Restriction map for
pF1KB6432
(Circular) (Six-base) MAPSORT of: pF1KB6432.seq Check: 4864 from: 1 to: 4145
pF1KB6432 4145 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1183 1183
Size: 4145
Acc65I G'GTAC_C
Cuts at: 1161 1161
Size: 4145
AclI AA'CG_TT
Cuts at: 3985 3985
Size: 4145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 806 1970 2402 3149 3417 358
Size: 448 1164 432 747 268 1086
Fragments arranged by size:
1164 1086 747 448 432 268
AflIII A'CryG_T
Cuts at: 1497 2601 3578 1497
Size: 1104 977 2064
Fragments arranged by size:
2064 1104 977
AgeI A'CCGG_T
Cuts at: 2533 2533
Size: 4145
AhdI GACnn_n'nnGTC
Cuts at: 899 899
Size: 4145
AlwNI CAG_nnn'CTG
Cuts at: 1450 3017 1450
Size: 1567 2578
ApaLI G'TGCA_C
Cuts at: 326 2915 326
Size: 2589 1556
ApoI r'AATT_y
Cuts at: 460 649 791 1149 3498 3754 460
Size: 189 142 358 2349 256 851
Fragments arranged by size:
2349 851 358 256 189 142
AseI AT'TA_AT
Cuts at: 20 20
Size: 4145
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
AvaI C'yCGr_G
Cuts at: 135 185 1165 135
Size: 50 980 3115
Fragments arranged by size:
3115 980 50
BamHI G'GATC_C
Cuts at: 1170 1170
Size: 4145
BanI G'GyrC_C
Cuts at: 1161 1575 1823 1858 1161
Size: 414 248 35 3448
Fragments arranged by size:
3448 414 248 35
BanII G_rGCy'C
Cuts at: 1159 2189 1159
Size: 1030 3115
BbeI G_GCGC'C
Cuts at: 1579 1827 1579
Size: 248 3897
BciVI GTATCCnnnnn_n'
Cuts at: 2043 2804 4145 2043
Size: 761 1341 2043
Fragments arranged by size:
2043 1341 761
BclI T'GATC_A
Cuts at: 1665 1665
Size: 4145
BglII A'GATC_T
Cuts at: 1660 3697 1660
Size: 2037 2108
BlpI GC'TnA_GC
Cuts at: 1257 1257
Size: 4145
Bme1580I G_kGCm'C
Cuts at: 330 1077 1770 1863 2919 330
Size: 747 693 93 1056 1556
Fragments arranged by size:
1556 1056 747 693 93
BmrI ACTGGGnnnn_n'
Cuts at: 1357 1438 1773 3917 1357
Size: 81 335 2144 1585
Fragments arranged by size:
2144 1585 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 211 2512 211
Size: 2301 1844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 271 178
Size: 93 4052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1321 2200 2692 2990 961
Size: 360 879 492 298 2116
Fragments arranged by size:
2116 879 492 360 298
BsaI GGTCTCn'nnnn_
Cuts at: 3849 3849
Size: 4145
BsaAI yAC'GTr
Cuts at: 738 1498 738
Size: 760 3385
BsaBI GATnn'nnATC
Cuts at: 6 671 1659 3696 6
Size: 665 988 2037 455
Fragments arranged by size:
2037 988 665 455
BsaHI Gr'CG_yC
Cuts at: 1576 1824 2526 4022 1576
Size: 248 702 1496 1699
Fragments arranged by size:
1699 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1334 1855 2533 2807 2954 3365 1334
Size: 521 678 274 147 411 2114
Fragments arranged by size:
2114 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1073 1103 1073
Size: 30 4115
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 406 1081 406
Size: 675 3470
BseYI C'CCAG_C
Cuts at: 1570 2328 2905 3401 1570
Size: 758 577 496 2314
Fragments arranged by size:
2314 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1687 1733 2941 104
Size: 1583 46 1208 1308
Fragments arranged by size:
1583 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 197 330 1159 1937 2127 2919 197
Size: 133 829 778 190 792 1423
Fragments arranged by size:
1423 829 792 778 190 133
BsmI GAATG_Cn'
Cuts at: 301 301
Size: 4145
Bsp1286I G_dGCh'C
Cuts at: 197 330 1077 1159 1770 1863 1937 2127 2189
Size: 133 747 82 611 93 74 190 62
Cuts at: 2189 2919 197
Size: 730 1423
Fragments arranged by size:
1423 747 730 611 190 133 93 82
74 62
BspEI T'CCGG_A
Cuts at: 3365 3365
Size: 4145
BspHI T'CATG_A
Cuts at: 3321 3321
Size: 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1195 1409 1711 2092 1195
Size: 214 302 381 3248
Fragments arranged by size:
3248 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2437 2491 3975 4140 50
Size: 2387 54 1484 165 55
Fragments arranged by size:
2387 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 313 663 2057 313
Size: 350 1394 2401
Fragments arranged by size:
2401 1394 350
BsrFI r'CCGG_y
Cuts at: 2143 2324 2533 2143
Size: 181 209 3755
Fragments arranged by size:
3755 209 181
BssSI C'ACGA_G
Cuts at: 2416 2774 2416
Size: 358 3787
BstAPI GCAn_nnn'nTGC
Cuts at: 1522 1522
Size: 4145
BstBI TT'CG_AA
Cuts at: 2506 2506
Size: 4145
BstYI r'GATC_y
Cuts at: 1 533 1170 1637 1660 1995 2241 3253 3339
Size: 532 637 467 23 335 246 1012 86
Cuts at: 3339 3351 3697 1
Size: 12 346 449
Fragments arranged by size:
1012 637 532 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 119 1118 107
Size: 12 999 3134
Fragments arranged by size:
3134 999 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2095 2276 2439 2095
Size: 181 163 3801
Fragments arranged by size:
3801 181 163
BtsI GCAGTG_nn'
Cuts at: 1388 1388
Size: 4145
ClaI AT'CG_AT
Cuts at: 272 272
Size: 4145
DraI TTT'AAA
Cuts at: 729 1144 729
Size: 415 3730
DrdI GACnn_nn'nnGTC
Cuts at: 1851 2709 1851
Size: 858 3287
EaeI y'GGCC_r
Cuts at: 1730 1904 2295 2322 3667 1730
Size: 174 391 27 1345 2208
Fragments arranged by size:
2208 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1730 1730
Size: 4145
EarI CTCTTCn'nnn_
Cuts at: 407 2168 2378 407
Size: 1761 210 2174
Fragments arranged by size:
2174 1761 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2353 2663 2809 3955 2353
Size: 310 146 1146 2543
Fragments arranged by size:
2543 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1157 1157
Size: 4145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 271 358 806 1970 2402 3149 3417 178
Size: 93 87 448 1164 432 747 268 906
Fragments arranged by size:
1164 906 747 448 432 268 93 87
EcoNI CCTnn'n_nnAGG
Cuts at: 481 481
Size: 4145
EcoO109I rG'GnC_Cy
Cuts at: 1284 1284
Size: 4145
EcoRI G'AATT_C
Cuts at: 791 1149 791
Size: 358 3787
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 4145
FspI TGC'GCA
Cuts at: 1926 1926
Size: 4145
HaeII r_GCGC'y
Cuts at: 1579 1827 2849 3828 1579
Size: 248 1022 979 1896
Fragments arranged by size:
1896 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 885 917 1987 2017 2019 2049 885
Size: 32 1070 30 2 30 2981
Fragments arranged by size:
2981 1070 32 30 30 2
HincII GTy'rAC
Cuts at: 1184 1184
Size: 4145
HindIII A'AGCT_T
Cuts at: 996 996
Size: 4145
KasI G'GCGC_C
Cuts at: 1575 1823 1575
Size: 248 3897
KpnI G_GTAC'C
Cuts at: 1165 1165
Size: 4145
MluI A'CGCG_T
Cuts at: 3578 3578
Size: 4145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 210 2816 3000 210
Size: 2606 184 1355
Fragments arranged by size:
2606 1355 184
MscI TGG'CCA
Cuts at: 1906 1906
Size: 4145
MslI CAynn'nnrTG
Cuts at: 2134 2261 2134
Size: 127 4018
MspA1I CmG'CkG
Cuts at: 143 1256 1570 1930 2943 3188 143
Size: 1113 314 360 1013 245 1100
Fragments arranged by size:
1113 1100 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2326 2326
Size: 4145
NarI GG'CG_CC
Cuts at: 1576 1824 1576
Size: 248 3897
NcoI C'CATG_G
Cuts at: 107 1118 107
Size: 1011 3134
NgoMIV G'CCGG_C
Cuts at: 2324 2324
Size: 4145
NspI r_CATG'y
Cuts at: 825 1198 1698 2605 825
Size: 373 500 907 2365
Fragments arranged by size:
2365 907 500 373
PciI A'CATG_T
Cuts at: 2601 2601
Size: 4145
PmeI GTTT'AAAC
Cuts at: 1144 1144
Size: 4145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 4113
PstI C_TGCA'G
Cuts at: 1192 1877 1192
Size: 685 3460
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4145
PvuII CAG'CTG
Cuts at: 1570 1930 1570
Size: 360 3785
RsrII CG'GwC_CG
Cuts at: 2340 2340
Size: 4145
SacI G_AGCT'C
Cuts at: 1159 1159
Size: 4145
SalI G'TCGA_C
Cuts at: 1182 1182
Size: 4145
SapI GCTCTTCn'nnn_
Cuts at: 2168 2378 2168
Size: 210 3935
SbfI CC_TGCA'GG
Cuts at: 1192 1192
Size: 4145
ScaI AGT'ACT
Cuts at: 3662 3662
Size: 4145
SfcI C'TryA_G
Cuts at: 33 848 1188 1873 2866 3057 33
Size: 815 340 685 993 191 1121
Fragments arranged by size:
1121 993 815 685 340 191
SfoI GGC'GCC
Cuts at: 1577 1825 1577
Size: 248 3897
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
SmaI CCC'GGG
Cuts at: 1167 1167
Size: 4145
SmlI C'TyrA_G
Cuts at: 940 1300 2215 2707 2969 940
Size: 360 915 492 262 2116
Fragments arranged by size:
2116 915 492 360 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4145
SphI G_CATG'C
Cuts at: 825 1198 1698 825
Size: 373 500 3272
Fragments arranged by size:
3272 500 373
SspI AAT'ATT
Cuts at: 870 982 870
Size: 112 4033
StyI C'CwwG_G
Cuts at: 107 1118 1279 1633 107
Size: 1011 161 354 2619
Fragments arranged by size:
2619 1011 354 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1728 1861 2320 2529 1728
Size: 133 459 209 3344
Fragments arranged by size:
3344 459 209 133
TatI w'GTAC_w
Cuts at: 3660 3660
Size: 4145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3662 3694 3662
Size: 32 4113
Tth111I GACn'n_nGTC
Cuts at: 1942 1942
Size: 4145
XbaI T'CTAG_A
Cuts at: 748 1176 748
Size: 428 3717
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1640 1640
Size: 4145
XmaI C'CCGG_G
Cuts at: 1165 1165
Size: 4145
XmnI GAAnn'nnTTC
Cuts at: 206 554 791 983 206
Size: 348 237 192 3368
Fragments arranged by size:
3368 348 237 192
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI