Restriction map for
pF1KB6430
(Circular) (Six-base) MAPSORT of: pF1KB6430.seq Check: 9212 from: 1 to: 4142
pF1KB6430 4142 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1180 1180
Size: 4142
Acc65I G'GTAC_C
Cuts at: 1158 1158
Size: 4142
AclI AA'CG_TT
Cuts at: 3982 3982
Size: 4142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1967 2399 3146 3414 1967
Size: 432 747 268 2695
Fragments arranged by size:
2695 747 432 268
AflIII A'CryG_T
Cuts at: 756 965 1494 2598 3575 756
Size: 209 529 1104 977 1323
Fragments arranged by size:
1323 1104 977 529 209
AgeI A'CCGG_T
Cuts at: 2530 2530
Size: 4142
AhdI GACnn_n'nnGTC
Cuts at: 504 504
Size: 4142
AleI CACnn'nnGTG
Cuts at: 685 685
Size: 4142
AlwNI CAG_nnn'CTG
Cuts at: 435 1447 3014 435
Size: 1012 1567 1563
Fragments arranged by size:
1567 1563 1012
ApaI G_GGCC'C
Cuts at: 171 810 171
Size: 639 3503
ApaLI G'TGCA_C
Cuts at: 2912 2912
Size: 4142
ApoI r'AATT_y
Cuts at: 1146 3495 3751 1146
Size: 2349 256 1537
Fragments arranged by size:
2349 1537 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
AvaI C'yCGr_G
Cuts at: 162 1162 162
Size: 1000 3142
BamHI G'GATC_C
Cuts at: 1167 1167
Size: 4142
BanI G'GyrC_C
Cuts at: 135 339 939 1158 1572 1820 1855 135
Size: 204 600 219 414 248 35 2422
Fragments arranged by size:
2422 600 414 248 219 204 35
BanII G_rGCy'C
Cuts at: 171 199 254 810 884 1001 1132 1156 2186
Size: 28 55 556 74 117 131 24 1030
Cuts at: 2186 171
Size: 2127
Fragments arranged by size:
2127 1030 556 131 117 74 55 28
24
BbeI G_GCGC'C
Cuts at: 139 343 1576 1824 139
Size: 204 1233 248 2457
Fragments arranged by size:
2457 1233 248 204
BbsI GAAGACnn'nnnn_
Cuts at: 118 1016 118
Size: 898 3244
BciVI GTATCCnnnnn_n'
Cuts at: 2040 2801 4142 2040
Size: 761 1341 2040
Fragments arranged by size:
2040 1341 761
BclI T'GATC_A
Cuts at: 1662 1662
Size: 4142
BglI GCCn_nnn'nGGC
Cuts at: 649 649
Size: 4142
BglII A'GATC_T
Cuts at: 617 1657 3694 617
Size: 1040 2037 1065
Fragments arranged by size:
2037 1065 1040
BlpI GC'TnA_GC
Cuts at: 1254 1254
Size: 4142
Bme1580I G_kGCm'C
Cuts at: 171 682 810 1767 1860 2916 171
Size: 511 128 957 93 1056 1397
Fragments arranged by size:
1397 1056 957 511 128 93
BmgBI CAC'GTC
Cuts at: 876 876
Size: 4142
BmrI ACTGGGnnnn_n'
Cuts at: 1354 1435 1770 3914 1354
Size: 81 335 2144 1582
Fragments arranged by size:
2144 1582 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 4142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 694 730 968 1112 463
Size: 231 36 238 144 3493
Fragments arranged by size:
3493 238 231 144 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 2197 2689 2987 1318
Size: 879 492 298 2473
Fragments arranged by size:
2473 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 178 883 3846 178
Size: 705 2963 474
Fragments arranged by size:
2963 705 474
BsaAI yAC'GTr
Cuts at: 276 683 759 968 1495 276
Size: 407 76 209 527 2923
Fragments arranged by size:
2923 527 407 209 76
BsaBI GATnn'nnATC
Cuts at: 6 1656 3693 6
Size: 1650 2037 455
Fragments arranged by size:
2037 1650 455
BsaHI Gr'CG_yC
Cuts at: 115 136 340 516 1573 1821 2523 4019 115
Size: 21 204 176 1057 248 702 1496 238
Fragments arranged by size:
1496 1057 702 248 238 204 176 21
BsaWI w'CCGG_w
Cuts at: 1331 1852 2530 2804 2951 3362 1331
Size: 521 678 274 147 411 2111
Fragments arranged by size:
2111 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 506 536 571 601 667 697 1085 1115 506
Size: 30 35 30 66 30 388 30 3533
Fragments arranged by size:
3533 388 66 35 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 394 511 967 394
Size: 117 456 3569
Fragments arranged by size:
3569 456 117
BseYI C'CCAG_C
Cuts at: 803 1567 2325 2902 3398 803
Size: 764 758 577 496 1547
Fragments arranged by size:
1547 764 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 997
Size: 4142
BsiEI CG_ry'CG
Cuts at: 104 243 1103 1684 1730 2938 104
Size: 139 860 581 46 1208 1308
Fragments arranged by size:
1308 1208 860 581 139 46
BsiHKAI G_wGCw'C
Cuts at: 884 1132 1156 1934 2124 2916 884
Size: 248 24 778 190 792 2110
Fragments arranged by size:
2110 792 778 248 190 24
Bsp1286I G_dGCh'C
Cuts at: 171 199 254 682 810 884 1001 1132 1156
Size: 28 55 428 128 74 117 131 24
Cuts at: 1156 1767 1860 1934 2124 2186 2916 171
Size: 611 93 74 190 62 730 1397
Fragments arranged by size:
1397 730 611 428 190 131 128 117
93 74 74 62 55 28 24
BspEI T'CCGG_A
Cuts at: 3362 3362
Size: 4142
BspHI T'CATG_A
Cuts at: 3318 3318
Size: 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 434 590 1192 1406 1708 2089 434
Size: 156 602 214 302 381 2487
Fragments arranged by size:
2487 602 381 302 214 156
BsrBI CCG'CTC
Cuts at: 50 2434 2488 3972 4137 50
Size: 2384 54 1484 165 55
Fragments arranged by size:
2384 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2054 2054
Size: 4142
BsrFI r'CCGG_y
Cuts at: 2140 2321 2530 2140
Size: 181 209 3752
Fragments arranged by size:
3752 209 181
BssHII G'CGCG_C
Cuts at: 735 735
Size: 4142
BssSI C'ACGA_G
Cuts at: 883 2413 2771 883
Size: 1530 358 2254
Fragments arranged by size:
2254 1530 358
BstAPI GCAn_nnn'nTGC
Cuts at: 294 435 1519 294
Size: 141 1084 2917
Fragments arranged by size:
2917 1084 141
BstBI TT'CG_AA
Cuts at: 2503 2503
Size: 4142
BstYI r'GATC_y
Cuts at: 1 617 1167 1634 1657 1992 2238 3250 3336
Size: 616 550 467 23 335 246 1012 86
Cuts at: 3336 3348 3694 1
Size: 12 346 449
Fragments arranged by size:
1012 616 550 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 796 796
Size: 4142
BtgI C'CryG_G
Cuts at: 243 266 334 343 525 243
Size: 23 68 9 182 3860
Fragments arranged by size:
3860 182 68 23 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 246 2092 2273 2436 246
Size: 1846 181 163 1952
Fragments arranged by size:
1952 1846 181 163
BtsI GCAGTG_nn'
Cuts at: 605 1385 605
Size: 780 3362
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 107 142 107
Size: 35 4107
DraI TTT'AAA
Cuts at: 1141 1141
Size: 4142
DrdI GACnn_nn'nnGTC
Cuts at: 1848 2706 1848
Size: 858 3284
EaeI y'GGCC_r
Cuts at: 240 641 1727 1901 2292 2319 3664 240
Size: 401 1086 174 391 27 1345 718
Fragments arranged by size:
1345 1086 718 401 391 174 27
EagI C'GGCC_G
Cuts at: 240 1727 240
Size: 1487 2655
EarI CTCTTCn'nnn_
Cuts at: 154 371 1120 2165 2375 154
Size: 217 749 1045 210 1921
Fragments arranged by size:
1921 1045 749 217 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2350 2660 2806 3952 2350
Size: 310 146 1146 2540
Fragments arranged by size:
2540 1146 310 146
EcoICRI GAG'CTC
Cuts at: 882 1130 1154 882
Size: 248 24 3870
Fragments arranged by size:
3870 248 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 694 730 968 1112 1967 2399 3146 3414
Size: 231 36 238 144 855 432 747 268
Cuts at: 3414 463
Size: 1191
Fragments arranged by size:
1191 855 747 432 268 238 231 144
36
EcoNI CCTnn'n_nnAGG
Cuts at: 574 574
Size: 4142
EcoO109I rG'GnC_Cy
Cuts at: 167 168 579 807 1281 167
Size: 1 411 228 474 3028
Fragments arranged by size:
3028 474 411 228 1
EcoRI G'AATT_C
Cuts at: 1146 1146
Size: 4142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 4142
FspI TGC'GCA
Cuts at: 1923 1923
Size: 4142
HaeII r_GCGC'y
Cuts at: 139 224 343 1576 1824 2846 3825 139
Size: 85 119 1233 248 1022 979 456
Fragments arranged by size:
1233 1022 979 456 248 119 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 490 506 522 538 571 603 1085
Size: 32 163 16 16 16 33 32 482
Cuts at: 1085 1117 1984 2014 2016 2046 295
Size: 32 867 30 2 30 2391
Fragments arranged by size:
2391 867 482 163 33 32 32 32
30 30 16 16 16 2
HincII GTy'rAC
Cuts at: 1181 1181
Size: 4142
KasI G'GCGC_C
Cuts at: 135 339 1572 1820 135
Size: 204 1233 248 2457
Fragments arranged by size:
2457 1233 248 204
KpnI G_GTAC'C
Cuts at: 1162 1162
Size: 4142
MluI A'CGCG_T
Cuts at: 3575 3575
Size: 4142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 533 2813 2997 533
Size: 2280 184 1678
Fragments arranged by size:
2280 1678 184
MscI TGG'CCA
Cuts at: 1903 1903
Size: 4142
MslI CAynn'nnrTG
Cuts at: 265 685 2131 2258 265
Size: 420 1446 127 2149
Fragments arranged by size:
2149 1446 420 127
MspA1I CmG'CkG
Cuts at: 245 336 527 601 849 931 1081 1253 1567
Size: 91 191 74 248 82 150 172 314
Cuts at: 1567 1927 2940 3185 245
Size: 360 1013 245 1202
Fragments arranged by size:
1202 1013 360 314 248 245 191 172
150 91 82 74
NaeI GCC'GGC
Cuts at: 2323 2323
Size: 4142
NarI GG'CG_CC
Cuts at: 136 340 1573 1821 136
Size: 204 1233 248 2457
Fragments arranged by size:
2457 1233 248 204
NcoI C'CATG_G
Cuts at: 266 266
Size: 4142
NgoMIV G'CCGG_C
Cuts at: 2321 2321
Size: 4142
NspI r_CATG'y
Cuts at: 1195 1695 2602 1195
Size: 500 907 2735
Fragments arranged by size:
2735 907 500
PasI CC'CwG_GG
Cuts at: 810 810
Size: 4142
PciI A'CATG_T
Cuts at: 2598 2598
Size: 4142
PflMI CCAn_nnn'nTGG
Cuts at: 876 876
Size: 4142
PfoI T'CCnGG_A
Cuts at: 589 589
Size: 4142
PmeI GTTT'AAAC
Cuts at: 1141 1141
Size: 4142
PmlI CAC'GTG
Cuts at: 683 759 968 683
Size: 76 209 3857
Fragments arranged by size:
3857 209 76
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 4110
PpuMI rG'GwC_Cy
Cuts at: 579 579
Size: 4142
PspOMI G'GGCC_C
Cuts at: 167 806 167
Size: 639 3503
PstI C_TGCA'G
Cuts at: 431 734 903 1189 1874 431
Size: 303 169 286 685 2699
Fragments arranged by size:
2699 685 303 286 169
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4142
PvuII CAG'CTG
Cuts at: 931 1081 1567 1927 931
Size: 150 486 360 3146
Fragments arranged by size:
3146 486 360 150
RsrII CG'GwC_CG
Cuts at: 2337 2337
Size: 4142
SacI G_AGCT'C
Cuts at: 884 1132 1156 884
Size: 248 24 3870
Fragments arranged by size:
3870 248 24
SacII CC_GC'GG
Cuts at: 246 337 528 246
Size: 91 191 3860
Fragments arranged by size:
3860 191 91
SalI G'TCGA_C
Cuts at: 1179 1179
Size: 4142
SapI GCTCTTCn'nnn_
Cuts at: 1120 2165 2375 1120
Size: 1045 210 2887
Fragments arranged by size:
2887 1045 210
SbfI CC_TGCA'GG
Cuts at: 903 1189 903
Size: 286 3856
ScaI AGT'ACT
Cuts at: 3659 3659
Size: 4142
SfcI C'TryA_G
Cuts at: 33 427 730 899 1082 1185 1870 2863 3054
Size: 394 303 169 183 103 685 993 191
Cuts at: 3054 33
Size: 1121
Fragments arranged by size:
1121 993 685 394 303 191 183 169
103
SfoI GGC'GCC
Cuts at: 137 341 1574 1822 137
Size: 204 1233 248 2457
Fragments arranged by size:
2457 1233 248 204
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
SmaI CCC'GGG
Cuts at: 1164 1164
Size: 4142
SmlI C'TyrA_G
Cuts at: 1297 2212 2704 2966 1297
Size: 915 492 262 2473
Fragments arranged by size:
2473 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4142
SphI G_CATG'C
Cuts at: 1195 1695 1195
Size: 500 3642
StuI AGG'CCT
Cuts at: 897 897
Size: 4142
StyI C'CwwG_G
Cuts at: 118 266 395 1276 1630 118
Size: 148 129 881 354 2630
Fragments arranged by size:
2630 881 354 148 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 196 336 1725 1858 2317 2526 196
Size: 140 1389 133 459 209 1812
Fragments arranged by size:
1812 1389 459 209 140 133
TatI w'GTAC_w
Cuts at: 3657 3657
Size: 4142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 667 699 3659 3691 667
Size: 32 2960 32 1118
Fragments arranged by size:
2960 1118 32 32
Tth111I GACn'n_nGTC
Cuts at: 1939 1939
Size: 4142
XbaI T'CTAG_A
Cuts at: 1173 1173
Size: 4142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 273 487 1044 1637 273
Size: 214 557 593 2778
Fragments arranged by size:
2778 593 557 214
XmaI C'CCGG_G
Cuts at: 1162 1162
Size: 4142
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI