Restriction map for
pF1KB6424
(Circular) (Six-base) MAPSORT of: pF1KB6424.seq Check: 4316 from: 1 to: 4133
pF1KB6424 4133 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1012 1012
Size: 4133
AccI GT'mk_AC
Cuts at: 762 1171 762
Size: 409 3724
Acc65I G'GTAC_C
Cuts at: 1149 1149
Size: 4133
AclI AA'CG_TT
Cuts at: 3973 3973
Size: 4133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1958 2390 3137 3405 1958
Size: 432 747 268 2686
Fragments arranged by size:
2686 747 432 268
AflIII A'CryG_T
Cuts at: 780 1485 2589 3566 780
Size: 705 1104 977 1347
Fragments arranged by size:
1347 1104 977 705
AgeI A'CCGG_T
Cuts at: 2521 2521
Size: 4133
AlwNI CAG_nnn'CTG
Cuts at: 1438 3005 1438
Size: 1567 2566
ApaLI G'TGCA_C
Cuts at: 2903 2903
Size: 4133
ApoI r'AATT_y
Cuts at: 1137 3486 3742 1137
Size: 2349 256 1528
Fragments arranged by size:
2349 1528 256
AseI AT'TA_AT
Cuts at: 20 343 20
Size: 323 3810
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
AvaI C'yCGr_G
Cuts at: 1153 1153
Size: 4133
BamHI G'GATC_C
Cuts at: 1158 1158
Size: 4133
BanI G'GyrC_C
Cuts at: 1149 1563 1811 1846 1149
Size: 414 248 35 3436
Fragments arranged by size:
3436 414 248 35
BanII G_rGCy'C
Cuts at: 1147 2177 1147
Size: 1030 3103
BbeI G_GCGC'C
Cuts at: 1567 1815 1567
Size: 248 3885
BciVI GTATCCnnnnn_n'
Cuts at: 292 2031 2792 4133 292
Size: 1739 761 1341 292
Fragments arranged by size:
1739 1341 761 292
BclI T'GATC_A
Cuts at: 1653 1653
Size: 4133
BfrBI ATG'CAT
Cuts at: 291 378 291
Size: 87 4046
BglII A'GATC_T
Cuts at: 1648 3685 1648
Size: 2037 2096
BlpI GC'TnA_GC
Cuts at: 1245 1245
Size: 4133
Bme1580I G_kGCm'C
Cuts at: 1758 1851 2907 1758
Size: 93 1056 2984
Fragments arranged by size:
2984 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 659 1345 1426 1761 3905 659
Size: 686 81 335 2144 887
Fragments arranged by size:
2144 887 686 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 4133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 680
Size: 4133
Bpu10I CC'TnA_GC
Cuts at: 1059 1059
Size: 4133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 1309 2188 2680 2978 314
Size: 995 879 492 298 1469
Fragments arranged by size:
1469 995 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3837 3837
Size: 4133
BsaAI yAC'GTr
Cuts at: 1486 1486
Size: 4133
BsaBI GATnn'nnATC
Cuts at: 6 1647 3684 6
Size: 1641 2037 455
Fragments arranged by size:
2037 1641 455
BsaHI Gr'CG_yC
Cuts at: 954 1564 1812 2514 4010 954
Size: 610 248 702 1496 1077
Fragments arranged by size:
1496 1077 702 610 248
BsaWI w'CCGG_w
Cuts at: 1322 1843 2521 2795 2942 3353 1322
Size: 521 678 274 147 411 2102
Fragments arranged by size:
2102 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 629 1558 2316 2893 3389 629
Size: 929 758 577 496 1373
Fragments arranged by size:
1373 929 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 896 1040 896
Size: 144 3989
BsiEI CG_ry'CG
Cuts at: 104 1675 1721 2929 104
Size: 1571 46 1208 1308
Fragments arranged by size:
1571 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1147 1925 2115 2907 1147
Size: 778 190 792 2373
Fragments arranged by size:
2373 792 778 190
BsmI GAATG_Cn'
Cuts at: 654 654
Size: 4133
BsmBI CGTCTCn'nnnn_
Cuts at: 961 961
Size: 4133
Bsp1286I G_dGCh'C
Cuts at: 1147 1758 1851 1925 2115 2177 2907 1147
Size: 611 93 74 190 62 730 2373
Fragments arranged by size:
2373 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3353 3353
Size: 4133
BspHI T'CATG_A
Cuts at: 903 1038 3309 903
Size: 135 2271 1727
Fragments arranged by size:
2271 1727 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1012 1183 1397 1699 2080 1012
Size: 171 214 302 381 3065
Fragments arranged by size:
3065 381 302 214 171
BsrBI CCG'CTC
Cuts at: 50 2425 2479 3963 4128 50
Size: 2375 54 1484 165 55
Fragments arranged by size:
2375 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2045 2045
Size: 4133
BsrFI r'CCGG_y
Cuts at: 857 2131 2312 2521 857
Size: 1274 181 209 2469
Fragments arranged by size:
2469 1274 209 181
BssSI C'ACGA_G
Cuts at: 2404 2762 2404
Size: 358 3775
BstAPI GCAn_nnn'nTGC
Cuts at: 1510 1510
Size: 4133
BstBI TT'CG_AA
Cuts at: 2494 2494
Size: 4133
BstYI r'GATC_y
Cuts at: 1 1158 1625 1648 1983 2229 3241 3327 3339
Size: 1157 467 23 335 246 1012 86 12
Cuts at: 3339 3685 1
Size: 346 449
Fragments arranged by size:
1157 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2083 2264 2427 2083
Size: 181 163 3789
Fragments arranged by size:
3789 181 163
BtsI GCAGTG_nn'
Cuts at: 1376 1376
Size: 4133
DraI TTT'AAA
Cuts at: 1132 1132
Size: 4133
DrdI GACnn_nn'nnGTC
Cuts at: 1839 2697 1839
Size: 858 3275
EaeI y'GGCC_r
Cuts at: 1718 1892 2283 2310 3655 1718
Size: 174 391 27 1345 2196
Fragments arranged by size:
2196 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1718 1718
Size: 4133
EarI CTCTTCn'nnn_
Cuts at: 732 2156 2366 732
Size: 1424 210 2499
Fragments arranged by size:
2499 1424 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2341 2651 2797 3943 2341
Size: 310 146 1146 2531
Fragments arranged by size:
2531 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1145 1145
Size: 4133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 1958 2390 3137 3405 680
Size: 1278 432 747 268 1408
Fragments arranged by size:
1408 1278 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 395 1272 395
Size: 877 3256
EcoRI G'AATT_C
Cuts at: 1137 1137
Size: 4133
EcoRV GAT'ATC
Cuts at: 792 792
Size: 4133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 4133
FspI TGC'GCA
Cuts at: 1914 1914
Size: 4133
HaeII r_GCGC'y
Cuts at: 1567 1815 2837 3816 1567
Size: 248 1022 979 1884
Fragments arranged by size:
1884 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1975 2005 2007 2037 1975
Size: 30 2 30 4071
Fragments arranged by size:
4071 30 30 2
HincII GTy'rAC
Cuts at: 1172 1172
Size: 4133
KasI G'GCGC_C
Cuts at: 1563 1811 1563
Size: 248 3885
KpnI G_GTAC'C
Cuts at: 1153 1153
Size: 4133
MluI A'CGCG_T
Cuts at: 3566 3566
Size: 4133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2804 2988 2804
Size: 184 3949
MscI TGG'CCA
Cuts at: 1894 1894
Size: 4133
MslI CAynn'nnrTG
Cuts at: 1084 2122 2249 1084
Size: 1038 127 2968
Fragments arranged by size:
2968 1038 127
MspA1I CmG'CkG
Cuts at: 1244 1558 1918 2931 3176 1244
Size: 314 360 1013 245 2201
Fragments arranged by size:
2201 1013 360 314 245
NaeI GCC'GGC
Cuts at: 859 2314 859
Size: 1455 2678
NarI GG'CG_CC
Cuts at: 1564 1812 1564
Size: 248 3885
NgoMIV G'CCGG_C
Cuts at: 857 2312 857
Size: 1455 2678
NsiI A_TGCA'T
Cuts at: 293 380 293
Size: 87 4046
NspI r_CATG'y
Cuts at: 182 382 784 1186 1686 2593 182
Size: 200 402 402 500 907 1722
Fragments arranged by size:
1722 907 500 402 402 200
PciI A'CATG_T
Cuts at: 780 2589 780
Size: 1809 2324
PflMI CCAn_nnn'nTGG
Cuts at: 737 737
Size: 4133
PmeI GTTT'AAAC
Cuts at: 1132 1132
Size: 4133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 4101
PpuMI rG'GwC_Cy
Cuts at: 395 395
Size: 4133
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 237 269 237
Size: 32 4101
PstI C_TGCA'G
Cuts at: 1180 1865 1180
Size: 685 3448
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4133
PvuII CAG'CTG
Cuts at: 1558 1918 1558
Size: 360 3773
RsrII CG'GwC_CG
Cuts at: 2328 2328
Size: 4133
SacI G_AGCT'C
Cuts at: 1147 1147
Size: 4133
SalI G'TCGA_C
Cuts at: 1170 1170
Size: 4133
SapI GCTCTTCn'nnn_
Cuts at: 2156 2366 2156
Size: 210 3923
SbfI CC_TGCA'GG
Cuts at: 1180 1180
Size: 4133
ScaI AGT'ACT
Cuts at: 3650 3650
Size: 4133
SfcI C'TryA_G
Cuts at: 33 932 1176 1861 2854 3045 33
Size: 899 244 685 993 191 1121
Fragments arranged by size:
1121 993 899 685 244 191
SfoI GGC'GCC
Cuts at: 1565 1813 1565
Size: 248 3885
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
SmaI CCC'GGG
Cuts at: 1155 1155
Size: 4133
SmlI C'TyrA_G
Cuts at: 329 1288 2203 2695 2957 329
Size: 959 915 492 262 1505
Fragments arranged by size:
1505 959 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4133
SphI G_CATG'C
Cuts at: 382 1186 1686 382
Size: 804 500 2829
Fragments arranged by size:
2829 804 500
StyI C'CwwG_G
Cuts at: 1267 1621 1267
Size: 354 3779
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 393 1716 1849 2308 2517 393
Size: 1323 133 459 209 2009
Fragments arranged by size:
2009 1323 459 209 133
TatI w'GTAC_w
Cuts at: 3648 3648
Size: 4133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3650 3682 3650
Size: 32 4101
Tth111I GACn'n_nGTC
Cuts at: 1930 1930
Size: 4133
XbaI T'CTAG_A
Cuts at: 1164 1164
Size: 4133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1628 114
Size: 1514 2619
XmaI C'CCGG_G
Cuts at: 1153 1153
Size: 4133
XmnI GAAnn'nnTTC
Cuts at: 927 927
Size: 4133
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BsaXI BseRI BsiWI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI