Restriction map for
pF1KB6428
(Circular) (Six-base) MAPSORT of: pF1KB6428.seq Check: 9694 from: 1 to: 4082
pF1KB6428 4082 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 1120 1120
Size: 4082
Acc65I G'GTAC_C
Cuts at: 1098 1098
Size: 4082
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1907 2339 3086 3354 1907
Size: 432 747 268 2635
Fragments arranged by size:
2635 747 432 268
AfeI AGC'GCT
Cuts at: 920 920
Size: 4082
AflIII A'CryG_T
Cuts at: 1434 2538 3515 1434
Size: 1104 977 2001
Fragments arranged by size:
2001 1104 977
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 872 872
Size: 4082
AlwNI CAG_nnn'CTG
Cuts at: 1387 2954 1387
Size: 1567 2515
ApaI G_GGCC'C
Cuts at: 503 503
Size: 4082
ApaLI G'TGCA_C
Cuts at: 2852 2852
Size: 4082
ApoI r'AATT_y
Cuts at: 172 640 722 957 1086 3435 3691 172
Size: 468 82 235 129 2349 256 563
Fragments arranged by size:
2349 563 468 256 235 129 82
AscI GG'CGCG_CC
Cuts at: 316 316
Size: 4082
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 1102 1102
Size: 4082
BamHI G'GATC_C
Cuts at: 1107 1107
Size: 4082
BanI G'GyrC_C
Cuts at: 1098 1512 1760 1795 1098
Size: 414 248 35 3385
Fragments arranged by size:
3385 414 248 35
BanII G_rGCy'C
Cuts at: 503 1096 2126 503
Size: 593 1030 2459
Fragments arranged by size:
2459 1030 593
BbeI G_GCGC'C
Cuts at: 1516 1764 1516
Size: 248 3834
BbsI GAAGACnn'nnnn_
Cuts at: 546 797 546
Size: 251 3831
BbvCI CC'TCA_GC
Cuts at: 348 348
Size: 4082
BciVI GTATCCnnnnn_n'
Cuts at: 1980 2741 4082 1980
Size: 761 1341 1980
Fragments arranged by size:
1980 1341 761
BclI T'GATC_A
Cuts at: 263 1602 263
Size: 1339 2743
BglII A'GATC_T
Cuts at: 711 1597 3634 711
Size: 886 2037 1159
Fragments arranged by size:
2037 1159 886
BlpI GC'TnA_GC
Cuts at: 1194 1194
Size: 4082
Bme1580I G_kGCm'C
Cuts at: 503 1707 1800 2856 503
Size: 1204 93 1056 1729
Fragments arranged by size:
1729 1204 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1294 1375 1710 3854 1294
Size: 81 335 2144 1522
Fragments arranged by size:
2144 1522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 989
Size: 4082
Bpu10I CC'TnA_GC
Cuts at: 348 348
Size: 4082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 1258 2137 2629 2927 686
Size: 572 879 492 298 1841
Fragments arranged by size:
1841 879 572 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3786 3786
Size: 4082
BsaAI yAC'GTr
Cuts at: 225 560 1065 1435 225
Size: 335 505 370 2872
Fragments arranged by size:
2872 505 370 335
BsaBI GATnn'nnATC
Cuts at: 6 1596 3633 6
Size: 1590 2037 455
Fragments arranged by size:
2037 1590 455
BsaHI Gr'CG_yC
Cuts at: 1513 1761 2463 3959 1513
Size: 248 702 1496 1636
Fragments arranged by size:
1636 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1271 1792 2470 2744 2891 3302 1271
Size: 521 678 274 147 411 2051
Fragments arranged by size:
2051 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 308 308
Size: 4082
BseYI C'CCAG_C
Cuts at: 1507 2265 2842 3338 1507
Size: 758 577 496 2251
Fragments arranged by size:
2251 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 842 326
Size: 516 3566
BsiEI CG_ry'CG
Cuts at: 104 1624 1670 2878 104
Size: 1520 46 1208 1308
Fragments arranged by size:
1520 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1096 1874 2064 2856 1096
Size: 778 190 792 2322
Fragments arranged by size:
2322 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 503 1096 1707 1800 1874 2064 2126 2856 503
Size: 593 611 93 74 190 62 730 1729
Fragments arranged by size:
1729 730 611 593 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3302 3302
Size: 4082
BspHI T'CATG_A
Cuts at: 3258 3258
Size: 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1132 1346 1648 2029 1132
Size: 214 302 381 3185
Fragments arranged by size:
3185 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2374 2428 3912 4077 50
Size: 2324 54 1484 165 55
Fragments arranged by size:
2324 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1994 1994
Size: 4082
BsrFI r'CCGG_y
Cuts at: 2080 2261 2470 2080
Size: 181 209 3692
Fragments arranged by size:
3692 209 181
BssHII G'CGCG_C
Cuts at: 316 316
Size: 4082
BssSI C'ACGA_G
Cuts at: 2353 2711 2353
Size: 358 3724
BstAPI GCAn_nnn'nTGC
Cuts at: 1459 1459
Size: 4082
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstYI r'GATC_y
Cuts at: 1 711 1107 1574 1597 1932 2178 3190 3276
Size: 710 396 467 23 335 246 1012 86
Cuts at: 3276 3288 3634 1
Size: 12 346 449
Fragments arranged by size:
1012 710 467 449 396 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 691 691
Size: 4082
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2032 2213 2376 2032
Size: 181 163 3738
Fragments arranged by size:
3738 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1325
Size: 4082
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 1667 1841 2232 2259 3604 1667
Size: 174 391 27 1345 2145
Fragments arranged by size:
2145 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1667 1667
Size: 4082
EarI CTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 473 2290 2600 2746 3892 473
Size: 1817 310 146 1146 663
Fragments arranged by size:
1817 1146 663 310 146
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 1907 2339 3086 3354 989
Size: 918 432 747 268 1717
Fragments arranged by size:
1717 918 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 507 507
Size: 4082
EcoO109I rG'GnC_Cy
Cuts at: 500 1221 500
Size: 721 3361
EcoRI G'AATT_C
Cuts at: 172 1086 172
Size: 914 3168
EcoRV GAT'ATC
Cuts at: 657 657
Size: 4082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1045 2101 1045
Size: 1056 3026
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 922 1516 1764 2786 3765 922
Size: 594 248 1022 979 1239
Fragments arranged by size:
1239 1022 979 594 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 752 784 962 994 1924 1954 1956 1986 752
Size: 32 178 32 930 30 2 30 2848
Fragments arranged by size:
2848 930 178 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1121 1121
Size: 4082
HindIII A'AGCT_T
Cuts at: 443 443
Size: 4082
KasI G'GCGC_C
Cuts at: 1512 1760 1512
Size: 248 3834
KpnI G_GTAC'C
Cuts at: 1102 1102
Size: 4082
MluI A'CGCG_T
Cuts at: 3515 3515
Size: 4082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2753 2937 2753
Size: 184 3898
MscI TGG'CCA
Cuts at: 1843 1843
Size: 4082
MslI CAynn'nnrTG
Cuts at: 910 2071 2198 910
Size: 1161 127 2794
Fragments arranged by size:
2794 1161 127
MspA1I CmG'CkG
Cuts at: 302 547 693 870 1193 1507 1867 2880 3125
Size: 245 146 177 323 314 360 1013 245
Cuts at: 3125 302
Size: 1259
Fragments arranged by size:
1259 1013 360 323 314 245 245 177
146
NaeI GCC'GGC
Cuts at: 2263 2263
Size: 4082
NarI GG'CG_CC
Cuts at: 1513 1761 1513
Size: 248 3834
NgoMIV G'CCGG_C
Cuts at: 2261 2261
Size: 4082
NspI r_CATG'y
Cuts at: 1135 1635 2542 1135
Size: 500 907 2675
Fragments arranged by size:
2675 907 500
PciI A'CATG_T
Cuts at: 2538 2538
Size: 4082
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 865 897 3221 3253 865
Size: 32 2324 32 1694
Fragments arranged by size:
2324 1694 32 32
PspOMI G'GGCC_C
Cuts at: 499 499
Size: 4082
PstI C_TGCA'G
Cuts at: 1129 1814 1129
Size: 685 3397
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 870 1507 1867 870
Size: 637 360 3085
Fragments arranged by size:
3085 637 360
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SacII CC_GC'GG
Cuts at: 694 694
Size: 4082
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
SbfI CC_TGCA'GG
Cuts at: 1129 1129
Size: 4082
ScaI AGT'ACT
Cuts at: 3599 3599
Size: 4082
SfcI C'TryA_G
Cuts at: 33 437 1125 1810 2803 2994 33
Size: 404 688 685 993 191 1121
Fragments arranged by size:
1121 993 688 685 404 191
SfoI GGC'GCC
Cuts at: 1514 1762 1514
Size: 248 3834
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 1104 1104
Size: 4082
SmlI C'TyrA_G
Cuts at: 701 1237 2152 2644 2906 701
Size: 536 915 492 262 1877
Fragments arranged by size:
1877 915 536 492 262
SnaBI TAC'GTA
Cuts at: 1065 1065
Size: 4082
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
SspI AAT'ATT
Cuts at: 164 164
Size: 4082
StuI AGG'CCT
Cuts at: 245 245
Size: 4082
StyI C'CwwG_G
Cuts at: 369 503 1216 1570 369
Size: 134 713 354 2881
Fragments arranged by size:
2881 713 354 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1665 1798 2257 2466 1665
Size: 133 459 209 3281
Fragments arranged by size:
3281 459 209 133
TatI w'GTAC_w
Cuts at: 3597 3597
Size: 4082
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4050
Tth111I GACn'n_nGTC
Cuts at: 1879 1879
Size: 4082
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 332 874 1577 332
Size: 542 703 2837
Fragments arranged by size:
2837 703 542
XmaI C'CCGG_G
Cuts at: 1102 1102
Size: 4082
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SrfI SwaI XhoI XmnI ZraI