Restriction map for
pF1KB6425
(Circular) (Six-base) MAPSORT of: pF1KB6425.seq Check: 1817 from: 1 to: 4043
pF1KB6425 4043 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 244 244
Size: 4043
AccI GT'mk_AC
Cuts at: 1081 1081
Size: 4043
Acc65I G'GTAC_C
Cuts at: 1059 1059
Size: 4043
AclI AA'CG_TT
Cuts at: 3883 3883
Size: 4043
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 1868 2300 3047 3315 221
Size: 1647 432 747 268 949
Fragments arranged by size:
1647 949 747 432 268
AflIII A'CryG_T
Cuts at: 1395 2499 3476 1395
Size: 1104 977 1962
Fragments arranged by size:
1962 1104 977
AgeI A'CCGG_T
Cuts at: 2431 2431
Size: 4043
AlwNI CAG_nnn'CTG
Cuts at: 318 1348 2915 318
Size: 1030 1567 1446
Fragments arranged by size:
1567 1446 1030
ApaI G_GGCC'C
Cuts at: 404 404
Size: 4043
ApaLI G'TGCA_C
Cuts at: 2813 2813
Size: 4043
ApoI r'AATT_y
Cuts at: 1027 1047 3396 3652 1027
Size: 20 2349 256 1418
Fragments arranged by size:
2349 1418 256 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 4043
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
AvaI C'yCGr_G
Cuts at: 455 753 1063 455
Size: 298 310 3435
Fragments arranged by size:
3435 310 298
BamHI G'GATC_C
Cuts at: 1068 1068
Size: 4043
BanI G'GyrC_C
Cuts at: 1059 1473 1721 1756 1059
Size: 414 248 35 3346
Fragments arranged by size:
3346 414 248 35
BanII G_rGCy'C
Cuts at: 404 551 724 899 1057 2087 404
Size: 147 173 175 158 1030 2360
Fragments arranged by size:
2360 1030 175 173 158 147
BbeI G_GCGC'C
Cuts at: 1477 1725 1477
Size: 248 3795
BbvCI CC'TCA_GC
Cuts at: 683 683
Size: 4043
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 661 695 661
Size: 34 4009
BciVI GTATCCnnnnn_n'
Cuts at: 1941 2702 4043 1941
Size: 761 1341 1941
Fragments arranged by size:
1941 1341 761
BclI T'GATC_A
Cuts at: 1563 1563
Size: 4043
BglI GCCn_nnn'nGGC
Cuts at: 538 829 538
Size: 291 3752
BglII A'GATC_T
Cuts at: 1558 3595 1558
Size: 2037 2006
BlpI GC'TnA_GC
Cuts at: 151 1155 151
Size: 1004 3039
Bme1580I G_kGCm'C
Cuts at: 404 1668 1761 2817 404
Size: 1264 93 1056 1630
Fragments arranged by size:
1630 1264 1056 93
BmgBI CAC'GTC
Cuts at: 335 335
Size: 4043
BmrI ACTGGGnnnn_n'
Cuts at: 802 1255 1336 1671 3815 802
Size: 453 81 335 2144 1030
Fragments arranged by size:
2144 1030 453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 413 681 323
Size: 90 268 3685
Fragments arranged by size:
3685 268 90
Bpu10I CC'TnA_GC
Cuts at: 246 683 246
Size: 437 3606
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 2098 2590 2888 1219
Size: 879 492 298 2374
Fragments arranged by size:
2374 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 481 589 3747 481
Size: 108 3158 777
Fragments arranged by size:
3158 777 108
BsaAI yAC'GTr
Cuts at: 1396 1396
Size: 4043
BsaBI GATnn'nnATC
Cuts at: 6 1557 3594 6
Size: 1551 2037 455
Fragments arranged by size:
2037 1551 455
BsaHI Gr'CG_yC
Cuts at: 1474 1722 2424 3920 1474
Size: 248 702 1496 1597
Fragments arranged by size:
1597 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1232 1753 2431 2705 2852 3263 1232
Size: 521 678 274 147 411 2012
Fragments arranged by size:
2012 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 223 253 404 434 827 857 875 905 223
Size: 30 151 30 393 30 18 30 3361
Fragments arranged by size:
3361 393 151 30 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 988 988
Size: 4043
BseYI C'CCAG_C
Cuts at: 616 708 864 1468 2226 2803 3299 616
Size: 92 156 604 758 577 496 1360
Fragments arranged by size:
1360 758 604 577 496 156 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 272
Size: 4043
BsiEI CG_ry'CG
Cuts at: 104 1585 1631 2839 104
Size: 1481 46 1208 1308
Fragments arranged by size:
1481 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1057 1835 2025 2817 1057
Size: 778 190 792 2283
Fragments arranged by size:
2283 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 341 341
Size: 4043
Bsp1286I G_dGCh'C
Cuts at: 404 551 724 899 1057 1668 1761 1835 2025
Size: 147 173 175 158 611 93 74 190
Cuts at: 2025 2087 2817 404
Size: 62 730 1630
Fragments arranged by size:
1630 730 611 190 175 173 158 147
93 74 62
BspEI T'CCGG_A
Cuts at: 3263 3263
Size: 4043
BspHI T'CATG_A
Cuts at: 3219 3219
Size: 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 244 1093 1307 1609 1990 244
Size: 849 214 302 381 2297
Fragments arranged by size:
2297 849 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2335 2389 3873 4038 50
Size: 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 875 1955 875
Size: 1080 2963
BsrFI r'CCGG_y
Cuts at: 162 349 387 2041 2222 2431 162
Size: 187 38 1654 181 209 1774
Fragments arranged by size:
1774 1654 209 187 181 38
BssHII G'CGCG_C
Cuts at: 114 140 114
Size: 26 4017
BssSI C'ACGA_G
Cuts at: 889 2314 2672 889
Size: 1425 358 2260
Fragments arranged by size:
2260 1425 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1420 1420
Size: 4043
BstBI TT'CG_AA
Cuts at: 2404 2404
Size: 4043
BstXI CCAn_nnnn'nTGG
Cuts at: 354 354
Size: 4043
BstYI r'GATC_y
Cuts at: 1 1068 1535 1558 1893 2139 3151 3237 3249
Size: 1067 467 23 335 246 1012 86 12
Cuts at: 3249 3595 1
Size: 346 449
Fragments arranged by size:
1067 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 536 779 824 536
Size: 243 45 3755
Fragments arranged by size:
3755 243 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1993 2174 2337 1993
Size: 181 163 3699
Fragments arranged by size:
3699 181 163
BtsI GCAGTG_nn'
Cuts at: 1286 1286
Size: 4043
DraI TTT'AAA
Cuts at: 1042 1042
Size: 4043
DrdI GACnn_nn'nnGTC
Cuts at: 1749 2607 1749
Size: 858 3185
EaeI y'GGCC_r
Cuts at: 585 776 1628 1802 2193 2220 3565 585
Size: 191 852 174 391 27 1345 1063
Fragments arranged by size:
1345 1063 852 391 191 174 27
EagI C'GGCC_G
Cuts at: 1628 1628
Size: 4043
EarI CTCTTCn'nnn_
Cuts at: 2066 2276 2066
Size: 210 3833
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2251 2561 2707 3853 2251
Size: 310 146 1146 2441
Fragments arranged by size:
2441 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1055 1055
Size: 4043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 323 413 681 1868 2300 3047 3315 221
Size: 102 90 268 1187 432 747 268 949
Fragments arranged by size:
1187 949 747 432 268 268 102 90
EcoO109I rG'GnC_Cy
Cuts at: 400 613 658 830 840 998 1182 400
Size: 213 45 172 10 158 184 3261
Fragments arranged by size:
3261 213 184 172 158 45 10
EcoRI G'AATT_C
Cuts at: 1027 1047 1027
Size: 20 4023
EcoRV GAT'ATC
Cuts at: 270 270
Size: 4043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 646 2062 646
Size: 1416 2627
FspI TGC'GCA
Cuts at: 1824 1824
Size: 4043
HaeII r_GCGC'y
Cuts at: 124 151 1477 1725 2747 3726 124
Size: 27 1326 248 1022 979 441
Fragments arranged by size:
1326 1022 979 441 248 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 495 527 679 711 1885 1915 1917
Size: 32 240 32 152 32 1174 30 2
