Restriction map for
pF1KB6433
(Circular) (Six-base) MAPSORT of: pF1KB6433.seq Check: 7134 from: 1 to: 4052
pF1KB6433 4052 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 373 373
Size: 4052
AccI GT'mk_AC
Cuts at: 1090 1090
Size: 4052
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 1877 2309 3056 3324 315
Size: 1562 432 747 268 1043
Fragments arranged by size:
1562 1043 747 432 268
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AleI CACnn'nnGTG
Cuts at: 547 718 547
Size: 171 3881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 381 381
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaI G_GGCC'C
Cuts at: 524 883 899 524
Size: 359 16 3677
Fragments arranged by size:
3677 359 16
ApaLI G'TGCA_C
Cuts at: 1019 2822 1019
Size: 1803 2249
ApoI r'AATT_y
Cuts at: 421 1056 3405 3661 421
Size: 635 2349 256 812
Fragments arranged by size:
2349 812 635 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 702 826 844 1072 702
Size: 124 18 228 3682
Fragments arranged by size:
3682 228 124 18
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 376 820 840 1068 1482 1730 1765 376
Size: 444 20 228 414 248 35 2663
Fragments arranged by size:
2663 444 414 248 228 35 20
BanII G_rGCy'C
Cuts at: 182 260 471 524 883 899 1066 2096 182
Size: 78 211 53 359 16 167 1030 2138
Fragments arranged by size:
2138 1030 359 211 167 78 53 16
BbeI G_GCGC'C
Cuts at: 824 1486 1734 824
Size: 662 248 3142
Fragments arranged by size:
3142 662 248
BbsI GAAGACnn'nnnn_
Cuts at: 204 381 204
Size: 177 3875
BbvCI CC'TCA_GC
Cuts at: 301 301
Size: 4052
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 294 328 90
Size: 34 170 34 3814
Fragments arranged by size:
3814 170 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BglI GCCn_nnn'nGGC
Cuts at: 374 374
Size: 4052
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 265 1164 265
Size: 899 3153
Bme1580I G_kGCm'C
Cuts at: 379 524 883 899 1023 1677 1770 2826 379
Size: 145 359 16 124 654 93 1056 1605
Fragments arranged by size:
1605 1056 654 359 145 124 93 16
BmgBI CAC'GTC
Cuts at: 132 132
Size: 4052
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 244
Size: 4052
Bpu10I CC'TnA_GC
Cuts at: 301 301
Size: 4052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 2107 2599 2897 1228
Size: 879 492 298 2383
Fragments arranged by size:
2383 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 683 3756 683
Size: 3073 979
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 821 851 1483 1731 2433 3929 821
Size: 30 632 248 702 1496 944
Fragments arranged by size:
1496 944 702 632 248 30
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 399 399
Size: 4052
BseYI C'CCAG_C
Cuts at: 151 788 1477 2235 2812 3308 151
Size: 637 689 758 577 496 895
Fragments arranged by size:
895 758 689 637 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 322
Size: 4052
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 260 749 1023 1066 1844 2034 2826 260
Size: 489 274 43 778 190 792 1486
Fragments arranged by size:
1486 792 778 489 274 190 43
Bsp1286I G_dGCh'C
Cuts at: 182 260 379 471 524 749 883 899 1023
Size: 78 119 92 53 225 134 16 124
Cuts at: 1023 1066 1677 1770 1844 2034 2096 2826 182
Size: 43 611 93 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 225 190 134 124 119
93 92 78 74 62 53 43 16
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 373 1102 1316 1618 1999 373
Size: 729 214 302 381 2426
Fragments arranged by size:
2426 729 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 211 1964 211
Size: 1753 2299
BsrFI r'CCGG_y
Cuts at: 837 952 2050 2231 2440 837
Size: 115 1098 181 209 2449
Fragments arranged by size:
2449 1098 209 181 115
BssHII G'CGCG_C
Cuts at: 807 809 807
Size: 2 4050
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 1044 1044
Size: 4052
BstYI r'GATC_y
Cuts at: 1 1077 1544 1567 1902 2148 3160 3246 3258
Size: 1076 467 23 335 246 1012 86 12
Cuts at: 3258 3604 1
Size: 346 449
Fragments arranged by size:
1076 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 917 980 917
Size: 63 3989
BtgI C'CryG_G
Cuts at: 434 461 542 434
Size: 27 81 3944
Fragments arranged by size:
3944 81 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 875 2002 2183 2346 875
Size: 1127 181 163 2581
Fragments arranged by size:
