(Circular) (Six-base) MAPSORT of: pF1KB6424.seq Check: 4316 from: 1 to: 4133 pF1KB6424 4133 bp With 182 enzymes: SgfI * March 2, 2010 14:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1012 1012 Size: 4133 AccI GT'mk_AC Cuts at: 762 1171 762 Size: 409 3724 Acc65I G'GTAC_C Cuts at: 1149 1149 Size: 4133 AclI AA'CG_TT Cuts at: 3973 3973 Size: 4133 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1958 2390 3137 3405 1958 Size: 432 747 268 2686 Fragments arranged by size: 2686 747 432 268 AflIII A'CryG_T Cuts at: 780 1485 2589 3566 780 Size: 705 1104 977 1347 Fragments arranged by size: 1347 1104 977 705 AgeI A'CCGG_T Cuts at: 2521 2521 Size: 4133 AlwNI CAG_nnn'CTG Cuts at: 1438 3005 1438 Size: 1567 2566 ApaLI G'TGCA_C Cuts at: 2903 2903 Size: 4133 ApoI r'AATT_y Cuts at: 1137 3486 3742 1137 Size: 2349 256 1528 Fragments arranged by size: 2349 1528 256 AseI AT'TA_AT Cuts at: 20 343 20 Size: 323 3810 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4133 AvaI C'yCGr_G Cuts at: 1153 1153 Size: 4133 BamHI G'GATC_C Cuts at: 1158 1158 Size: 4133 BanI G'GyrC_C Cuts at: 1149 1563 1811 1846 1149 Size: 414 248 35 3436 Fragments arranged by size: 3436 414 248 35 BanII G_rGCy'C Cuts at: 1147 2177 1147 Size: 1030 3103 BbeI G_GCGC'C Cuts at: 1567 1815 1567 Size: 248 3885 BciVI GTATCCnnnnn_n' Cuts at: 292 2031 2792 4133 292 Size: 1739 761 1341 292 Fragments arranged by size: 1739 1341 761 292 BclI T'GATC_A Cuts at: 1653 1653 Size: 4133 BfrBI ATG'CAT Cuts at: 291 378 291 Size: 87 4046 BglII A'GATC_T Cuts at: 1648 3685 1648 Size: 2037 2096 BlpI GC'TnA_GC Cuts at: 1245 1245 Size: 4133 Bme1580I G_kGCm'C Cuts at: 1758 1851 2907 1758 Size: 93 1056 2984 Fragments arranged by size: 2984 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 659 1345 1426 1761 3905 659 Size: 686 81 335 2144 887 Fragments arranged by size: 2144 887 686 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2500 2500 Size: 4133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 680 680 Size: 4133 Bpu10I CC'TnA_GC Cuts at: 1059 1059 Size: 4133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 314 1309 2188 2680 2978 314 Size: 995 879 492 298 1469 Fragments arranged by size: 1469 995 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3837 3837 Size: 4133 BsaAI yAC'GTr Cuts at: 1486 1486 Size: 4133 BsaBI GATnn'nnATC Cuts at: 6 1647 3684 6 Size: 1641 2037 455 Fragments arranged by size: 2037 1641 455 BsaHI Gr'CG_yC Cuts at: 954 1564 1812 2514 4010 954 Size: 610 248 702 1496 1077 Fragments arranged by size: 1496 1077 702 610 248 BsaWI w'CCGG_w Cuts at: 1322 1843 2521 2795 2942 3353 1322 Size: 521 678 274 147 411 2102 Fragments arranged by size: 2102 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 629 1558 2316 2893 3389 629 Size: 929 758 577 496 1373 Fragments arranged by size: 1373 929 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 896 1040 896 Size: 144 3989 BsiEI CG_ry'CG Cuts at: 104 1675 1721 2929 104 Size: 1571 46 1208 1308 Fragments arranged by size: 1571 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1147 1925 2115 2907 1147 Size: 778 190 792 2373 Fragments arranged by size: 2373 792 778 190 BsmI GAATG_Cn' Cuts at: 654 654 Size: 4133 BsmBI CGTCTCn'nnnn_ Cuts at: 961 961 Size: 4133 Bsp1286I G_dGCh'C Cuts at: 1147 1758 1851 1925 2115 2177 2907 1147 Size: 611 93 74 190 62 730 2373 Fragments arranged by size: 2373 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3353 3353 Size: 4133 BspHI T'CATG_A Cuts at: 903 1038 3309 903 Size: 135 2271 1727 Fragments arranged by size: 2271 1727 135 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1012 1183 1397 1699 2080 1012 Size: 171 214 302 381 3065 Fragments arranged by size: 3065 381 302 214 171 BsrBI CCG'CTC Cuts at: 50 2425 2479 3963 4128 50 Size: 2375 54 1484 165 55 Fragments arranged by size: 2375 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2045 2045 Size: 4133 BsrFI r'CCGG_y Cuts at: 857 2131 2312 2521 857 Size: 1274 181 209 2469 Fragments arranged by size: 2469 1274 209 181 BssSI C'ACGA_G Cuts at: 2404 2762 2404 Size: 358 3775 BstAPI GCAn_nnn'nTGC Cuts at: 1510 1510 Size: 4133 BstBI TT'CG_AA Cuts at: 2494 2494 Size: 4133 BstYI r'GATC_y Cuts at: 1 1158 1625 1648 1983 2229 3241 3327 3339 Size: 1157 467 23 335 246 1012 86 12 Cuts at: 3339 3685 1 Size: 346 449 Fragments arranged by size: 1157 1012 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2083 2264 2427 2083 Size: 181 163 3789 Fragments arranged by size: 3789 181 163 BtsI GCAGTG_nn' Cuts at: 1376 1376 Size: 4133 DraI TTT'AAA Cuts at: 1132 1132 Size: 4133 DrdI GACnn_nn'nnGTC Cuts at: 1839 2697 1839 Size: 858 3275 EaeI y'GGCC_r Cuts at: 1718 1892 2283 2310 3655 1718 Size: 174 391 27 1345 2196 Fragments arranged by size: 2196 1345 391 174 27 EagI C'GGCC_G Cuts at: 1718 1718 Size: 4133 EarI CTCTTCn'nnn_ Cuts at: 732 2156 2366 732 Size: 1424 210 2499 Fragments arranged by size: 2499 1424 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2341 2651 2797 3943 2341 Size: 310 146 1146 2531 Fragments arranged by size: 2531 1146 310 146 EcoICRI GAG'CTC Cuts at: 1145 1145 Size: 4133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 680 1958 2390 3137 3405 680 Size: 1278 432 747 268 1408 Fragments arranged by size: 1408 1278 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 395 1272 395 Size: 877 3256 EcoRI G'AATT_C Cuts at: 1137 1137 Size: 4133 EcoRV GAT'ATC Cuts at: 792 792 Size: 4133 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2152 2152 Size: 4133 FspI TGC'GCA Cuts at: 1914 1914 Size: 4133 HaeII r_GCGC'y Cuts at: 1567 1815 2837 3816 1567 Size: 248 1022 979 1884 Fragments arranged by size: 1884 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1975 2005 2007 2037 1975 Size: 30 2 30 4071 Fragments arranged by size: 4071 30 30 2 HincII GTy'rAC Cuts at: 1172 1172 Size: 4133 KasI G'GCGC_C Cuts at: 1563 1811 1563 Size: 248 3885 KpnI G_GTAC'C Cuts at: 1153 1153 Size: 4133 MluI A'CGCG_T Cuts at: 3566 3566 Size: 4133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2804 2988 2804 Size: 184 3949 MscI TGG'CCA Cuts at: 1894 1894 Size: 4133 MslI CAynn'nnrTG Cuts at: 1084 2122 2249 1084 Size: 1038 127 2968 Fragments arranged by size: 2968 1038 127 MspA1I CmG'CkG Cuts at: 1244 1558 1918 2931 3176 1244 Size: 314 360 1013 245 2201 Fragments arranged by size: 2201 1013 360 314 245 NaeI GCC'GGC Cuts at: 859 2314 859 Size: 1455 2678 NarI GG'CG_CC Cuts at: 1564 1812 1564 Size: 248 3885 NgoMIV G'CCGG_C Cuts at: 857 2312 857 Size: 1455 2678 NsiI A_TGCA'T Cuts at: 293 380 293 Size: 87 4046 NspI r_CATG'y Cuts at: 182 382 784 1186 1686 2593 182 Size: 200 402 402 500 907 1722 Fragments arranged by size: 1722 907 500 402 402 200 PciI A'CATG_T Cuts at: 780 2589 780 Size: 1809 2324 PflMI CCAn_nnn'nTGG Cuts at: 737 737 Size: 4133 PmeI GTTT'AAAC Cuts at: 1132 1132 Size: 4133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3272 3304 3272 Size: 32 4101 PpuMI rG'GwC_Cy Cuts at: 395 395 Size: 4133 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 237 269 237 Size: 32 4101 PstI C_TGCA'G Cuts at: 1180 1865 1180 Size: 685 3448 PvuI CG_AT'CG Cuts at: 104 104 Size: 4133 PvuII CAG'CTG Cuts at: 1558 1918 1558 Size: 360 3773 RsrII CG'GwC_CG Cuts at: 2328 2328 Size: 4133 SacI G_AGCT'C Cuts at: 1147 1147 Size: 4133 SalI G'TCGA_C Cuts at: 1170 1170 Size: 4133 SapI GCTCTTCn'nnn_ Cuts at: 2156 2366 2156 Size: 210 3923 SbfI CC_TGCA'GG Cuts at: 1180 1180 Size: 4133 ScaI AGT'ACT Cuts at: 3650 3650 Size: 4133 SfcI C'TryA_G Cuts at: 33 932 1176 1861 2854 3045 33 Size: 899 244 685 993 191 1121 Fragments arranged by size: 1121 993 899 685 244 191 SfoI GGC'GCC Cuts at: 1565 1813 1565 Size: 248 3885 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4133 SmaI CCC'GGG Cuts at: 1155 1155 Size: 4133 SmlI C'TyrA_G Cuts at: 329 1288 2203 2695 2957 329 Size: 959 915 492 262 1505 Fragments arranged by size: 1505 959 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4133 SphI G_CATG'C Cuts at: 382 1186 1686 382 Size: 804 500 2829 Fragments arranged by size: 2829 804 500 StyI C'CwwG_G Cuts at: 1267 1621 1267 Size: 354 3779 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 393 1716 1849 2308 2517 393 Size: 1323 133 459 209 2009 Fragments arranged by size: 2009 1323 459 209 133 TatI w'GTAC_w Cuts at: 3648 3648 Size: 4133 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3650 3682 3650 Size: 32 4101 Tth111I GACn'n_nGTC Cuts at: 1930 1930 Size: 4133 XbaI T'CTAG_A Cuts at: 1164 1164 Size: 4133 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 1628 114 Size: 1514 2619 XmaI C'CCGG_G Cuts at: 1153 1153 Size: 4133 XmnI GAAnn'nnTTC Cuts at: 927 927 Size: 4133 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI PflMI PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI BsaXI BseRI BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI