298    1469

  Fragments arranged by size: 

                1469     995     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3837    3837 
   Size:       4133
 
BsaAI yAC'GTr

Cuts at:   1486    1486 
   Size:       4133
 
BsaBI GATnn'nnATC

Cuts at:      6    1647    3684       6 
   Size:       1641    2037     455

  Fragments arranged by size: 

                2037    1641     455
 
BsaHI Gr'CG_yC

Cuts at:    954    1564    1812    2514    4010     954 
   Size:        610     248     702    1496    1077

  Fragments arranged by size: 

                1496    1077     702     610     248
 
BsaWI w'CCGG_w

Cuts at:   1322    1843    2521    2795    2942    3353    1322 
   Size:        521     678     274     147     411    2102

  Fragments arranged by size: 

                2102     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    629    1558    2316    2893    3389     629 
   Size:        929     758     577     496    1373

  Fragments arranged by size: 

                1373     929     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    896    1040     896 
   Size:        144    3989
 
BsiEI CG_ry'CG

Cuts at:    104    1675    1721    2929     104 
   Size:       1571      46    1208    1308

  Fragments arranged by size: 

                1571    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1147    1925    2115    2907    1147 
   Size:        778     190     792    2373

  Fragments arranged by size: 

                2373     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    654     654 
   Size:       4133
 
BsmBI CGTCTCn'nnnn_

Cuts at:    961     961 
   Size:       4133
 
Bsp1286I G_dGCh'C

Cuts at:   1147    1758    1851    1925    2115    2177    2907    1147 
   Size:        611      93      74     190      62     730    2373

  Fragments arranged by size: 

                2373     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3353    3353 
   Size:       4133
 
BspHI T'CATG_A

Cuts at:    903    1038    3309     903 
   Size:        135    2271    1727

  Fragments arranged by size: 

                2271    1727     135
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1012    1183    1397    1699    2080    1012 
   Size:        171     214     302     381    3065

  Fragments arranged by size: 

                3065     381     302     214     171
 
BsrBI CCG'CTC

Cuts at:     50    2425    2479    3963    4128      50 
   Size:       2375      54    1484     165      55

  Fragments arranged by size: 

                2375    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2045    2045 
   Size:       4133
 
BsrFI r'CCGG_y

Cuts at:    857    2131    2312    2521     857 
   Size:       1274     181     209    2469

  Fragments arranged by size: 

                2469    1274     209     181
 
BssSI C'ACGA_G

Cuts at:   2404    2762    2404 
   Size:        358    3775
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1510    1510 
   Size:       4133
 
BstBI TT'CG_AA

Cuts at:   2494    2494 
   Size:       4133
 
BstYI r'GATC_y

Cuts at:      1    1158    1625    1648    1983    2229    3241    3327    3339 
   Size:       1157     467      23     335     246    1012      86      12

Cuts at:   3339    3685       1 
   Size:        346     449

  Fragments arranged by size: 

                1157    1012     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2083    2264    2427    2083 
   Size:        181     163    3789

  Fragments arranged by size: 

                3789     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1376    1376 
   Size:       4133
 
DraI TTT'AAA

Cuts at:   1132    1132 
   Size:       4133
 
DrdI GACnn_nn'nnGTC

Cuts at:   1839    2697    1839 
   Size:        858    3275
 
EaeI y'GGCC_r

Cuts at:   1718    1892    2283    2310    3655    1718 
   Size:        174     391      27    1345    2196

  Fragments arranged by size: 

                2196    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1718    1718 
   Size:       4133
 
EarI CTCTTCn'nnn_

Cuts at:    732    2156    2366     732 
   Size:       1424     210    2499

  Fragments arranged by size: 

                2499    1424     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2341    2651    2797    3943    2341 
   Size:        310     146    1146    2531

  Fragments arranged by size: 

                2531    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1145    1145 
   Size:       4133
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    680    1958    2390    3137    3405     680 
   Size:       1278     432     747     268    1408

  Fragments arranged by size: 

                1408    1278     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    395    1272     395 
   Size:        877    3256
 
EcoRI G'AATT_C

Cuts at:   1137    1137 
   Size:       4133
 
EcoRV GAT'ATC

Cuts at:    792     792 
   Size:       4133
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2152    2152 
   Size:       4133
 
FspI TGC'GCA

Cuts at:   1914    1914 
   Size:       4133
 
HaeII r_GCGC'y

Cuts at:   1567    1815    2837    3816    1567 
   Size:        248    1022     979    1884

  Fragments arranged by size: 

                1884    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1975    2005    2007    2037    1975 
   Size:         30       2      30    4071

  Fragments arranged by size: 