2581 1127 181 163
BtsI GCAGTG_nn'
Cuts at: 972 1295 972
Size: 323 3729
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DraIII CAC_nnn'GTG
Cuts at: 345 345
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 437 473 539 614 1637 1811 2202 2229 3574
Size: 36 66 75 1023 174 391 27 1345
Cuts at: 3574 437
Size: 915
Fragments arranged by size:
1345 1023 915 391 174 75 66 36
27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 136 2260 2570 2716 3862 136
Size: 2124 310 146 1146 326
Fragments arranged by size:
2124 1146 326 310 146
EcoICRI GAG'CTC
Cuts at: 258 1064 258
Size: 806 3246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 315 1877 2309 3056 3324 244
Size: 71 1562 432 747 268 972
Fragments arranged by size:
1562 972 747 432 268 71
EcoO109I rG'GnC_Cy
Cuts at: 520 879 880 896 1191 520
Size: 359 1 16 295 3381
Fragments arranged by size:
3381 359 295 16 1
EcoRI G'AATT_C
Cuts at: 421 1056 421
Size: 635 3417
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 408 2071 408
Size: 1663 2389
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 824 1486 1734 2756 3735 824
Size: 662 248 1022 979 1141
Fragments arranged by size:
1141 1022 979 662 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 394 426 1894 1924 1926 1956 394
Size: 32 1468 30 2 30 2490
Fragments arranged by size:
2490 1468 32 30 30 2
HincII GTy'rAC
Cuts at: 946 1091 946
Size: 145 3907
KasI G'GCGC_C
Cuts at: 820 1482 1730 820
Size: 662 248 3142
Fragments arranged by size:
3142 662 248
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2723 2907 2723
Size: 184 3868
MscI TGG'CCA
Cuts at: 475 541 616 1813 475
Size: 66 75 1197 2714
Fragments arranged by size:
2714 1197 75 66
MslI CAynn'nnrTG
Cuts at: 130 547 582 718 2041 2168 130
Size: 417 35 136 1323 127 2014
Fragments arranged by size:
2014 1323 417 136 127 35
MspA1I CmG'CkG
Cuts at: 144 264 959 1163 1477 1837 2850 3095 144
Size: 120 695 204 314 360 1013 245 1101
Fragments arranged by size:
1101 1013 695 360 314 245 204 120
NaeI GCC'GGC
Cuts at: 954 2233 954
Size: 1279 2773
NarI GG'CG_CC
Cuts at: 821 1483 1731 821
Size: 662 248 3142
Fragments arranged by size:
3142 662 248
NcoI C'CATG_G
Cuts at: 461 461
Size: 4052
NgoMIV G'CCGG_C
Cuts at: 952 2231 952
Size: 1279 2773
NspI r_CATG'y
Cuts at: 239 677 1105 1605 2512 239
Size: 438 428 500 907 1779
Fragments arranged by size:
1779 907 500 438 428
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 301 333 3191 3223 301
Size: 32 2858 32 1130
Fragments arranged by size:
2858 1130 32 32
PshAI GACnn'nnGTC
Cuts at: 941 941
Size: 4052
PspOMI G'GGCC_C
Cuts at: 520 879 895 520
Size: 359 16 3677
Fragments arranged by size:
3677 359 16
PstI C_TGCA'G
Cuts at: 494 1099 1784 494
Size: 605 685 2762
Fragments arranged by size:
2762 685 605
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 144 264 1477 1837 144
Size: 120 1213 360 2359
Fragments arranged by size:
2359 1213 360 120
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 260 1066 260
Size: 806 3246
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SexAI A'CCwGG_T
Cuts at: 740 740
Size: 4052
SfcI C'TryA_G
Cuts at: 33 246 490 1095 1780 2773 2964 33
Size: 213 244 605 685 993 191 1121
Fragments arranged by size:
1121 993 685 605 244 213 191
SfoI GGC'GCC
Cuts at: 822 1484 1732 822
Size: 662 248 3142
Fragments arranged by size:
3142 662 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 846 1074 846
Size: 228 3824
SmlI C'TyrA_G
Cuts at: 1207 2122 2614 2876 1207
Size: 915 492 262 2383
Fragments arranged by size:
2383 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StyI C'CwwG_G
Cuts at: 383 461 590 1186 1540 383
Size: 78 129 596 354 2895
Fragments arranged by size:
2895 596 354 129 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 304 375 1038 1635 1768 2227 2436 304
Size: 71 663 597 133 459 209 1920
Fragments arranged by size:
1920 663 597 459 209 133 71
TatI w'GTAC_w
Cuts at: 347 3567 347
Size: 3220 832
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 468 937 1547 114
Size: 354 469 610 2619
Fragments arranged by size:
2619 610 469 354
XmaI C'CCGG_G
Cuts at: 844 1072 844
Size: 228 3824
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BfrBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI