Restriction map for
pF1KB6431
(Circular) (Six-base) MAPSORT of: pF1KB6431.seq Check: 6923 from: 1 to: 4049
pF1KB6431 4049 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1087 1087
Size: 4049
Acc65I G'GTAC_C
Cuts at: 494 1065 494
Size: 571 3478
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
AflIII A'CryG_T
Cuts at: 1401 2505 3482 1401
Size: 1104 977 1968
Fragments arranged by size:
1968 1104 977
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AleI CACnn'nnGTG
Cuts at: 707 829 707
Size: 122 3927
AlwNI CAG_nnn'CTG
Cuts at: 297 504 567 727 1354 2921 297
Size: 207 63 160 627 1567 1425
Fragments arranged by size:
1567 1425 627 207 160 63
ApaI G_GGCC'C
Cuts at: 764 764
Size: 4049
ApaLI G'TGCA_C
Cuts at: 832 985 2819 832
Size: 153 1834 2062
Fragments arranged by size:
2062 1834 153
ApoI r'AATT_y
Cuts at: 1053 3402 3658 1053
Size: 2349 256 1444
Fragments arranged by size:
2349 1444 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 181 214 1069 181
Size: 33 855 3161
Fragments arranged by size:
3161 855 33
AvrII C'CTAG_G
Cuts at: 919 919
Size: 4049
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 478 511 478
Size: 33 4016
BamHI G'GATC_C
Cuts at: 1074 1074
Size: 4049
BanI G'GyrC_C
Cuts at: 357 494 553 1065 1479 1727 1762 357
Size: 137 59 512 414 248 35 2644
Fragments arranged by size:
2644 512 414 248 137 59 35
BanII G_rGCy'C
Cuts at: 215 319 350 374 764 1063 2093 215
Size: 104 31 24 390 299 1030 2171
Fragments arranged by size:
2171 1030 390 299 104 31 24
BbeI G_GCGC'C
Cuts at: 557 1483 1731 557
Size: 926 248 2875
Fragments arranged by size:
2875 926 248
BbvCI CC'TCA_GC
Cuts at: 853 853
Size: 4049
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 1569 1569
Size: 4049
BglI GCCn_nnn'nGGC
Cuts at: 730 730
Size: 4049
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 344 1161 344
Size: 817 3232
Bme1580I G_kGCm'C
Cuts at: 477 764 802 836 989 1674 1767 2823 477
Size: 287 38 34 153 685 93 1056 1703
Fragments arranged by size:
1703 1056 685 287 153 93 38 34
BmrI ACTGGGnnnn_n'
Cuts at: 1261 1342 1677 3821 1261
Size: 81 335 2144 1489
Fragments arranged by size:
2144 1489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 507 672 925 453
Size: 54 165 253 3577
Fragments arranged by size:
3577 253 165 54
Bpu10I CC'TnA_GC
Cuts at: 853 853
Size: 4049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 337 1225 2104 2596 2894 162
Size: 175 888 879 492 298 1317
Fragments arranged by size:
1317 888 879 492 298 175
BsaI GGTCTCn'nnnn_
Cuts at: 883 1045 3753 883
Size: 162 2708 1179
Fragments arranged by size:
2708 1179 162
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 554 1480 1728 2430 3926 554
Size: 926 248 702 1496 677
Fragments arranged by size:
1496 926 702 677 248
BsaWI w'CCGG_w
Cuts at: 224 1238 1759 2437 2711 2858 3269 224
Size: 1014 521 678 274 147 411 1004
Fragments arranged by size:
1014 1004 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 669 901 922 223
Size: 446 232 21 3350
Fragments arranged by size:
3350 446 232 21
BseYI C'CCAG_C
Cuts at: 187 621 720 1474 2232 2809 3305 187
Size: 434 99 754 758 577 496 931
Fragments arranged by size:
931 758 754 577 496 434 99
BsiEI CG_ry'CG
Cuts at: 104 1031 1591 1637 2845 104
Size: 927 560 46 1208 1308
Fragments arranged by size:
1308 1208 927 560 46
BsiHKAI G_wGCw'C
Cuts at: 458 836 989 1063 1841 2031 2823 458
Size: 378 153 74 778 190 792 1684
Fragments arranged by size:
1684 792 778 378 190 153 74
Bsp1286I G_dGCh'C
Cuts at: 215 319 350 374 458 477 764 802 836
Size: 104 31 24 84 19 287 38 34
Cuts at: 836 989 1063 1674 1767 1841 2031 2093 2823
Size: 153 74 611 93 74 190 62 730
Cuts at: 2823 215
Size: 1441
Fragments arranged by size:
1441 730 611 287 190 153 104 93
84 74 74 62 38 34 31 24
19
BspEI T'CCGG_A
Cuts at: 224 3269 224
Size: 3045 1004
BspHI T'CATG_A
Cuts at: 3225 3225
Size: 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 482 1099 1313 1615 1996 482
Size: 617 214 302 381 2535
Fragments arranged by size:
2535 617 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 812 1961 812
Size: 1149 2900
BsrFI r'CCGG_y
Cuts at: 2047 2228 2437 2047
Size: 181 209 3659
Fragments arranged by size:
3659 209 181
BssSI C'ACGA_G
Cuts at: 934 2320 2678 934
Size: 1386 358 2305
Fragments arranged by size:
2305 1386 358
BstAPI GCAn_nnn'nTGC
Cuts at: 567 1426 567
Size: 859 3190
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstYI r'GATC_y
