Restriction map for
pF1KB6427
(Circular) (Six-base) MAPSORT of: pF1KB6427.seq Check: 9514 from: 1 to: 4031
pF1KB6427 4031 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 963 963
Size: 4031
AccI GT'mk_AC
Cuts at: 1069 1069
Size: 4031
Acc65I G'GTAC_C
Cuts at: 1047 1047
Size: 4031
AclI AA'CG_TT
Cuts at: 3871 3871
Size: 4031
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 400 562 1856 2288 3035 3303 267
Size: 133 162 1294 432 747 268 995
Fragments arranged by size:
1294 995 747 432 268 162 133
AflIII A'CryG_T
Cuts at: 1383 2487 3464 1383
Size: 1104 977 1950
Fragments arranged by size:
1950 1104 977
AgeI A'CCGG_T
Cuts at: 2419 2419
Size: 4031
AlwNI CAG_nnn'CTG
Cuts at: 138 252 507 1336 2903 138
Size: 114 255 829 1567 1266
Fragments arranged by size:
1567 1266 829 255 114
ApaLI G'TGCA_C
Cuts at: 350 2801 350
Size: 2451 1580
ApoI r'AATT_y
Cuts at: 757 1035 3384 3640 757
Size: 278 2349 256 1148
Fragments arranged by size:
2349 1148 278 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4031
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
AvaI C'yCGr_G
Cuts at: 405 1051 405
Size: 646 3385
AvrII C'CTAG_G
Cuts at: 474 474
Size: 4031
BamHI G'GATC_C
Cuts at: 1056 1056
Size: 4031
BanI G'GyrC_C
Cuts at: 1047 1461 1709 1744 1047
Size: 414 248 35 3334
Fragments arranged by size:
3334 414 248 35
BanII G_rGCy'C
Cuts at: 145 590 1045 2075 145
Size: 445 455 1030 2101
Fragments arranged by size:
2101 1030 455 445
BbeI G_GCGC'C
Cuts at: 1465 1713 1465
Size: 248 3783
BbsI GAAGACnn'nnnn_
Cuts at: 926 926
Size: 4031
BbvCI CC'TCA_GC
Cuts at: 689 689
Size: 4031
BciVI GTATCCnnnnn_n'
Cuts at: 1929 2690 4031 1929
Size: 761 1341 1929
Fragments arranged by size:
1929 1341 761
BclI T'GATC_A
Cuts at: 1551 1551
Size: 4031
BglII A'GATC_T
Cuts at: 1546 3583 1546
Size: 2037 1994
BlpI GC'TnA_GC
Cuts at: 139 1143 139
Size: 1004 3027
Bme1580I G_kGCm'C
Cuts at: 354 902 1656 1749 2805 354
Size: 548 754 93 1056 1580
Fragments arranged by size:
1580 1056 754 548 93
BmrI ACTGGGnnnn_n'
Cuts at: 347 642 876 1243 1324 1659 3803 347
Size: 295 234 367 81 335 2144 575
Fragments arranged by size:
2144 575 367 335 295 234 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 733 508
Size: 225 3806
Bpu10I CC'TnA_GC
Cuts at: 298 689 298
Size: 391 3640
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 2086 2578 2876 1207
Size: 879 492 298 2362
Fragments arranged by size:
2362 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 805 3735 805
Size: 2930 1101
BsaAI yAC'GTr
Cuts at: 1384 1384
Size: 4031
BsaBI GATnn'nnATC
Cuts at: 6 1545 3582 6
Size: 1539 2037 455
Fragments arranged by size:
2037 1539 455
BsaHI Gr'CG_yC
Cuts at: 1462 1710 2412 3908 1462
Size: 248 702 1496 1585
Fragments arranged by size:
1585 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1220 1741 2419 2693 2840 3251 1220
Size: 521 678 274 147 411 2000
Fragments arranged by size:
2000 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 437 467 437
Size: 30 4001
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 393 580 763 393
Size: 187 183 3661
Fragments arranged by size:
3661 187 183
BseYI C'CCAG_C
Cuts at: 245 906 983 1456 2214 2791 3287 245
Size: 661 77 473 758 577 496 989
Fragments arranged by size:
989 758 661 577 496 473 77
BsiEI CG_ry'CG
Cuts at: 104 1573 1619 2827 104
Size: 1469 46 1208 1308
Fragments arranged by size:
1469 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 145 354 412 590 644 1045 1823 2013 2805
Size: 209 58 178 54 401 778 190 792
Cuts at: 2805 145
Size: 1371
Fragments arranged by size:
1371 792 778 401 209 190 178 58
54
BsmI GAATG_Cn'
Cuts at: 1011 1011
Size: 4031
BsmBI CGTCTCn'nnnn_
Cuts at: 268 268
Size: 4031
Bsp1286I G_dGCh'C
Cuts at: 145 354 412 590 644 902 1045 1656 1749
Size: 209 58 178 54 258 143 611 93
Cuts at: 1749 1823 2013 2075 2805 145
Size: 74 190 62 730 1371
Fragments arranged by size:
1371 730 611 258 209 190 178 143
93 74 62 58 54
BspEI T'CCGG_A
Cuts at: 3251 3251
Size: 4031
BspHI T'CATG_A
Cuts at: 3207 3207
Size: 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 569 625 963 1081 1295 1597 1978 569
Size: 56 338 118 214 302 381 2622
Fragments arranged by size:
2622 381 338 302 214 118 56
BsrBI CCG'CTC
Cuts at: 50 2323 2377 3861 4026 50
Size: 2273 54 1484 165 55
Fragments arranged by size:
2273 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1943 1943
Size: 4031
BsrFI r'CCGG_y
Cuts at: 1012 2029 2210 2419 1012
Size: 1017 181 209 2624
Fragments arranged by size:
2624 1017 209 181
BsrGI T'GTAC_A
Cuts at: 765 765
Size: 4031
BssSI C'ACGA_G
Cuts at: 901 2302 2660 901
Size: 1401 358 2272
Fragments arranged by size:
2272 1401 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1408 1408
Size: 4031
BstBI TT'CG_AA
Cuts at: 2392 2392
Size: 4031
BstXI CCAn_nnnn'nTGG
Cuts at: 890 943 890
Size: 53 3978
BstYI r'GATC_y
Cuts at: 1 1056 1523 1546 1881 2127 3139 3225 3237
Size: 1055 467 23 335 246 1012 86 12
Cuts at: 3237 3583 1
Size: 346 449
Fragments arranged by size:
1055 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 272 441 554 107
Size: 165 169 113 3584
Fragments arranged by size:
3584 169 165 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1981 2162 2325 1981
Size: 181 163 3687
Fragments arranged by size:
3687 181 163
BtsI GCAGTG_nn'
Cuts at: 1274 1274
Size: 4031
DraI TTT'AAA
Cuts at: 1030 1030
Size: 4031
DraIII CAC_nnn'GTG
Cuts at: 729 729
Size: 4031
DrdI GACnn_nn'nnGTC
Cuts at: 1737 2595 1737
Size: 858 3173
EaeI y'GGCC_r
Cuts at: 110 945 1022 1616 1790 2181 2208 3553 110
Size: 835 77 594 174 391 27 1345 588
Fragments arranged by size:
1345 835 594 588 391 174 77 27
EagI C'GGCC_G
Cuts at: 1616 1616
Size: 4031
EarI CTCTTCn'nnn_
Cuts at: 538 2054 2264 538
Size: 1516 210 2305
Fragments arranged by size:
2305 1516 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 709 1017 2239 2549 2695 3841 709
Size: 308 1222 310 146 1146 899
Fragments arranged by size:
1222 1146 899 310 308 146
EcoICRI GAG'CTC
Cuts at: 143 588 1043 143
Size: 445 455 3131
Fragments arranged by size:
3131 455 445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 400 508 562 733 1856 2288 3035 3303
Size: 133 108 54 171 1123 432 747 268
Cuts at: 3303 267
Size: 995
Fragments arranged by size:
1123 995 747 432 268 171 133 108
54
EcoNI CCTnn'n_nnAGG
Cuts at: 418 418
Size: 4031
EcoO109I rG'GnC_Cy
Cuts at: 341 504 614 1170 341
Size: 163 110 556 3202
Fragments arranged by size:
3202 556 163 110
EcoRI G'AATT_C
Cuts at: 1035 1035
Size: 4031
EcoRV GAT'ATC
Cuts at: 129 129
Size: 4031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 4031
FspI TGC'GCA
Cuts at: 1812 1812
Size: 4031
HaeII r_GCGC'y
Cuts at: 1465 1713 2735 3714 1465
Size: 248 1022 979 1782
Fragments arranged by size:
1782 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 201 233 1873 1903 1905 1935 201
Size: 32 1640 30 2 30 2297
Fragments arranged by size:
2297 1640 32 30 30 2
HincII GTy'rAC
Cuts at: 630 1070 630
Size: 440 3591
HindIII A'AGCT_T
Cuts at: 386 386
Size: 4031
KasI G'GCGC_C
Cuts at: 1461 1709 1461
Size: 248 3783
KpnI G_GTAC'C
Cuts at: 1051 1051
Size: 4031
MluI A'CGCG_T
Cuts at: 3464 3464
Size: 4031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2702 2886 2702
Size: 184 3847
MscI TGG'CCA
Cuts at: 112 1792 112
Size: 1680 2351
MslI CAynn'nnrTG
Cuts at: 173 331 2020 2147 173
Size: 158 1689 127 2057
Fragments arranged by size:
2057 1689 158 127
MspA1I CmG'CkG
Cuts at: 138 357 653 711 950 983 1142 1456 1816
Size: 219 296 58 239 33 159 314 360
Cuts at: 1816 2829 3074 138
Size: 1013 245 1095
Fragments arranged by size:
1095 1013 360 314 296 245 239 219
159 58 33
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 4031
NarI GG'CG_CC
Cuts at: 1462 1710 1462
Size: 248 3783
NcoI C'CATG_G
Cuts at: 107 272 107
Size: 165 3866
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 4031
NspI r_CATG'y
Cuts at: 1084 1584 2491 1084
Size: 500 907 2624
Fragments arranged by size:
2624 907 500
PciI A'CATG_T
Cuts at: 2487 2487
Size: 4031
PflMI CCAn_nnn'nTGG
Cuts at: 252 252
Size: 4031
PfoI T'CCnGG_A
Cuts at: 673 673
Size: 4031
PmeI GTTT'AAAC
Cuts at: 1030 1030
Size: 4031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3999
PpuMI rG'GwC_Cy
Cuts at: 341 504 614 341
Size: 163 110 3758
Fragments arranged by size:
3758 163 110
PshAI GACnn'nnGTC
Cuts at: 421 421
Size: 4031
PspXI vC'TCGA_Gb
Cuts at: 405 405
Size: 4031
PstI C_TGCA'G
Cuts at: 974 1078 1763 974
Size: 104 685 3242
Fragments arranged by size:
3242 685 104
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4031
PvuII CAG'CTG
Cuts at: 138 357 653 711 1456 1816 138
Size: 219 296 58 745 360 2353
Fragments arranged by size:
2353 745 360 296 219 58
RsrII CG'GwC_CG
Cuts at: 2226 2226
Size: 4031
SacI G_AGCT'C
Cuts at: 145 590 1045 145
Size: 445 455 3131
Fragments arranged by size:
3131 455 445
SalI G'TCGA_C
Cuts at: 1068 1068
Size: 4031
SanDI GG'GwC_CC
Cuts at: 341 341
Size: 4031
SapI GCTCTTCn'nnn_
Cuts at: 538 2054 2264 538
Size: 1516 210 2305
Fragments arranged by size:
2305 1516 210
SbfI CC_TGCA'GG
Cuts at: 974 1078 974
Size: 104 3927
ScaI AGT'ACT
Cuts at: 703 3548 703
Size: 2845 1186
SexAI A'CCwGG_T
Cuts at: 725 725
Size: 4031
SfcI C'TryA_G
Cuts at: 33 846 970 1074 1759 2752 2943 33
Size: 813 124 104 685 993 191 1121
Fragments arranged by size:
1121 993 813 685 191 124 104
SfoI GGC'GCC
Cuts at: 1463 1711 1463
Size: 248 3783
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
SgrAI Cr'CCGG_yG
Cuts at: 1012 1012
Size: 4031
SmaI CCC'GGG
Cuts at: 1053 1053
Size: 4031
SmlI C'TyrA_G
Cuts at: 405 1186 2101 2593 2855 405
Size: 781 915 492 262 1581
Fragments arranged by size:
1581 915 781 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4031
SphI G_CATG'C
Cuts at: 1084 1584 1084
Size: 500 3531
StuI AGG'CCT
Cuts at: 682 682
Size: 4031
StyI C'CwwG_G
Cuts at: 107 113 272 474 645 1165 1519 107
Size: 6 159 202 171 520 354 2619
Fragments arranged by size:
2619 520 354 202 171 159 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 445 454 624 819 898 975 1614 1747 2206
Size: 9 170 195 79 77 639 133 459
Cuts at: 2206 2415 445
Size: 209 2061
Fragments arranged by size:
2061 639 459 209 195 170 133 79
77 9
TatI w'GTAC_w
Cuts at: 701 765 3546 701
Size: 64 2781 1186
Fragments arranged by size:
2781 1186 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 437 469 3548 3580 437
Size: 32 3079 32 888
Fragments arranged by size:
3079 888 32 32
Tth111I GACn'n_nGTC
Cuts at: 339 1828 339
Size: 1489 2542
XbaI T'CTAG_A
Cuts at: 1062 1062
Size: 4031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1017 1526 1017
Size: 509 3522
XhoI C'TCGA_G
Cuts at: 405 405
Size: 4031
XmaI C'CCGG_G
Cuts at: 1051 1051
Size: 4031
XmnI GAAnn'nnTTC
Cuts at: 547 781 547
Size: 234 3797
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BssHII BstEII BstZ17I Bsu36I ClaI CspCI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PsiI PspOMI PsrI SacII SfiI
SnaBI SrfI SspI SwaI ZraI