                4071      30      30       2
 
HincII GTy'rAC

Cuts at:   1172    1172 
   Size:       4133
 
KasI G'GCGC_C

Cuts at:   1563    1811    1563 
   Size:        248    3885
 
KpnI G_GTAC'C

Cuts at:   1153    1153 
   Size:       4133
 
MluI A'CGCG_T

Cuts at:   3566    3566 
   Size:       4133
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2804    2988    2804 
   Size:        184    3949
 
MscI TGG'CCA

Cuts at:   1894    1894 
   Size:       4133
 
MslI CAynn'nnrTG

Cuts at:   1084    2122    2249    1084 
   Size:       1038     127    2968

  Fragments arranged by size: 

                2968    1038     127
 
MspA1I CmG'CkG

Cuts at:   1244    1558    1918    2931    3176    1244 
   Size:        314     360    1013     245    2201

  Fragments arranged by size: 

                2201    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:    859    2314     859 
   Size:       1455    2678
 
NarI GG'CG_CC

Cuts at:   1564    1812    1564 
   Size:        248    3885
 
NgoMIV G'CCGG_C

Cuts at:    857    2312     857 
   Size:       1455    2678
 
NsiI A_TGCA'T

Cuts at:    293     380     293 
   Size:         87    4046
 
NspI r_CATG'y

Cuts at:    182     382     784    1186    1686    2593     182 
   Size:        200     402     402     500     907    1722

  Fragments arranged by size: 

                1722     907     500     402     402     200
 
PciI A'CATG_T

Cuts at:    780    2589     780 
   Size:       1809    2324
 
PflMI CCAn_nnn'nTGG

Cuts at:    737     737 
   Size:       4133
 
PmeI GTTT'AAAC

Cuts at:   1132    1132 
   Size:       4133
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3272    3304    3272 
   Size:         32    4101
 
PpuMI rG'GwC_Cy

Cuts at:    395     395 
   Size:       4133
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    237     269     237 
   Size:         32    4101
 
PstI C_TGCA'G

Cuts at:   1180    1865    1180 
   Size:        685    3448
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4133
 
PvuII CAG'CTG

Cuts at:   1558    1918    1558 
   Size:        360    3773
 
RsrII CG'GwC_CG

Cuts at:   2328    2328 
   Size:       4133
 
SacI G_AGCT'C

Cuts at:   1147    1147 
   Size:       4133
 
SalI G'TCGA_C

Cuts at:   1170    1170 
   Size:       4133
 
SapI GCTCTTCn'nnn_

Cuts at:   2156    2366    2156 
   Size:        210    3923
 
SbfI CC_TGCA'GG

Cuts at:   1180    1180 
   Size:       4133
 
ScaI AGT'ACT

Cuts at:   3650    3650 
   Size:       4133
 
SfcI C'TryA_G

Cuts at:     33     932    1176    1861    2854    3045      33 
   Size:        899     244     685     993     191    1121

  Fragments arranged by size: 

                1121     993     899     685     244     191
 
SfoI GGC'GCC

Cuts at:   1565    1813    1565 
   Size:        248    3885
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4133
 
SmaI CCC'GGG

Cuts at:   1155    1155 
   Size:       4133
 
SmlI C'TyrA_G

Cuts at:    329    1288    2203    2695    2957     329 
   Size:        959     915     492     262    1505

  Fragments arranged by size: 

                1505     959     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4133
 
SphI G_CATG'C

Cuts at:    382    1186    1686     382 
   Size:        804     500    2829

  Fragments arranged by size: 

                2829     804     500
 
StyI C'CwwG_G

Cuts at:   1267    1621    1267 
   Size:        354    3779
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    393    1716    1849    2308    2517     393 
   Size:       1323     133     459     209    2009

  Fragments arranged by size: 

                2009    1323     459     209     133
 
TatI w'GTAC_w

Cuts at:   3648    3648 
   Size:       4133
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3650    3682    3650 
   Size:         32    4101
 
Tth111I GACn'n_nGTC

Cuts at:   1930    1930 
   Size:       4133
 
XbaI T'CTAG_A

Cuts at:   1164    1164 
   Size:       4133
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114    1628     114 
   Size:       1514    2619
 
XmaI C'CCGG_G

Cuts at:   1153    1153 
   Size:       4133
 
XmnI GAAnn'nnTTC

Cuts at:    927     927 
   Size:       4133
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI     NspI
     PciI    PflMI     PmeI     PpiI    PpuMI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI     BglI    BmgBI
     BmtI    BsaXI    BseRI    BsiWI    BsrGI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII    EcoNI     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     PacI     PasI     PfoI     PmlI    PshAI     PsiI   PspOMI
    PspXI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     ZraI
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