Cuts at: 1 1074 1541 1564 1899 2145 3157 3243 3255
Size: 1073 467 23 335 246 1012 86 12
Cuts at: 3255 3601 1
Size: 346 449
Fragments arranged by size:
1073 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 193 280 193
Size: 87 3962
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 456 824 1292 456
Size: 368 468 3213
Fragments arranged by size:
3213 468 368
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 731 963 1028 1634 1808 2199 2226 3571 731
Size: 232 65 606 174 391 27 1345 1209
Fragments arranged by size:
1345 1209 606 391 232 174 65 27
EagI C'GGCC_G
Cuts at: 1028 1634 1028
Size: 606 3443
EarI CTCTTCn'nnn_
Cuts at: 225 2072 2282 225
Size: 1847 210 1992
Fragments arranged by size:
1992 1847 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 507 672 925 1874 2306 3053 3321 453
Size: 54 165 253 949 432 747 268 1181
Fragments arranged by size:
1181 949 747 432 268 253 165 54
EcoO109I rG'GnC_Cy
Cuts at: 178 579 1188 178
Size: 401 609 3039
Fragments arranged by size:
3039 609 401
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 557 689 748 1483 1731 2753 3732 557
Size: 132 59 735 248 1022 979 874
Fragments arranged by size:
1022 979 874 735 248 132 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1891 1921 1923 1953 1891
Size: 30 2 30 3987
Fragments arranged by size:
3987 30 30 2
HincII GTy'rAC
Cuts at: 399 1088 399
Size: 689 3360
KasI G'GCGC_C
Cuts at: 553 1479 1727 553
Size: 926 248 2875
Fragments arranged by size:
2875 926 248
KpnI G_GTAC'C
Cuts at: 498 1069 498
Size: 571 3478
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2904 2720
Size: 184 3865
MscI TGG'CCA
Cuts at: 1810 1810
Size: 4049
MslI CAynn'nnrTG
Cuts at: 461 707 817 829 2038 2165 461
Size: 246 110 12 1209 127 2345
Fragments arranged by size:
2345 1209 246 127 110 12
MspA1I CmG'CkG
Cuts at: 195 282 393 1160 1474 1834 2847 3092 195
Size: 87 111 767 314 360 1013 245 1152
Fragments arranged by size:
1152 1013 767 360 314 245 111 87
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 554 1480 1728 554
Size: 926 248 2875
Fragments arranged by size:
2875 926 248
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NspI r_CATG'y
Cuts at: 832 1102 1602 2509 832
Size: 270 500 907 2372
Fragments arranged by size:
2372 907 500 270
PasI CC'CwG_GG
Cuts at: 1035 1035
Size: 4049
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PpuMI rG'GwC_Cy
Cuts at: 178 178
Size: 4049
PspOMI G'GGCC_C
Cuts at: 760 760
Size: 4049
PstI C_TGCA'G
Cuts at: 243 605 698 1096 1781 243
Size: 362 93 398 685 2511
Fragments arranged by size:
2511 685 398 362 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 393 1474 1834 393
Size: 1081 360 2608
Fragments arranged by size:
2608 1081 360
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SacII CC_GC'GG
Cuts at: 196 283 196
Size: 87 3962
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SanDI GG'GwC_CC
Cuts at: 178 178
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SexAI A'CCwGG_T
Cuts at: 497 539 497
Size: 42 4007
SfcI C'TryA_G
Cuts at: 33 239 601 694 1092 1777 2770 2961 33
Size: 206 362 93 398 685 993 191 1121
Fragments arranged by size:
1121 993 685 398 362 206 191 93
SfoI GGC'GCC
Cuts at: 555 1481 1729 555
Size: 926 248 2875
Fragments arranged by size:
2875 926 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 183 216 1071 183
Size: 33 855 3161
Fragments arranged by size:
3161 855 33
SmlI C'TyrA_G
Cuts at: 141 352 1204 2119 2611 2873 141
Size: 211 852 915 492 262 1317
Fragments arranged by size:
1317 915 852 492 262 211
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
SrfI GCCC'GGGC
Cuts at: 216 216
Size: 4049
StyI C'CwwG_G
Cuts at: 919 1183 1537 919
Size: 264 354 3431
Fragments arranged by size:
3431 354 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1632 1765 2224 2433 1632
Size: 133 459 209 3248
Fragments arranged by size:
3248 459 209 133
TatI w'GTAC_w
Cuts at: 3564 3564
Size: 4049
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 938 970 3566 3598 938
Size: 32 2596 32 1389
Fragments arranged by size:
2596 1389 32 32
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 181 214 1069 181
Size: 33 855 3161
Fragments arranged by size:
3161 855 33
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
BbsI BcgI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PshAI PsiI PspXI PsrI
SfiI SgrAI SnaBI SspI StuI SwaI XhoI XmnI
ZraI