Cuts at: 1917 1947 223
Size: 30 2319
Fragments arranged by size:
2319 1174 240 152 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1082 1082
Size: 4043
KasI G'GCGC_C
Cuts at: 1473 1721 1473
Size: 248 3795
KpnI G_GTAC'C
Cuts at: 1063 1063
Size: 4043
MluI A'CGCG_T
Cuts at: 3476 3476
Size: 4043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2714 2898 2714
Size: 184 3859
MscI TGG'CCA
Cuts at: 587 1804 587
Size: 1217 2826
MslI CAynn'nnrTG
Cuts at: 2032 2159 2032
Size: 127 3916
MspA1I CmG'CkG
Cuts at: 367 1154 1468 1828 2841 3086 367
Size: 787 314 360 1013 245 1324
Fragments arranged by size:
1324 1013 787 360 314 245
NaeI GCC'GGC
Cuts at: 164 389 2224 164
Size: 225 1835 1983
Fragments arranged by size:
1983 1835 225
NarI GG'CG_CC
Cuts at: 1474 1722 1474
Size: 248 3795
NgoMIV G'CCGG_C
Cuts at: 162 387 2222 162
Size: 225 1835 1983
Fragments arranged by size:
1983 1835 225
NspI r_CATG'y
Cuts at: 579 1096 1596 2503 579
Size: 517 500 907 2119
Fragments arranged by size:
2119 907 517 500
PasI CC'CwG_GG
Cuts at: 482 482
Size: 4043
PciI A'CATG_T
Cuts at: 2499 2499
Size: 4043
PfoI T'CCnGG_A
Cuts at: 845 845
Size: 4043
PmeI GTTT'AAAC
Cuts at: 1042 1042
Size: 4043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 4011
PpuMI rG'GwC_Cy
Cuts at: 613 658 613
Size: 45 3998
PspOMI G'GGCC_C
Cuts at: 400 400
Size: 4043
PstI C_TGCA'G
Cuts at: 161 612 1090 1775 161
Size: 451 478 685 2429
Fragments arranged by size:
2429 685 478 451
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4043
PvuII CAG'CTG
Cuts at: 367 1468 1828 367
Size: 1101 360 2582
Fragments arranged by size:
2582 1101 360
RsrII CG'GwC_CG
Cuts at: 2238 2238
Size: 4043
SacI G_AGCT'C
Cuts at: 1057 1057
Size: 4043
SalI G'TCGA_C
Cuts at: 1080 1080
Size: 4043
SapI GCTCTTCn'nnn_
Cuts at: 2066 2276 2066
Size: 210 3833
SbfI CC_TGCA'GG
Cuts at: 612 1090 612
Size: 478 3565
ScaI AGT'ACT
Cuts at: 3560 3560
Size: 4043
SfcI C'TryA_G
Cuts at: 33 157 362 419 608 770 1086 1771 2764
Size: 124 205 57 189 162 316 685 993
Cuts at: 2764 2955 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 316 205 191 189 162
124 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 829 829
Size: 4043
SfoI GGC'GCC
Cuts at: 1475 1723 1475
Size: 248 3795
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
SmaI CCC'GGG
Cuts at: 457 1065 457
Size: 608 3435
SmlI C'TyrA_G
Cuts at: 1198 2113 2605 2867 1198
Size: 915 492 262 2374
Fragments arranged by size:
2374 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4043
SphI G_CATG'C
Cuts at: 1096 1596 1096
Size: 500 3543
StuI AGG'CCT
Cuts at: 517 887 517
Size: 370 3673
StyI C'CwwG_G
Cuts at: 404 968 1177 1531 404
Size: 564 209 354 2916
Fragments arranged by size:
2916 564 354 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1626 1759 2218 2427 1626
Size: 133 459 209 3242
Fragments arranged by size:
3242 459 209 133
TatI w'GTAC_w
Cuts at: 3558 3558
Size: 4043
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 827 859 875 907 3560 3592 827
Size: 32 16 32 2653 32 1278
Fragments arranged by size:
2653 1278 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 1840 1840
Size: 4043
XbaI T'CTAG_A
Cuts at: 1074 1074
Size: 4043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 649 1538 649
Size: 889 3154
XmaI C'CCGG_G
Cuts at: 455 1063 455
Size: 608 3435
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BmtI BsiWI BsmI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI